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Searching For Transcription Factors Involved In Ammonium Assimilation and Root Growth in Rice Plants
Ryza A. Priatama,Vikranth Kumar,Jin-hee Jeong,Chang-deok Han 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
Nitrogen in rice paddy soils and utilized as the major source for N-assimilation in rice crops. In roots, transcriptional activities of ammonium uptake and assimilation genes are highly sensitive to the availability of exogenous ammonium. However, little is known about the transcription factor genes that regulated by ammonium supply and its role to roots and plant developments. To study the transcription factor genes that involved in Ammonium response, two weeks old rice seedlings treated using Ammonium from 0 to 3 hours. Total RNA collected from each sample and samples were prepared for Agilent 8x60K microarray system. Based on the microarray data, we select transcription factor genes that highly affected by ammonium and selected knock out mutant candidates that used for phenotype screening.
Liu, Jing Miao,Park, Soon Ju,Huang, Jin,Lee, Eun Jin,Xuan, Yuan Hu,Je, Byoung Il,Kumar, Vikranth,Priatama, Ryza A.,Raj K, Vimal,Kim, Sung Hoon,Min, Myung Ki,Cho, Jun Hyeon,Kim, Tae Ho,Chandran, Anil K Oxford University Press 2016 Journal of experimental botany Vol.67 No.6
<P>Lamina inclination is a key agronomical character that determines plant architecture and is sensitive to auxin and brassinosteroids (BRs). <I>Loose Plant Architecture1</I> (<I>LPA1</I>) in rice (<I>Oryza sativa</I>) and its Arabidopsis homologues (<I>SGR5/AtIDD15</I>) have been reported to control plant architecture and auxin homeostasis. This study explores the role of <I>LPA1</I> in determining lamina inclination in rice. <I>LPA1</I> acts as a positive regulator to suppress lamina bending. Genetic and biochemical data indicate that <I>LPA1</I> suppresses the auxin signalling that interacts with C-22-hydroxylated and 6-deoxo BRs, which regulates lamina inclination independently of <I>OsBRI1</I>. Mutant <I>lpa1</I> plants are hypersensitive to indole-3-acetic acid (IAA) during the lamina inclination response, which is suppressed by the brassinazole (Brz) inhibitor of C-22 hydroxylase involved in BR synthesis. A strong synergic effect is detected between <I>lpa1</I> and <I>d2</I> (the defective mutant for catalysis of C-23-hydroxylated BRs) during IAA-mediated lamina inclination. No significant interaction between <I>LPA1</I> and <I>OsBRI1</I> was identified. The <I>lpa1</I> mutant is sensitive to C-22-hydroxylated and 6-deoxo BRs in the <I>d61-1</I> (rice <I>BRI1</I> mutant) background. We present evidence verifying that two independent pathways function via either BRs or <I>BRI1</I> to determine IAA-mediated lamina inclination in rice. RNA sequencing analysis and qRT-PCR indicate that <I>LPA1</I> influences the expression of three <I>OsPIN</I> genes (<I>OsPIN1a</I>, <I>OsPIN1c</I> and <I>OsPIN3a</I>), which suggests that auxin flux might be an important factor in <I>LPA1</I>-mediated lamina inclination in rice.</P>
Study on Phenotypes and Agronomical utility of a Rice GT1 (grassy tillers 1, OsGT1) Homologue
Vikranth Kumar,Yuan Hu Xuan,Byoung Il Je,Soon Ju Park,Jin Huang,Jing Miao Liu,Ryza A. Priatama,Vimal Raj K,Sung Hoon Kim,Jin-hee Jeong,Chang-deok Han 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
Enhancing yield has been a major challenge of agriculture. In rice, tiller number is one of the important biomass and yield components. A maize mutant grassy tillers1 (gt1) increases lateral branches in maize. The GT1 gene encodes a class I homeodomain leucine zipper (HD-Zip) protein. In maize, the gt1 expression is induced by shading and is dependent on the activity of teosinte branched1 (tb1), a major domestication locus controlling tillering and lateral branching. To estimate the biological role and agricultural utility of gt1 in rice, rice homologue (OsGT1) has been isolated and its overexpressors and RNAi lines were generated. Field data showed that OsGT1 overexpressors reduced tillers and panicles while RNAi lines increased them, compared to wild type. Shade signal is an important factor in determining lateral branching. To understand the relationship between OsGT1 and shade avoidance, plants have been grown under 50% shading in the field. Also, double genetic combinations with phytochrome mutants (phyA, B, and C) are being examining for tillering phenotype. These ongoing researches will provide insights in determining the action of OsGT1 on branching and shade avoidance in rice.
