Phytochemical Screening and Biological Studies of Boerhavia Diffusa Linn

Prakriti Gautam, Sandesh Panthi, Prashubha Bhandari, Jihoon Shin, Jin Cheol Yoo 조선대학교 기초과학연구원 2016 조선자연과학논문집 Vol.9 No.1

Hexane, ethyl acetate and methanol extracts of whole plant of Boerhavia diffusa were screened for phytochemical and biological activities. Qualitative phytochemical screening via colorimetric method and the quantitative estimation of phenolic and flavonoid content were performed. Antioxidant assay using DPPH scavenging method was studied. Antimicrobial screening of plant extracts was done by cup diffusion technique. Cytotoxic activity of B. diffusa was studied by brine shrimp bioassay and anthelminthic activity was evaluated in vitro in Pheretima posthuma. This study revealed B. diffusa as a source of various phyto-constituents such as alkaloids, glycosides, saponins, tannins, carbohydrates, cardiac glycosides, flavonoids and terpenoids. Quantitative estimation of total phenol was found to be maximum in BEE i.e. 29.73±0.88, BME 19.8±2.02 and in BHE 9.15±0.304 mgGAE/g. Similarly, the total flavonoid content was found to be 17.44±0.75 in BEE, 14.43±0.23 in BHE and 3.678 mg QE/g in BME. Ethyl acetate extract showed its antibacterial activity against all tested pathogens except Escherichia coli whereas Staphylococcus aureus and Salmonella Typhi were resistant to methanol and hexane extract. The zone of inhibition (ZOI) of ethyl acetate extract against S. Typhi and B. cereus was found to be 18 mm and 14 mm respectively. The MIC value of BEE in S. Typhi was 3.125 μg/ml and in B. cereus was 12.5 μg/ml. The preliminary screening of anticancer property of B. diffusa i.e. BSLT in methanol was found to be 165.19 μg/ml. B. diffusa was also found to contain anthelmintic property. The study helped in further exploration of medicinal properties of B. diffusa by phytochemical screening and biological activities paving the path for study and investigation in this plant.

Anatomical distributional defects in mutant genes associated with dominant intermediate Charcot-Marie-Tooth disease type C in an adenovirus-mediated mouse model

Lee, SeoJin,Panthi, Sandesh,Jo, Hyun Woo,Cho, Jaeyoung,Kim, Min-Sik,Jeong, Na Young,Song, In Ok,Jung, Junyang,Huh, Youngbuhm Medknow PublicationsMedia Pvt Ltd 2017 Neural regeneration research Vol.12 No.3

<P>Dominant intermediate Charcot-Marie-Tooth disease type C (DI-CMTC) is a dominantly inherited neuropathy that has been classified primarily based on motor conduction velocity tests but is now known to involve axonal and demyelination features. DI-CMTC is linked to tyrosyl-tRNA synthetase (YARS)-associated neuropathies, which are caused by E196K and G41R missense mutations and a single <I>de novo</I> deletion (153-156delVKQV). It is well-established that these YARS mutations induce neuronal dysfunction, morphological symptoms involving axonal degeneration, and impaired motor performance. The present study is the first to describe a novel mouse model of YARS-mutation-induced neuropathy involving a neuron-specific promoter with a deleted mitochondrial targeting sequence that inhibits the expression of YARS protein in the mitochondria. An adenovirus vector system and <I>in vivo</I> techniques were utilized to express YARS fusion proteins with a Flag-tag in the spinal cord, peripheral axons, and dorsal root ganglia. Following transfection of YARS-expressing viruses, the distributions of wild-type (WT) YARS and E196K mutant proteins were compared in all expressed regions; G41R was not expressed. The proportion of Flag/green fluorescent protein (GFP) double-positive signaling in the E196K mutant-type mice did not significantly differ from that of WT mice in dorsal root ganglion neurons. All adenovirus genes, and even the empty vector without the YARS gene, exhibited GFP-positive signaling in the ventral horn of the spinal cord because GFP in an adenovirus vector is driven by a cytomegalovirus promoter. The present study demonstrated that anatomical differences in tissue can lead to dissimilar expressions of YARS genes. Thus, use of this novel animal model will provide data regarding distributional defects between mutant and WT genes in neurons, the DI-CMTC phenotype, and potential treatment approaches for this disease.</P>

