Multivariate Probabilistic Seismic Demand Model for the Bridge Multidimensional Fragility Analysis

Qi’ang Wang,Ziyan Wu,Shukui Liu 대한토목학회 2018 KSCE Journal of Civil Engineering Vol.22 No.9

Seismic fragility analysis for bridges is an essential issue for risk assessment of transportation networks exposed to seismic hazards. Considering multiple Performance Limit States (PLSs) and seismic demand parameters, the study proposes a multidimensional fragility evaluation methodology for engineering structures, and the objective of the paper is to show that the uncertainty and dependence between seismic demand parameters should be considered for fragility analysis. Thus, a new Probabilistic Seismic Demand Model (PSDM) following multivariate logarithmic normal distribution is addressed. Taking PLS correlation into consideration, multidimensional PLS formula is constructed to identify the structural failure domain. A RC bridge is studied to show the proposed theory. To consider bridge column plastic deformation and bearing nonlinear characteristic, nonlinear dynamic analyses are carried out. The bridge multidimensional fragility curves are derived and compared with fragility curves for an individual component. Results indicate that uncertainty and dependence of demand parameters can be properly dealt with by the multivariate PSDM. The multidimensional fragility is higher than fragility of any individual component, and the bridge as a system is more fragile. The ignorance of multiple components contribution to the system will generate an overestimation for the whole structural performance, which is adverse to engineering structural safety.

certainty and Dependence Analysis of Performance Limit State for Structural Multidimensional Fragility Evaluation

Qi’ang Wang,Ziyan Wu,Lu Liu 대한토목학회 2017 KSCE Journal of Civil Engineering Vol.21 No.4

Considering uncertainty and dependence of performance limit states (PLSs), the study addresses a methodology to evaluate multidimensional fragility. The purpose is to identify the PLS uncertainty quantitatively. The dependence between each PLS parameters is also investigated. The limit state band is firstly proposed to describe the bi-dimensional case. Through interval estimation, the band area with a certain confidence level is determined. A reinforcement concrete bridge is used as example to illustrate the proposed approach for developing fragility curves. PLS threshold samples are obtained to formulate limit state function using incremental dynamic analysis. The study investigates the sensitivity of the method for fragility assessment when different confidence levels are considered. In addition, influence of correlation coefficient between PLSs is evaluated. Results show that a fragility interval is obtained with the introduction of limit state band. The interval length decreases as with the reduction of the confidence level. The probability of failure becomes smaller when the dependence between PLSs is ignored, which will result in overestimation of the structural seismic performance

An Adaptively Speculative Execution Strategy Based on Real-Time Resource Awareness in a Multi-Job Heterogeneous Environment

( Qi Liu ),( Weidong Cai ),( Qiang Liu ),( Jian Shen ),( Zhangjie Fu ),( Xiaodong Liu ),( Nigel Linge ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.2

MapReduce (MRV1), a popular programming model, proposed by Google, has been well used to process large datasets in Hadoop, an open source cloud platform. Its new version MapReduce 2.0 (MRV2) developed along with the emerging of Yarn has achieved obvious improvement over MRV1. However, MRV2 suffers from long finishing time on certain types of jobs. Speculative Execution (SE) has been presented as an approach to the problem above by backing up those delayed jobs from low-performance machines to higher ones. In this paper, an adaptive SE strategy (ASE) is presented in Hadoop-2.6.0. Experiment results have depicted that the ASE duplicates tasks according to real-time resources usage among work nodes in a cloud. In addition, the performance of MRV2 is largely improved using the ASE strategy on job execution time and resource consumption, whether in a multi-job environment.

Prevalence and Related Factors of EPS among Schizophrenic Inpatients

Liu, Lian-Qi,Liu, Xian-Chen,Hu, Lei,Chen, Kun,Li, Chuan-Qi 용인정신의학연구소 1998 용인정신의학보 Vol.5 No.1

An Optimization Scheme in MapReduce for Reduce Stage

Qi Liu,Weidong Cai,Baowei Wang,Zhangjie Fu,Nigel Linge 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.8

As a widely used programming model for the purposes of processing large data sets, MapReduce (MR) becomes inevitable in data clusters or grids, e.g. a Hadoop environment. Load balancing as a key factor affecting the performance of map resource distribution, has recently gained high concerns to optimize. Current MR processes in the realization of distributed tasks to clusters use hashing with random modulo operations, which can lead to uneven data distribution and inclined loads, thereby obstruct the performance of the entire distribution system. In this paper, a virtual partition consistent hashing (VPCH) algorithm is proposed for the reduce stage of MR processes, in order to achieve such a trade-off on job allocation. Besides, experienced programmers are needed to decide the number of reducers used during the reduce phase of the MR, which makes the quality of MR scripts differ. So, an extreme learning method is employed to recommend potential number of reducer a mapped task needs. Execution time is also predicted for user to better arrange their tasks. According to the results, VPCH can lead to load balancing and our prediction model can provide fast prediction than SVM with similar accuracy maintained.

