Genetic Variability of mtDNA Sequences in Chinese Native Chicken Breeds

Liu, Z.G.,Lei, C.Z.,Luo, J.,Ding, C.,Chen, G.H.,Chang, H.,Wang, K.H.,Liu, X.X.,Zhang, X.Y.,Xiao, X.J.,Wu, S.L. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.7

The variability of mtDNA hypervariable segment I (HVS I) sequences was investigated in a total of 48 birds belonging to 12 Chinese native chicken breeds. Sixteen haplotypes were identified from 35 polymorphic nucleotide sites which accounted for 6.4% of a sequenced 544 bp fragment. Diversity analysis of the haplotypes showed that Tibetan, Langshan and Henan cockfight chicken had only one haplotype, while ancient haplotypes existed in Taihe silky and Chahua chicken. Phylogenetic analysis of the haplotypes suggested that Chinese native chicken breeds shared 5 maternal lineages and some breeds would share the same maternal lineage, regardless of their external features and ecological types. Both divergent and phylogenetic analysis of the haplotypes indicated the close genetic relationships between the Chinese native chicken breeds and G. g. gallus and G. g. spadiceus from different areas, which implied that G. g. gallus and G. g. spadiceus were the original ancestors of the Chinese native chicken breeds.

Evaluation of Sulfur and Multi-Walled Carbon Nanotube Composite Synthesized by Dissolution and Precipitation for Li/S Batteries

Park, J. S.,Kim, D. J.,Park, J. W.,Ryu, H. S.,Kim, K. W.,Wang, G. X.,Ahn, H. J. American Scientific Publishers 2012 Journal of Nanoscience and Nanotechnology Vol.12 No.7

<P>An elemental sulfur and multi-walled carbon nanotube (S-MWNT) composite was synthesized by dissolving sulfur in ammonium sulfides and then precipitating on MWNT. Morphology observation by scanning electron microscopy (SEM) confirmed that S-MWNT product was successfully prepared by incorporating sulfur into MWNT network. Without additional conducting material, the S-MWNT composite cathodes were prepared for electrochemical tests. The properties measured in discharge-charge cycling test showed that the composite had the initial discharge capacity of 1024 mAh g(-1), which is about 61% sulfur utilization. However, in the subsequent cycling, the capacities faded. To determine the reason of rapid capacity drop, S-MWNT composite cathodes were compared in the cycling tests with varying three kinds of electrolytes and the cathode was subjected to physical force by rolling. The changes in the cycle performances proved that the deterioration of S-MWNT composite cathodes was not related to the electrolytes but to physical bonding that may not maintain the conducting path between sulfur and MWNT.</P>

Proteomic Analysis Reveals PGAM1 Altering cis-9, trans-11 Conjugated Linoleic Acid Synthesis in Bovine Mammary Gland.

Wang, T,Lee, S B,Hwang, J H,Lim, J N,Jung, U S,Kim, M J,Kang, H S,Choi, S H,Lee, J S,Roh, S G,Lee, H G American Oil Chemists' Society 2015 Lipids Vol.50 No.5

<P>cis-9, trans-11 Conjugated linoleic acid (CLA) is one of the most extensively studied CLA isomers due to its multiple isomer-specific effects. However, the molecular mechanisms of cis-9,trans-11 CLA synthesis in ruminant mammary gland are still not clearly understood. This process may be mediated, to a certain extent, by trans-11 C18:1 regulated by stearoyl-CoA desaturase-1 (SCD1) and/or its syntrophic proteins. This study aimed to investigate the effects of TVA on SCD1-mediated cis-9,trans-11 CLA synthesis in MAC-T cells and its potential molecular mechanism. Results showed that trans-11 C18:1 was continually taken up and converted into cis-9,trans-11 CLA in MAC-T cells during the 4-h incubation of 50?μM trans-11 C18:1. SCD1 protein expression increased more than twofold at 2?h (P?<?0.01) and 2.5?h (P?<?0.05) before decreasing to less than half of the normal level at 4?h (P?<?0.05). One up-regulated (RAS guanyl releasing protein 4 isoform 1 [RASGRP4]) and six down-regulated proteins (glucosamine-6-phosphate deaminase 1 [GNPDA1], triosephosphate isomerase [TPI1], phosphoglycerate mutase 1 [PGAM1], heat shock protein beta-1 [HSPB1], annexin A3 [ANXA3], thiopurine S-methyltransferase [TPMT]) were found in MAC-T cells treated with trans-11 C18:1. Of these seven identified proteins, the presence of GNPDA1 and PGAM1 was verified in several models. More trans-11 C18:1 was taken up after PGAM1 knockdown by small interfering RNA (siRNA). In conclusion, our data suggested that PGAM1 may have a negative relationship with SCD1 and seemed to be involved in cis-9, trans-11 CLA synthesis by facilitating the absorption of trans-11 C18:1 in the bovine mammary gland.</P>

