Accuracy of Ultrasonography in Early Pregnancy Diagnosis in Doe

Singh, N.S.,Gawande, P.G.,Mishra, O.P.,Nema, R.K.,Mishra, U.K.,Singh, Mohan Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.6

The present study was undertaken to evaluate the accuracy of ultrasonography in early pregnancy diagnosis in goats. Ultrasonographic scanning with real time B-mode ultrasound machine having 5 MHz linear array transducer was performed on gravid uterus (n=24) obtained from slaughterhouse (Group I). Crown rump length (CRL) measured by ultrasound was found significantly different (p<0.05) with actual CRL measured after dissection in early pregnancy. However, age predicted by ultrasound through the measurement of CRL was found highly correlated (r=0.92) with age measured after dissection through CRL and the weight of fetus. Ages predicted by ultrasound through the measurement of trunk diameter (TD) and uterine diameter (UD) and ages measured after dissection were found highly and equally correlated (r=0.98) and did not differ significantly. Data from six does synchronized (Group II) with PGF2$\alpha$ (Estrumate) at 11 days apart were collected through ultrasound from 17 to 42 days post breding. The correlation between CRL and gestational age was high (r=0.97) in day 30 to 42 post breeding. A high coefficient of correlation (r=0.98) was also observed between predicated age by ultrasound and actual age calculated after kidding. The correlation between CRL and gestational age by the formula Y=(a+bX) i.e. Y=24.42+0.39 X where Y=gestational age and X=CRL, was recorded very high (r=0.99). Accuracy of ultrasonography was lowest on day 17 to 19 (66%) and reached 100% on day 34. Data from 30 does (group III) randomly subjected to only one time ultrasounds scanning to assess the accuracy of pregnancy diagnosis were also obtained. Ages predicted by TD and UD measurements were observed to be non-significantly different with actual age obtained after kidding and correlation between ages predicted by TD and UD measurement with actual age after kidding was found equally and highly correlated (r=0.98). The operator's accuracy in the whole experiment including all three groups was found to be 92%. The sensitivity was 93% and specificity was 86%. From the present study, it was observed that CRL was the most reliable parameter to find out gestational age in early pregnancy and the new formula derived was found very accurate to find out gestational age. TD and UD were also found to be equally reliable parameter to find out gestational age in mid and late stage of pregnancy through ultrasonography. It was concluded that ultrasonography by real time B mode with 5 MHz transrectal transducer was found to be reliable, safe and accurate and practicable means in diagnosing early pregnancy diagnosis as early as 25 days post breeding.

Calcium - Boron Interaction in Exopolysaccharide Production by the Cyanobacterium , Nostoc spongiae forme

(Singh),(Neetu),(R . K . Asthana),(S . P . Singh) 한국미생물생명공학회 2000 Journal of microbiology and biotechnology Vol.10 No.3

N/A N/A

Production and Optimization of a Commercially Viable Alkaline Protease from a Haloalkaliphilic Bacterium

R. H. Joshi,M. S. Dodia,S. P. Singh 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.5

Twenty five haloalkaliphilic bacterial strains were isolated from sea water along the Coastal Gujarat (India) and screened for their ability to secret alkaline proteases. Among them, a potent strain S-20-9 (GenBank accession number EU118360), re-sembling to Halophilic Bacterium MBIC3303 on the basis of 16S rRNA gene sequencing, was selected for the optimization of enzyme production. S-20-9 produced protease optimally, under aerobic conditions during mid-stationary phase over a broad range of salt (5~25%, w/v) and pH (7~10). The optimum production was at pH 9 and 15% (w/v) NaCl. The production was suppressed by lactose, maltose, sucrose, and inorganic nitrogen sources, especially ammonium ions. Further, the pro-duction was significantly stimulated by KH₂PO₄ and suppressed by glucose. Similarly, the production was also suppressed at higher concentrations of gelatin, yeast extract, peptone, and casamino acids, indicating towards a threshold value for ni-trogen requirement. The growth and protease production were enhanced by mono-valent cation (KCl), while the divalent cations acted as inhibitors. The study holds significance as only few reports are available on the alkaline proteases from haloalkaliphilic bacteria, particularly those from moderate saline habitats.

치즈와 치아건강 역할

윤여창,R.S. Patel,E. Renner,S. Singh 한국낙농학회 1992 韓國酪農學會誌 Vol.14 No.4

충치는 식품의 당질과 치아 플라그의 세균 사이의 상호작용의 결과이다. 플라그의 세균은 설탕과 같은 발효 가능한 당질을 유기산으로 분해한다. 이러한 산생성은 플라그의 pH를 저하시켜 충치가 시작되는데, pH 5.7 이상에서 치아는 안전하고 그 이하에서는 충치가 발생할 수 있다. 치즈는 다른 식품과 비교해서 충치효과의 가능성이 가장 적었다. 치즈가 충치효과가 적을 뿐 아니라 충치를 예방할 수 있는 가능성이 충분히 있다. 치즈에 풍부하게 존재하는 칼슘과 인이 이 과정에 작용하여 치아 건강에 탁월한 식품으로서 기능을 수행한다.

