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      • KCI등재

        왜 그들은 ‘술방’을 보는가?: 소셜미디어 음주 일인방송 시청동기와 콘텐츠 태도에 대한 탐색적 연구

        강승미(Seungmi Kang),유주연(Joo-Yeon Ryu),유승철(Seung-Chul Yoo) 강원대학교 사회과학연구원 2021 사회과학연구 Vol.60 No.2

        본 연구는 이용과 충족 이론을 바탕으로 술방 시청동기에 대한 탐색적 분석과 시청동기가 술방 콘텐츠 태도에 미치는 영향에 대한 효과 분석을 실시했다. 술방 시청동기에 대한 탐색적 분석 결과, 정보탐색, 음주 생동감, 시간때 우기, 사회적 상호작용, 대리만족, 재미의 여섯 가지 술방 시청동기를 도출했다. 또한, 성별 및 세대에 따라 시청동기에 차이가 있는지를 분석한 결과, 성별에 따른 차이는 없었으나 세대에 따른 시청동기의 차이는 유의했다. 정보탐색 시청동기는 30대와 40대와 비해 20대에서 더 높게 나타났으며, 음주 생동감 시청동기도 40대에 비해 20대에서 더 높게 나타났다. 시간때우기 시청동기는 50대에 비해 20대, 30대, 40대에서 더 높게 나타났다. 다음으로, 여섯 가지 술방 시청동기가 술방 콘텐츠 태도에 미치는 영향에 대한 효과를 검증한 결과, 술방 시청동기 6개 요인 중 시간때우기, 대리만족 동기를 제외한 나머지 4개 술방 시청동기(정보탐색, 음주 생동감, 사회적 상호작용, 재미)가 술방 콘텐츠 태도에 정(+)의 영향을 미치는 것으로 나타났다. 본 연구는 술방 시청동기를 처음으로 분석했으며, 술방 시청동기 중 ‘음주 생동감’ 동기는 기존 먹방 연구에서 발견하지 못한 술방만의 차별적 동기임을 밝혀냈다는 점에서 초기 연구로서 학술적 함의가 크다. 또한, 술방 콘텐츠 제작 실무와 식품 소셜 미디어 커뮤니케이션 전략에 함의를 준다는 점에서 실용적 가치가 크다. Based on the theory of Use and Gratification, this study conducted an exploratory factor analysis on Sulbang watching motivation and a research of Sulbang watching effect. Sulbang is defined as a drinking broadcast. The findings identified six factors: information seeking, drinking liveliness, time-passing, social interaction, vicarious satisfaction, and entertainment. Also, there was no significant difference in watching motivation by gender. However, three factors, information seeking, drinking liveliness, and time-passing, had a significant difference in watching motivation by generation. In information-seeking motivation, the mean value of 20s respondents was higher than those of 30s and 40s. In drinking liveliness motivation, the mean value of 20s respondents was higher than those of 40s. The mean value of the 50s was lower than those of 20s, 30s, and 40s in time-passing motivation. Also, this study examined whether there is a difference in content attitude depending on the Sulbang watching motivation. The results showed four Sulbang motivations (information seeking, drinking liveliness, social interaction, and entertainment) had a significantly positive effect on the Sulbang content attitude. Research implications are that it presents the first step toward understanding Sulbang watching motivation in an integrated way. Overall, this paper contributes practitioners to build global strategies of Korean food media and discuss youth protection regulations.

      • KCI등재

        아이스플랜트(Mesembryanthemum crystallinum)의 생화학적 성분 및 생리활성 효과

        강승미(Seungmi Kang),김선정(Seonjeong Kim),하수현(Suhyun Ha),이창렬(Changryul Lee),남상해(Sanghae Nam) 한국식품영양과학회 2016 한국식품영양과학회지 Vol.45 No.12

