The Effects of Skin Care of Extracts from Different Parts of Camellia sinensis

Sang Hwi Im(임상휘),Ju Sung Kim(김주성) 한국약용작물학회 2022 한국약용작물학회 학술대회논문집 Vol.2022 No.1

차나무 부위별 추출물의 이화학적 특성

임상휘(Sang Hwi Im),김주성(Ju Sung Kim) 한국약용작물학회 2022 한국약용작물학회지 Vol.30 No.3

Background: There are various physiologically active substances in Camellia sinensis leaves, and much research has been conducted on it, however, the properties of other parts such as branch, flower, seed, and root are still unknown. Therefore, in this study, we checked the physicochemical properties of each part of C. sinensis. Methods and Results: In this study, we prepared a 70% ethyl alcohol extract of each part of C. sinensis and determined their yield, total polyphenol and total flavonoid contents, antioxidant activity, components [using high performance liquid chromatography (HPLC)], and α-glucosidase inhibitory activity. The yield was highest in the flower extract, and the total polyphenol content and antioxidant activity tended to be high in the order of leaf, root, branch, flower, and seed. Analysis of the catechin compounds using HPLC, confirmed that epigallocatechin (EGC) and epigallocatechin gallate (EGCG) were mostly present in the leaf, but epicatechin (EC), gallocatechin gallate (GCG), and gallocatechin (GC) were present in varying concentrations in each part. The inhibitory activity of α-glucosidase was higher than that of acarbose, a hypoglycemic agent, in all parts except the seed, which showed no activity. Furthermore, significantly higher α-glucosidase inhibitory activity was confirmed in the root. Conclusions: Apart from the leaves, which have been widely used and studied in the industry, the C. sinensis root and brances showed high activity. In particular, the α-glucosidase inhibitory activity was very high, confirming the possibility of using the roots and brances discarded by the tea leaf industry as an antidiabetic material.

아로니아 첨가 비율에 따른 프리저브 잼의 품질 특성 및 항산화 활성

임상휘(Sang Hwi Im),김경철(Kyeoung Cheol Kim),김주성(Ju-Sung Kim) 한국식품과학회 2022 한국식품과학회지 Vol.54 No.2

본 연구는 생리활성물질이 풍부한 아로니아를 활용하여 가공식품 개발에 필요한 기초 연구 데이터를 제공하기 위해 아로니아와 설탕의 배합비율별 프리저브 잼을 제조한 뒤 그 품질 특성과 항산화 활성을 조사하였다. 배합비율에 따라 아로니아의 함량이 낮아질수록 당도는 높아졌으며 수분함량은 낮아지는 경향을 나타내었다. 강도와 최대응력은 아로니아 함량이 줄어들고 설탕의 함량이 증가할수록 높은 값을 나타내었다. 잼의 비율별 항산화 활성은 아로니아 함량 70%에서 DPPH radical 저해율이 가장 높았다. 총 페놀 함량, TEAC, FRAP, ORAC의 값은 모두 아로니아 함량에 따라 비례적으로 증가하는 경향을 나타내었다. 관능평가에서 단맛과 식감이 모두 강하다고 평가된 아로니아 함량 30%에서는 오히려 종합적 선호도에서 가장 낮게 나타났으며 아로니아 함량 40%에서 가장 높은 선호도를 나타내었다. 본 연구에서는 아로니아와 설탕 배합비율에 따른 품질 특성과 항산화 연구를 진행하였으며 국산 아로니아를 활용한 제품의 개발에 기초 자료로서 활용될 것으로 기대된다. This study investigated the quality characteristics and antioxidant activities of preserved jams prepared by mixing various ratios of aronia and sugar. To analyze the quality characteristics, the total sugar, pH, moisture, maximum stress, hardness, total phenolics, 1,1-diphenyl-2-picrylhydrazyl (DPPH), trolox-equivalent antioxidant capacity (TEAC), ferric reducing antioxidant power (FRAP), oxygen radical absorbance capacity (ORAC), and sensory characteristics of the preserved jams were measured. Lower aronia contents resulted in higher total sugar content, hardness, and strength, as well as lower moisture, total phenolic content, and DPPH, TEAC, FRAP, and ORAC values. In terms of the sensory characteristics, an aronia content of 30%, which was determined to have strong sweetness and texture, resulted in the lowest overall acceptability. In contrast, the highest preference was shown to an aronia content of 40%. In this study, quality characteristics and antioxidant activity experiments were conducted based on various ratios of aronia and sugar. The results are expected to be used as preliminary data for developing products that use domestic aronia.

