Association of Two Polymorphisms of DNA Polymerase Beta in Exon-9 and Exon-11 with Ovarian Carcinoma in India

Khanra, Kalyani,Panda, Kakali,Bhattacharya, Chandan,Mitra, A.K.,Sarkar, Ranu,Bhattacharyya, Nandan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Background: DNA polymerase beta ($pol{\beta}$) is a key enzyme in the base excision repair pathway. It is 39kDa protein, with two subunits, one large subunit of 31 kDa having catalytic activity between exon V to exon XIV, and an 8 kDa smaller subunit having single strand DNA binding activity. Exons V to VII have double strand DNA binding activity, whereas exons VIII to XI account for the nucleotidyl transferase activity and exons XII to XIV the dNTP selection activity. Aim: To examine the association between $pol{\beta}$ polymorphisms and the risk of ovarian cancer, the present case control study was performed using 152 cancer samples and non-metastatic normal samples from the same patients. In this study, mutational analysis of $pol{\beta}$ genomic DNA was undertaken using primers from exons IX to XIV - the portion having catalytic activity. Results: We detected alteration in DNA polymerase beta by SSCP. Two specific heterozygous point mutations of $pol{\beta}$ were identified in Exon 9:486, A->C (polymorphism 1; 11.18%) and in Exon 11:676, A->C (polymorphism 2; 9.86%). The correlation study involving polymorphism 1 and 4 types of tissue showed a significant correlation between mucinous type with a Pearson correlation value of 4.03 (p=0.04). The association among polymorphism 2 with serous type and stage IV together have shown Pearson ${\chi}^2$ value of 3.28 with likelihood ratio of 4.4 (p=0.07) with OR =2.08 (0.3-14.55). This indicates that there is a tendency of correlation among polymorphism 2, serous type and stage IV, indicating a risk factor for ovarian cancer. Conclusion: Hence, the results indicate that there is a tendency for $pol{\beta}$ polymorphisms being a risk factor for ovarian carcinogenesis in India.

Relationship Between Heat Unit Requirement and Growth and Yield of Mulberry, Morns indica L.

Sarkar A.,Rekha M.,Keshavacharyulu K. Korean Society of Sericultural Science 2005 International Journal of Industrial Entomology Vol.10 No.1

Growth and development of a plant over a growing season is closely related to the daily accumulation of heat. Heat unit measured by accumulated growing degree days (GDD) is becoming increasingly popular to estimate the growth of a plant or even in insect. GDD or heat accumulation per day is measured by calculating average daily temperature and then subtracting the base temperature below which growth does not occur. Heat accumulation per day is added for the desired period and accumulated GDD is determined. The present study was conducted in five seasons in an established garden with K-2, S-36 and V-1 mulberry varieties belonging to Morus indica L. grown under completely irrigated condition at the farm of CSRTI, Mysore during 2001 - 2002. Plants were pruned in each season and the growth of the plant measured by total shoot length and fresh leaf yield was recorded at an interval of 5 days starting from 30 days of pruning (DAP) to 70 days when all the plants were pruned. The accumulated GDD for the corresponding days were recorded and used for analysis. Accumulated growing degree days (GDD) have been found to be perfectly correlated with both growth and yield in all the seasons in all the varieties studied. The high $R^2$ values indicated a strong relationship between the accumulated GDD and, growth and yield of mulberry.

Estimation of Suitable Methodology for Determining Weibull Parameters for the Vortex Shedding Analysis of Synovial Fluid

Singh, Nishant Kumar,Sarkar, A.,Deo, Anandita,Gautam, Kirti,Rai, S.K. The Korean Society of Medical and Biological Engin 2016 의공학회지 Vol.37 No.1

Weibull distribution with two parameters, shape (k) and scale (s) parameters are used to model the fatigue failure analysis due to periodic vortex shedding of the synovial fluid in knee joints. In order to determine the later parameter, a suitable statistical model is required for velocity distribution of synovial fluid flow. Hence, wide applicability of Weibull distribution in life testing and reliability analysis can be applied to describe the probability distribution of synovial fluid flow velocity. In this work, comparisons of three most widely used methods for estimating Weibull parameters are carried out; i.e. the least square estimation method (LSEM), maximum likelihood estimator (MLE) and the method of moment (MOM), to study fatigue failure of bone joint due to periodic vortex shedding of synovial fluid. The performances of these methods are compared through the analysis of computer generated synovial fluidflow velocity distribution in the physiological range. Significant values for the (k) and (s) parameters are obtained by comparing these methods. The criterions such as root mean square error (RMSE), coefficient of determination ($R^2$), maximum error between the cumulative distribution functions (CDFs) or Kolmogorov-Smirnov (K-S) and the chi square tests are used for the comparison of the suitability of these methods. The results show that maximum likelihood method performs well for most of the cases studied and hence recommended.