Photoluminescence, thermoluminescence and evaluation of some parameters of Dy³⁺ activated Sr₅(PO₄)₃F phosphor synthesized by sol-gel method

Gupta, K.K.,Kadam, R.M.,Dhoble, N.S.,Lochab, S.P.,Singh, V.,Dhoble, S.J. Elsevier Sequoia 2016 JOURNAL OF ALLOYS AND COMPOUNDS Vol.688 No.2

In this study, we have synthesized Dy<SUP>3+</SUP> activated Sr<SUB>5</SUB>(PO<SUB>4</SUB>)<SUB>3</SUB>F (S-FAP) phosphors by sol-gel synthesis method. The synthesized phosphors were characterized by X-ray diffraction pattern (XRD), scanning electron microscopic (SEM), photoluminescence (PL) and thermoluminescence (TL) for structural, morphological and luminescent properties. Dy<SUP>3+</SUP> activated Sr<SUB>5</SUB>(PO<SUB>4</SUB>)<SUB>3</SUB>F phosphor shows its characteristic PL emission at 481 nm and 574 nm due to <SUP>4</SUP>F<SUB>9/2</SUB> → <SUP>6</SUP>H<SUB>15/2</SUB> and <SUP>4</SUP>F<SUB>9/2</SUB> → <SUP>6</SUP>H<SUB>13/2</SUB> transitions, respectively. TL characteristics of Dy<SUP>3+</SUP> doped Sr<SUB>5</SUB>(PO<SUB>4</SUB>)<SUB>3</SUB>F phosphors were taken after irradiation by <SUP>60</SUP>Co gamma exposure. Two separate TL peaks at 126 <SUP>o</SUP>C and 279 <SUP>o</SUP>C were observed in case of Dy<SUP>3+</SUP> doped phosphor. Sr<SUB>5</SUB>(PO<SUB>4</SUB>)<SUB>3</SUB>F:Dy<SUP>3+</SUP> phosphor was irradiated within a wide range of exposure of 50 Gy to 7 kGy doses. Linearity was found up to 2 kGy and thereafter TL response saturates. Fading study was also carried out over the duration of six weeks for Sr<SUB>5</SUB>(PO<SUB>4</SUB>)<SUB>3</SUB>F phosphor. Trapping parameters were calculated using Chen's peak shape method, initial rise method and various heating rate method. Glow curve is deconvoluted using computerized glow curve deconvolution program. TL sensitivity of Sr<SUB>5</SUB>(PO<SUB>4</SUB>)<SUB>3</SUB>F:Dy<SUP>3+</SUP> phosphor is found to be less by a factor of 2.08 than commercially used CaSO<SUB>4</SUB>:Dy phosphor. In order to identify the defect centers formed upon γ-ray irradiation in Sr<SUB>5</SUB>(PO<SUB>4</SUB>)<SUB>3</SUB>F:Dy<SUP>3+</SUP> phosphor, EPR measurements were carried out on un-irradiated and irradiated phosphors.

Combustion Synthesized Europium Doped LaAl₁₁O₁₈ Phosphors—An Electron Paramagnetic Resonance and Optical Study

Singh, Vijay,Sivaramaiah, G.,Rao, J. L.,Kumaran, R. Senthil,Dhoble, S. J. American Scientific Publishers 2016 Journal of Nanoscience and Nanotechnology Vol.16 No.1

<P>By adopting a facile solution combustion synthesis, crystallized europium doped blue phosphors were successfully prepared. These phosphors were characterized by X-ray diffraction (XRD), Fourier transform infrared absorption (FT-IR), energy dispersive analysis of X-ray, Electron Paramagnetic Resonance (EPR) and Photoluminescence (PL) experimental methods. The photoluminescence spectrum indicates Eu2+ and Eu3+ ions in these phosphors. The band at around 442 nm is attributed to the spin-allowed 4f(6)5d(1) (D-2) -> (4)f(7)(S-6(7/2)) transition of Eu2+ ions. The sharp bands at 591 (D-5(0) -> F-7(1)) and 616 (D-5(0) -> F-7(2)) nm are attributed to the spin-forbidden transitions of Eu3+ ions. The EPR spectra of as-prepared and post-treated LaAl11O18:Eu phosphors exhibit signals characteristic of La2+ and Eu2+ ions. The number of spins, Gibbs energy, magnetic susceptibility, Curie constant and effective magnetic moment values were calculated and compared at 296 and 110 K.</P>

Mass transfer kinetics using two-site interface model for removal of Cr(VI) from aqueous solution with cassava peel and rubber tree bark as adsorbents

