Poster Presentations : Basic Research, Parenteral Nutrition ; Serum Vitamin a Levels of Healthy People in Northeastern Thailand

( Patcharee Boonsiri ),( Jureerut Pooart ),( Roongpet Tangrasameeprasert ),( Pranithi Hongprabhas ),( Siriporn Patrakitkomjorn ),( Puangrat Yongvanit ) 한국정맥경장영양학회 2005 The 11th PENSA Congress Vol.2005 No.-

Oral Presentations : Nutritional Assessment ; Tomatoes Intake Inversely Related to Cholesterol Levels in Northeastern Thai Elderly

( Prasit Suwannalert ),( Patcharee Boonsiri ),( Tueanjit Khampitak ),( Kovit Khampitak ),( Maitree Suttajit ),( Pote Sriboonlue ),( Puangrat Yongvanit ) 한국정맥경장영양학회 2005 The 11th PENSA Congress Vol.2005 No.-

Oral Presentations : Nutritional Assessment ; Serum Zinc Levels of Healthy People in Northeastern Thailand

( Jureerut Pooart ),( Patcharee Boonsiri ),( Roongpet Tangrasameeprasert ),( Pranithi Hongprabhas ),( Limthong Weerachatyanukul ),( Puangrat Yongvanit ) 한국정맥경장영양학회 2005 The 11th PENSA Congress Vol.2005 No.-

Cytotoxic Effects of Phytophenolics from Caesalpinia mimosoides Lamk on Cervical Carcinoma Cell Lines through an Apoptotic Pathway

Palasap, Adisak,Limpaiboon, Temduang,Boonsiri, Patcharee,Thapphasaraphong, Suthasinee,Daduang, Sakda,Suwannalert, Prasit,Daduang, Jureerut Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1

Background: Extracts of Caesalpinia mimosoides Lamk has been reported to possess anticancer effects, but the active ingredients and the anti-cancer mechanisms are still unknown. Materials and Methods: The effects of a C mimosoides Lamk extract on cell proliferation and apoptosis induction in human cervical carcinoma cell lines, namely HeLa, SiHa, and C33A, as well as in normal Vero cells, were investigated. Results: Treatment with 5 active fractions (F17-F21) of C mimosoides Lamk methanol extracts inhibited cell viability in a dose- and time-dependent manner. Neutral red assays indicated that treatment with F21 significantly decreased the viability of all cervical cancer cell lines compared to F21-treated normal cells. In addition, HPLC analysis revealed that F21 contained multiple phenolic compounds, namely gallic acid, caffeine, vanillic acid, ferulic acid and resveratrol. F21 had the lowest IC50 and, therefore, a much higher cytotoxicity than F20, F17, F19, and F18 by 20-, 25-, 46- and 47- fold, respectively. Analysis of activation of the apoptosis pathway using a caspase 3/7 activity assay revealed that F21 treatment resulted in a considerable increase in caspase activation in all cancer cell lines tested. At the same concentration of F21, HeLa cells had the highest caspase activity (6.5-fold) compared to the control. Conclusion: C mimosoides Lamk may be of value as an alternative therapeutic agent, especially in combination with other compounds offering possible of synergy of action. Moreover, HPV- and non-HPV-related cervical cancer cells may differ in their responses to treatment regimens.

Gallic Acid Enhancement of Gold Nanoparticle Anticancer Activity in Cervical Cancer Cells

Daduang, Jureerut,Palasap, Adisak,Daduang, Sakda,Boonsiri, Patcharee,Suwannalert, Prasit,Limpaiboon, Temduang Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.1

