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월악산 잣나무 및 화백나무 형성층의 계절적 활동과 적산온도와의 관계
박준희, 강현정, 이해진, 서정욱 충북대학교 농업과학기술연구소 2022 農業科學硏究 Vol.38 No.2
The current study was conducted to investigate (1) the duration of cambial activity and (2) the effect of degree days on the initiation of cambial activity of the Pinus koraiensis (PK) and Chamaecy- paris picifera (CP) planted in Mt. Worak. In addition, the differences of the seasonal cambial activities according to the diameter were also investigated using C. picifera with a diameter of 30 cm (CPL) and 15 cm (CPS), which were planted in the same year. The cambium samples were weekly collected using a mini-borer (Ø 2 mm), so called Traphor, between April and October in 2017. The results obtained are as follow. The initiation of the cambial activity of CP (April 21-28) were slightly earlier than PK (April 21). The species with the longest cambial activity was CPS (147.2±12.1 days), followed by PK (141.6±9.8 days) and CPL (139±12.1 days). The degree days inducing the initiation of the cambial activ- ity for PK and CP were 180.5 and 180.5-242.8, respectively.
Hyeon Gyu Seo,Han Byeol Kim,Min Jueng Kang,Ji Young Yoon,Tae Hyun Kweon,Yun Soo Park,Jingu Kang,Jinwoo Jung,Eugene C. Yi,Tae Ho Lee,Jin Won Cho 한국당과학회 2018 한국당과학회 학술대회 Vol.2018 No.07
X -linked inhibitor of apoptosis (XIAP), an inhibitor of apoptotic cell death, has a RING domain and functions as an E3 ligase, catalysing the ubiquitination of various substrates such as apoptosis-inducing factor, TGF- [3-activated kinase 1, and MEK kinase 2. Here, we identified 0-linked N-acetylglucosamine ( 0-GlcNAc) transferase ( OGT) as a substrate of XIAP. We showed that OGT catalyses the 0-GlcNAc modification of XIAP at serine 406, and this modification regulates the E3 ligase activity of XIAP towards OGT. Substitution of XIAP Ser406 by alanine decreased its E3 ligase activity toward OGT but not toward other substrates. Stable overexpression of XIAP in HCT116 human colorectal carcinoma cells decreased OGT protein levels and inhibited cancer cell growth and invasion. These results suggest that XIAP requires 0-GlcNAcylation in the regulation of its E3 ligase activity toward OGT, which leads to OGT degradation and the inhibition of cancer cell growth.
Long-term Repeated Fed-batch Ethanol Fermentation in Aerated Condition
Seo, Hyeon-Beom,Yeon, Ji-Hyeon,Chung, Myung-Hoon,Kang, Do-Hyung,Lee, Hyeon-Yong,Jung, Kyung-Hwan 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.2
In this study, we attempted to assess the process stability of long-term fed-batch ethanol fermentation in the absence and presence of aeration (0.33 vvm). To examine the effect of aeration, a long-term repeated fed-batch operation was conducted for 396 h to mimic a long-term industrial bioethanol production process. In this long-term repeated fed-batch ethanol fermentation experiments, withdrawal-fill operation were conducted every 36 h for 10 repeat cycles. The whole operation was stably sustained in a quasi-steady state. The average maximal cell concentration and the average maximal ethanol production during operation were increased by 81.63 and 12.12%, respectively, when aeration was used. In addition, since aeration was carried out, the average ethanol yield slightly decreased by 4.03% and the average specific ethanol production rate decreased by 46.75% during operation. However, the average ethanol productivity increased by 17.53% when aeration was carried out. After 396 h of long-term repeated fed-batch ethanol fermentation, 1,908.9 g of ethanol was cumulatively produced when aeration was used, which was 12.47%, higher than when aeration was not used (1,697.2 g). Meanwhile, glycerol production was greatly decreased during long-term repeated fed-batch ethanol fermentation, in which the glycerol concentration in the culture broth decreased from about 34~15 g/L. Thus, we can conclude that cell growth was greatly improved by overcoming ethanol inhibition and glycerol production was remarkably decreased when aeration was carried out, although aeration in ethanol fermentation decreased the specific ethanol production rate and ethanol yield.
