DJ-1/park7 modulates vasorelaxation and blood pressure via epigenetic modification of endothelial nitric oxide synthase

Won, Kyung Jong,Jung, Seung Hyo,Jung, Soo Hyun,Lee, Kang Pa,Lee, Hwan Myung,Lee, Dong-Youb,Park, Eun-Seok,Kim, Junghwan,Kim, Bokyung Oxford University Press 2014 Cardiovascular research Vol.101 No.3

<P><B>Aims</B></P><P>DJ-1/park7, a multifunctional protein, may play essential roles in the vascular system. However, the function of DJ-1/park7 in vascular contractility has remained unclear. The present study was designed to investigate whether the DJ-1/park7 is involved in the regulation of vascular contractility and systolic blood pressure (SBP).</P><P><B>Methods and results</B></P><P>Norepinephrine (NE) elevated contraction in endothelium-intact vessels in a dose-dependent manner, to a greater extent in DJ-1/park7 knockout (DJ-1/park7<SUP>−/−</SUP>) mice than in wild-type (DJ-1/park7<SUP>+/+</SUP>) mice. Acetylcholine inhibited NE-evoked contraction in endothelium-intact vessels, and this was markedly impaired in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP>. Nitric oxide (NO) production (82.1 ± 2.8% of control) and endothelial NO synthase (eNOS) expression (61.7 ± 8.9%) were lower, but H<SUB>2</SUB>O<SUB>2</SUB> production (126.4 ± 8.6%) was higher, in endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice than in those from DJ-1/park7<SUP>+/+</SUP> controls; these effects were reversed by DJ-1/park7-overexpressing endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice. Histone deacetylase (HDAC)-1 recruitment and H3 histone acetylation at the eNOS promoter were elevated and diminished, respectively, in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls. Moreover, SBP was significantly elevated in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls, but this elevation was inhibited in mice treated with valproic acid, an inhibitor of Class I HDACs including HDAC-1.</P><P><B>Conclusion</B></P><P>These results demonstrate that DJ-1/park7 protein may be implicated in the regulation of vascular contractility and blood pressure, probably by the impairment of NO production through H<SUB>2</SUB>O<SUB>2</SUB>-mediated epigenetic inhibition of eNOS expression.</P>

해상형 국립공원 탐방수요의 요인분해

모수원 ( Mo Soo-won ),이광배 ( Lee Kwang-bae ),박정환 ( Park Jeong-hwan ) 한국도서(섬)학회 2018 韓國島嶼硏究 Vol.30 No.2