工業高等學校 電子科 敎育方向의 設定

洪鳳植,王鎭錫,吳承燁,金太均,林在鳳,朴東徹,沈殷燮 충남대학교 공업교육연구소 1979 論文集 Vol.2 No.2

In the last ten years there has been a dramatic change in the electrical industry regarding its technology and capability. VLSI circuits technologies have brought about the develoment of computers, digital communications, digital control systems, and others. The microprocessor now is appearing in the simplest appliances as well as in complex systems, permeating all of engineering. This paper is presented as a proposal for a more improved education courses for technical high school students preparing to enter this dynamic electronic industry. The current curricula, and their syllabuses, of the electronic deparments of typical technical high schools were analyzed, and their teachers and instructors, as well as technicians in electronic companies, were consulted about the education courses. The results of this study are follow: 1. The fields of digital circuits and systems using IC and LSI should be emphasized in the current curricula and the subject of microprocessor applications should be added. 2. The current ratio of the theoretical; subject hours vs laboratory /shop practice hours should be rearranged at about same ratio through effective laboratory systems. 3. A retraining course for experimental instructors and supervisors should be provided, similar to that provided for teachers. 4. Technical high school education should avoid the erroneous philosophy of training the student only sufficiently to pass the qualification examination.

MINERAL NUTRITION OF GRAZING SHEEP IN NORTHERN CHINA I. MACRO-MINERALS IN PASTURE, FEED SUPPLEMENTS AND SHEEP

Masters, D.G.,Purser, D.B.,Yu, S.X.,Wang, Z.S.,Yang, R.Z.,Liu, N.,Lu, D.X.,Wu, L.H.,Ren, J.K.,Li, G.H. Asian Australasian Association of Animal Productio 1993 Animal Bioscience Vol.6 No.1

This study determined the macro-mineral levels in plants and sheep, at different times during the year, at three farms in northern China. Samples of plants, animal tissues and faeces were collected at 5 to 8 times during the year from each site. They were analysed for calcium, sodium, phosphorus, magnesium and potassium. Sodium concentrations in plants were below those recommended for optimum animal production at all sites for all or part of the year (0.01-1.66 g/kg DM). Low concentrations of sodium in faeces were measured and signs of sodium deficiency (soil ingestion) were observed on one farm. There were seasonal trends in other mineral levels in plants and animals. Plants were lowest in potassium (2.3-13.4 g/kg DM), magnesium (1.28-4.82 g/kg DM) and phosphorus (0.24-1.62 g/kg DM) in winter and spring. However, high levels of these elements were supplied in the feed supplements used at this time of the year. During the periods of rapid pasture growth, in summer and autumn, supplements of feed and salt are often not provide even though pasture concentrations of phosphorus and sodium are low. It may be at these times that sheep will be most susceptible to deficiencies of these elements.

Rapid and segmental specific dysregulation of AQP2, S256-pAQP2 and renal sodium transporters in rats with LPS-induced endotoxaemia

Olesen, E. T. B.,de Seigneux, S.,Wang, G.,Lutken, S. C.,Frokiaer, J.,Kwon, T.-H.,Nielsen, S. Oxford University Press 2009 Nephrology, dialysis, transplantation Vol.24 No.8