Kumar Vikranth,Kim Sung Hoon,ADNAN MOCH ROSYADI,허정,정진희,Priatama Ryza A.,이증주,김철민,제병일,박순주,Xuan Yuan Hu,한창덕 한국식물학회 2021 Journal of Plant Biology Vol.64 No.5
Tillering is one of the most important determinants of biomass and yield in rice (Oryza sativa L.). The capacity of plants to develop tillers from primordial meristems or buds is determined not only by the genotype but also by environmental cues. Here, we characterized the function of rice grassy tiller1 (OsGT1) and its interaction with other genetic and biological factors involved in tiller bud outgrowth in rice by generating OsGT1 RNA interference (RNAi) and overexpression (OX) lines. The tiller number was increased in OsGT1-RNAi mutants but strongly suppressed in OsGT1-OX lines. Expression analysis of OsGT1 in rice phyB mutants and in genotypes carrying various genetic combinations of GT1 RNAi and phyB demonstrated that OsGT1 is not involved in phyB-mediated suppression of tiller development in rice. Expression analysis of fine culm1 (fc1), a rice tb1 homolog, and molecular assays demonstrated that FC1 enhances the expression of OsGT1 by directly binding to its promoter. Comparison of the transcriptomic profiles of fc1 and OsGT1-RNAi mutants revealed differentially expressed genes (DEGs) common to both genotypes. Finally, analysis of tillering phenotypes of OX and RNAi seedlings treated with various phytohormones implied a possible role of OsGT1 in strigolactone-mediated tiller outgrowth. Overall, this study enhances our understanding of the diverse mechanisms of tiller development in grasses.
Medicinal metabolites with common biosynthetic pathways in Solanum nigrum
Anitha Jabamalairaj,Ryza A. Priatama,허정,박순주 한국식물생명공학회 2019 Plant biotechnology reports Vol.13 No.4
Solanum nigrum is a medicinal plant belonging to the Solanaceae family. It possesses various therapeutic properties such as anticancer, antioxidant, hepatoprotective, antiulcer, antiinflammatory, antihyperlipidemic, antidiabetic, antibacterial, and antiseizure properties. Numerous secondary metabolites have been isolated from S. nigrum, including (+) syringaresinol (II), (+)-medioresinol (III), scopoletin (IV), tetracosanoic acid (V), and beta-sitosterol (IV). They also contain a variety of toxic secondary metabolites such as alpha-Solanine and alpha-Chaconine, which are steroidal glycoalkaloids. Here, we showed the potential of CRISPR/Cas9 technology to improve and enhance the plant architecture, secondary metabolites, and therapeutic applications in eliminating toxic compounds, especially solanine and chaconine, in S. nigrum.
Vikranth Kumar,Sung Hoon Kim,Ryza A. Priatama,Jin Hee Jeong,Moch Rosyadi Adnan,Bernet Agung Saputra,Chul Min Kim,Byoung Il Je,Soon Ju Park,Ki-Hong Jung,Kyung Min Kim,Yuan Hu Xuan,Chang‑deok Han 한국식물학회 2020 Journal of Plant Biology Vol.63 No.5
The AMT1 family comprises major ammonium transporters in rice roots. In this study, we utilized AMT1 RNAi mutants (amt1) to explore how AMT1 affects NH4+- and NO3–-mediated morphological development and NH4+-responsive gene expression in roots. In the presence of NH4+, amt1 showed inhibition of NO3–- dependent lateral root development. The inhibitory action of NH4+ on lateral root growth was independent of the NO3– concentrations supplied to amt1 roots. The results of split root assays indicated that NH4+ exerts systemic action in inhibiting NO3–-dependent lateral root development in amt1. Further study with NAA and NOA, a potent auxin flux inhibitor, suggested that perturbation of membrane dynamics might not be the primary cause of the inhibitory action of NH4+ on NO3–-mediated lateral root growth in amt1 mutants. RNA-seq analysis of NH4+-responsive genes showed that approximately half of DEGs observed in wild-type roots were not detected in the DEGs of amt1 roots. Gene ontology enrichment analysis suggested that the expression of specific functional gene groups were affected by amt1 during the early response to NH4+. Auxin-responsive gene expression and root gravity responses were altered in amt1. This study demonstrated that AMT1 affects the interactions not only between ammonium and nitrate in lateral root growth but also between auxin and NH4+ in rice roots.