국내관광은 국제관광의 변동성이 주는 충격을 완화하려 관광산업의 안정성을 담보하는 역할을 수행함에도 불구하고 국내관광부문은 활성화되지 못하고 있다. 이러한 점은 우리나라의 해외관광객은 2010년-2017년에 연평균 11.3%라는 높은 증가를 보인데 비해 우리나라 국립공원의 탐방객은 연평균 1.5% 성장이라는 초라한 실적을 드러내고 있다는 데서 알 수 있다. 더욱 산악형 국립공원에 대해서는 비교적 많은 연구가 이루어지고 있는데 비해 해상형 국립공원에 대해서는 상대적으로 연구와 관심이 빈약한 편이다. 이에 본고는 변이할당분석과 BCG분석을 이용하여 해상형 국립공원 탐방객의 변동요인을 식별한다. 먼저 변이할당분석을 적용하여 4개 해상형 국립공원 탐방객의 변동을 국립공원 전체에 대한 탐방수요가 개별 국립공원탐방수요에 미치는 국립공원효과, 국립공원 전체에 대한 매력과 해상형 국립공원에 대한 매력의 차이가 개별 국립공원 탐방수요에 미치는 해상형 국립공원효과, 그리고 개별 국립공원과 해상형 국립공원의 탐방수요가 미치는 소위 경쟁력효과와 같은 3개 요인으로 분해한다. 또한 변이할당분석을 정태적 분석과 동태적 분석으로 구분하여 정태적 분석과 동태적 분석의 3개효과가 부호와 크기에서 상당한 차이를 노정한다는 것도 밝힌다. 구체적으로 우리나라 4개 해상형 국립공원의 탐방객 변동을 요인별로 분해하여, 정태적 분석에서는 국가효과와 구성효과가 양의 값으로 4개 해상형 국립공원 탐방객을 증가시키나, 동태적 분석에서는 구성효과의 경우 2013년부터, 국가효과의 경우 2014년부터 음의 부호를 갖는다는 것을 밝힌다. 탐방객이 증가한 오동도, 다도해해상국립공원, 태안해안국립공원은 물론 탐방객이 감소한 변산반도국립공원에서도 개별 국립공원의 경쟁력을 의미하는 지역효과가 가장 큰 영향을 미친다는 것도 보인다. BCG 매트릭스를 적용하여 오동도와 다도해해상국립공원은 star 영역으로 상승한 후star 위상을 유지하며, 변산반도국립공원은 star에서 cash cow와 child로 위상이 변화하여 탐방객 증가가 평균 이상을 유지하고 있는데 비해 태안해안국립공원은 child에서 dog로 추락함을 보인다. 이러한 분석을 통해 4개의 해상형 국립공원 모두에서 지역효과가 탐방객 변동에 가장 큰 영향을 미친다는 것과, 오동도와 다도해해상국립공원의 경쟁력은 우수하나 변산반도 국립공원의 경쟁력은 정체상태에 있으며, 태안해안국립공원의 경쟁력은 하락하고 있음을 밝힌다. BCG분석에서는 오동도와 다도해해상국립공원이 탐방객에서 경쟁력이 있다는 것과 태안해안국립공원에서 가장 큰 경쟁력 향상이 이루어져야 한다는 것도 밝힌다. Tourism is widely considered one of the world's largest and rapidly growing industries. Domestic tourism, however, remains an under-researched theme in many countries involving Korea partly due to the difficulty to track domestic tourism and with governments and policy-makers placing emphasis on the attractive higher expenditures of foreign visitors. It is stated that a vibrant domestic tourism sector can cushion the industry from fluctuations of the international tourism market and bring stability and predictability in the industry. Nature-based tourism to national parks gives us broad benefits such as improved physical and mental health, stress reduction and increased social cohesion. Employment is one of tourism’s main development advantages and its role in promoting economic opportunities for communities adjacent to national parks has long been appreciated. But tourism performance in Korean national parks continues to dwindle and stagger. This paper introduces a shift-share decomposition to break down the tourism to the marine national parks into three components: one measuring national effect, and two other components due to changes in compositional effect and regional effect respectively. While the static shift-share analysis shows that the growth in marine national parks’tourism seems to be due to positive national park effect as well as marine national park effect, the dynamic regional effect appears to have a negative effect on visitors. This paper also employs the positioning analysis on national parks using Boston Consulting Group matrix over the period 2011-2016. The BCG analysis shows that Odongdo and Dadohaehaesang succeed in rising to a star area, Byeonsanbando ranges over a cash cow to a child, but Taeanhaean degrades from a child to a dog area.

논문 : 국립공원 탐방로 내 위험 급경사지 유지관리 방안 연구

김홍균 ( Hong Gyun Kim ),김태호 ( Tae Ho Kim ),김재학 ( Jae Hak Kim ),곽재환 ( Jae Hwan Kwak ),박성욱 ( Sung Wook Park ),최수원 ( Soo Won Choi ),송영갑 ( Young Karb Song ) 대한지질공학회 2016 지질공학 Vol.26 No.1

국립공원은 주로 산악 지형 내 조성되어 있으므로 산사태에 의한 피해 가능성을 항상 내포하고 있다. 본 연구는 산사태 연구의 일환으로 국립공원 탐방로 내 위험 급경사지를 체계적으로 유지관리하기 위한 방안을 마련하는데 목적이 있다. 국립공원에 특화된 자체 체크리스트를 제작하였으며, 전국 19개 국립공원 183개소 급경사지에 대한 실태조사가 이루어졌다. 실태조사의 최종 목적은 위험 급경사지에 대한 안정화 대책을 마련하는 것으로 현장조사와 안정해석의 과정을 거친다. 일부 급경사지에서는 위험성 판단을 위해 지상라이다 등 특수 장비를 이용한 조사를 시도하였다. 중장기 정비계획을 위한 투자우선순위 공식을 개발하였으며, 현장자료를 이용한 순위 및 위험등급을 산출하였다. 최종적으로 위험 급경사지의 향후 체계적인 관리를 위해 “국립공원 급경사지 유지관리시스템”을 개발하였으며, 모든 현장자료들은 시스템 내 DB화 되었다. National parks, which are located mainly in mountainous areas, are always at risk of damage by landslides. The goal of this study is to establish a method for systematically maintaining hazardous steep slopes along trails in national parks. We produced a checklist suitable for each of the 19 national parks nationwide and investigated 183 slopes. The aim of these investigations is to recommend appropriate slope-stability countermeasures, including field investigations and stability analysis. We made preliminary investigations at specific sites, evaluating the slope hazard using specialized equipment such as terrestrial LiDAR. An investment priority formula was developed, and ranking and hazardous grades were calculated as part of a long-term maintenance plan. Finally, to systematically manage dangerous slopes and to house all the field data within one system, we developed the “Slope Maintenance System in National Parks” based on web server that can show various information for slopes.