( Kang-seo Park ),( Yong Sang Hong ),( Junyoung Choi ),( Shinkyo Yoon ),( Jihoon Kang ),( Deokhoon Kim ),( Kang-pa Lee ),( Hyeon-su Im ),( Chang Hoon Lee ),( Seyoung Seo ),( Sang-we Kim ),( Dae Ho Lee 생화학분자생물학회(구 한국생화학분자생물학회) 2018 BMB Reports Vol.51 No.12
Human epidermal growth factor receptor 2 (HER2) inhibitors, such as trastuzumab and lapatinib are used to treat HER2-positive breast and gastric cancers. However, as with other targeted therapies, intrinsic or acquired resistance to HER2 inhibitors presents unresolved therapeutic problems for HER2-positive gastric cancer. The present study describes investigations with AUY922, a heat shock protein 90 (HSP90) inhibitor, in primary lapatinib-resistant (ESO26 and OE33) and lapatinib-sensitive gastric cancer cells (OE19, N87, and SNU-216) harboring HER2 amplification/over-expression. In order to investigate whether AUY922 could overcome intrinsic and acquired resistance to HER2 inhibitors in HER2-positive gastric cancer, we generated lapatinib-resistant gastric cancer cell lines (OE19/LR and N87/LR) by continuous exposure to lapatinib in vitro. We found that activation of HER2 and protein kinase B (AKT) were key factors in inducing intrinsic and acquired lapatinib-resistant gastric cancer cell lines, and that AUY922 effectively suppressed activation of both HER2 and AKT in acquired lapatinib-resistant gastric cancer cell lines. In conclusion, AUY922 showed a synergistic anti-cancer effect with lapatinib and sensitized gastric cancer cells with intrinsic resistance to lapatinib. Dual inhibition of the HSP90 and HER2 signaling pathways could represent a potent therapeutic strategy to treat HER2-positive gastric cancer with intrinsic and acquired resistance to lapatinib. [BMB Reports 2018; 51(12): 660-665]
A bright blue fluorescent dextran for two-photon <i>in vivo</i> imaging of blood vessels
Lee, Seo Hyeon,Choe, Young Ho,Kang, Rae Hyung,Kim, Yu Rim,Kim, Na Hee,Kang, Sangrim,Kim, Youngseo,Park, Sungnam,Hyun, Young-Min,Kim, Dokyoung Academic Press 2019 Bioorganic chemistry Vol.89 No.-
<P><B>Abstract</B></P> <P>Fluorescence-based <I>in vivo</I> imaging is one of the most important tools for monitoring of biological processes in cells and tissues of live animal models. Fluorescence imaging agents have also been used to monitor the microcirculation. Tracking microcirculation of the blood is vital to gain further insight into various vascular disease-related anomalies within the human body. As monitoring of vascular circulation is performed with visualization of both immune cells and pathogens, which are mainly labelled with red and green, the favorable color option for blood vessels could be blue. However, currently available blueish color-labeled agents for vascular monitoring is generally confronted with quick bleaching, because of its short excitation and emission wavelengths. Hereby, what we propose in this report is a newly generated bright blue fluorescent dextran, named HCD-70K that monitors the blood vessels using blue and inter-compatible typical fluorescent materials. DBCO-functionalized dextran-70K was fabricated with hydroxy-coumarin dye via metal-free bioorthogonal click chemistry, and generated HCD-70K, which can flow within the blood vessel and decipher the whole structure of the blood vessel successfully. The synthesis, spectroscopic analysis, and quantum chemical calculations were conducted. Using two-photon microscopy, efficient deep <I>in vivo</I> blood vessel imaging of a mouse model revealed exceptional bio-imaging capabilities of the HCD-70K and consequently it provided a promising opportunity for efficient vascular visualization in various research areas.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Two-photon excitable bright blue fluorescent dextran for blood vessels imaging. </LI> <LI> Stable blood flow identification, without risk of leakage. </LI> <LI> Multi-color two-photon <I>in vivo</I> imaging ability with both green-/red-emitting substrate. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>