녹나무 잎의 반응표면분석법을 이용한 환류냉각 추출조건 최적화 및 생리활성

임상휘 ( Sang Hwi Im ),김경철 ( Kyeoung Cheol Kim ),김지호 ( Ji Ho Kim ),박미현 ( Mi Hyeon Park ),김주성 ( Ju-sung Kim ) 조선대학교 공학기술연구원 2021 공학기술논문지 Vol.14 No.3

Experiments were conducted to increase the efficiency of extracting bioactive compounds from Cinnamomum camphora leaves which are industrially used in various fields. Response surface methodology (RSM) maximized the total polyphenol content in extracting C. camphora leaves. The experimental design was conducted for three factors: extraction temperature, ethanol concentration, and solid-liquid ratio. The optimum conditions for the total polyphenol content of C. camphora leave extract were found to be an extraction temperature of 80℃, an ethanol concentration of 55%, and a solid ratio of 100; the predicted value was confirmed to be 57.67 ± 0.66 mg GAE/g. As a result of the experiment, the total polyphenol content of the optimized extract of C. camphora leaves was 57.29 ± 0.41 mg GAE/g, and the total flavonoid content was 10.57 ± 0.20 mg QE/g. The antioxidant activity was also measured at 25.98 ± 0.67 μg/mL for DPPH RC50, higher than the control group BHT (122.69 ± 10.55 μg/mL). TEAC was 239.39 ± 11.28 μM TE/g, and FRAP was 2,049.62 ± 32.45 mM FE/g. Moreover, the inhibitory effect of tyrosinase and Staphylococcus epidermidis was confirmed. The results of this study suggest the optimal conditions for maximum extraction of total polyphenol content from C. camphora leaves, and it is expected to be essential data for industrial use through the physiological activity of optimized extracts.

차나무 부위별 추출물의 미백, 주름 개선과 피부보호 효과

임상휘(Sang Hwi Im),김주성(Ju Sung Kim) 한국약용작물학회 2022 한국약용작물학회지 Vol.30 No.5

Background: Camellia sinensis is globally cultivated, principally for the leaves which are used as a green tea. Green tea made from C. sinensis leaves contains various physiologically active ingredients and is used for health and beauty purposes; however, most parts of the plant are discarded. In this experiment the physiological activity of each part of the C. sinensis was analyzed to confirm its potential for use as a cosmetic raw material. Methods and Results: Leaves, flowers, roots, branches, and seeds were extracted with 70% ethyl alcohol. Experiments were performed to measure, tyrosinase inhibitory activity, elastase inhibitory activity, antibacterial activity, and cell viability upon ultraviolet B (UVB)-induced cell damage. Tyrosinase inhibitory activity was high in leaves, flowers, and roots, and elastase inhibitory activity showed high inhibition rates in roots, leaves, and branches. Antibacterial activity against Propionibacterium acnes was highest in leaves and roots, followed by branches and flowers, with no antibacterial activity found in seeds. In terms of the protective effect from UVB irradiation induced damage, the experimental group treated with the seed extract in HaCaT cells showed a high survival rate. In Detroit cells, an increased survival rate was observed in most of the experimental groups treated with the extracts, particularly those treated with the seed and flower extracts. Conclusions: Experiment results suggest various parts of the C. sinensis, could be used as raw materials for cosmetics.