        아이스플랜트(Mesembryanthemum crystallinum)의 일반성분과 cyclitol 화합물을 분석하였으며, 기능성 식품의 소재로 활용 가능성을 알아보기 위하여 추출물의 용매분획별 항산화 효과 및 항당뇨 활성 등을 검정하였다. 무기질 함량은 K와 Na가 각각 1,213.33±2.52와 545.53±12.01 mg/100 g으로 가장 많았고, S, Ca, P, Mg의 순이었다. Cyclitol 화합물의 함량은 D-pinitol이 4.04±0.08 mg/g으로 가장 많았으며, chiro-inositol과 myo-inositol은 각각 2.82±0.01과 0.25±0.01 mg/g으로 비교적 적게 함유되어 있었다. 용매분획별 총페놀 함량은 chloroform과 ethyl acetate 분획에서 각각 35.80±1.33과 23.70±0.62 mg GAE/g으로 많았다. DPPH, ABTS, FRAP 및 lipid/MA assay에 의한 항산화 활성은 공통적으로 chloroform 분획에서 가장 높은 활성을 나타내었으며, ethyl acetate 분획에서 비교적 높은 활성을 나타내었다. 항산화 활성은 용매분획별 총페놀 함량과 비례적이었다. 한편 항당뇨 효과는 α-glucosidase 저해활성에서 모든 용매분획에서 50% 이상의 활성을 나타내었으며, ethyl acetate, butanol 및 chloroform 분획에서 각각 90.33±0.40, 87.98±0.16 및 86.38±0.51%의 높은 활성을 나타내었다. α-Amylase 저해 활성은 25.63±1.45~60.34±2.67%로서 α-glucosidase 저해 활성보다는 낮았으나, 용매분획별 저해 활성은 유사한 경향을 보였다. 이와 같은 연구 결과로 볼 때 아이스플랜트가 항산화 및 항당뇨 기능성 천연소재로 활용이 가능할 것으로 생각되었다. The general components and cyclitol compounds of ice plant (Mesembryanthemum crystallinum) were analyzed to examine the possibility of using extracts as functional food materials, the antioxidant effects and antidiabetic activities of the extracts by solvent fraction were tested. Among the mineral contents, contents of K and Na were the largest (1,213.33±2.52 and 545.53±12.01 mg/100 g, respectively), followed by S, Ca, P, and Mg in order of precedence. Among cyclitol compounds, content of D-pinitol was the largest (4.04±0.08 mg/g) while contents of chiro-inositol and myo-inositol were relatively small (2.82±0.01 and 0.25±0.01 mg/g, respectively). Among total phenol contents by solvent fraction, contents of chloroform and ethyl acetate fractions were large (35.80±1.33 and 23.70±0.62 mg GAE/g, respectively). Among antioxidant activity levels examined by DPPH, ABTS, FRAP, and lipid/MA assays, the chloroform fraction commonly showed the highest level of activity while the ethyl acetate fraction showed relatively high levels of activity. The antioxidant activity levels were proportional to total phenol contents by solvent fraction. As for antidiabetic effects, all solvent fractions showed at least 50% α-glucosidase inhibitory activity levels while the ethyl acetate, butanol, and chloroform fractions showed high levels activity of 90.33±0.40, 87.98±0.16, and 86.38±0.51%, respectively. The α-amylase inhibitory actively levels were in the range of 25.63±1.45∼60.34±2.67%, which was lower than the α-glucosidase inhibitory activity levels, but the inhibitory activity levels by solvent fraction were similar. Given the above study results, ice plant can be utilized as a natural material with antioxidative and antidiabetic functionality.

      • Covalent conjugation of mechanically stiff graphene oxide flakes to three-dimensional collagen scaffolds for osteogenic differentiation of human mesenchymal stem cells

        Kang, Seokyung,Park, Jong Bo,Lee, Tae-Jin,Ryu, Seungmi,Bhang, Suk Ho,La, Wan-Geun,Noh, Myung-Kyung,Hong, Byung Hee,Kim, Byung-Soo Elsevier 2015 Carbon Vol.83 No.-

        <P><B>Abstract</B></P> <P>Mesenchymal stem cells (MSCs) preferentially differentiate to osteogenic lineage when cultured on mechanically stiff substrates. However, collagen sponges, clinically approved scaffolds for bone regeneration, provide soft microenvironment to MSCs. Here, we demonstrate that the covalent conjugation of mechanically stiff graphene oxide (GO) flakes to three-dimensional (3D) collagen scaffolds improves the mechanical properties of the scaffolds and promotes the osteogenic differentiation of human MSCs (hMSCs) cultured on the scaffolds. The covalent conjugation of GO flakes to collagen scaffolds increased the scaffold stiffness by 3-fold and did not cause cytotoxicity. hMSCs cultured on the GO-collagen scaffolds demonstrated significantly enhanced osteogenic differentiation compared to cells cultured on non-modified collagen scaffolds. The enhanced osteogenic differentiation observed on the stiffer scaffolds was likely mediated by MSC mechanosensing because molecules that are involved in cell adhesion to stiff substrates were either up-regulated or activated. The 3D GO-collagen scaffolds could offer a powerful platform for stem cell research and orthopedic regenerative medicine.</P>

      • Ultraclean pre-concentration methods to control reliable water quality for polar snow and ice research

        Seungmi Lee,HeeJin Hwang,Hyejin Jung,Jung-Ho Kang 대한지질학회 2021 대한지질학회 학술대회 Vol.2021 No.10