대전시 도시숲의 식생 및 토양특성에 관한 연구

김동일 ( Dong Il Kim ),박관수 ( Gwan Soo Park ),김길남 ( Gil Nam Kim ),김현숙 ( Hyoun Sook Kim ),이항구 ( Hang Goo Lee ),박범환 ( Beom Hwan Park ),이상진 ( Sang Jin Lee ),강길남 ( Kil Nam Kang ) 한국환경복원기술학회(구 한국환경복원녹화기술학회) 2011 한국환경복원기술학회지 Vol.14 No.2

This study was conducted to suggest appropriate methods for management of urban forest after investigating the present condition and problems of urban forests by analyzing vegetation and soil properties in urban forests in the Daejeon. On the basis of our research, Pinus rigida dominate Gyejoksan and Bomunsan. Pinus densiflora dominate Wolpyeong park and Quercus acutissima dominate Namsun park. On the basis of our result of analysis of soil chemical properties, all investigated areas have low pHs, available phosphates and exchangeable cations. They indicate that the soil of those areas have been acidifying progressively. Soil hardness measurements were conducted to know the conditions of trampled soils and the results of them show that soil hardness in Namsun park was higher than the others. This indicates that human interference affect the health of the urban forest.

국내분리 Aujeszky's disease virus의 실험적 감염 자돈에 대한 바이러스학적 연구 : Pathogenecity, excretion, distribution and immunogenicity of virus

박정우,전무형,안수환 충남대학교부설 생명공학연구소 1992 생물공학연구지 Vol.2 No.-

To investigate the etiology, pathogenicity and virological properties of NYJ-1-87 strain of Aujeszky's disease virus (ADV) that was isolated from the diseased piglet in Korea, the virus at 10^(6.0)TCID_50/0.1㎖ was inoculated intranasally and subcutaneously into 30 to 35 days-old piglets. Results obtained through the experiments were summarized as follows. 1. Ten of the infected piglets were clinically observed for 15 days. On the 2nd day post-inoculation(pi), the signs of pyrexia, anorexia and convulsion were noted. On the 4th 7th days pi, nervous signs of incoordination and intermittent spasm were shown in the most of piglets, and one out of 5 piglets infected intranasally was died with severe nervous signs at the 7th day pi. The signs became relieved on the 8th day pi and all of remainder were completely recovered on the 13th to 14th days pi. 2. In hematological study, prominent decrease in the number of total leukocyte and lymphocyte was shown in the ADV-infected piglets on the 6th day pi. On the 8th day pi, the cell numbers were slightly increased and returned to normal level on the 10th day pi. 3. Viral excretion of the ADV-inoculated piglets was examined by swabbing of nasal and oral cavities, and rectal feces. During the periods of the 3rd to11th days pi, the virus was excreted intermittently from nasal and oral cavities, and rectal feces. The nasal excretions were shown the highest virus concentration of 10^(5.2)TCID_50/0.1㎖ at the 5th day pi. 4. Recovery of the inoculated virus from various organs of the piglets that were died or experimentally slaughtered was attempted, and the virus was isolated from the tissues of brain and tonsil by the cultured cell-inoculation method. The highest recovery rate was noted in the tonsil. By indirect immunofluorescence antibody assay using ADV-monoclonal antibody, the viral antigens were detected in tissues of spleen and liver as brain and tonsil on the 7th to 9th days pi. The virus was not isolated from blood and the tissues of lung and kidney throughout the experiments. 5. Titers of virus neutralizing antibody in the piglets experimentally infected with ADV became increased after the 6th to 9th days pi in both of intranasal and subcutaneous inoculation showing the highest titers of 64 to 128 on the 29th day pi. When the antibody levels were measured by radial immunodiffusion enzyme assay, the reactive diameter was enlarged to be positive after the 4th to 6th days pi in both of intranasal and subcutaneous inoculation showing the largest diameter of 13 to 14mm on the 29th day pi.

