Matrix Metalloproteinase-13 - A Potential Biomarker for Detection and Prognostic Assessment of Patients with Esophageal Squamous Cell Carcinoma

Sedighi, Maryam,Aledavood, Seyed Amir,Abbaszadegan, MR,Memar, Bahram,Montazer, Mehdi,Rajabian, Majid,Gholamin, Mehran Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.6

Background: Matric metalloproteinase (MMP) 13 gene expression is increased in esophageal squamous cell carcinomas (ESCCs) and associated with increasing tumor invasion, lymph node involvement and decreased survival rates. Levels of the circulating enzyme may be elevated and used as a marker of tumor progression. In this study, clinical application of MMP-13 serum levels was evaluated for early detection, prediction of prognosis and survival time of ESCC patients. Materials and Methods: Serum levels of MMP13 were determined by ELISA in 66 ESCC patients prior of any treatment and 54 healthy controls for comparison with clinicopathological data through statistical analysis with Man Whitney U and Log-Rank tests. In addition, clinical value of MMP13 levels for diagnosis was evaluated by receiver operating characteristic (ROC) test. Results: The serum level of MMP-13 in patients (>250 pg/ml) was significantly higher than in the control group (<100 pg/ml) (p value=0.004). Also the results showed a significant correlation between MMP-13 serum levels with tumor stage (p value = 0.003), depth of tumor invasion (p value=0.008), involvement of lymph nodes (p value = 0.011), tumor size (p value = 0.018) and survival time. While there were no significant correlation with grade and location of tumors. ROC analysis showed that MMP-13 level is an accurate diagnostic marker especially to differentiate pre-invasive/ invasive lesions from normal controls (sensitivity and specificity: 100%). Conclusions: These findings indicate a potential clinical significance of serum MMP13 measurement for early detection and prognostic assessment in ESCC patients.

Clinicopathologic Characteristics of Male Breast Cancer: A Report of 21 Cases in Radiotherapy Center of Hamedan, Iran

Sedighi, Abdolazim,Hamed, Ehsan Akbari,Mohammadian, Kamal,Behnood, Sepideh,Kalaghchi, Bita Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Background: Male breast cancer accounts for less than 1% of all cancer in men and only around 1% of all diagnosed breast cancer. Despite a significant raise in the last 25 years, it still remains a rare disease. Materials and Methods: We conducted a retrospective study from 2004-2011 with 21 male breast cancer patients. We aimed to analyze the epidemiologic data (age, personal and family history), tumor characteristics (size, histological type, location, TNM stage, receptors), surgery, adjuvant chemotherapy and radiation therapy, hormonal therapy and survival (relapse, follow up, death) who reffered to our center with breast cancer. Results: The median age was $49.2{\pm}14.2$ years (range 30-83 years). A family history of breast cancer was noted in four cases. The main clinical complaint was a retroareolar mass in 85.7%of patients (n=18). Histologically, 85.7% (n=18)were invasive ductal carcinoma and 4.7% (n=1) had ductal carcinoma in situ and 9.4% (n=2) had mixed histology including invasive medullary and ductal carcinoma. Hormonal therapy was delivered to 16 cases (76.1%) due to ER or PR positivity. During median follow up of 30 months (3-84 month), distant metastases were evident in 4 cases (19%). During the follow-up period, only one patient died due to metastatic disease. The mean time to recurrence detection was 30 months. Conclusions: The percentage of cases of male breast cancer is very low compared to breast cancer in females, explaining why very few investigations have been conducted in Iran. Limited coverage in the literature make gender-specific findings difficult so future research of this entity involving multi-institutional cooperation and longer follow up is essential to provide new insights about the biological and clinical factors of this rare cancer.

Variable gutter technique as a novel method to reduce waste material in closed die-forging process

M. Sedighi,M. Pourbashiri 대한기계학회 2014 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.28 No.12

Advanced forging simulation codes leads die designer toward more efficient methods in waste material reduction. In this paper, an approachhas been presented to reduce waste material by controlling material flow with variable gutter. Initially, an algorithm is offered fordecision making. A control criterion (δ) has been suggested in order to determine the new modified gutter thickness and width. A newformula has been offered to calculate the new dimensions of gutter width and thickness base on (δ) criterion in each step. To evaluate theeffectiveness of the proposed criterion and formula a set of Finite volume and finite element simulations has been done on different forgingparts. Then a “T shape” part has been chosen as a case study to investigate the effect of variable gutter width method and variablegutter thickness method separately. The FVM and FEM simulation results for variable thickness and width show a waste material reductionof 12% and 14%, respectively. Finally an experimental test has been setup to verify the simulation result of variable gutter thicknessmethod. The simulation and experimental results have a good agreement.