Isoelectronic Pt(ii)- and Au(iii)-N-heterocyclic carbene complexes: a structural and biological comparison

Rana, Bidyut Kumar,Mishra, Snehasis,Sarkar, Deblina,Mondal, Tapan Kumar,Seth, Saikat K.,Bertolasi, Valerio,Das Saha, Krishna,Bielawski, Christopher W.,Isab, Anvarhusein A.,Dinda, Joydev The Royal Society of Chemistry 2018 NEW JOURNAL OF CHEMISTRY Vol.42 No.13

<P>To elucidate the intrinsic biological activities displayed by platinum <I>versus</I> gold-based organometallic reagents, the isoelectronic and isostructural square planar complexes [Pt(C∩N)(C)Cl][PF6] (2) and [Au(C∩N)(C)Cl][PF6]2 (3), which are supported by a common N-heterocyclic carbene, 1-methyl-2-pyridin-2-yl-2<I>H</I>-imidazo[1,5-<I>a</I>]pyridin-4-ylidene (1), were synthesized. The complexes were characterized using a range of spectroscopic techniques as well as single crystal X-ray diffraction and a series of computational studies were also performed to gain additional insight into their fundamental electronic bonding structures. The cytotoxicities of the complexes were tested <I>in vitro</I> against non-small lung carcinoma (A549), colorectal carcinoma (HCT-116) and human hepatocellular carcinoma (HepG2) cell lines. The measured IC50 values showed that the Au(iii)-NHC complex is more potent than its Pt analogue.</P>

Electro-Optical Characterization of nanoGUMBOS

A. Sarkar,K. Kanakamedala,M. D. Rajathadripura,N. N. Jagadish,P. K. S. Magut,S. de Rooy,S. Das,B. El-Zahab,I. M. Warner,T. Daniels-Race 대한금속·재료학회 2014 ELECTRONIC MATERIALS LETTERS Vol.10 No.4

Molecular electronics, where nanoscale organic species are utilized as active electronic components, offers a promising approach towards ultimate miniaturization and integration of hybrid electronic materials (HEMs) with traditional silicon based complementary metal oxide semiconductors (CMOS) technology. Toward this end, fundamental research studies to understand the electronic and optical properties of these molecules are of paramount importance. In this work, conductive probe atomic force microscopy (CP-AFM) and Raman spectroscopy have been performed on ionic liquid based unique organic nanoparticles derived from a Group of Uniform Materials Based on Organic Salts (GUMBOS). Aptly named as nanoGUMBOS, the material investigated in this report is Rhodamine6G tetraphenylborate ([R6G][TPB]) as has been synthesized by a room temperature facile metathesis reaction between Rhodamine 6G chloride (R6GCl) and sodium tetraphenylborate (NaTPB) followed by an ultrasonication- assisted, additive-free, re-precipitation reaction. To the best of our knowledge, the results reported herein are first-time evidence of electrical performance exhibited by [R6G][TPB] nanoGUMBOS. In conjunction with the supportive results of Raman spectra, the current-voltage (I-V) characteristics obtained are indicative of the potential incorporation of this unique compound in hybrid electronics with respect to potential applications in optoelectronics and chemical sensing.

Gravimetrics of Pupal Weight Loss in the Domestic Silkworm, Bombyx mori Linn. (Lepidoptera : Bombycidae)

Kumar, Vineet,Kariappa, B.K.,Chaturvedi, H.K.,Sarkar, A.,Datta, R.K. Korean Society of Sericultural Science 2000 International Journal of Industrial Entomology Vol.1 No.1

A comprehensive study on daily pupal weight loss due to histolysis and histgenesis in Bombyx mori Linn., has been undertaken. The percentag of pupal weight loss in the male pupa is higher than the female, conforming that the female pupa require more energy in the form of less pupal weight loss for ovipositional activities. The regression equation clearly shows higher percentage loss of daily pupal weight in male than female with respect to age and, this is also evident from the slope of the regression line. Moreover, analysis of the coefficient of correlation shows that the weight loss of pupa is directly correlated with the age rather than climatic parameters under which the pupa dwells.

