Synergistic Effects of Arbuscular Mycorrhizal Fungi and Plant Growth Promoting Rhizobacteria for Sustainable Agricultural Production

Krishnamoorthy Ramasamy,Manoharan Melvin Joe,김기윤,이선미,Charlotte Shagol,Anandham Rangasamy1,정종배,Md. Rashedul Islam,사동민 한국토양비료학회 2011 한국토양비료학회지 Vol.44 No.4

Soil microorganisms play a major role in improving soil fertility and plant health. Symbiotic arbuscular mycorrhizal fungi (AMF) form a key component of the soil microbial populations. AMF form a mutualistic association with the host plant and exert a positive influence on its growth and nutrient uptake. The establishment of mycorrhizal symbioses with the host plant can positively be influenced by plant growth promoting rhizobacteria through various mechanisms such as increased spore germination and hyphal permeability in plant roots. Though there are evidences that combined interactions between AMF and PGPR can promote the plant growth however mechanisms of these interactions are poorly understood. Better understanding of the interactions between AMF and other microorganisms is necessary for maintaining soil fertility and enhancing crop production. This paper reviews current knowledge concerning the interactions between AMF and PGPR with plants and discusses on enhanced nutrient availability, biocontrol, abiotic stress tolerance and phytoremediation in sustainable agriculture.

Isolation of Sulfur Oxidizing Bacteria from Different Ecological Niches

Rangasamy Anandham,Ragupathy Sridar,Periyakaruppan Nalayini,Munusamy Madhaiyan,Pandiyan Indira Gandhi,Kwan-Ho Choi(최관호),Tong Min Sa(사동민) 한국토양비료학회 2005 한국토양비료학회지 Vol.38 No.4

Paraherbaspirillum soli gen. nov., sp. nov. Isolated from Soil

Rangasamy Anandham,김수진,문지영,원항연,권순우 한국미생물학회 2013 The journal of microbiology Vol.51 No.2

A bacterial strain, designated JS5-2T, was isolated from soil collected from Jeju Island, Republic of Korea. The cells of the strain were Gram-negative, nonspore forming, catalaseand oxidase-positive, aerobic, nonmotile and rod-shaped. Strain JS5-2T exhibited 96.2–97.2, 95.1–96.3, and 95.4–95.8%16S rRNA gene sequence similarities to the genera Herbaspirillum,Oxalicibacterium, and Herminiimonas, respectively. The highest sequence similarities were with Herbaspirillum autotrophicum IAM 14942T (97.2%) and Herbaspirillum frisingense GSF30T (97.1%). The major fatty acids of strain JS5-2T were C16:0(35.0%), C17:0 cyclo (19.9%), C18:1 ω7c (11.4%),and summed feature 3 (C16:1 ω7c/C15:0 iso 2-OH) (15.2%),and the major polar lipids of strain JS5-2T were diphosphatidylglycerol and an unknown aminophospholipid. The strain contained Q-8 as the predominant ubiquinone. DNA-DNA relatedness values between strain JS5-2T and H. autotrophicum IAM 14942T, and H. frisingense GSF30T were 32 and 35%, respectively. The DNA G+C content of strain JS5-2T was 59.0 mol%. On the basis of phenotypic, genotypic, and physiological evidence, strain JS5-2T represents a novel species of a new genus, for which the name Paraherbaspirillum soli gen. nov., sp. nov. is proposed. The type strain JS5-2T (=KACC 12633T =NBRC 106496T) is proposed.

Microbacterium suwonense sp. nov., Isolated from Cow Dung

Rangasamy Anandham,Tomohiko Tamura,Moriyuki Hamada,원항연,Soo-Jin Kim,Yi-Seul Kim,Ken-ichiro Suzuki,권순우 한국미생물학회 2011 The journal of microbiology Vol.49 No.5

An actinomycete strain, designated M1T8B9^T, was isolated from cow dung in Suwon, Republic of Korea. The isolate was a Gram-positive, nonmotile, and non-spore-forming bacterium. Phylogenetic evaluation based on 16S rRNA gene sequence similarity showed that this isolate belongs to the genus Microbacterium, with its closest neighbors being Microbacterium soli DCY17^T (98.2%) and Microbacterium esteraromaticum DSM 8609^T (98.0%). The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, and one unknown glycolipid. Strain M1T8B9^T contained the major fatty acids C_(15:0) anteiso, C_(16:0) iso, C_(17:0) anteiso, and C_(15:0) iso, and the cell-wall peptidoglycan was of type B2β. According to DNA-DNA hybridization studies, strain M1T8B9^T showed 42% and 26% relatedness with M. soli DCY17^T and M. esteraromaticum DSM 8609^T, respectively. On the basis of the data presented, strain M1T8B9^T is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium suwonense sp. nov. is proposed. The type strain is M1T8B9^T (=KACC 14058^T =NBRC 106310^T).

