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Aquatide Activation of SIRT1 Reduces Cellular Senescence through a SIRT1-FOXO1-Autophagy Axis
Lim, Chae Jin,Lee, Yong-Moon,Kang, Seung Goo,Lim, Hyung W.,Shin, Kyong-Oh,Jeong, Se Kyoo,Huh, Yang Hoon,Choi, Suin,Kor, Myungho,Seo, Ho Seong,Park, Byeong Deog,Park, Keedon,Ahn, Jeong Keun,Uchida, Yos The Korean Society of Applied Pharmacology 2017 Biomolecules & Therapeutics(구 응용약물학회지) Vol.25 No.5
Ultraviolet (UV) irradiation is a relevant environment factor to induce cellular senescence and photoaging. Both autophagy- and silent information regulator T1 (SIRT1)-dependent pathways are critical cellular processes of not only maintaining normal cellular functions, but also protecting cellular senescence in skin exposed to UV irradiation. In the present studies, we investigated whether modulation of autophagy induction using a novel synthetic SIRT1 activator, heptasodium hexacarboxymethyl dipeptide-12 (named as Aquatide), suppresses the UVB irradiation-induced skin aging. Treatment with Aquatide directly activates SIRT1 and stimulates autophagy induction in cultured human dermal fibroblasts. Next, we found that Aquatide-mediated activation of SIRT1 increases autophagy induction via deacetylation of forkhead box class O (FOXO) 1. Finally, UVB irradiation-induced cellular senescence measured by $SA-{\beta}-gal$ staining was significantly decreased in cells treated with Aquatide in parallel to occurring SIRT1 activation-dependent autophagy. Together, Aquatide modulates autophagy through SIRT1 activation, contributing to suppression of skin aging caused by UV irradiation.
Aquatide Activation of SIRT1 Reduces Cellular Senescence through a SIRT1-FOXO1-Autophagy Axis
( Chae Jin Lim ),( Yong-moon Lee ),( Seung Goo Kang ),( Hyung W. Lim ),( Kyong-oh Shin ),( Se Kyoo Jeong ),( Yang Hoon Huh ),( Suin Choi ),( Myungho Kor ),( Ho Seong Seo ),( Byeong Deog Park ),( Keedo 한국응용약물학회 2017 Biomolecules & Therapeutics(구 응용약물학회지) Vol.25 No.5
Ultraviolet (UV) irradiation is a relevant environment factor to induce cellular senescence and photoaging. Both autophagy- and silent information regulator T1 (SIRT1)-dependent pathways are critical cellular processes of not only maintaining normal cellular functions, but also protecting cellular senescence in skin exposed to UV irradiation. In the present studies, we investigated whether modulation of autophagy induction using a novel synthetic SIRT1 activator, heptasodium hexacarboxymethyl dipeptide-12 (named as Aquatide), suppresses the UVB irradiation-induced skin aging. Treatment with Aquatide directly activates SIRT1 and stimulates autophagy induction in cultured human dermal fibroblasts. Next, we found that Aquatide-mediated activation of SIRT1 increases autophagy induction via deacetylation of forkhead box class O (FOXO) 1. Finally, UVB irradiation-induced cellular senescence measured by SA-β-gal staining was significantly decreased in cells treated with Aquatide in parallel to occurring SIRT1 activation-dependent autophagy. Together, Aquatide modulates autophagy through SIRT1 activation, contributing to suppression of skin aging caused by UV irradiation.
Myungho Lee,You Lim Kim,Mun Sun Maeng,Youn Suk Lee 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
The remaining residue in the package after consuming the food products has recently been a major issue in the food industry due to the food waste or loss, and the recycling difficulty of packaging materials. Most liquid foods can easily adhere to the inner surface of the container and remain it leading to food-wasting. Superhydrophobic (SHPC) surface of the package may reduce the remaining portion of the residual liquid food. Surface coating on the package with waxes as the food safety compounds is an eco-friendly treatment of low surface energy hydrocarbons for providing SHPC properties. However, use of only waxes for the surface coating is limited due to the low adhesive forces and thermally unstable for hot food application. This study focuses on the development of liquid food sticky resistance polymeric film with SHPC properties. The coating package structure is fabricated with waxes and silica aerogel via spraying method on LLDPE film. SHPC property of the fabricated film is evaluated using static contact angle and durability test. The results show that the aerogel/wax coated films represent good SHPC properties which have up to 150o contact angle compared to the control.