단기배양을 통한 말초혈액 CD34 양성세포의 체외증폭

박상준,김철희,배광봉,김현수,김종숙,윤환중,조덕연,김삼용 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.1

Background: It is suggested that clinical practice in the areas of bone marrow transplantation and gene therapy might rely on the ex vivo expansion of hematopoietic stem/progenitor cells. However, the condition for ex vivo expansion of hematopoietic progenitor cells is not well established. The authors pursued a series of experiments to define the proper conditions for the expansion of hematopoietic cells in the short-term liquid suspension culture of mobilized peripheral blood CD34+ cells. Methods: 1.0ml cultures were initiated with 9×10^3 PB CD34+ cells, which were isolated from PB mononuclear cells (MNCs) by high-gradient cell sorting, in 12 well plates with the various combinations of hematopoietic growth factors(HGF). The following recombinant human HGFs were used: stem cell factor(SCF) 100ng/ml, granulocyte colony-stimulating factor(G-CSF) 100ng/ml, GM-CSF(granulocyte, macrophage colony-stimulating factor) 100ng/ml, interleukin-1 beta(IL-1B) 1ng/ml, interleukin-3(IL-3) 20ng/ml, interleukin-6 (IL-6) 100ng/ml. At the end of culture, colony-forming cells were evaluated by semisolid clonogenic assay. Results: 1) Using the high-gradient magnetic sorting system, CD34^+ cells were isolated with a yield of 40 3% 2) In 7 day culture of PB CD34^+ cells(9×10^3 cells), nucleated cells expanded mean 10×10^3(range, 9 to 20×10^3) with the addition of SCF alone, 35×10^3(range, 10 to 60×10^3) with SCF plus G-CSF plus GM-CSF, and 130×10^3(range, 40 to 300×10^3) with the combination of SCF, G-CSF, IL-1, IL-3, IL-6, GM-CSF. In 14 day culture, nucleated cells expanded 10×10^3 to 1,860×10^3 with combination of human hematopoietic growth factors. 3) In 10 day culture without medium change of PB CD34^+ cells, CFU-GM numbers expanded 16. 5 fold(range, 7 to 59 fold) with the addition of SCF plus G-CSF plus Il-1 plus IL-3, 31.3 fold(range, 20.5 to 101.1 fold) with the combination of SCF, G-CSF, IL-1, IL-3, GM-CSF. In 14 day culture with or without medium change of PB CD34^+ cells was inferior to 10 day culture for CFU-GM expansion. 4) There was no significant difference for CFU-GM expansion between five growth factors(SCF,G-CSF,IL-1,IL-3,GM-CSF) and six growth factors(five growth factors plus IL-6). Conclusion: The authors could confirm that short-term suspension culture of peripheral blood CD34+ cells could expand hematopoietic progenitor cells. Ten-day culture with medium change of CD34+ cells with the addition of five growth factors, i.e. SCF, G-CSF, IL-1B, IL-3, and GM-CSF, might be the most efficient in this system.

산성전해수 제조용 RuO₂/Ti와 IrO₂/Ti 전극에 대한 전해방법으로 첨가된 MnO₂의 첨가시 전기화학적 특성

최종환, 박수길 충북대학교 건설기술연구소 2009 충북대학교 건설기술연구소 논문집 Vol.28 No.2

-본문참고-

冷却 條件 變化에 따른 저밀도 폴리에틸렌의 絶緣 破壤 特性에 관한 硏究

박정수,이수원,황종국,손인환 철도전문대학교 1993 論文集 Vol.10 No.1

In order to change the crystallinity of low density polyethylene film, the sample cooling with air, ice and liquid nitrogen was studied for the temperature dependence of dielectric breakdown strength at the temperature range of l0℃∼120℃ As a result, breakdown strength was influenced that increase of crystallenety was due to the decrease of dielectric loss under the glass transition temperature and to the decrease of effect for space charge with Micro Brown Ion motion over the glass transition temperature. In accordance with the rise of surrounding temperature, AC breakdown strength was less dependent of change of crystallinity but the DC and Impulse breakdown strength was highly dependent of et.

