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ASTM F813-07 의료용디바이스를 위한 생체재료의 직접촉 세포배양측정에 대한 표준지침서
김순희 ( Soon Hee Kim ),백미옥 ( Mi Ock Baek ),소정원 ( Jung Won So ),노혜원 ( Hye Won Roh ),이나리 ( Na Ri Lee ),김문석 ( Moon Suk Kim ),유규하 ( Gyu Ha Ryu2,),조양하 ( Yang Ha Cho ),이승진 ( Seung Jin Lee ),강길선 ( Gilson Khang 한국조직공학·재생의학회 2007 조직공학과 재생의학 Vol.4 No.4
We provided standard practice for direct contact cell culture evaluation of materials for medical devices in this report. This practice introduced a reference method of direct contact cell culture testing. This method of direct contact cell culture may be used in the construction of medical materials and devices for test the cytotoxic potential of materials. Researcher for medical devices could use this practice to compare other cytotoxicity tests or directly to evaluate materials. Cell or materials could be sometimes modified depending on use in offered extend of this practice.
ASTM F2027 조직공학적 의료용제품(TEMPs)을 위한 기질의 특성결정 및 테스트를 위한 표준 지침서
백미옥 ( Mi Ock Baek ),소정원 ( Jung Won So ),김순희 ( Soon Hee Kim ),노혜원 ( Hye Won Roh ),이나리 ( Na Ri Lee ),김문석 ( Moon Suk Kim ),유규하 ( Gyu Ha Ryu2 ),조양하 ( Yang Ha Cho ),이승진 ( Seung Jin Lee ),강길선 ( Gilson Khang 한국조직공학·재생의학회 2007 조직공학과 재생의학 Vol.4 No.4
Tissue engineering medical products are those protocols and products developed for use in the human body as biological substitutes to restore, maintain, or improve tissue function. The purpose of this standard is to locate relevant existing guideline and test methods and to provide guidance for interim use of materials for which a standard does not exist. The standard may be use as guideline in tissue engineered research.
Cell Cycle Regulators during Human Atrial Development
(Won Ho Kim),(Chan Uhng Joo),(Ja Hong Ku),(Chul Hee Ryu),(Keum Nim Koh),(Gou Young Koh),(Jae Ki Ko) 대한내과학회 1998 The Korean Journal of Internal Medicine Vol.13 No.2
N/A Objectives : The molecular mechanisms that regulate cardiomyocyte cell cycle and terminal differentiation in humans remain largely unknown. To determine which cyclins, cyclin dependent kinases (CDKs) and cyclin kinase inhibitors (CKIs) are important for cardiomyocyte proliferation, we have examined protein levels of cyclins, CDKs and CKIs during normal atrial development in humans. Methods : Atrial tissues were obtained in the fetus from inevitable abortion and in the adult during surgery, Cyclin and CDK proteins were determined by Western blot analysis, CDK activities were determined by phosphorylation amount using specific substrate. Results : Most cyclins CDKs were high during the fetal period and their levels decreased at different rates during the adult period. While the protein levels of cyclin D1, cyclin D3, CDK4, CDK6 and CDK2 were still detectable in adult atria, the protein levels of cyclin E, cyclin A, cyclin B, cdc2 and PCNA were not detectable. Interestingly, p27KIP1 protein increased markedly in the adult period, while p21CIP1 protein in atria was detectable only in the fetal period. While the activities of CDK6, CDK2 and cdc2 decreased markedly, the activity of CDK4 did not change from the fetal period to the adult period. Conclusion : These findings indicate that marked reduction of protein levels and activities of cyclins and CDKs, and marked induction of p27KIP1 in atria, are associated with the withdrawal of cardiac cell cycle in adult humans.
( Won-mook Choi ),( Jonggi Choi ),( Danbi Lee ),( Ju Hyun Shim ),( Kang Mo Kim ),( Young-suk Lim ),( Han Chu Lee ),( Changhoon Yoo ),( Sook Ryun Park ),( Min-hee Ryu ),( Baek-yeol Ryoo ) 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1
Aims: Nivolumab showed durable response and safety in patients with hepatocellular carcinoma (HCC) in the previous trials. However, real-world data of nivolumab in HCC patients, especially those with Child-Pugh class B, is lacking. We aimed to investigate the efficacy and safety of nivolumab in a real- world cohort of patients with advanced HCC. Methods: This study retrospectively evaluated 203 patients with HCC who were treated with nivolumab between July 2017 to February 2019. Radiologic evaluation was based on mRECIST. Survival outcomes were estimated by Kaplan-Meier method and Cox proportional hazard model. Logistic regression model was used to identify the predictive factors of treatment response. Results: Of 203 patients, 132 patients were within Child-Pugh class A and 71 patients were within Child-Pugh class B. Objective response rate was lower in patients with Child-Pugh class B than A (2.8% vs. 15.9%; P=0.010 by unweighted analysis and P=0.034 by weighted analysis) and Child-Pugh class was an independent predictor for objective response (Odds ratio, 0.21; 95% confidence interval; 0.05-0.93; P=0.040). Median overall survival was shorter in Child-Pugh B patients (11.3 vs. 42.9 weeks; P<0.001 by both unweighted and weighted analyses). However, other efficacy outcomes including disease control rate, time to progression, and progression-free survival were comparable between Child-Pugh A and B patients by unadjusted, adjusted, matched, and weighted analyses. There was no significant difference in terms of safety between Child-Pugh A and B patients. Conclusions: Given the limited treatment options for advanced HCC in Child-Pugh B patients, nivolumab may be a viable option despite lower response in these patients. Further studies are needed in this patient population.
