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李相和,李俊熹 동아대학교 환경문제연구소 1984 硏究報告 Vol.7 No.2
T₄ and T_(6) treated test alloy were investigated by the D. S. C. analysis. Low temperature exothermic reaction which caused by dissolution of G. P. Zone was formed during the aging at room temperature, and has relationship to the preformed precipitates, by which classical precipitation path can be changed to the modified precipitation path. In the D. S. C. curve, the region of low temperature endothermic reaction is due to the dissolution of G. P. Zones, and the region of high temperature endothermic reaction is associated with the disslution of equilibrium phase η, and the formation of η', η, and the growth of η appear at the middle region of temperature. Kinetics which related to the precipitation process of high strength Al alloy can be investigated with the aid of D. S. C. analysis more easily. The relative stability of precipitate in matrix grows down in the sequence of η, η' and G. P. Zone, and is mainly depended upon the activated entropy.
Xiong, Shang-Ling,Lim, Gyu Tae,Yin, Shang-Jun,Lee, Jinhyuk,Si, Yue-Xiu,Yang, Jun-Mo,Park, Yong-Doo,Qian, Guo-Ying Elsevier 2019 INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Vol.121 No.-
<P><B>Abstract</B></P> <P>Pyrogallol is naturally found in aquatic plant and has been proposed as a substrate of tyrosinase. In this study, we evaluated the dual effect of pyrogallol on tyrosinase as an inhibitor in the presence of L‑DOPA simultaneously via integrating methods of enzyme kinetics and computational molecular dynamics (MD) simulations. Pyrogallol was found to be a reversible inhibitor of tyrosinase in the presence of L‑DOPA and its induced mechanism was the parabolic non-competitive inhibition type (<I>IC</I> <SUB>50</SUB> = 0.772 ± 0.003 mM and <I>K</I> <SUB>i</SUB> = 0.529 ± 0.022 mM). Kinetic measurements by real-time interval assay showed that pyrogallol induced rapid inactivation process composing with slight activations at the low dose. Spectrofluorimetry studies showed that pyrogallol mainly induced regional changes in the active site of tyrosinase accompanying with hydrophobic disruption at high dose. The computational MD simulations further revealed that pyrogallol could interact with several residues near the tyrosinase active site pocket such as HIS61, HIS85, HIS259, ASN260, HIS263, VAL283, and ALA296. Our study provides insight into the mechanism by which hydroxyl group composing pyrogallol inhibit tyrosinase and pyrogallol is a potential natural anti-pigmentation agent.</P>
Yin, Shang-Jun,Cho, Ick-Hyun,Yang, Hee Seung,Park, Yong-Doo,Yang, Jun-Mo Elsevier 2018 International journal of biological macromolecules Vol.106 No.-
<P><B>Abstract</B></P> <P>Serum proteomics has been applied for the discovery and analysis of biomarkers related to human disease. Serum is an optimal source to identify proteins derived from diseased-tissue compartments. We recently established an integrative method to analyze highly basic proteins that remain unresolved by the general 2D-PAGE method. In this follow-up study, we successfully detected several disease-associated proteins from sera samples obtained from patients with atopic dermatitis (AD). After proteomic analyses, target proteins were validated from AD patient-derived sera using ELISA or Western blotting methods We detected zinc finger CCHC domain containing 10 (ZCCHC10), peptidoglycan recognition protein L (PGRP-L), kininogen, α-1-antitrypsin, and hornerin proteins that are dysregulated in AD patient sera samples, which suggest effective approaches to methodologically analyze the serum proteome. Thus, the integrated proteomic method approach described here could be applicable for the detection of proteins associated with other human diseases. Our present study provides new insights into optimized serum proteomic techniques to understand systemic events of AD.</P>
Yin, Shang-Jun,Zhang, Linmeng,Zhang, Lili,Wan, Jiaxin,Song, Wei,Jiang, Xiamin,Park, Yong-Doo,Si, Yue-Xiu Elsevier 2018 INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Vol.113 No.-
<P><B>Abstract</B></P> <P>The pharaoh cuttlefish <I>Sepia pharaonis</I> is particularly sensitive to environmental changes in its breeding environment. The breeding of <I>S</I>. <I>pharaonis</I> larvae was carried out in different salinities for 48h, and the changes in survival rate, histological structure, energy metabolism, and anti-oxidative stress parameters were investigated and correlated with arginine kinase (AK) expression changes in muscle and liver tissues. The suitable salinity for larvae cultivation ranged from 24 to 30‰, and the survival rate showed a significant decline at 21‰ salinity. Histological observations of muscle and liver showed that changes in salinity and osmotic pressure had an adverse effect on tissue structure. Measurements of glycogen and lactic acid levels suggested that <I>S</I>. <I>pharaonis</I> could dynamically adjust energy metabolism to provide additional energy under unsuitable salinity. The protein levels and enzyme activities of AK in muscle significantly increased at 21‰ salinity. The results were consistent with prompt replenishment of phosphoarginine stores during salinity stress to maintain a dynamic ATP balance, suggesting that AK plays an important role in the regulation of energy metabolism. This study provides insight into metabolic changes during salinity stress and sheds light on the functional role of AK in <I>S</I>. <I>pharaonis</I>.</P>
The effect of thiobarbituric acid on tyrosinase: inhibition kinetics and computational simulation.