Cervical cancer (CxCa) is the most common cancer in women and a prominent cause of cancer mortality worldwide. The primary cause of CxCa is human papillomavirus (HPV). Radiation therapy and chemotherapy have been used as standard treatments, but they have undesirable side effects for patients. It was reported that gallic acid has antioxidant, antimicrobial, and anticancer activities. Gold nanoparticles are currently being used in medicine as biosensors and drug delivery agents. This study aimed to develop a drug delivery agent using gold nanoparticles conjugated with gallic acid. The study was performed in uninfected (C33A) cervical cancer cells, cervical cancer cells infected with HPV type 16 (CaSki) or 18 (HeLa), and normal Vero kidney cells. The results showed that GA inhibited the proliferation of cancer cells by inducing apoptosis. To enhance the efficacy of this anticancer activity, 15-nm spherical gold nanoparticles (GNPs) were used to deliver GA to cancer cells. The GNPs-GA complex had a reduced ability compared to unmodified GA to inhibit the growth of CxCa cells. It was interesting that high-concentration ($150{\mu}M$) GNPs-GA was not toxic to normal cells, whereas GA alone was cytotoxic. In conclusion, GNPs-GA could inhibit CxCa cell proliferation less efficiently than GA, but it was not cytotoxic to normal cells. Thus, gold nanoparticles have the potential to be used as phytochemical delivery agents for alternative cancer treatment to reduce the side effects of radiotherapy and chemotherapy.

Inhibitory Effects of Gallic Acid Isolated from Caesalpinia mimosoides Lamk on Cholangiocarcinoma Cell Lines and Foodborne Pathogenic Bacteria

Rattanata, Narintorn,Klaynongsruang, Sompong,Daduang, Sakda,Tavichakorntrakool, Ratree,Limpaiboon, Temduang,Lekphrom, Ratsami,Boonsiri, Patcharee,Daduang, Jureerut Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.3

Gallic acid was isolated from Caesalpinia mimosoides Lamk and the structure s identified based on spectroscopic analysis and comparison with authentic compound. In this study we compared the ability of natural gallic acid (nGA) and commercial gallic acid (cGA) to inhibit the proliferation of cholangiocarcinoma cell lines (M213, M214) and foodborne pathogenic bacteria (Salmonella spp. and Plesiomonas shigelloides). Both nGA and cGA had the same inhibitory effects on cell proliferation by inducing apoptosis of cholangiocarcinoma cell lines. In addition, nGA inhibited growth of foodborne pathogenic bacteria in the same manner as cGA. Our results suggest that nGA from Caesalpinia mimosoides Lamk is a potential anticancer and antibacterial compound. However, in vivo studies are needed to elucidate the specific mechanisms involved.

Effects of Qigong exercise on muscle strengths and oxidative stress/antioxidant responses in young sedentary females: a quasi-experimental study

Kultida Klarod,Sanita Singsanan,Niramon Thamwiriyasati,Suphannika Ladawan,Nongnuch Luangpon,Patcharee Boonsiri,Martin Burtscher 한국운동재활학회 2020 JER Vol.16 No.5

Risk factors for canine magnesium ammonium phosphate urolithiasis associated with bacterial infection

Nahathai Uttamamul,Supranee Jitpean,Aroonlug Lulitanond,Lumyai Wonglakorn,Nattaya Sae-ung,Patcharee Boonsiri,Jureerut Daduang,Ratree Tavichakorntrakool 대한수의학회 2022 Journal of Veterinary Science Vol.23 No.1

Background: With limited information available, the association among urinary tract infections, urease-producing bacteria and the presence of magnesium ammonium phosphate (MAP) urolithiasis in canines in Thailand requires more study. Objectives: This study aimed to investigate the association between demographic characteristics of canines and the presence of MAP urolithiasis in canines, and to evaluate antimicrobial susceptibility patterns of bacteria isolated from canine uroliths. Methods: A total of 56 canines admitted for treatment with surgical removal of uroliths were recruited. Demographic characteristics and clinical chemistry data were recorded. Bacteria isolated from the removed uroliths were identified. Chemical compositions of the uroliths were analyzed by Fourier transform infrared spectrometer. Potential risk factors were determined with univariable and multivariable logistic regression analyses. Results: Of 56 canine urolithiasis, bacteria were isolated from uroliths of 38 canines (27 MAP and 11 non-MAP) but not from uroliths of 18 canines (5 MAP and 13 non-MAP). The most common bacteria found in nidus of MAP uroliths was Staphylococcus pseudintermedius (approximately 51%). An antimicrobial resistance was frequently found in Staphylococci isolates (42.86%). Multivariate logistic regression analysis showed that the predictors of MAP urolith in canine urolithiasis were being female (p = 0.044; adjusted odds ratio [OR], 10.22; 95% confidence interval [CI], 1.06–98.24) and the positive urolith culture (p = 0.012; adjusted OR, 8.60; 95% CI, 1.60–46.30). Conclusions: Our results indicate that S. pseudintermedius (a urease-producing bacterium) is the major causative bacteria of MAP uroliths. A positive urolith culture and being female are risk factors of MAP urolithiasis in canines.