<P>BACKGROUND: Acute renal failure (ARF) is a frequent complication of sepsis. Characteristics of ARF in sepsis are impaired urinary concentration, increased natriuresis and decreased glomerular filtration rate (GFR), in which inducible nitric oxide synthase (iNOS) has been revealed to play a role. Aims. We aimed to investigate renal water and sodium excretion and in parallel the segmental regulation of renal AQP2 and major sodium transporters in rats with acute LPS-induced endotoxaemia. Next, we aimed to examine the changes of iNOS expression and activated macrophage infiltration in the kidney and the effects of iNOS inhibition on AQP2 and NKCC2 expression in LPS rats. METHODS: Rats were treated with LPS (i.p.) or with LPS + iNOS inhibitor L-NIL, and 6 h later kidneys were subjected to semiquantitative immunoblotting and immunohistochemistry. RESULTS: Polyuria and increased natriuresis were seen 6 h after LPS injection alongside downregulation of both AQP2 and S256-phosphorylated AQP2 in CTX/OSOM and ISOM but not in inner medulla (IM). Thick ascending limb sodium transporters NHE3 and NKCC2 were downregulated in ISOM and NaPi2 was decreased in CTX/OSOM, whereas NCC and ENaC were not consistently downregulated. Immunolabelling intensity of iNOS was increased in vascular structures and transitional epithelium, and an infiltration of activated macrophages was seen in CTX and ISOM. L-NIL co-treatment prevented the downregulation of NKCC2 but not AQP2 in LPS rats. CONCLUSIONS: Early downregulation of AQP2 and sodium transporters takes place segmentally in the kidney after LPS administration. In addition, an infiltration of activated macrophages and increased iNOS expression may play a role in the urinary concentrating defect in acute LPS-induced entotoxaemia.</P>

커플링이 고려된 KSTAR ICRF 안테나의 8포트 전송선 회로 모델링 및 측정 결과 비교

김선호,왕선정,황철규,곽종구,Kim, S.H.,Wang, S.J.,Hwang, C.K.,Kwak, J.G. 한국진공학회 2010 Applied Science and Convergence Technology Vol.19 No.1

KSTAR ICRF 시스템에서 안테나 전류띠 간 커플링에 의한 전류띠의 전압, 전류 분포 변화와 전류띠 간 전력 전달에 의한 공명현상 그리고 전송선상의 이상 전압 분포 등을 예측하거나 분석하는 것은 그것의 안정적이고 신뢰성 있는 운전을 위해 매우 중요하다. 본 연구에서는 이러한 전류띠 간 커플링에 의한 현상들을 이해하기 위해 ICRF 안테나에서 측정한 S-parameter를 커플링이 고려된 8포트 전송선회로 모델에 적용하여 전류띠의 전송선 회로 모델을 완성하였다. 완성된 전송선 회로모델의 자체유도계수, 상호유도계수, 전기용량성 등은 전류띠의 유한한 길이로 인하여 2D 모델의 값보다 작은 것으로 나타났다. 커플링이 고려된 전류띠의 전송선 회로모델은 공명루프와 결합되어 있는 KSTAR ICRF 시스템의 운전에 활용될 것이다. It is very important to predict and analyze the change of voltage and current distribution of current strap, abnormal voltage distribution of transmission line and resonance phenomenon by coupling between current straps for more stable operation of ICRF system. In this study, to understand those phenomena by coupling, 8-port coupled transmission line model is completed by appling S-parameter measured in the prototype KSTAR ICRF antenna to the model. The determined self-inductance, mutual-inductance and capacitance of antenna straps are shown to be lower than that calculated from 2D approximate model due to finite length of strap. The coupled transmission line model of current strap will be utilized to the operation of ICRF system of KSTAR in the future.

Disruption of a regulatory loop between DUSP1 and p53 contributes to hepatocellular carcinoma development and progression

Hao, P.P.,Li, H.,Lee, M.J.,Wang, Y.P.,Kim, J.H.,Yu, G.R.,Lee, S.Y.,Leem, S.H.,Jang, K.Y.,Kim, D.G. Elsevier Science Publishers 2015 Journal of hepatology Vol.62 No.6

Background & Aims: Altered expression of dual specificity phosphatase 1 (DUSP1) is common in tumors including hepatocellular carcinoma (HCC), and is predictive of tumor progression and poor prognosis. However, the tumor suppressive role of DUSP1 has yet to be clearly elucidated. Methods: The molecular mechanisms of tumor suppression that were investigated were induction of apoptosis, cell cycle inhibition, and regulation of p53. Additionally, the antitumor effect of DUSP1 was assessed using a mouse model. Associated signaling pathways in HCC cells and tissues were examined. Results: Downregulation of DUSP1 expression was significantly correlated with poor differentiation (p<0.001) and advanced HCC stage (p=0.023). DUSP1 expression resulted in HCC suppression and longer survival (p=0.0002) in a xenoplant mice model. DUSP1 inhibited p38 MAPK phosphorylation and subsequently suppressed HSP27 activation, resulting in enhanced p53 phosphorylation at sites S15, S20, and S46 in HCC cells. Enhanced p53 activation induced the expression of target genes p21 and p27, which are linked to cell cycle arrest and apoptosis. Thus, DUSP1 was potentially linked to p53 activation via the p38 MAPK/HSP27 pathway. Wild-type but not mutant p53 transcriptionally upregulated DUSP1 via its DNA-binding domain. DUSP1 and p53 might collaborate to suppress tumors in hepatocarcinogenesis via a positive regulatory loop. Conclusions: Our results revealed that disruption of a positive regulatory loop between DUSP1 and p53 promoted HCC development and progression, providing a rationale for a therapeutic agent that restores DUSP1 in HCC.

