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Seunghyun Lee,Yerim Lee,Seunghwan Lee 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.10
The notorious lamiine species Asian Longhorn Beetle, Anoplophora glabripennis, is one of the major invasive pests of street trees, which is an endemic species of Korea and China. By far, the distributional range of Korean A. glabripennis population is restricted to only central to northeastern forest region of South Korea. In this study, we found unreported A. glabripennis populations in 4 different port cities and adjacent cities. These new populations are found in very much urbanic regions; Incheon, western part of Seoul, Busan and Ulsan. These populations can be distinctly differentiated with previously reported populations by massive damage on trees, unusual usage of host plant and spotted elytral pattern. To test their identity, we analyse the genetic patterns of the A. glabripennis populations sampled across South Korea based on three mitochondrial genes, Cytochrome Oxidase I – leucin tRNA – Cytochrome Oxidase II (COI-tRNALeu- COII). Additionally, we used available sequences of COI - tRNALeu - COII of 104 Chinese and 15 Korean individuals from NCBI. Here we show that Korean population is distinctly divided into two major clades, namely native populations and non-native ones, with 0.7% ~ 1.2% of genetic divergence. Hence, the non-native ones can be divided into two subgroups, which we call Incheon-Seoul complex and Busan-Ulsan complex. We observed that these newly found groups, Incheon-Seoul complex and Busan-Ulsan complex share perfectly identical COI haplotype within each group. This study strongly suggests that newly found populations are introduced respectively and these populations might be derived from very small number of introduced founders.
Lee Seunghyun,Ko Gyeonghun,Lee Yeong-Don,Keun Lee Sun,Kim Min-Jung,Lee Minho,Kang Kyung-Don,Ming Bai,김동순,Lee Seunghwan 한국응용곤충학회 2024 Journal of Asia-Pacific Entomology Vol.27 No.2
The longhorned beetle genus Anoplophora contains highly destructive species in the field of invasive entomology. Recently, the establishment of the non-native member of the genus, Anoplophora horsfieldii (Hope, 1843), has been documented in South Korea. However, the degree of impact of A. horsfieldii in invaded area remains un certain due to the limited number of individuals surveyed, making it harder to classify them as invasive. This study combines data from both citizen scientists and naturalists to investigate the occurrence status and distri bution range of A. horsfieldii. In total, 1,156 individuals were collected, with 1,124 of them collected in 2023 alone. Citizen-contributed data accounted for 52.5%, enabling us to estimate the preliminary occurrence period and population peak. This research identifies A. horsfieldii as the third invasive species within the genus Ano plophora and underscores the importance of citizen science in early detection and ecological surveys of invasive species.
Stable isotope on the evaluation of water quality in the presence of WWTPs in rivers
Lee, Jaewoong,Park, Taejin,Kim, Min-seob,Kim, Jongmin,Lee, Seunghyun,Lee, Su Kyuong,Lee, Young Sun,Lee, Won-seok,Yu, Soonju,Rhew, Doughee Springer-Verlag 2016 Environmental science and pollution research inter Vol.23 No.18
<P>We investigated the distribution of nitrogen compounds in Han River as well as two tributaries of Tancheon and Jungrangcheon. Particularly, we observed the significant releases of NH4+-N from effluent of wastewater treatment plants (WWTPs) in two tributaries that has resulted in the increases of ammonium nitrogen (NH4+-N) in Han River as well as in Tancheon and Jungrangcheon. Due to the increases of NH4+-N in two tributaries, the larger distribution of delta N-15-NH4+ was observed than those of delta N-15-NO3- in downstream. We calculated the contribution rate of ammonium nitrogen and nitrate nitrogen from effluent to downstream according to the results of stable isotope. The contribution rates of delta N-15-NH4+ from effluent to downstream were significant that ranged between 53 and 100 % in Tancheon and between 27 and 100 % in Jungrangcheon. While the contribution of delta N-15-NO3- was not significant in Tancheon, it was occasionally observed in Jungrangcheon. These results demonstrated that WWTPs are the major sources of NH4+-N in two tributaries, which caused the distinguishable stable isotope of delta N-15-NH4+. Therefore, the stable isotope of delta N-15-NH4+ could be a useful parameter or tracer for the evaluation of NH4-N released from WWTPs in rivers.</P>
Seunghyun Lee,Seunghwan Lee 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.10
The Lamiine subgenus Pseudale Schwarzer, 1925 from Korean peninsula is reviewed. Along with the only recorded species Pterolophia (Pseudale) jiriensis Danilevsky, 1996, we found two additional new species based on morphological and genital difference. Descriptions, photographs of habitus and male genitalia of each species and a key to species of Korea are provided herein. Hence, genital variation of 4 different geographical populations of Pterolophia (Pseudale) jiriensis is discussed.