M. Vasudevan,P. S. Ajithkumar,R. P. Singh,N. Natarajan 대한환경공학회 2016 Environmental Engineering Research Vol.21 No.2

Present study investigates the potential of cassava peel and rubber tree bark for the removal of Cr (VI) from aqueous solution. Removal efficiency of more than 99% was obtained during the kinetic adsorption experiments with dosage of 3.5 g/L for cassava peel and 8 g/L for rubber tree bark. By comparing popular isotherm models and kinetic models for evaluating the kinetics of mass transfer, it was observed that Redlich-Peterson model and Langmuir model fitted well (R2 > 0.99) resulting in maximum adsorption capacity as 79.37 mg/g and 43.86 mg/g for cassava peel and rubber tree bark respectively. Validation of pseudo-second order model and Elovich model indicated the possibility of chemisorption being the rate limiting step. The multi-linearity in the diffusion model was further addressed using multi-sites models (two-site series interface (TSSI) and two-site parallel interface (TSPI) models). Considering the influence of interface properties on the kinetic nature of sorption, TSSI model resulted in low mass transfer rate (5% for cassava peel and 10% for rubber tree bark) compared to TSPI model. The study highlights the employability of two-site sorption model for simultaneous representation of different stages of kinetic sorption for finding the rate-limiting process, compared to the separate equilibrium and kinetic modeling attempts.

Prediction of Hybrid Performance Based on the Genetic Distance of Parental Lines in Two-Line Rice (Oryza sativa L.) Hybrids

Vikas Kumar Singh,Priti Upadhyay,Pallavi Sinha,Ashish Kumar Mall,Ranjith K. Ellur,Atul Singh,Sanjay K.Jaiswal,Sunil Biradar,S. Ramakrishna,R.M. Sundaram,Ilyas Ahmed,B. C. Viraktamath,C. Kole,Sukhpal S 한국작물학회 2011 Journal of crop science and biotechnology Vol.14 No.1

A total of six TGMS (thermosensitive genic male sterile lines) and nine pollinator lines were subjected to molecular characterization using 48 genome-wide SSR (simple sequence repeat) markers. Cluster analysis revealed clear differentiation among the TGMS lines according to their source of origin. The SSR-based genetic distance between the hybrids of the parental lines ranged from 0.36to 0.79 suggesting a high degree of genetic divergence. Among a set of 54 hybrids generated using parental lines, 32 showed better parent heterosis (+21.7%) while 19 showed mid-parent heterosis (+15.0%). For the trait yield per plant genetic distance (GD) was negatively correlated with F1 performance (r = -0.202), mid-parent heterosis (r = -0.325*; P < 0.05), and better parent heterosis (r = -0.261), while it was positively correlated with specific combining ability (r = 0.042). Based on the grouped genetic distance (GGD),the hybrid combinations were divided into four groups. The GGD showed linear correlation with hybrid performance within the group (GGD = 40 - 50: r = -0.07; GGD = 70 - 80: r = 0.32). This information can be utilized in the development of higher yielding,two-line rice hybrids through selection of intermediately diverse parental lines using GGD.

Progressive failure of symmetric laminates under in-plane shear: Il-Negative shear

Singh, S.B.,Kumar, Ashwini,Iyengar, N.G.R. Techno-Press 1998 Structural Engineering and Mechanics, An Int'l Jou Vol.6 No.7

The objective of the present work is to estimate the strength and failure characteristics of symmetric thin square laminates under negative shear load. Two progressive failure analyses, one using the Hashin criterion and the other using a Tensor polynomial criterion, are used in conjunction with the finite element method. First-order shear-deformation theory along with geometric nonlinearity in the von Karman sense has been incorporated in the finite element modeling. Failure loads, associated maximum transverse displacements, locations and modes of failure including the onset of delamination are discussed in detail; these are found to be quite different from those for the positive sheer load reported in Part I of this study (Singh et al. 1998).

Progressive failure of symmetrically laminated plates under uni-axial compression

Singh, S.B.,Kumar, Ashwini,Iyengar, N.G.R. Techno-Press 1997 Structural Engineering and Mechanics, An Int'l Jou Vol.5 No.4

The objective of this work is to predict the failure loads, associated maximum transverse displacements, locations and the modes of failure, including the onset of delamination, of thin, flat, square symmetric laminates under the action of uni-axial compression. Two progressive failure analyses, one using Hashin criterion and the other using Tensor polynomial criteria, are used in conjunction with the finite element method. First order shear deformation theory and geometric nonlinearity in the von Karman sense have been employed. Five different types of lay-up sequence are considered for laminates with all edges simply supported. In addition, two boundary conditions, one with all edges fixed and other with mixed boundary conditions for $(+45/-45/0/90)_{2s}$ quasi-isotropic laminate have also been considered to study the effect of boundary restraints on the failure loads and the corresponding modes of failure. A comparison of linear and nonlinear results is also made for $({\pm}45/0/90)_{2s}$ quasi-isotropic laminate. It is observed that the maximum difference between the failure loads predicted by various criteria depend strongly on the laminate lay-ups and the flexural boundary restraints. Laminates with clamped edges are found to be more susceptible to failure due to the transverse shear and delamination, while those with the simply supported edges undergo total collapse at a load slightly higher than the fiber failure load.