대학생의 외모에 대한 사회문화적 태도, 외모 스트레스와 섭식장애의 관계

김민지,박민정,박예진,안슬기,왕소여,이소정,이지수,정은기,하효정,김건희 이화여자대학교간호학회 2020 이화간호학회지 Vol.- No.54

Purpose: The purpose of this study is to verify the relationship between sociocultural attitudes towards appearance, appearance stress and eating disorders. Method: Sociocultural Attitudes Towards Appearance Questionnaire-4 revised which was translated to Korean measured sociocultural attitudes towards appearance and appearance stress measurement examined appearance stress. Korean Version of Eating Attitude Test-26 was used to measure eating disorders. 120 university students in a city took surveys from September 20 to October 8, 2019. Of the 120 questionnaires, 4 has insincere or incomplete responses and 116 questionnaires resulted in data. The questionnaires were investigated using statistical process of t-test, ANOVA, and Pearson's correlation coefficients using SPSS/Win 25.0 software package. Results: The average of KEAT-26 scores was 7.45 and the prevalence of eating disorders was 6.89% in the study. In eating disorders, there were significant differences according to subjective body image and diet experience. Eating disorders were positively correlated to sociocultural attitudes towards appearance (r=.21, p=.018) and appearance stress (r=.23, p=.006). Conclusion: This study verified the relationship between sociocultural attitudes towards appearance, appearance stress and eating disorders of university students, and expected to be supporting documents to develop programs for helping university students with eating disorders.

The Metabolizable Energy Value, Standardized Ileal Digestibility of Amino Acids in Soybean Meal, Soy Protein Concentrate and Fermented Soybean Meal, and the Application of These Products in Early-weaned Piglets

Zhang, H.Y.,Yi, J.Q.,Piao, X.S.,Li, P.F.,Zeng, Z.K.,Wang, D.,Liu, L.,Wang, G.Q.,Han, X. Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.5

Three experiments were conducted to evaluate the metabolizable energy (ME) value, standardized ileal digestibility (SID) of amino acids (AA) of soybean meal (SBM), soy protein concentrate (SPC) and fermented soybean meal (FSBM), and the application of these products in early-weaned piglets. In Exp. 1, four barrows with initial body weight (BW) of $14.2{\pm}1.4$ kg were used in a $4{\times}4$ Latin square design. The diet 1 contained corn as the only energy source. The other three diets replaced 25% of corn in diet 1 with one of the three soybean products, and the digestable energy (DE) and ME contents were determined by difference. In Exp. 2, four barrows (initial BW of $18.2{\pm}1.5$ kg) were fitted with ileal T-cannulas and allotted to a $4{\times}4$ Latin square design. Three cornstarch-based diets were formulated using each of the soybean products as the sole source of AA. A nitrogen-free diet was also formulated to measure endogenous losses of AA. In Exp. 3, ninety six piglets (initial BW of $5.6{\pm}0.9$ kg) weaned at $21{\pm}2$ d were blocked by weight and assigned to one of three treatments for a 21-d growth performance study. The control diet was based on corn and SBM, the two treatments' diets contained either 10% SPC or FSBM and were formulated to same SID lysine to ME ratio of 3.6 g/Mcal. The results showed that the ME content of SPC was greater than SBM (p<0.05). The SID of most AA in SPC was greater than the SID of AA in SBM (p<0.05). For the essential AA, the SID of histidine, isoleucine, leucine, lysine and threonine in FSBM were greater than in SBM (p<0.05). Even though they were fed same SID lysine to ME ratio of 3.6 g/Mcal diets, pigs fed SPC and FSBM diets had greater weight gain, G:F (p<0.05) and better fecal score (p<0.05) than pigs fed SBM diet. In conclusion, SPC showed a higher ME content and SID of AA than the SBM. SID of some essential AA in FSBM was higher than SBM and was similar with SPC. But the lower antigenic proteins and anti-nutritional factors content in SPC and FSBM may be the main factors affecting the performance of early-weaned piglets rather than the increased ME content and SID of AA.