        Polar snow and ice are essential research materials to reconstruct past climate change as those preserve the Earth"s atmospheric composition. To measure trace elements in snow and ice samples from the polar regions, we have to keep the clean protocol and maintain the water quality for low blank levels below sub picogram per gram or femtogram per gram level. We periodically check whether the water quality is maintained as well as the maintenance of a clean laboratory. In this study, we have tested the pre-concentration methods: heating evaporation method, graphite heating method, and vacuum freeze-drying method to confirm the water quality. Heating evaporation method concentrated sub-boiling water in a Teflon beaker at 100℃ using an aluminum heating block. The graphite heating method concentrated sub-boiling water in a Teflon bottle at 80°C using a graphite heating block. In vacuum freeze-drying method, sub-boiling water in a LDPE bottle was frozen at -60°C, and then freeze-dried at a pressure of 0.350 mbar. We have measured trace elements in the concentrated water samples using an inductively coupled plasma-sector field mass spectrometry (ICP-SFMS). This fundamental research method will establish a reliable and stable methodology to control the water quality for the analysis of trace elements in polar snow and ice.

      • Gold Nanoparticle/Graphene Oxide Hybrid Sheets Attached on Mesenchymal Stem Cells for Effective Photothermal Cancer Therapy

        Kang, Seokyung,Lee, Junghee,Ryu, Seungmi,Kwon, Yeji,Kim, Kyung-Hun,Jeong, Dae Hong,Paik, Seung R.,Kim, Byung-Soo American Chemical Society 2017 Chemistry of materials Vol.29 No.8

        <P>Cell-mediated nanoparticle delivery has been proposed for an effective cancer therapy. However, there are limitations in loading nanoparticles within cells as the internalized nanoparticles cause cytotoxicity and leak out of the cells via exocytosis. Here, we introduce hybrid sheets composed of gold nanoparticles (AuNPs) and graphene oxide (GO), which stably adhere to the cell surface and exhibit a remarkable photothermal effect. To form AuNP/GO sheets in which GO is sandwiched between two AuNP monolayers, AuNPs are coated with alpha-synuclein protein and subsequently adsorbed onto GO sheets. Attaching AuNP/GO sheets to the tumor-tropic mesenchymal stem cell (MSC) surface enhances the loading efficiency of AuNPs in MSCs by avoiding the cytotoxicity and exocytosis issues. Furthermore, the tight packing of AuNPs on microscaled GO sheets enhances the photothermal effect via strong plasmon coupling between AuNPs. The injection of AuNP/GO sheet-attached MSCs into tumor-bearing mice significantly improves the photothermal therapeutic efficacy by delivering larger amounts of AuNPs to the tumor and generating higher heat at the tumor region compared to injection of AuNP-internalized MSCs. The system of attaching AuNP/GO hybrid sheets to the tumor-tropic cell surface may be an effective platform for cancer therapy.</P>

      • Reversible Cell Layering for Heterogeneous Cell Assembly Mediated by Ionic Cross-Linking of Chitosan and a Functionalized Cell Surface Membrane

        Ryu, Seungmi,Kim, Hyunbum,Kang, Seokyung,Shin, Kwangsoo,Jung, Seon-Yeop,Heo, Jiwoong,Han, Jin,Yoon, Jeong-Kee,Lee, Ju-Ro,Hong, Jinkee,Ahn, Kyung Hyun,Hyeon, Taeghwan,Hwang, Nathaniel Suk-Yeon,Kim, Byu American Chemical Society 2017 Chemistry of materials Vol.29 No.12

        <P>Current heterogeneous cell assembly techniques in coculture systems rely on irreversible cell layering or a cell separation membrane. However, the techniques possess major drawbacks of inefficiency in direct interactions of the assembled cell layers and cell separation following coculture, which hamper characterization and therapeutic applications of the cells following coculture. Here, we develop a reversible cell layering platform for assembly of heterogeneous cells that allows both active direct cell cell interactions and facile cell separation. Anionic maleimide-chondroitin-sulfate is grafted onto the surface membrane of myogenic C2C12 cells and human mesenchymal stem cells (hMSCs) to modify the surface charge of the cells without cytotoxicity. A highly porous chitosan thin film is formed in situ interspacing between the heterogeneous cell layers via ionic cross-linking of cationic chitosan and anionic functionalized cells, forming compactly assembled double-layered cell constructs. The chitosan film enables layering of the cells, which allows active direct interactions between the cell layers, and facile delayering of the cells through simple treatment with mild shear stress. The developed platform promotes the myogenic commitment of hMSCs via direct contact with C2C12 cells, mimicking the interactions that trigger stem cell differentiation in vivo. Delivery of the myogenic committed cells to muscle injured animal models shows evident muscle regeneration.</P>

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