Mitochondria and DNA Targeting of 5,10,15,20-Tetrakis(7-sulfonatobenzo[<i>b</i>]thiophene) Porphyrin-Induced Photodynamic Therapy via Intrinsic and Extrinsic Apoptotic Cell Death

Rangasamy, Sabarinathan,Ju, Hee,Um, Soohyun,Oh, Dong-Chan,Song, Joon Myong American Chemical Society 2015 Journal of medicinal chemistry Vol.58 No.17

<P>Photodynamic therapy (PDT) selectively targets subcellular organelles and promises an excellent therapeutic strategy for cancer treatment. Here, we report the synthesis of a new water-soluble photosensitizer, 5,10,15,20-tetrakis (7-sulfonatobenzo[<I>b</I>]thiophene) porphyrin (SBTP). Rational design of the porphyrinic molecule containing benzo[<I>b</I>]thiophene moiety at the <I>meso</I>-position led to selective accumulation in both mitochondria and nucleus of MCF-7 cells. This multitarget ability of SBTP can cause damage to mitochondria as well as DNA simultaneously. FACS analysis showed rapid cellular uptake of SBTP. High-content cell-based assay was executed to concurrently monitor increase of cytosolic Ca<SUP>2+</SUP> levels, mitochondrial permeability transition (MPT), and caspase-3/7/8 activation in MCF-7 cells under the pathological condition caused by PDT action of SBTP. The study of cell death dynamics showed that PDT action of SBTP caused an increase in the MPT followed by an increase in cytosolic Ca<SUP>2+</SUP> level. The localization of SBTP in the mitochondria activated the intrinsic apoptotic pathway. Additionally, localization of SBTP in the nucleus led to DNA damage in MCF-7 cells. The DNA fragmentation that occurred by PDT action of SBTP was thought to be responsible for extrinsic apoptosis of MCF-7 cells. SBTP demonstrated effective PDT activity of 5 μM IC<SUB>50</SUB> value to MCF-7 cells by bitargeting mitochondria and DNA.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jmcmar/2015/jmcmar.2015.58.issue-17/acs.jmedchem.5b01095/production/images/medium/jm-2015-01095g_0011.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jm5b01095'>ACS Electronic Supporting Info</A></P>

Multi layered Si-CuO quantum dots wrapped by graphene for high-performance anode material in lithium-ion battery

Rangasamy, B.,Hwang, J.Y.,Choi, W. Pergamon Press ; Elsevier Science Ltd 2014 Carbon Vol.77 No.-

Various approaches to improve the efficiency of Lithium ion batteries (LiB) by using Si have been suggested because Si has the highest known lithium capacity. Although Si is more than ten times higher capacity than existing graphite anodes, Si anodes have limited applications due to its high volume change during cycling. Here we demonstrated graphene/Si-CuO quantum dots (Gr/Si-CuO QD) layered structure as an efficient LiB anode which prevents large volume expansion of Si due to the presence of CuO-Cu<SUB>3</SUB>Si. By Electrophoresis Deposition technique, the multi-layer of graphene and Si-CuO QD has been successfully fabricated followed by annealing process to form Cu<SUB>3</SUB>Si interlayer as confirmed by High Resolution Transmission Electron Microscope-Energy Dispersive Spectroscopy, X-ray diffraction and Raman analyses. The annealed Gr/Si-CuO QD exhibit the initial gravimetric specific capacity of 2869mAhg<SUP>-1</SUP> which is five times higher than that of annealed graphene at 0.5C. After 100 cycles at 1C rate the capacity retains ~71% and the excellent rate capability even at high C rate reveals controlled volume expansion owing to the multi layered architecture and the Cu<SUB>3</SUB>Si inter layer. The layered structure of Gr/Si-CuO QD electrode could be applied in next generation micro power sources.

New topological operators over intuitionistic fuzzy sets

P. Rangasamy,P. V. Vassilev,K. T. Atanassov 장전수학회 2009 Advanced Studies in Contemporary Mathematics Vol.18 No.1

Four new intutionistic fuzzy topological operators are constructed. Their basic properties are studied and their geometrical inperptretations are shown.