Influence of Transmucosal Designs of Dental Implant on Tissue Regeneration in Beagle Dogs
( Won Hee Kim ),( Young Ku Heo ),( Chang Mo Jeong ),( Dong Woo Cho ),( Jae Jun Ryu ),( Jung Bo Huh ) 한국조직공학·재생의학회 2013 조직공학과 재생의학 Vol.10 No.1
This study was conducted to evaluate the influence of concave machined and concave microgrooved profiles of the transmucosal portion of implants on early peri-implant tissue regeneration. We assessed implants with 3 different transmucosal profiles: the bevel group that had only a bevel in the transmucosal part, bioseal group and bioseal/ groove group that had a micro-groove in the S-shaped portion of the implant. A total of 36 implants (12 of each type) were placed in the edentulous mandibular ridges of 6 beagle dogs. We used radiographs to investigate the mesio-distal change of the marginal bone. Bucco-lingual bone resorption and soft tissue reactions were evaluated histologically. Radiographic and histological analysis did not show any difference in mesio-distal or bucco-lingual marginal bone resorption among any of the 3 groups (p > 0.05). The bioseal and bioseal/groove groups had more rigid connective tissue attachment than that in the bevel group. However, the bevel group had significantly long junctional epithelium attachment and the bioseal and bioseal/groove groups had long connective tissue attachment (p < 0.05). Implants with S-shaped concavity of the transmucosal portion resulted in a firmer connective tissue barrier, and thus, a better soft tissue regeneration, than implants with straight bevel.
Microbial Contamination Levels of Strawberries at Domestic Farms of South Korea
Won-Il Kim,A-Ra Jo,Se-Ri Kim,Song Hee Ryu,Ki-Woong Nam,Yohan Yoon,Deok-Hoon Yoon,So-Yong Oh,Myeong Hyeon Nam,Jae-Gee Ryu,Hwang-Yong Kim 한국토양비료학회 2014 한국토양비료학회지 Vol.47 No.6
Foodborne illness due to the consumption of contaminated raw strawberries is a continuing food safety concern. This study investigated and evaluated contamination levels of bacteria on strawberries at farms stage to evaluate potential hazards associated with fresh strawberries. A total of 315 samples, 105 samples from 5 sampling sites (A to E) of 21 farms and 210 samples from 1 sampling site of 6 farms, was collected every month for four months and analyzed to enumerate aerobic bacterial counts, Coliforms/E. coli, Bacillus cereus and Staphylococcus aureus. In addition, the prevalence study of five pathogens (S. aureus, E. coli, E. coli O157:H7, Salmonella spp. and Listeria monocytogenes) was performed on each sample. Aerobic bacterial counts ranged from 0.48 to 6.36 Log CFU/g, with the highest bacterial cell counts recorded for D and E sites. Coliforms were detected in 71 samples (22.5%) with a minimum of 0.48 cfu/g and a maximum of more than 4 Log CFU/g. B. cereus was detected in 98 samples (31.1%) among total samples analyzed. S. aureus was detected in 2 samples with a minimum of 0.48 Log CFU/g and a maximum of 1.38 Log CFU/g. E. coli, E. coli O157:H7, Salmonella spp. and L. monocytogenes were not isolated from any of the samples. The microbial contamination levels of strawberries determined in this study may be used as the fundamental data for microbiological risk assessment.