Research Articles : Heterobeltiotic Genetic Interaction between Congenic and Syngenic Breeds of Silkworm, Bombyx mori L.

( A. K. Verma ),( G. K. Chattopadhyay ),( M. Sengupta ),( S. K. Das ),( A. Sarkar ) 한국잠사학회 2005 International Journal of Industrial Entomology Vol.11 No.2

Variability in Specific Leaf Weight in Mulberry Germplasm and Its Inheritance Pattern

Sarkar, A.,Mogili, T.,Chaturvedi, H.K. Korean Society of Sericultural Science 2003 International Journal of Industrial Entomology Vol.7 No.1

Specific leaf weight (SLW), defined as the mass of tissue per unit leaf area has been found to be an important physiological parameter as it indicates the relative thickness of leaves. Greater SLW provides more photosynthetic potential per unit area of leaf and hence it is frequently been considered as correlated with photosynthesis in several plant species. Collections of 165 mulberry (Morus sp.) germplasm accessions, both Indian and exotic in origin were evaluated for their variability with respect to SLW. The mean specific leaf weight ranged from 35.3 to $72.3 g/m^{-2}$. The distribution of SLW was found to be normal. High heritability (97.08%) and a small difference between genotypic and phenotypic variance demonstrates the genetic control over SLW. Significant heterotic effect with respect to SLW was observed in crosses when parents with high and low SLW were chosen.

A Novel Approach for Accurate Assessment of Design Wind Speed for Variable Wind Climate

G. K. Gugliani,A. Sarkar,S. Bhadani,S. Mandal 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.2

For a widely varied wind climate in India, a single type of Extreme Value Distribution (EVD) is not suitable to fit wind speed data for all geographical locations and climatic conditions. This may lead to the inappropriate estimation of the design wind speed (Vd). In this study, a new approach, combining the merits of both the block maxima and the peaks over threshold approaches, has been used to determine the Vd. The hourly mean wind speed data of three stations, viz., Trivandrum, Bombay and Calcutta have been classified into block of months of the years 1973−2005, 1969−2007, and 1969−1994 respectively. The extreme peaks over the appropriate threshold value have been fitted into the generalized EVD (type-I, II and II). The results indicate that Fréchet distribution is the most suitable distribution for all three stations. March has the highest Vd for Trivandrum, whereas, for both Bombay and Calcutta it is September. Bombay shows highest overestimation of the Vd as given in Indian code of standard, whereas, for Trivandrum, and Calcutta these deviations have been found to be comparatively less. The five plotting position formulae have also been compared based on two goodness of fit tests, viz., R2, and RMSE.

Heterobeltiotic Genetic Interaction between Congenic and Syngenic Breeds of Silkworm, Bombyx mori L.

Verma A. K.,Chattopadhyay G. K.,Sengupta M.,Das S. K.,Sarkar A. K. Korean Society of Sericultural Science 2005 International Journal of Industrial Entomology Vol.11 No.2

To determine the level of heterosis, higher cocoon shell weight multivoltine congenic lines (Con. L) and bivoltine syngenic lines (Syn. L) of silkworm were used for crosses. First filial generations $(F_1s)$ expressed heterobeltiotic genetic interaction at significant magnitude (p < 0.01) for single cocoon shell weight (SCSW). The other linked characters viz., single cocoon weight (SCW) and yield by weight per 10, 000 larvae were also significantly higher (p < 0.01) than the better parental lines. All the hybrids showed significant improvement for these aforesaid characters over standard heterosis (Standard check). The reeling parameters viz., filament length, raw silk, neatness, cohesionstrokes etc, also showed improvement among the hybrids than check in congenial environment. Overall results suggested that the cross between congenic and syngenic lines provide better heterosis with good quality silk than conventional hybrids and may be used for commercial exploitation.