침엽수 잎과 구과 추출물의 생리활성

강소담,임상휘,김주성,Sodam Kang,Sang Hwi Im,Ju-Sung Kim 한국식물생명공학회 2023 JOURNAL OF PLANT BIOTECHNOLOGY Vol.50 No.1

침엽수는 국내에 널리 분포되어 있고 산업계에서 다양한 방법으로 이용하고 있다. 하지만 대부분의 연구는 한정적으로 진행되었으며 침엽수 간의 비교 분석에 대한 연구는 상대적으로 소홀히 다루어져 왔다. 이에 본 연구에서는 국내에 분포하는 침엽수 4종을 선정한 후 생리활성을 측정하여 침엽수 간의 효능을 비교하였다. 실험에 앞서 소나무, 편백나무, 삼나무, 구상나무의 잎과 구과를 채취하였고 70% 메탄올로 추출하였다. 실험은 총 페놀 및 플라보노이드 함량, 항산화 활성(DPPH, TEAC, FRAP), α-glucosidase, elastase, tyrosinase 효소 저해 활성을 진행하였다. 총 페놀 및 플라보노이드 함량, 항산화 활성에서는 편백나무와 삼나무에서 높게 측정되었다. α-Glucosidase 저해활성에서는 편백나무 잎의 활성이 가장 높아 항당뇨 제품으로의 활용성이 높은 것으로 확인되었다. Elastase 저해활성에서는 소나무 잎과 구과, 구상나무구과에서 높게 나타나 주름개선 화장품으로의 이용 가능성을 확인하였고, 미백실험인 tyrosinase 저해활성에서는 소나무 잎에서 활성이 가장 높게 측정되었다. 우리나라는 침엽수를 가로수와 방풍림 조성 등의 목적으로 대량 식재하고 있고, 매년 가지치기 작업을 해주기 때문에 원료 수급에 있어서 경쟁력이 높은 편이다. 그렇기 때문에 본 실험 결과를 기반으로 헬스케어 제품, 당뇨 제품, 주름 및 미백 화장품 등으로 개발하기에 적합할 것으로 판단된다. Although softwoods are widely distributed in Korea and used in various industries, studies are limited and comparative research on softwoods has been overlooked. Therefore, in this study, the physiological activity of four species of softwoods found in Korea was quantified. Needles and cones of Pinus densiflora, Chamaecyparis obtusa, Cryptomeria japonica, and Abies koreana were collected, and material was extracted using 70% methanol. We quantified the following physiological traits: total phenol and flavonoid content, antioxidant activity (DPPH, TEAC, FRAP), and inhibitory activity of the enzymes α-glucosidase, elastase, and tyrosinase. Total phenol and flavonoid contents and antioxidant activities were high in Chamaecyparis obtusa and Cryptomeria japonica, and α-glucosidase inhibition activity was highest in the leaves of Chamaecyparis obtusa. Elastase inhibitory activity was high in the leaves and cones of Pinus densiflora and the cones of Abies koreana, and tyrosinase inhibitory activity was highest in the leaves of Pinus densiflora. In Korea, softwoods are planted extensively on roadsides and as windbreaks, and they are highly competitive in supplying raw materials since they are pruned every year. Based on the results of this study, softwoods are considered suitable for use in healthcare products, diabetes products, wrinkles, and whitening cosmetics.

침엽수 잎과 구과 추출물의 생리활성

강소담(Sodam Kang),임상휘(Sang Hwi Im),김주성(Ju-Sung Kim) (사)한국식물생명공학회 2023 JOURNAL OF PLANT BIOTECHNOLOGY Vol.50 No.4

Polyphenol Content of Cinnamomum camphora according to Extraction Conditions

Mi Hyeon Park(박미현),Ji Ho Kim(김지호),Sang Hwi Im(임상휘),Kyeong Cheol Kim(김경철),Ju Sung Kim(김주성) 한국약용작물학회 2020 한국약용작물학회 학술대회논문집 Vol.2020 No.1

녹나무 잎 추출 조건에 따른 폴리페놀 함량과 항산화활성

김지호, 임상휘, 박미현, 김경철, 김주성 충북대학교 농업과학기술연구소 2021 農業科學硏究 Vol.37 No.1

본문참고

에탄올 농도별 황금 추출물의 총 페놀, 총 폴라보노이드 및 황산화 활성

황현우, 임상휘, 김주성 충북대학교 농업과학기술연구소 2023 農業科學硏究 Vol.39 No.1

본문참고