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RISS 인기검색어
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- 저자 : Qiuping Huang (검색결과 4 건)
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Qiuping Huang,Chenyang Xie,Xinyu Li,Bin Jin,Qi Pang,Jia-Le Song 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
To explore the effect of LTA on clinical symptoms in letrozole-induced PCOS rats. Female SD rats were given letrozole for 28 consecutive days to induce the PCOS model. The PCOS rats were given LTA intervention(50 and 200 mg/kg) for 30 days. Changes in body weight and estrous cycle were observed. Serum levels of hormones and lipids were measured by ELISA assay. Protein levels of α-SMA, p-smad2, smad2, p-smad3, smad3 and smad4 were detected by Western Blotting assay. Compared with the normal group, the relative mass of abdominal fat, T, LH, INS, TG, TC and LDL-C were significantly increased, and the levels of E2, FSH and HDL-C were significantly decreased in PCOS rats. The protein levels of p-smad2, p-smad3, smad4 and α-SMA were significantly upregulated in the ovaries of PCOS rats(P<0.05). While the levels of T, LH, INS, TG, TC, LDL-C were decreased and E2, FSH and HDL-C level were increased by LTA treatment. Meanwhile, LTA reduced the protein levels of p-smad2, p-smad3, smad4, and α-SMA in the ovaries. The results suggested LTA was effective in regulating the hormone levels, improving metabolism, and alleviating ovarian fibrosis in PCOS rats.
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Yunwei Huang,Yanzhe Li,Zilun Tang,Qiuping Su,Tingting Liao,Yuxin Gu,Xiaofeng Lin,Xihong Zu,Wenjing Lin,Guobin Yi 한국고분자학회 2020 Macromolecular Research Vol.28 No.8
The four-arm star copolymers poly(methacrylic acid)-poly(2-hydroxyethyl methacrylate-disulfide~)-poly(poly(ethylene glycol) methyl ether methacrylate) (4AS-PMAAx-(PHEMA-SS~)y-PPEGMAz) with four different block ratios were synthesized and could self-assembled into cross-linked polymer micelles for the exploration of the structure-property relationship. The cross-linked polymer micelles in aqueous solution had low critical micelle concentration (CMC) values (1.9-4.6 mg/L), which exhibited better stability than non-cross-linked micelles. The CMC value decreased with the increase of the length of inner PMAA core and hydrophobic PHEMA cross-linked middle layer. The blank and doxorubicin (DOX)- loaded micelles with different block ratios were prepared by dialysis with the particle sizes of 120-240 nm. The longer inner PMAA core and cross-linked middle layer enhanced the drug loading content (DLC) results and led to relatively bigger particle sizes of polymer micelles. The in vitro DOX release data revealed that DOX-loaded micelles had low DOX cumulative release percentages of 18-37% after 110 h at pH 7.4, but up to 83-90% when introducing reductant GSH at pH 5.0. The 4AS-PMAA21.2-(PHEMASS~) 13.1-PPEGMA5.1 micelles with the longest PMAA core had the largest cumulative release of 90.1%. The DOX release process and mechanism of the micelles at different conditions fitted well with the semi-empirical equation. Overall, the results demonstrated that the block ratios and pH/redox-responsiveness of these four-arm star copolymers could be well-controlled and their self-assembled cross-linked micelles as anticancer drug carrier system could be improved by optimizing the different ratios.
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Protective activities of probiotics in letrozole-induced polycystic ovarian syndrome (PCOS) rats
Xinyu Li,Chenyang Xie,Qiuping Huang,Eun Mi Hwang,Bin Jin,Zhewen Dong,Zhen Zeng,Wen He,Qi Qi Pang,Jia-Le Song 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
To investigate the effect of Bifidobacterium, C. butyricum and L. plantarum on the letrozole induced PCOS rats. All female SD rats were randomly divided into five groups, modeling PCOS rats, and respectively given intragastric administration of Bifidobacterium, C. butyricum, and L. plantarum solution (1×10<SUP>9</SUP> CFU/mL) for 30 days, and observed body weight (BW). The serum levels of testosterone (T), LH, E2, FSH, INS, TG, TC, LDL, HDL were determined by ELISA kits. The levels of TGF-β1, smad4, smad3, p-smad3, p-smad2, smad2 were detected by Western Blotting. Compared with normal group, the BW, T, LH, INS, TG, TC LDL-C in PCOS rats were significantly increased, and the levels of E2, FSH and HDL-C were significantly decreased. Administrated with different probiotics can decrease the BW, T, LH, INS, TG, TC,LDL-C, and the levels of E2, FSH and HDL-C increased in PCOS rats. All probiotics significantly decreased the expression of p-smad2, p-smad3, smad4, TGF-β1. Our results showed that Bifidobacterium, C. butyricum and L. plantarum were all effective in regulating hormone levels, improving IR and dyslipidemia, and improving ovarian fibrosis in PCOS rats.
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Identification of DNA methylation and genetic alteration simultaneously from a single blood biopsy
Chen Xiaomin,Liu Jiahui,Li Jun,Xie Yinpeng,Yu Zichen,Shen Lu,Liu Qingfeng,Wu Wei,Zhao Qiang,Lin Haoxiang,Liu Gaotong,Luo Qiuping,Yang Ling,Huang Yi,Zhao Meiru,Yi Xin,Xia Xuefeng 한국유전학회 2023 Genes & Genomics Vol.45 No.5
Background High-throughput sequencing of blood cell-free DNA (cfDNA) techniques offer an opportunity to characterize and monitor cancer rapidly in a non-invasive and real-time manner. Nonetheless, there lacks a tool within therapeutic arsenal to identify multi-omics alterations simultaneously from a single biopsy. In current times, bisulfite-based sequencing detects 5mC and 5hmC at single-base resolution is the golden standard of DNA methylation, while the degradation of DNA and biased sequencing data are the problems of this method. Objective To identify the consistency analysis of methylation and genetic variation with single library, we presented a platform detecting multi-omics data simultaneously from a single blood biopsy using bisulfite-free method of genomic methylation sequencing (GM-seq) mediated by TET enzyme. Methods We detected methylomic and genetic changes simultaneously from a single blood biopsy in NA12878 and randomly chose ten blood biopsies from colorectal cancer or lung cancer patients to validate the ability of GM-seq. Results Similar cytosine methylation level between whole genome bisulfite sequencing (WGBS) and GM-seq were identified in NA12878. Moreover, longer insert size, CpGs coverage and GC distribution were outperformed than WGBS. In addition, the comparison of the single nucleotide polymorphism (SNP), insertion-deletion (Indel) and copy number variation (CNV) in NA12878 or ctDNA from liver cancer between GM-seq and whole genome sequencing (WGS) show a good consistency, indicating that this method is feasible for detecting genetic variation in blood. Conclusion In conclusion, our work demonstrated a method for identification of the methylated modification and genetic variations simultaneously from a single blood biopsy.
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