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Seo, Moon-Hyeong,Lee, Tae-Sun,Kim, Eunkyung,Cho, Young Lag,Park, Hee-Sung,Yoon, Tae-Young,Kim, Hak-Sung American Chemical Society 2011 ANALYTICAL CHEMISTRY - Vol.83 No.23
<P>Single-molecule fluorescence resonance energy transfer (smFRET) measurement provides a unique and powerful approach to understand complex biological processes including conformational and structural dynamics of individual biomolecules. For effective smFRET analysis of protein, site-specific dual-labeling with two fluorophores as an energy donor and an acceptor is crucial. Here we demonstrate that site-specific dual-labeling of protein via incorporation of unnatural amino acid provides a clearer picture for the folded and unfolded states of the protein in smFRET analysis than conventional labeling using double cysteines. As a model study, maltose-binding protein (MBP) was dually labeled via incorporation of ρ-azido-<SMALL>l</SMALL>-phenylalanine and cysteine at specific positions, immobilized on a surface, and subjected to smFRET analysis under native and denaturing conditions. The resulting histograms show that site-specific dual-labeling results in a more homogeneous distribution in protein populations, enabling a precise smFRET analysis of protein.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2011/ancham.2011.83.issue-23/ac202096t/production/images/medium/ac-2011-02096t_0004.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac202096t'>ACS Electronic Supporting Info</A></P>
Seo, Moon-Hyeong,Han, Jieun,Jin, Zongwen,Lee, Dong-Won,Park, Hee-Sung,Kim, Hak-Sung American Chemical Society 2011 ANALYTICAL CHEMISTRY - Vol.83 No.8
<P>Immobilization of proteins in a functionally active form and proper orientation is crucial for effective surface-based analysis of proteins. Here we present a general method for controlled and oriented immobilization of protein by site-specific incorporation of unnatural amino acid and click chemistry. The utility and potential of this method was demonstrated by applying it to the analysis of interaction between a pathogenic protein DrrA of <I>Legionella pneumophila</I> and its binding partner Rab1 of human. Kinetic analysis of Rab1 binding onto the DrrA-immobilized surfaces using surface plasmon resonance revealed that immobilization of site-specifically biotinylated DrrA results in about 10-fold higher sensitivity in binding assay than the conventional immobilization of DrrA with random orientation. The present method is expected to find wide applications in the fields of the surface-based studies of protein−protein (or ligand) interactions, drug screening, biochip, and single molecule analysis.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2011/ancham.2011.83.issue-8/ac103334b/production/images/medium/ac-2010-03334b_0002.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac103334b'>ACS Electronic Supporting Info</A></P>
구형서(Hyeong-Seo Koo),윤형석(Hyeong-Seok Yoon),김진홍(Jin-Hong Kim),유양우(Yang-Woo Yu),문남두(Nam-Doo Moon),이명준(Myung-Joon Lee) 한국정보과학회 2001 한국정보과학회 학술발표논문집 Vol.28 No.2Ⅲ
Jini는 분산 컴퓨팅 환경에서 네트워크 플러그 앤 워크(Network Plug and Work)를 가능하게 해주는 기술로서, 실세계의 네트워크에 연결된 주변기기나 응용프로그램을 Jini 서비스로 등록하여 동적으로 작업환경을 구성할 수 있는 새로운 분산 패러다임으로 자리잡고 있다. 본 논문에서는 Jini 기반의 이동 에이전트 시스템을 설계하고 구현하였다. SMART, Grasshopper, Aglets같은 CORBA 기반의 이동 에이전트 시스템은 표준화, 동적 서비스 등록 및 에이전트의 영속성을 지원하기에 미흡하였으나, 개발된 시스템은 Jini 서비스를 이용하여 이러한 문제들을 체계적으로 해결하고자 하였다. 본 시스템은 Jini 서비스로 동작하는 이동 에이전트 시스템, 에이전트와 에이전트 시스템의 위치정보를 관리하는 위치정보제공자, 그리고 이동 불가능한 에이전트를 임시 저장하는 이동경로관리자로 구성된다.
Seo, Chang Ho,Ju, Jeong Il,Kim, Mi-Hyeong,Jun, Kang Woong,Ahn, Sang-Hyun,Hwang, Jeong Kye,Kim, Sang Dong,Park, Sun Cheol,Choi, Bum Soon,Kim, Ji Il,Yang, Chul Woo,Kim, Yong Soo,Moon, In Sung The Korean Surgical Society 2015 Annals of Surgical Treatment and Research(ASRT) Vol.89 No.4
<P><B>Purpose</B></P><P>The purpose of this study was to analyze the risk factors for delayed graft function (DGF) and determine its impact on the outcomes of deceased donor (DD) kidney transplantation (KT).</P><P><B>Methods</B></P><P>Between January 2000 and December 2011, we performed 195 DD renal transplants. After the exclusion of primary nonfunctional grafts (n = 4), the study recipients were divided into two groups-group I, DGF (n = 31, 16.2%); group II, non-DGF (n = 160, 83.8%). The following variables were compared: donor and recipient characteristics, patient and graft survival, postoperative renal function, acute rejection (AR) episodes, and the rates of surgical and infectious complications.</P><P><B>Results</B></P><P>Donor-related variables that showed significant differences included hypertension (P = 0.042), diabetes (P = 0.025), and prerecovery serum creatinine levels (P < 0.001). However, there were no significant differences in recipient-related factors. One significantly different transplant-related factor was positive panel reactive antibody (PRA > 20%, P = 0.008). On multivariate analysis, only the prerecovery serum creatinine level (P < 0.001; hazard ratio [HR], 1.814) was an independent risk factor for the development of DGF. A Cox multivariate analysis of risk factors for graft survival identified these independent risk factors for graft survival: nephron mass (donor kidney weight to recipient body weight ratio) index (P = 0.026; HR, 2.328), CMV infection (P = 0.038; HR, 0.114), and AR episode (P = 0.038; HR, 0.166).</P><P><B>Conclusion</B></P><P>In DD KT, an independent risk factor for DGF was the prerecovery serum creatinine level. Although there was a significant difference in graft survival between the DGF and non-DGF groups, DGF was not an independent risk factor for graft failure in this study.</P>
Seo, Hae-Moon,Park, Yong-Kuk,Park, Woo-Chool,Kim, Dong-Su,Lee, Myung-Soo,Kim, Hyeong-Seok,Choi, Pyung Korean Society for Internet Information 2008 KSII Transactions on Internet and Information Syst Vol.2 No.1
At the present time the task of designing a highly integrated ZigBee radio frequency (RF) receiver with an excellent coexistence performance is still very demanding and challenging. This paper presents a number of system issues and design considerations for a ZigBee RF receiver, namely IEEE 802.15.4, for coexistence with wireless devices in the 2.4-GHz ISM-band. With regard to IEEE 802.15.4, the paper analyzes receiver performance requirements for; system noise figure (NF), system third-order intercept point (system-IIP3), local oscillator phase noise and selectivity. Based on some assumptions, the paper illustrates the relationship between minimum detectable signal (MDS) and various situations that involve the effects of electromagnetic interference generated by other wireless devices. We infer the necessity of much more stringent specification requirements than the published standard for various wireless communication field environments