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[PF-0006] Morphological characterization of mustard (Brassica juncea) germplasm from Korea
Seong-Hoon Kim(Seong-Hoon Kim),Bum-Soo Hahn(Bum-Soo Hahn),Ho Chul Ko(Ho Chul Ko),Onsook Hur(Onsook Hur),Aejin Hwang(Aejin Hwang),Bichsaem Kim(Bichsaem Kim),Yoon-Jung Lee(Yoon-Jung Lee),Parthiban Subra 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-
Evaluation of Bioassay Methods to Assess Bacterial Soft Rot Resistance in Radish Cultivars
Tania Afroz(타니아 아프로즈),Onsook Hur(허온숙),Nayoung Ro(노나영),Jae-eun Lee(이재은),Aejin Hwang(황애진),Bichsaem Kim(김빛샘),Awraris Derbie Assefa(아와리스 데비 아세파),Ju Hee Rhee(이주희),Jung Sook Sung(성정숙),Ho-sun Lee(이호선),Bum-So 한국생명과학회 2021 생명과학회지 Vol.31 No.7
세균성 무름병 균[Pectobacterium carotovorum subsp. carotovorum (Pcc)]에 의해서 일어나는 무름병은 아시아 국가에서 재배되는 무에 있어서 심각한 질병 중의 하나로 알려져 있다. 이번 연구의 목적은 상업적으로 시판되는 무 품종의 세균성 무름병의 저항성에 대한 효율적인 생물검정법을 확립하고자 하였다. 첫째, 무 품종에 대해 세균성 무름병의 효율적인 생물검정 방법을 조사하였다. 6개의 무 품종을 다양한 조건[두 가지 온도(25℃와 30℃), 3가지 접종방법(관주, 분무, 침지), 두 발생단계(2와 4잎 단계)]으로 조사하였다. 연구 결과는 무름병균 1×106 cfu/ml 농도를 분무한 4잎 단계와 30℃에서 배양한 생물검정 방법이 무 품종에 대해 가장 효율적인 방법임을 알 수 있었다. 둘째, 41개의 무 품종에 5가지 세균(KACC 10225, KACC 10343, KACC 10421, KACC 10458, KACC 13953)을 접종하여 저항성을 조사하였다. KACC 10421가 세균성 무름병의 감수성 및 저항성 질병 정도를 가장 잘 나타냈다. 41개의 무 품종 중 13개는 무름병 균에 대해 중도 저항성을 나타냈고 28개는 감수성을 나타냈다. 이 연구에서 중도저항성 무 품종은 세균성 무름병 저항성 육종을 위한 저항성 자원으로 활용 가능하고 육종가, 농민, 연구자, 최종 소비자에 의해서 다양한 목적을 위해 사용 가능할 것으로 사료된다. Bacterial soft rot, caused by Pectobacterium carotovorum subsp. carotovorum (Pcc), is one of the destructive diseases of radish (Raphanus sativus) in Asian countries. The objective of this study was to establish an efficient bioassay method for the evaluation of bacterial soft rot resistance in commercial radish cultivars. First, an efficient bioassay method for examining resistance to bacterial soft rot in commercial radish cultivars was investigated. Six commercial radish cultivars were tested under various conditions: two temperatures (25℃ and 30℃), three inoculations methods (drenching, spraying, and root dipping), and two growth stages (two- and four-leaf stages). The results suggested that spraying with 1×106 cfu/ml of bacterial inoculums during the four-leaf stage and incubating at 30℃ could be the most efficient screening method for bacterial soft rot resistance in commercial radish cultivars. Second, we investigated the degree of resistance of 41 commercial radish cultivars to five Pcc isolates, namely KACC 10225, KACC 10343, KACC 10421, KACC 10458, and KACC 13953. KACC 10421 had the strongest susceptibility in terms of moderately resistant disease response to bacterial soft rot. Out of the 41 radish cultivars, 13 were moderately resistant to this pathogen, whereas 28 were susceptible. The moderately resistant radish cultivars in this investigation could serve as resistance donors in the breeding of soft rot resistance or could be used to determine varietal improvement for direct use by breeders, scientists, farmers, researchers, and end customers.
Evaluation of Pepper Genetic Resources for Contents of Vitamin C and Capsaicinoids
Sukyeung Lee,Nayoung No,Onsook Hur,Yumi Choi,Bora Geum,Bum-Soo Hahn 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
Pepper is known for its high content of vitamin C (ascorbic acid) and capsaicin. The well-known ‘capsaicin’ is one of the derivatives of capsaicinoids. In this study, ascorbic acid and capsaicinoids were evaluated for 259 pepper germplasms in National Agrobiodiversity Center in Korea. Ascorbic acid and capsaicinoids were extracted with solvent meta-phosphoric acid : ethanol=8:2 and MeOH, respectively, and analyzed by HPLC. Capsaicinoids content was distributed from 0 to 1230.58mg/100g,DW. There were 73 accessions with no spiciness. There were 25 accessions with a content of 1,000mg/100g, DW or more. Among them, IT221893 (“KC01297” originated from Laos) was the highest. Ascorbic acid content was distributed 0 to 2001.5 mg/100g,DW. The resources with a content of 1600 or more were 12 accessions including the highest one, IT158552 (“Annabelle” originated from Austria). Eleven of these resources with high vitamin C content were found to have zero capsaicinoids. This shows that non-spicy peppers tend to have high vitamin C content. These results will serve as a basis data for Korean pepper breeding program.
김빛샘(Bichsaem Kim),허온숙(Onsook Hur),이재은(Jae-Eun Lee),Awraris Derbie Assefa,고호철(Ho-Cheol Ko),정윤조(Yun-Jo Chung),이주희(Ju-hee Rhee),한범수(Bum-Soo Hahn) 한국자원식물학회 2021 한국자원식물학회지 Vol.34 No.6
The edible roots of radish (Raphanus sativus L.) are consumed worldwide. For characterization and evaluation of the agronomic traits and health-promoting chemicals in radish germplasms, new germplasm breeding materials need to be identified. The objectives of this study were to evaluate the phenotypic traits and glucosinolate contents of radish roots from 110 germplasms, by analyzing correlations between 10 quantitative phenotypic traits and the individual and total contents of five glucosinolates. Phenotypic characterization was performed based on descriptors from the UPOV and IBPGR, and glucosinolate contents were analyzed using liquid chromatography-tandem mass spectrometry in multiple reaction monitoring mode (MRM). Regarding the phenotypic traits, a significant correlation between leaf length and root weight was observed. Glucoraphasatin was the main glucosinolate, accounting for an average of 71% of the total glucosinolates in the germplasms; moreover, its content was significantly correlated with that of glucoerucin, its precursor. Principal component analysis indicated that the 110 germplasms could be divided into five groups based on their glucosinolate contents. High levels of free-radical scavenging activity (DPPH) were observed in red radishes. These results shed light on the beneficial traits that could be targeted by breeders, and could also promote diet diversification by demonstrating the health benefits of various germplasms.