Phytochemical Screening and Biological Studies of Boerhavia Diffusa Linn

Gautam, Prakriti,Panthi, Sandesh,Bhandari, Prashubha,Shin, Jihoon,Yoo, Jin Cheol The Basic Science Institute Chosun University 2016 조선자연과학논문집 Vol.9 No.1

Hexane, ethyl acetate and methanol extracts of whole plant of Boerhavia diffusa were screened for phytochemical and biological activities. Qualitative phytochemical screening via colorimetric method and the quantitative estimation of phenolic and flavonoid content were performed. Antioxidant assay using DPPH scavenging method was studied. Antimicrobial screening of plant extracts was done by cup diffusion technique. Cytotoxic activity of B. diffusa was studied by brine shrimp bioassay and anthelminthic activity was evaluated in vitro in Pheretima posthuma. This study revealed B. diffusa as a source of various phyto-constituents such as alkaloids, glycosides, saponins, tannins, carbohydrates, cardiac glycosides, flavonoids and terpenoids. Quantitative estimation of total phenol was found to be maximum in BEE i.e. $29.73{\pm}0.88$, BME $19.8{\pm}2.02$ and in BHE $9.15{\pm}0.304mgGAE/g$. Similarly, the total flavonoid content was found to be $17.44{\pm}0.75$ in BEE, $14.43{\pm}0.23$ in BHE and 3.678 mg QE/g in BME. Ethyl acetate extract showed its antibacterial activity against all tested pathogens except Escherichia coli whereas Staphylococcus aureus and Salmonella Typhi were resistant to methanol and hexane extract. The zone of inhibition (ZOI) of ethyl acetate extract against S. Typhi and B. cereus was found to be 18 mm and 14 mm respectively. The MIC value of BEE in S. Typhi was $3.125{\mu}g/ml$ and in B. cereus was $12.5{\mu}g/ml$. The preliminary screening of anticancer property of B. diffusa i.e. BSLT in methanol was found to be $165.19{\mu}g/ml$. B. diffusa was also found to contain anthelmintic property. The study helped in further exploration of medicinal properties of B. diffusa by phytochemical screening and biological activities paving the path for study and investigation in this plant.

Phytochemical Screening and Biological Studies of Boerhavia Diffusa Linn

Prakriti Gautam,Sandesh Panthi,Prashubha Bhandari,Jihoon Shin,Jin Cheol Yoo 조선대학교 기초과학연구원 2016 조선자연과학논문집 Vol.9 No.1

Purification and characterization of chitinase showing antifungal and biodegradation properties obtained from Streptomyces anulatus CS242

Poonam Mander,조승식,최윤희,Sandesh Panthi,최윤석,김환묵,유진철 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.7

In an effort to identify a microbial enzyme thatcan be useful as a fungicide and biodegradation agent ofchitinous wastes, a chitinase (Chi242) was purified fromthe culture supernatant of Streptomyces anulatus CS242utilizing powder of shrimp shell wastes as a sole carbonsource. It was purified employing ammonium sulfate precipitationand gel permeation chromatography techniques. The molecular weight of the purified chitinase was*38 kDa by SDS-PAGE. The N-terminal amino acidsequence (A-P-G-A-P-G-T-G-A-L) showed close similarityto those of other Streptomyes chitinases. The purifiedenzyme displayed optimal activity at pH 6.0 and 50 Crespectively. It showed substantial thermal stability for 2 hat 30–60 C, and exhibited broad pH stability in the range5.0–13.0 for 48 h at 4 C. Scanning electron microscopyconfirmed the ability of this enzyme to adsorb onto solidshrimp bio-waste and to degrade chitin microfibers. Chi242could proficiently convert colloidal chitin to N-acetyl glucosamine(GlcNAc) and N-acetyl chitobiose (GlcNAc)2signifying that this enzyme is suitable for bioconversion ofchitin waste. In addition, it exerted an effective antifungalactivity towards fungal pathogen signifying its role as abiocontrol agent. Thus, a single microbial cell of Streptomycesanulatus CS242 justified its dual role.