Yin, Shang-Jun,Si, Yue-Xiu,Wang, Zhi-Jiang,Wang, Su-Fang,Oh, Sangho,Lee, Sanghyuk,Sim, Seon-Mi,Yang, Jun-Mo,Qian, Guo-Ying,Lee, Jinhyuk,Park, Yong-Doo Adenine Press 2011 Journal of biomolecular structure & dynamics Vol.29 No.3
<P>Tyrosinase plays various roles in organisms and much research has focused on the regulation of tyrosinase activity. We studied the inhibitory effect of thiobarbituric acid (TBA) on tyrosinase. Our kinetic study showed that TBA inhibited tyrosinase in a reversible noncompetitive manner (K(i) 5 14.0 ± 8.5 mM and IC?????? 5 8.0 ± 1.0 mM). Intrinsic and ANS-binding fluorescences studies were also performed to gain more information regarding the binding mechanism. The results showed that no tertiary structural changes were obviously observed. For further insight, we predicted the 3D structure of tyrosinase and simulated the docking between tyrosinase and TBA. The docking simulation was successful with significant scores (binding energy for AutoDock4: -5.52 kcal/mol) and suggested that TBA was located in the active site. The 11 ns molecular dynamics simulation convinced that the four HIS residues (residue numbers: 57, 90, 250, and 282) were commonly responsible for the interaction with TBA. Our results provide a new inhibition strategy that works using an antioxidant rather than targeting the copper ions within the tyrosinase active site.</P>
Yin, Shang-Jun,Si, Yue-Xiu,Chen, Yong-Fu,Qian, Guo-Ying,L?, Zhi-Rong,Oh, Sangho,Lee, Jinhyuk,Lee, Sanghyuk,Yang, Jun-Mo,Lee, Dong-Youn,Park, Yong-Doo Kluwer Academic/Plenum 2011 The Protein Journal Vol.30 No.4
<P>Tyrosinase inhibition studies are needed due to the agricultural and medicinal applications. For probing effective inhibitors of tyrosinase, a combination of computational prediction and enzymatic assay via kinetics were important. We predicted the 3D structure of tyrosinase from Agaricus bisporus, used a docking algorithm to simulate binding between tyrosinase and terephthalic acid (TPA) and studied the reversible inhibition of tyrosinase by TPA. Simulation was successful (binding energies for Autodock4 = -1.54 and Fred2.0 = -3.19 kcal/mol), suggesting that TPA interacts with histidine residues that are known to bind with copper ions at the active site. TPA inhibited tyrosinase in a mixed-type manner with a K ( i ) = 11.01 2.12 mM. Measurements of intrinsic and ANS-binding fluorescences showed that TPA induced no changes in tertiary structure. The present study suggested that the strategy of predicting tyrosinase inhibition based on hydroxyl groups and orientation may prove useful for screening of potential tyrosinase inhibitors.</P>
Why Worry about Earthquake in Regins of Moderate Seismicity : A Case-Study of Hong Kong
Kuang Jun Shang 한국지진공학회 1998 한국지진공학회논문집 Vol.2 No.3
This paper reports a part of research work on earthquake resistance consideration in regions of moderate seismicity, which is being carried out in the Department of Civil Engineering, Hong Kong University of Science & Technology. The possible seismic hazard in Hong Kong, which is located in a region of moderate seismicity, is described. A case study is presented to compare the wind and earthquake effects on Hong Kong buildings and to assess whether seismic analysis and desing is necessary for building structure. Potential problems of reinforced concrete buildings under earthquake effects in regions of moderate seismicity are discussed.
전상호(Jun Shang-Ho),이철훈(Lee Cheol-Hoon) 한국콘텐츠학회 2007 한국콘텐츠학회 종합학술대회 논문집 Vol.5 No.1
최근 임베디드 디바이스에 여러 가지 장점을 제공하는 자바 기술은 필수적인 요소가 되었다. 자바기술을 임베디드 디바이스에 적용하기 위해서는 자바 가상 머신의 코어 클래스(Core Classes)와 코어 클래스의 기능을 확장시켜주는 프로파일(profile)이 필요하다. 본 논문에서는 임베디드 자바를 위한 프로파일을 설계 및 구현하였다. 이 프로파일은 SUN Microsystems사의 MIDP(Mobile Information Device Profile)을 기반으로 하였으며, MIDP는 그래픽, 유저 인터페이스와 네트워크 기능을 명세하고 있다. 본 논문에서 프로파일은 UbiFOS™의 그래픽과 네트워크를 사용하는 네이티브(native) 함수를 정의하여 구현하였다. Recently, java technology that offers various advantages to the embedded device became essential element. To apply java technology to the embedded device needs the core-class of Java Virtual Machine and the profile that extends core-class. This paper deals with the design and implementation of the profile for the embedded java. The profile implemented is based on the MIDP(Mobile Information Device Profile) of the SUN microsystems. MIDP specifies the function of a graphic, user interface and network. In this paper, profile is implemented by definition of its native function using a graphic, network of the UbiFOS™.