Blooms of the woloszynskioid dinoflagellate Tovellia diexiensis sp. nov. (Dinophyceae) in Baishihai Lake at the eastern edge of Tibetan Plateau

Qi Zhang,Huan Zhu,Zhengyu Hu,Guoxiang Liu 한국조류학회I 2016 ALGAE Vol.31 No.3

Freshwater red tides due to dinoflagellates have caused spectacular and regular “summer reddening” in recent years in Baishihai Lake, a temperate, meromictic, meso- or oligotrophic, high-altitude, landslide-dammed, deep lake located at the eastern edge of Tibetan Plateau in China. Based on morphological and molecular analyses, the causative organism has been identified as a new woloszynskioid dinoflagellate, Tovellia diexiensis Q. Zhang et G. X. Liu sp. nov. The vegetative cells are 20–32 μm long and 16–24 μm wide. They have a hemispherical episome and a broadly rounded hyposome with a short characteristic antapical spine. Usually cells are bright red due to the presence of numerous red-pigmented bodies, which often masked the yellowish green discoid chloroplasts. The amphiesma of motile cells comprise mainly quadrilateral, pentagonal or hexagonal thin plates, arranged in 4–5 latitudinal series on the episome, 1 in the cingulum and 4 on the hyposome. Molecular phylogenies based on small subunit ribosomal DNA and large subunit ribosomal DNA (LSU) indicate T. diexiensis from Baishihai Lake to belong to the family Tovelliaceae, which was monophyletic in our LSU phylogenies. During the bloom-forming period in 2005, cell density of T. diexiensis reached 9.15 × 105 cells L−1. Astaxanthin and its diester were found to be the major pigments in T. diexiensis, resulting in a characteristic blood-red color of the water in Baishihai Lake.

The CREG1-FBXO27-LAMP2 axis alleviates diabetic cardiomyopathy by promoting autophagy in cardiomyocytes

Liu Dan,Xing Ruinan,Zhang Quanyu,Tian Xiaoxiang,Qi Yanping,Song Haixu,Liu Yanxia,Yu Haibo,Zhang Xiaolin,Jing Quanmin,Yan Chenghui,Han Yaling 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Autophagy plays an important role in the development of diabetic cardiomyopathy. Cellular repressor of E1A-stimulated genes 1 (CREG1) is an important myocardial protective factor. The aim of this study was to investigate the effects and mechanisms of CREG1 in diabetic cardiomyopathy. Male C57BL/6 J mice, Creg1 transgenic mice and cardiac-specific knockout mice were used to establish a type 2 diabetes model. Small animal ultrasound, Masson’s staining and western blotting were used to evaluate cardiac function, myocardial fibrosis and autophagy. Neonatal mouse cardiomyocytes (NMCMs) were stimulated with palmitate, and the effects of CREG1 on NMCMs autophagy were examined. CREG1 deficiency exacerbated cardiac dysfunction, cardiac hypertrophy and fibrosis in mice with diabetic cardiomyopathy, which was accompanied by exacerbated autophagy dysfunction. CREG1 overexpression improved cardiac function and ameliorated cardiac hypertrophy and fibrosis in diabetic cardiomyopathy by improving autophagy. CREG1 protein expression was decreased in palmitate-induced NMCMs. CREG1 knockdown exacerbated cardiomyocyte hypertrophy and inhibited autophagy. CREG1 overexpression inhibited cardiomyocyte hypertrophy and improved autophagy. LAMP2 overexpression reversed the effect of CREG1 knockdown on palmitate-induced inhibition of cardiomyocyte autophagy. CREG1 inhibited LAMP2 protein degradation by inhibiting the protein expression of F-box protein 27 (FBXO27). Our findings indicate new roles of CREG1 in the development of diabetic cardiomyopathy.