Ryu Yeon Hee,Moon Suk-Ho,Kim Ki Joo,Jun Young Joon,Oh Deuk-Young,Kim Sang‐Heon,Rhie Jong-Won 한국조직공학과 재생의학회 2021 조직공학과 재생의학 Vol.18 No.3
Background: Angiogenesis and vasculogenesis are essential processes for successful tissue regeneration in tissue engineering and regenerative medicine. The adipose-derived stromal vascular fraction (SVF) is not only a source of adipose stem cells (ASC) but also a suitable source of microvascular endothelial cells because it is a rich capillary network. So, we propose a new hypothesis for isolating adipose-derived human microvascular endothelial cells (HMVEC-A) from the SVF and developed a dual isolation system that isolates two cell types from one tissue. Method: To isolate HMVEC-A, we analyzed the supernatant discarded when ASC is isolated from the adipose-derived SVF. Based on this analysis, we assumed that the SVF adherent to the bottom of the culture plate was divided into two fractions: the stromal fraction as the ASC-rich fraction, and the vascular fraction (VF) as the endothelial cells-rich fraction floating in the culture supernatant. VF isolation was optimized and the efficiency was compared, and the endothelial cells characteristics of HMVEC-A were confirmed by flow cytometric analysis, immunocytochemistry (ICC), a DiI-acetylated low-density lipoprotein (DiI-Ac-LDL) uptake, and in vitro tube formation assay. Results: Consistent with the hypothesis, we found a large population of HMVEC-A in the VF and isolated these HMVEC-A by our isolation method. Additionally, this method had higher yields and shorter doubling times than other endothelial cells isolation methods and showed typical morphological and phenotypic characteristics of endothelial cells. Conclusion: Cells obtained by the method according to our hypothesis can be applied as a useful source for studies such as tissue-to-tissue networks, angiogenesis and tissue regeneration, patient-specific cell therapy, and organoid chips. Background: Angiogenesis and vasculogenesis are essential processes for successful tissue regeneration in tissue engineering and regenerative medicine. The adipose-derived stromal vascular fraction (SVF) is not only a source of adipose stem cells (ASC) but also a suitable source of microvascular endothelial cells because it is a rich capillary network. So, we propose a new hypothesis for isolating adipose-derived human microvascular endothelial cells (HMVEC-A) from the SVF and developed a dual isolation system that isolates two cell types from one tissue. Method: To isolate HMVEC-A, we analyzed the supernatant discarded when ASC is isolated from the adipose-derived SVF. Based on this analysis, we assumed that the SVF adherent to the bottom of the culture plate was divided into two fractions: the stromal fraction as the ASC-rich fraction, and the vascular fraction (VF) as the endothelial cells-rich fraction floating in the culture supernatant. VF isolation was optimized and the efficiency was compared, and the endothelial cells characteristics of HMVEC-A were confirmed by flow cytometric analysis, immunocytochemistry (ICC), a DiI-acetylated low-density lipoprotein (DiI-Ac-LDL) uptake, and in vitro tube formation assay. Results: Consistent with the hypothesis, we found a large population of HMVEC-A in the VF and isolated these HMVEC-A by our isolation method. Additionally, this method had higher yields and shorter doubling times than other endothelial cells isolation methods and showed typical morphological and phenotypic characteristics of endothelial cells. Conclusion: Cells obtained by the method according to our hypothesis can be applied as a useful source for studies such as tissue-to-tissue networks, angiogenesis and tissue regeneration, patient-specific cell therapy, and organoid chips.
( Won-mook Choi ),( Jonggi Choi ),( Danbi Lee ),( Ju Hyun Shim ),( Young-suk Lim ),( Han Chu Lee ),( Young-hwa Chung ),( Young-sang Lee ),( Sook Ryun Park ),( Min-hee Ryu ),( Baek-yeol Ryoo ),( So Jun 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1
Aims: Regorafenib and nivolumab are drugs approved for second-line treatment of patients with hepatocellular carcinoma (HCC) after sorafenib failure. However, the effectiveness of regorafenib and nivolumab following sorafenib has not been directly compared. Methods: This study retrospectively evaluated 373 patients with HCC who were treated with regorafenib (n=223) or nivolumab (n=150) after sorafenib failure between July 2017 and February 2019. Results: Progression-free survival (PFS; hazard ratio [HR], 0.85; 95% confidence interval [CI], 0.69-1.06; P=0.150), time to progression (TTP; HR, 0.95; 95% CI, 0.77-1.19; P=0.680), and overall survival (OS; HR, 0.83; 95% CI, 0.64-1.07; P=0.154) did not differ significantly between groups of patients treated with regorafenib and nivolumab, findings consistently observed by multivariable-adjusted, propensity score-matched, and inverse probability treatment weighting (IPTW) analyses. However, the objective response rate was significantly higher in the nivolumab than in the regorafenib group (13.3% vs, 4.0%; P=0.002). When the effectiveness of regorafenib and nivolumab was compared in non-progressors to treatment, defined as patients who achieved complete response, partial response, or stable disease after first response evaluation, PFS (HR, 0.50; 95% CI, 0.33-0.75; P=0.001), TTP (HR, 0.48; 95% CI, 0.31-0.73; P<0.001), and OS (HR, 0.51; 95% CI, 0.31-0.87; P=0.013) were significantly longer in the 59 non-progressors to nivolumab than in the 104 non-progressors to regorafenib, findings also observed by multivariable-adjusted and IPTW analyses. Conclusions: Survival outcomes in patients treated with regorafenib and nivolumab after sorafenib failure did not differ significantly. However, nivolumab may be more effective than regorafenib in non-progressors.