Neuroprotective effects of Korean White ginseng and Red ginseng in an ischemic stroke mouse model
Myungho Jin,Kyung-Min Kim,Chiyeon Lim,Suin Cho,Young Kyun Kim 고려인삼학회 2022 Journal of Ginseng Research Vol.46 No.2
Background: Stroke is a neurological disorder characterized by brain tissue damage following a decrease in oxygen supply to brain due to blocked blood vessels. Reportedly, 80% of all stroke cases are classified as cerebral infarction, and the incidence rate of this condition increases with age. Herein, we compared the efficacies of Korean White ginseng (WG) and Korean Red Ginseng (RG) extracts (WGex and RGex, respectively) in an ischemic stroke mouse model and confirmed the underlying mechanisms of action. Methods: Mice were orally administered WGex or RGex 1 h before middle cerebral artery occlusion (MCAO), for 2 h; the size of the infarct area was measured 24 h after MCAO induction. Then, the neurological deficit score was evaluated and the efficacies of the two extracts were compared. Finally, their mechanisms of action were confirmed with tissue staining and protein quantification. Results: In the MCAO-induced ischemic stroke mouse model, WGex and RGex showed neuroprotective effects in the cortical region, with RGex demonstrating superior efficacy than WGex. Ginsenoside Rg1, a representative indicator substance, was not involved in mediating the effects of WGex and RGex. Conclusion: WGex and RGex could alleviate the brain injury caused by ischemia/reperfusion, with RGex showing a more potent effect. At 1,000 mg/kg body weight, only RGex reduced cerebral infarction and edema, and both anti-inflammatory and anti-apoptotic pathways were involved in mediating these effects.
AQUATIDE Plays a Role as Innovative Skin-Care Vaccine Through The Autophagy Induction
( Chaejin Lim ),( Myungho Kor ),( Seokjeong Yoon ),( Heungjae Kim ),( Kyungho Park ),( Keedon Park ) 한국피부장벽학회 2016 한국피부장벽학회지 Vol.18 No.2
Natural moisturizing factor (NMF) in the stratum corneum has a high moisture retaining efficacy, and plays a major role in the skin barrier function. Pyrrolidone carboxylic acid (PCA) is one of the major NMFs found in human skin. Aquatide, a PCA-mimetic peptide, improves the skin barrier function by stimulating filaggrin expression as well as reducing Trans-Epidermal Water Loss (TEWL) correlated with increased water retention and moisturization. Autophagy is the natural destructive mechanism that allows the orderly degradation and recycling of cellular damaged components. So, Increased autophagy delays ageing process and extends longevity. Sirtuin1 (SIRT1) is a nicotinamide adenine dinucleotide (NAD+)-dependent deacetylase functioning in the regulation of metabolism, cell survival and organismal lifespan. AQUATIDE alleviates ROS- and heavy metal-mediated senescence and cytotoxicity of cells by SIRT1-dependent autophagy. Furthermore, AQUATIDE reduces pollen-mediated PGE2 release and keeps the skin healthy during an allergic reaction against pollen treatment. In conclusion, AQUATIDE is considered as the first needle-free “skin-care vaccine” for various troubled skins including inflamed and irritated skin as well as aged skin.
Kwon, JiYoon,Lim, SungSam,Baek, SeungHo,Bae, KwangShik,Kang, MyungHoe,Lee, WooCheol 대한치과보존학회 2007 Restorative Dentistry & Endodontics Vol.32 No.3
이 연구의 목적은 치주인대 섬유아세포에 MTA를 접촉시킨 뒤 성장인자 transforming growth factor-betal (TGF-β1), fibroblast growth factor-2 (FGF-2) 및 cytokine interleukin-6 (IL-6)의 발현량 변화를 측정하는 것이다. MTA군에서는 100 mg씩의 ProRoot MTA와 증류수를 혼합하고, IRM군은 동량의 IRM 분말을 용액에 혼합하여, 이 시료들을 경화반응이 진행되도록 7일간 놓아두었다. 사람의 치주인대 섬유아세포를 배양하여 MTA와 TRM 시료 상에 well당 1×10^(5)개 수준으로 도포한 뒤 6, 12, 24, 48시간 동안 배양하였다 (n = 5). 대조군으로는 재료의 접촉 없이 배양한 세포를 사용하였다. 시료에서 상c층액을 분리하여 TGF-β1, FCF-2, IL-6의 발현량을 enzyme-linked immunosorbent assay (ELISA)법으로 측정하였다. MTA군에서, 성장인자인 TGF-β1과 FGF-2는 대조군에 비해 유의성 있게 발현이 억제되었으며 (p<0.05), cytokine인 IL-6 발현량은 대조군과 유사한 수준으로 나타났다. Mineral trioxide aggregate (MTA) would influence healing of periapical tissues by modulating the production of growth factors and cytokines from PDL fibroblasts, however, the studies are insufficient. Therefore, the purpose of this study was to monitor the expression of transforming growth factor-betal (TGF-β1), fibroblast growth factor-2 (FGF-2), and interleukin-6 (IL-6) from PDL fibroblasts in the presence of MTA. The human PDL fibroblasts were seeded onto the set MTA or IRM at a level of 1×10^(5) cells per unit well, and further incubated for 6, 12, 24, and 48 hours. The levels of TGF-β1, FGF-2, and IL-6 from the supernatant were measured by enzyme-linked immunosorbent assay (ELISA). The data were analyzed using one-way ANOVA. The level of TGF-β1 was down-regulated when the cells were grown in the presence of MTA except at 6 hours. The levels of FGF-2 release were significantly suppressed when PDL fibroblasts were grown in the presence of MTA or IRM at all time intervals (p<0.05). The expressions of IL-6 from MTA treated cells were comparable to those of untreated control cells throughout the observation periods. We presume that this material inhibits the stimulatory function of growth factors on granulation tissue formation and in turn, it promotes the healing process modulated by other bone-remodeling cells. [J Kor Acad Cons Dent 32(3):191-197, 2007]