In vitro and In vivo Antitumor Activity of Tiliacorinine in Human Cholangiocarcinoma

Janeklang, Somkid,Nakaew, Archawin,Vaeteewoottacharn, Kulthida,Seubwai, Wunchana,Boonsiri, Patcharee,Kismali, Gorkem,Suksamrarn, Apichart,Okada, Seiji,Wongkham, Sopit Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17

Cholangiocarcinoma (CCA) is a fatal cancer with poor prognosis and less than 10% of CCA patients can be offered surgical cure. Conventional chemotherapy results in unfavorable outcomes. At present, plant-derived compounds are gaining interest as potential cancer therapeutics, particularly for treatment-refractory cancers. In this study, antitumor activity of tiliacorinine, the major alkaloid isolated from a tropical plant, on CCA was first demonstrated. Antiproliferative effects of tiliacorinine on human CCA cell lines were investigated using SRB assays. Acridine orange/ethidium bromide staining, flow cytometric analysis and DNA laddering assays were used for apoptotic determination. Apoptosis-related proteins were verified by Western blotting and antitumor activity of tiliacorinine in vivo was demonstrated in CCA xenografted mice. Tiliacorinine significantly inhibited proliferation of human CCA cell lines with $IC_{50}$ $4.5-7{\mu}M$ by inducing apoptosis through caspase activation, upregulation of BAX, and downregulation of $Bcl_{xL}$ and XIAP. Tiliacorinine considerably reduced tumor growth in CCA xenografted mice. These results demonstrated antitumor effects of tiliacorinine on human CCA in vitro and in vivo. Tiliacorinine may be an effective agent for CCA treatment.

Attenuated total reflection Fourier transform infrared (ATR-FTIR) as a primary screening method for cancer in canine serum

Arayaporn Macotpet,Ekkachai Pattarapanwichien,Sirinart Chio-Srichan,Jureerut Daduang,Patcharee Boonsiri 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.1

Cancer is a major cause of death in dogs worldwide, and the incidence of cancer in dogs is increasing. The attenuated total reflection Fourier transform infrared spectroscopic (ATR-FTIR) technique is a powerful tool for the diagnosis of several diseases. This method enables samples to be examined directly without pre-preparation. In this study, we evaluated the diagnostic value of ATR-FTIR for the detection of cancer in dogs. Cancer-bearing dogs (n = 30) diagnosed by pathologists and clinically healthy dogs (n = 40) were enrolled in this study. Peripheral blood was collected for clinicopathological diagnosis. ATR-FTIR spectra were acquired, and principal component analysis (PCA) was performed on the full wave number spectra (4,000-–650 cm-1). The leave-one-out cross validation technique and partial least squares (PLS) regression analysis were used to predict normal and cancer spectra. Red blood cell counts, hemoglobin levels and white blood cell counts were significantly lower in cancer-bearing dogs than in clinically healthy dogs (Pp < 0.01, Pp < 0.01 and Pp = 0.03, respectively). ATR-FTIR spectra showed significant differences between the clinically healthy and cancer-bearing groups. This finding demonstrates that ATR-FTIR can be applied as a screening technique to distinguish between cancer-bearing dogs and healthy dogs.