Molecular Cloning, Expression and Characterizaion of Heat Shock Protein Gene from Paecilomyces tenuipes Jocheon-1

Liu Ya Qi,Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05

In this study, a full-length heat shock protein88 complementary DNA (cDNA) of Paecilomyces tenuipes Jocheon-1 was obtained by screening of P. tenuipesJocheon-1 Uni-Zap cDNA library and 5' RACE polymerase chain reaction. The Paecilomyces tenuipes Jocheon-1 heat shock protein88 cDNA contains an open reading frame of 2,139 bp encoding 713 amino acid residues. The deduced amino acid sequence of the P. tenuipes Jocheon-1 HSP88 cDNA showed 77% identity to N. haematococca HSP88 and 45-76% identity to other fungi HSP88. Phylogenetic analysis and BLAST program analysis confirmed that the deduced amino acid sequences of the P. tenuipes Jocheon-1 HSP88 gene belonged to the ascomycetes group within the fungal clade and P. tenuipes Jocheon-1 HSP88 also contains the conserved ATPase domain at the N-terminal. The cDNA encoding P. tenuipes Jocheon-1 HSP88 was expressed as a 88 kDa polypeptide in baculovirus-infected insect Sf9 cells. Under different stress conditions, mRNA expression of P. tenuipes Jocheon-1 HSP88 were quantified by real-time PCR and the result showed that heat shock stress affected the mRNA expression levels of P. tenuipes Jocheon-1 HSP88.

Applications of Bacterial Cellulose-Based Composite Materials in Hard Tissue Regenerative Medicine

Liu Yingyu,Liu Haiyan,Guo Susu,Qi Jin,Zhang Ran,Liu Xiaoming,Sun Lingxiang,Zong Mingrui,Cheng Huaiyi,Wu Xiuping,Shanxi Medical University School and Hospital of Stomatology 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.7

BACKGROUND: Cartilage, bone, and teeth, as the three primary hard tissues in the human body, have a significant application value in maintaining physical and mental health. Since the development of bacterial cellulose-based composite materials with excellent biomechanical strength and good biocompatibility, bacterial cellulose-based composites have been widely studied in hard tissue regenerative medicine. This paper provides an overview of the advantages of bacterial cellulose-based for hard tissue regeneration and reviews the recent progress in the preparation and research of bacterial cellulose-based composites in maxillofacial cartilage, dentistry, and bone. METHOD: A systematic review was performed by searching the PubMed and Web of Science databases using selected keywords and Medical Subject Headings search terms. RESULTS: Ideal hard tissue regenerative medicine materials should be biocompatible, biodegradable, non-toxic, easy to use, and not burdensome to the human body; In addition, they should have good plasticity and processability and can be prepared into materials of different shapes; In addition, it should have good biological activity, promoting cell proliferation and regeneration. Bacterial cellulose materials have corresponding advantages and disadvantages due to their inherent properties. However, after being combined with other materials (natural/ synthetic materials) to form composite materials, they basically meet the requirements of hard tissue regenerative medicine materials. We believe that it is worth being widely promoted in clinical applications in the future. CONCLUSION: Bacterial cellulose-based composites hold great promise for clinical applications in hard tissue engineering. However, there are still several challenges that need to be addressed. Further research is needed to incorporate multiple disciplines and advance biological tissue engineering techniques. By enhancing the adhesion of materials to osteoblasts, providing cell stress stimulation through materials, and introducing controlled release systems into matrix materials, the practical application of bacterial cellulose-based composites in clinical settings will become more feasible in the near future.

Dendritic Cells Induce Specific Cytotoxic T Lymphocytes against Prostate Cancer TRAMP-C2 Cells Loaded with Freeze-thaw Antigen and PEP-3 Peptide

Liu, Xiao-Qi,Jiang, Rong,Li, Si-Qi,Wang, Jing,Yi, Fa-Ping Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.2

Prostate cancer is the most common cancer in men. In this study, we investigated immune responses of cytotoxic T lymphocytes (CTLs) against TRAMP-C2 prostate cancer cells after activation by dendritic cells (DCs) loaded with TRAMP-C2 freeze-thaw antigen and/or PEP-3 peptide in vitro. Bone marrow-derived DC from the bone marrow of the C57BL/6 were induced to mature by using the cytokine of rhGM-CSF and rhIL-4, and loaded with either the freeze-thaw antigen or PEP-3 peptide or both of them. Maturation of DCs was detected by flow cytometry. The killing efficiency of the CTLs on TRAMP-C2 cells were detected by flow cytometry, CCK8, colony formation, transwell migration, and wound-healing assay. The levels of the IFN-${\gamma}$, TNF-${\beta}$ and IL-12 were measured by enzyme-linked immunosorbent assay (ELISA). Compared with the unloaded DCs, the loaded DCs had significantly increased expression of several phenotypes related to DC maturation. CTLs activated by DCs loaded with freeze-thaw antigen and PEP-3 peptide had more evident cytotoxicity against TRAMP-C2 cells in vitro. The secretion levels of IFN-${\gamma}$, TNF-${\beta}$ and IL-12, secreted by DCs loaded with antigen and PEP-3 and interaction with T cells, were higher than in the other groups. Our results suggest that the CTLs activated by DCs loaded with TRAMP-C2 freeze-thaw antigen and PEP-3 peptide exert a remarkable killing efficiency against TRAMP-C2 cells in vitro.