Lee, Seunghyun,Lee, Changkyu,Kim, Bomi,Thao, Le Quang,Lee, Eun Seong,Kim, Jong Oh,Oh, Kyung Taek,Choi, Han-Gon,Youn, Yu Seok Elsevier 2016 Colloids and Surfaces B Vol.147 No.-
<P><B>Abstract</B></P> <P>Albumin has been viewed as one of the most attractive biomacromolecules for making nanoparticulate systems due to its biocompatibility and chemical functionality. Thus far, albumin nanoparticles (NPs) are prepared by several limited methods, such as, desolvation, emulsification or high-pressure homogenization. In this article, we introduce a new albumin NPs prototype fabricated <I>via</I> a ‘host’ (β-cyclodextrin)-‘guest’ (adamantane) supramolecular association. These NPs (GC-CD/HSA-ADA NPs) consisted of β-cyclodextrin-modified glycol chitosan (GC-CD) and adamantane-conjugated human serum albumin (HSA-ADA) (GC-CD/HSA-ADA NPs) that were facilely prepared by a consequent dropwise mixing and sonication method. Doxorubicin-loaded GC-CD/HSA-ADA NPs exhibited an appropriate particle size (∼260nm), good physicochemical stability (∼48h), significant HCT116 cell cytotoxicity (IC<SUB>50</SUB>: 0.32μg/ml) and cell internalization. Furthermore, GC-CD/HSA-ADA NPs showed excellent tumor targetability probably due to gp60-mediated transcytosis mechanism because it was markedly accumulated in the tumor site of a HCT116 cell-xenograft mouse. Based on these results, these albumin NPs will be promising for a new NP platform that can be applied for cancer therapy or imaging.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We developed a new prototype of self-assembled albumin nanoparticles. </LI> <LI> The nanoparticles consisted of cyclodextrin-glycol chitosan and adamantane-albumin. </LI> <LI> The nanoparticles were formed <I>via</I> supramolecular cyclodextrin-adamantane association. </LI> <LI> The nanoparticles exhibited remarkable targetability in the HCT116 xenograft tumors. </LI> <LI> These new albumin nanoparticles would be potential as a theranostic carrier. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Lee, Seunghyun,Lee, Youn-Sun,Choi, Kyeong-Mi,Yoo, Kwang-Sik,Sin, Dong-Mi,Kim, Wonkyun,Lee, Yong-Moon,Hong, Jin-Tae,Yun, Yeo-Pyo,Yoo, Hwan-Soo Hindawi Publishing Corporation 2012 Evidence-based Complementary and Alternative Medic Vol.2012 No.-
<P>Sphingomyelin is the most abundant sphingolipid in mammalian cells and is mostly present in the plasma membrane. A new analytical method using high-performance liquid chromatography (HPLC) was developed to quantify sphingomyelin in mouse plasma and tissues, 3T3-L1 cells, rat aortic smooth muscle cells, and HT-29 cells. Sphingomyelin and dihydrosphingomyelin, an internal standard, were separated by high-performance thin-layer chromatography and simultaneously hydrolyzed with sphingolipid ceramide <I>N</I>-deacylase and sphingomyelinase to release sphingosine and dihydrosphingosine, respectively. Sphingomyelin content was measured by HPLC following <I>o</I>-phthalaldehyde derivatization. Sphingomyelin concentrations in 3T3-L1 cells, rat aortic smooth muscle cells, and HT-29 cells were 60.10 ± 0.24, 62.69 ± 0.08, and 58.38 ± 0.37 pmol/<I><I>μ</I></I>g protein, respectively, whereas those in brain, kidney, and liver of ICR mice were 55.60 ± 0.43, 43.75 ± 0.21, and 22.26 ± 0.14 pmol/<I><I>μ</I></I>g protein. The sphingomyelin concentration in mouse plasma was 407.40 ± 0.31 <I><I>μ</I></I>M. The limits of detection and quantification for sphingomyelin were 5 and 20 pmol, respectively, in the HPLC analysis with fluorescence detection. This sensitivity was sufficient for analyzing sphingomyelin in biological samples. In conclusion, this analytical method is a sensitive and specific technique for quantifying sphingomyelin and was successfully applied to diverse biological samples with excellent reproducibility.</P>
A New Record of the Subfamily Prometopinae (Coleoptera: Cucujoidea: Nitidulidae) from Korea
Seunghyun Lee,Seunghwan Lee 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.10
The subfamily Prometopinae Böving and Craighead, 1931 (Coleoptera: Cucujoidea: Nitidulidae) is newly recorded from Korea. We recognized two species in two different genera; Prometopia unidentata Hisamatsu, 1959, Parametopia x-rubrum Reitter, 1884. In this study, we provide re-description, photographs and a key to species based on Korean specimens.
Lee Seunghyun,Shim Dong Jae,Kim Doyoung,Cho Soo Buem,Baek Seung Hwan,Lee Edward Wolfgang,Lee Jung Whee 대한영상의학회 2024 Korean Journal of Radiology Vol.25 No.11
Objective: The aim of this study was to analyze the origins of prostatic arteries (PAs) in the Korean population and compare them with those reported in the literature. Materials and Methods: From April 2018 to February 2024, 108 male (mean age ± standard deviation: 71.6 ± 9.7 years) with lower urinary tract symptoms (n = 102) or refractory hematuria (n = 6) underwent prostatic artery embolization (PAE). Computed tomography and angiography images were retrospectively reviewed. The branching pattern of the internal iliac artery (IIA) was classified according to the Yamaki system. The origin of the PA was categorized using the de Assis definition, and the incidence of each type was recorded. A systematic literature review was conducted and the most common types of PA were investigated. Results: PAE was successfully implemented on 211 of the 216 pelvic sidewalls. PA cannulation failed in five sidewalls due to a steno-occlusive state. The most common IIA type was type A, in which the IIA was divided into the superior gluteal artery and gluteal–pudendal trunk (77%). Of 226 PAs analyzed, including 15 in 211 sidewalls exhibiting dual PAs, the most common PA origin was the internal pudendal artery (type IV, 35%), followed by the superior vesical (type I, 25%) and obturator (type III, 21%) arteries. Anterior division of IIA (type II) was less common (10%). Type V (uncommon origins) occurred in 8% of cases, including five distal internal pudendal arteries, four quadfurcations, three inferior gluteal arteries, three trifurcations, two medial femoral circumflex arteries, and two rectal arteries. Two of the five patients with surgically or endovascularly altered anatomy were successfully treated via PAs originating from the medial femoral circumflex arteries. Globally, type I is the most common PA type. Conclusion: In the Korean population, the most common IIA pattern and PA origin were types A and IV, respectively.