Ethanethiol Degradation by Ralstonia eutropha

Mahsa Sedighi,Farzaneh Vahabzadeh,Seyed Morteza Zamir,Abbas Naderifar 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.4

In the present study, a pure culture of Ralstonia eutropha was used to degrade gaseous ethanethiol. Ethane thiol at various initial concentrations ranging from 115 to 320 mg/m3 was degraded almost completely within 120 ~168 h, while at higher concentrations up to 452 mg/m3,removal efficiency declined. It was likely that ethanethiol was used as the source of energy by R. eutropha, since no clear increase in the biomass concentration was observed. Kinetic data of ethanethiol bidegradation could be fitted using the Monod model. The kinetic parameters were qm =0.23 (mg ethanethiol/g biomass/h), and Ks = 1.379 (mg/L). The mineralization pathway of ethanethiol through sulphate,as the detected product, and the energy production were discussed in some detail.

Kinetic Modeling of Cometabolic Degradation of Ethanethiol and Phenol by Ralstonia eutropha

Mahsa Sedighi,Farzaneh Vahabzadeh 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.2

Cometabolism, as a complex phenomenon inmicrobial world, is a special mechanism for transformationof many compounds of environmental and toxicologicalsignificance. Several models have been proposed to describethe cometabolic transformations of non-growth substratesin the absence or presence of growth substrates. In thisstudy, a model was proposed to simulate the degradationkinetics of phenol and ethanethiol (ET) by a pure culture ofRalstonia eutropha, including the effects of cell growth,endogenous cell decay, loss of transformation activity,competitive inhibition between growth and non-growthsubstrates, and self-inhibition of non-growth substrate. Themodel parameters were determined independently andwere then used for evaluating the applicability of the modelby comparing experimental data with model predictions. The model successfully predicted ET transformation andphenol utilization for a wide range of concentrations of ET(0 ~ 40 mg/L) and phenol (0 ~ 100 mg/L).

Catalytic performance of CeAPSO-34 molecular sieve with various cerium content for methanol conversion to olefin

Mehdi Sedighi,Mostafa Ghasemi,Alireza Jahangiri 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.4

A series of CeAPSO-34s with various cerium contents was synthesized and characterized by multiple techniques such as XRD, SEM, BET, 29Si MAS NMR, NH3-TPD and CO2-TPD. NH3-TPD spectra showed that a number of acid sites, especially those of strong acidity, is reduced with the increasing of Ce incorporation. Incorporation of metal ions gave rise to more silica-islands in the CeAPSO-34 framework. CO2-TPD showed that basic sites on the surface of modified samples are due to the presence of Ce-containing species incorporation into the framework of CeAPSO-34 molecular sieves. The performance of the catalysts was studied in methanol to olefin reactions at 425 oC under the atmospheric pressure. The results showed that the incorporation of cerium ions had great effects on the structure and acidity of the molecular sieves. All SAPO-34 and MeAPSO-34 molecular sieves were the very active and selective catalyst for light olefins production. Cerium incorporation improved the catalyst lifetime and favored the ethylene and propylene generation. However, an excess Ce content resulted in an inferior catalytic performance and stability. Therefore, there existed optimal cerium content for a specific SAPO-34.

Kinetic modeling formulation of the methanol to olefin process: Parameter estimation

Mehdi Sedighi,Hussein Bahrami,Jafar Towfighi 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.5

Detailed kinetic models at the elementary step level were developed for the methanol to olefins (MTO) process over SAPO-34 catalyst. Starting from believable mechanisms, forming primary products was modeled rigorously by the Hougen–Watson formalism. Discrimination of kinetic equations and calculation of the parameters of best fit were performed by solving the mass conservation equations of the main products of the kinetic scheme. For rate constants, preexponential factors and apparent activation energies were then calculated according to the Arrhenius equation. For thermodynamic constants, the difference between apparent activation energies of forward and reverse reaction was considered. The kinetic model fits well the experimental data, which is obtained in a fixed bed reactor. The results showed that rising space-time is favorable for olefin yields while an optimum temperature might produce the maximum olefin.