Acinetobacter brisouii sp. nov., Isolated from a Wetland in Korea

Anandham, Rangasamy,Weon, Hang-Yeon,Kim, Soo-Jin,Kim, Yi-Seul,Kim, Byung-Yong,Kwon, Soon-Wo 한국미생물학회 2010 The journal of microbiology Vol.48 No.1

A bacterial strain $5YN5-8^T$ was isolated from peat layer on Yongneup in Korea. Cells of strain $5YN5-8^T$ were strictly aerobic, Gram-negative, coccobacilli, non-spore forming, and non-motile. The isolate exhibited optimal growth at $28^{\circ}C$, pH 7.0, and 0-1% NaCl. Results of 16S rRNA gene sequence analyses indicated a close relationship of this isolate to Acinetobacter calcoaceticus (97.8% similarity for strain DSM $30006^T$). It also exhibited 94.4-97.8% 16S rRNA gene sequence similarities to the validly published Acinetobacter species. The value for DNA-DNA hybridization between strain $5YN5-8^T$ and other members of the genus Acinetobacter ranged from 16 to 28%. Predominant cellular fatty acids were $C_{18:1}$ ${\omega}9c$, summed feature 4 containing $C_{15:0}$ iso 2-OH and/or $C_{16:1}$ ${\omega}7c$, and $C_{16:0}$. The DNA G+C content was 43.9 mol%. Phylogenetic, phenotypic, and chemotaxonomic data accumulated in this study revealed that the isolate could be classified in a novel species of the genus Acinetobacter. The name Acinetobacter brisouii sp. nov. is proposed for the novel species, with $5YN5-8^T$ (=KACC $11602^T$ =DSM $18516^T$) as the type strain.

Hockey activity recognition using pre-trained deep learning model

Keerthana Rangasamy,Muhammad Amir Asari,Nur Azmina Rahmad,Nurul Fathiah Ghazali 한국통신학회 2020 ICT Express Vol.6 No.3

Activity recognition in sports is often complex task resulting from the rapid dynamic interaction within players. In this paper, pre-trained VGG-16, deep learning based hockey activity recognition model has been proposed. Own hockey dataset consisting of four main activity includes free hit, goal, penalty corner and long corner was constructed as there are no existing field hockey datasets available. Experimental results indicate that the pre-trained deep learning model generates comparative results on this challenging dataset by tweaking the hyperparameters of this pre-trained model.

Rhodocytophaga aerolata gen. nov., sp. nov., a new member of the family Cytophagaceae isolated from air

Anandham, Rangasamy,Weon, Hang-Yeon,Kim, Soo-Jin,Kim, Yi-Seul,Kwon, Soon-Wo Microbiology Society 2010 International journal of systematic and evolutiona Vol.60 No.7

<P>A strictly aerobic, Gram-staining-negative, oxidase- and catalase-positive, non-motile, rod-shaped bacterium, designated strain 5416T-29<SUP>T</SUP>, was isolated from air and was characterized by using a polyphasic approach. Colonies were reddish pink and circular with entire margins. Flexirubin-type pigments were absent. The strain formed a distinct phylogenetic lineage within the family <I>Cytophagaceae</I> of the phylum <I>Bacteroidetes</I>. Strain 5416T-29<SUP>T</SUP> did not show more than 88 % 16S rRNA gene sequence similarity to the type strain of any recognized species. The major cellular fatty acids were C16 : 1<I>ω</I>5<I>c</I>, iso-C17 : 0 3-OH and iso-C15 : 0. The polar lipids were phosphatidylethanolamine, one unknown amino lipid and several unknown polar lipids. Menaquinone-7 (MK-7) was the major respiratory quinone. The G+C content of the DNA of strain 5416T-29<SUP>T</SUP> was 45.5 mol%. Results of phenotypic and phylogenetic analyses clearly indicate that strain 5416T-29<SUP>T</SUP> represents a novel species of a new genus in the family <I>Cytophagaceae</I>, for which the name <I>Rhodocytophaga aerolata</I> gen. nov., sp. nov. is proposed. The type strain of <I>Rhodocytophaga aerolata</I> is 5416T-29<SUP>T</SUP> (=KACC 12507<SUP>T</SUP> =DSM 22190<SUP>T</SUP>).</P>