金屬薄膜導波管을 利用한 I/O Mode Filter

박한규,오한솔,김명수 연세대학교 산업기술연구소 1981 논문집 Vol.13 No.2

代表的인 3종류의 金屬薄膜導波管의 TEo-Mode와 TMo-Mode의 특성을 Computer Simulation에 의하여 비교 고찰하였다. 非對稱型平板薄膜導波管으로된 금속박막도파관은 TEo-Mode Filtering이 不可能하였다. 對稱型平板薄膜導波管으로된 금속박막도파관에서는 Film층과 그 외의 층과 굴절율 차이가 0.02 이하가 되면 TEo-Mode Filtering이 가능하며 TMo-Mode 의 損失이 10dB/cm이하가 되어 Mode Filter로서 使用可能함이 밝혀졌다. The typical three kinds of the Metal-Clad waveguides have been chosen, and the characteristics of TEo-Mode and TMo-Mode in them are investigated and compared with each other by computer simulation. TEo-Mode filtering has been impossible in the Metal-Clad waveguide made of the symmetric thin film slab waveguide. In the Metal-Clad waveguide made of the symmetric thin film slab waveguide, if the refraction index difference between the film layer and the other layers is below 0.02, it has made TEo-Mode filtering possible and has had TMo-Mode loss below 10dB/㎝. Therefore it has been improved that it can be used practically as a Mode Filter.

Pocine Adenovirus-3의 E1B Region의 鹽基序列 分析

朴鍾賢,宋載永,李重馥,玄芳勳,安東濬,車相昊,裵用泰,姜永源,Reddy, P S,全茂炯,安壽煥 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

돼지 아데노바이러스(PAV-3). 6618주의 EIB region이 包含되어 있는 map unit 4.0에서 9.7까지의 유전자에 대한 1,984 bp의 염기서열을 決定하였으며, 이 結果를 알려진 여러 아데노바이러스 유전자와 비교하여 다음과 같은 결과를 얻었다. 1. PAV-3의 EIB유전자는 10개의 ORF로 구성되어 있으며, 그 중 아데노바이러스의 단백질과 유사성이 있는 것은 ORF1, ORF2 및 ORF3이었다. ORF1은 Ad41의 19kd 와 BAV-2에서의 EIB ORF2에서의 아미노산의 一致率은 각각 32%와 31%이었다. 2. ORF2는 Ad2 55kd protein과 tupaia adenovirus 44kd protein가 각각 34%로 아미노산 一致率이 가장 높았으며, Ad41의 52kd protein. BAV-3의 EIB ORF3에서도 33%의 一致率을 보였다. 3. ORF1은 61-666 uncleotide (606 bp), ORF 2에서는 429-1,850 uncleotide (1,422 bp)의 부위로 각각 202, 474 a.a로 構成되었으며, 예상되는 분자량은 20 kd와 52 kd이었다. 4. ORF3는 hexon-associated pIX유전자로 추정되며 내부에 1개의 polyadenylation signal(ATAAA)이 1938-1942 uncleotide에 위치하였으며, 이 부위는 TATA box (1937-1942 uncleotide)와 중복되어 존재하였다. Porcine adenovirus type 3 (PAV-3) does not cause severe infection in pigs. Adenovirus has been suggestive of live vaccine vector carrying foreign gene. One of insertion regions is delayed early (EIB) region. However, EIB region of PAV-3 has not been molecularly characterized to date. Nucleotide sequence of EIB of PAV-3 was determined. The EIB region was composed of 1,984 bp and located between 4.0 and 9.7 map units. Three potential open reading frames(ORFs) with low level of homology to other adenoviruses and a polyadenylation signal were identified in the rightward direction of genome. The nucleotide and the predicted amino acid sequences of EIB were compared to those of human and animal adenoviruses. One of the three potential ORFs. ORF1 encoded a polypeptide homologous to bovine adenovirus type 2(BAV-2) ORF2 and human adenovirus type 41(Ad41) 19 kd protein. ORF2 encoded a polypeptide homologous to human adenovirus type 2(Ad2) 55 kd protein, bovine adenovirus type 3(BAV-3) ORF3 and porcine adenovirus type 4(PAV-4) ORF2. The predicted protein of ORF1 had homology to those of Ad41 and BAV-2 with 32 and 31% respectively, whereas the deduced protein of ORF2 had homology to those of Ad2. BAV-3 and PAV-4 with 34, 33 and 29%, respectively.