Static and dynamic pull-in instability of multi-walled carbon nanotube probes by He’s iteration perturbation method

Hamid M. Sedighi,Farhang Daneshmand 대한기계학회 2014 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.28 No.9

A continuum model is utilized to extract the nonlinear governing equation for Carbon nanotube (CNT) probes near graphite sheets. The van der Waals (vdW) intermolecular force and electrostatic actuation are included in the equation of motion. Static and dynamicpull-in behavior of the system is investigated in this paper. To this end, a new asymptotic procedure is presented to predict the pull-ininstability of electrically actuated CNTs by employing an analytic approach namely He's iteration perturbation method (IPM). The effectsof basic non-dimensional parameters such as initial amplitude, intermolecular force, geometrical parameter and actuation voltage on thepull-in instability as well as the fundamental frequency are studied. The obtained results from numerical simulations by employing threemode assumptions verify the strength of the analytical procedure. The qualitative analysis of the system dynamics shows that the equilibriumpoints of the autonomous system include stable center points and unstable saddle nodes. The phase portraits of the carbon nanotubeactuator exhibit periodic and homoclinic orbits.

Efficacy of High Dose Radiotherapy in Post-operative Treatment of Glioblastoma Multiform - A Single Institution Report

Pashaki, Abdolazim Sedighi,Hamed, Ehsan Akbari,Mohamadian, Kamal,Abassi, Mohammad,Safaei, Afsane Maddah,Torkaman, Tayebe Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.6

Background: Glioblastoma multiform (GBM) is a highly aggressive tumor with median survival of approximately 14 months. Management consists of maximal surgical resection followed by post-operative chemoradiation with concurrent then adjuvant temozolamide. The standard radiotherapy dose is 60Gy in 2-Gy fractions recommended by the radiation therapy oncology group (RTOG). With the vast majority of tumor recurrences occurring within the previous irradiation field and the poor outcome associated with standard therapy, regimens designed to deliver higher radiation doses to improve local control and enhance survival are needed. In this study, we report a single institutional experience in treatment of 68 consecutive patients with GBM, treated with resection, and given post-operative radiotherapy followed by concurrent and/or adjuvant chemotherapy. Results: Of the 80 patients who entered this study, 68 completed the treatment course; 45 (66.2%) males and 23 (33.8%) females with a mean age at diagnosis of $49.0{\pm}12.9$ (21-75) years. At a median follow up of 19 months, 39 (57.3%) patients had evidence of tumor progression and 36 (52.9%) had died. The median over all survival for all patients was 16 months and progression free survival for all patients was 6.02 months. All potential prognostic factors were analyzed to evaluate their effects on overall survival. Age ${\leq}50$ year, concurrent and adjuvant chemotherapy and extent of surgery had significant p values. We found lower progression rate among patients who received higher doses of radiotherapy (>60Gy). Higher radiation doses improved progression free survival (p=0.03). Despite increasing overall survival, this elevation was not significant. Conclusions: This study emphasize that higher radiation doses of (>60Gy) can improve local control and potentially survival, so we strongly advise prospective multi centric studies to evaluate the role of higher doses of radiotherapy on GBM patient outcome.

Nonlocal vibration of carbon/boron-nitride nano-hetero-structure in thermal and magnetic fields by means of nonlinear finite element method

Hamid M. Sedighi,Mohammad Malikan,Ali Valipour,Krzysztof Kamil Zur 한국CDE학회 2020 Journal of computational design and engineering Vol.7 No.5

Hybrid nanotubes composed of carbon and boron-nitride nanotubes have manifested as innovative building blocks to exploit the exceptional features of both structures simultaneously. On the other hand, by mixing with other types of materials, the fabrication of relatively large nanotubes would be feasible in the case of macroscale applications. In the current article, a nonlinear finite element formulation is employed to deal with the nonlocal vibrational behavior of carbon/boron-nitride nano-hetero-tubes in the presence of magneto-thermal environment. Euler–Bernoulli beam model in conjunction with the Eringen’s nonlocal theory of elasticity is adopted to derive the governing equation of motion. In order to conduct a nonlinear frequency analysis, the von-K´arm´an nonlinearity associated with moderate rotations is also considered. It is well known that temperature gradients can significantly change the dynamic behavior of nanotubes. On the other hand, the coefficients of thermal expansions of carbon and boron-nitride nanotubes are quite different that may affect the structural stability of hybrid nanotubes. Hence, to explore the vibration characteristic of such composite structures, the influence of magneto-thermal environment is also taken into account. Finally, the eigenvalue analysis is performed to exhibit the nonlinear mode shapes and natural frequencies of the system due to initial displacement. It is expected that the recognition of dynamic behavior of such hybrid nanotubes may open the doors to the creative design of next-generation nano-devices.