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Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression
Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11
<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>
( Yoon Ho Jang ),( Yoon Sik Kim ),( Sung Wook Chun ),( Ye Ju Lee ),( Mi Ok Kim ),( Eur Soon Kim ),( Byeoung Gu Kim ),( Sang Keun Shin ) 한국체육학회 2016 국제스포츠과학 학술대회 Vol.2016 No.1
Purpose: This research aimed to verify the effects of a 32-week low-intensity combined exercise program as an exercise intervention on body composition and functional fitness in elderly women. Method: A total of 24 subjects were assigned to Otago exercise group(n=12) and control group(n=12) randomly. A 60 minute Otago exercise was applied to them as the intervention 3 times a week for 32 weeks by 20-25%HRR. Exercise Intensity was set by using Polar and %HRR, with participants’ heart rates monitored. Body composition was measured and calculated to compare the effects of exercise on the baseline and 32 weeks later. Items for body composition contained height, weight, BMI, WHR, %fat, %LBM, Fat, LBM, fat/height, and LBM/height. Functional fitness was measured by Senior Fitness Test. Mean and standard deviations were calculated to analyze the research data by using SPSS Version 22.0. A two-way repeated measures ANOVA was conducted to verify interactions between pre-post measurement and groups. Statistical significance was set at p<.05. Result: This research showed that no significant interactions between pre-post measurement and groups appeared in any body composition items for Otago exercise group. It showed a significant increase(p<.05) between pre-post measurements in WHR for Otago exercise group and between groups. There were significant interactions between time and groups in 30-second chair stand(p<.01), 30-second arm curl(p<.05), back scratch(p<.01) and 8-foot up-and-go(p<.05) for Otago exercise group. Significant increase appeared between groups in 30-second chair stand(p<.01), and 30-second arm curl(p<.001) for Otago exercise group. Significant increase appeared between time in 30-second chair stand(p<.05), and back scratch(p<.05) for Otago exercise group. Conclusion: Although 32-week low-intensity combined exercise had no effects on body composition in elderly women, it verified the significant decrease between pre-post measurements of WHR for exercise group. And this exercise program showed the effects on low body strength, upper body strength, upper body flexibility, dynamic balance and agility for exercise group in elderly women. In spite of low-intensity exercise by 20-25%HRR, this program can be used as an effective and safe exercise intervention to improve strength and prevent falls for elderly women living in a community.
Yoon, Il,Seo, Joobeom,Lee, Ji-Eun,Song, Mi Ryoung,Lee, So Young,Choi, Kyu Seong,Jung, Ok-Sang,Park, Ki-Min,Lee, Shim Sung Royal Society of Chemistry 2005 Dalton Transactions Vol.2005 No.14
<P>By virtue of simple one donor variations of O<SUB>2</SUB>S<SUB>2</SUB>X-donor macrocycles (L<SUP>1</SUP>: X&z.dbd;S, L<SUP>2</SUP>: X&z.dbd;O and L<SUP>3</SUP>: X&z.dbd;NH) towards silver(<SMALL>I</SMALL>) perchlorates, four supramolecular complexes (1–3) with different topologies have been isolated; L<SUP>1</SUP> afforded solvent- and anion-free sandwich complex (1), otherwise L<SUP>2</SUP> and L<SUP>3</SUP> gave the dimeric (2a), 1-D polymeric (2b), and tetrameric bowl-type (3) complexes with solvent or anion coordination.</P> <P>Graphic Abstract</P><P>By virtue of simple one donor variations of O<SUB>2</SUB>S<SUB>2</SUB>X-donor macrocycles (L<SUP>1</SUP>: X&z.dbd;S, L<SUP>2</SUP>: X&z.dbd;O and L<SUP>3</SUP>: X&z.dbd;NH) towards silver(<SMALL>I</SMALL>) perchlorates, four supramolecular complexes (1–3) with different topologies have been isolated. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b506438d'> </P>
Direct Control of Stem Cell Behavior Using Biomaterials and Genetic Factors
Yoon, Jeong-Kee,Kang, Mi-Lan,Park, Joo Hyun,Lee, Kyoung-Mi,Shin, Young Min,Lee, Jin Woo,Kim, Hyun Ok,Sung, Hak-Joon Hindawi 2018 Stem cells international Vol.2018 No.-
<P>Stem cells have recently emerged as an important candidate for cell therapy. However, some major limitations still exist such as a small quantity of cell supply, senescence, and insufficient differentiation efficiency. Therefore, there is an unmet need to control stem cell behavior for better clinical performance. Since native microenvironment factors including stem cell niche, genetic factors, and growth factors direct stem cell fate cooperatively, user-specified <I>in vitro</I> settings are required to understand the regulatory roles and effects of each factor, thereby applying the factors for improved cell therapy. Among others, various types of biomaterials and transfection method have been employed as key tools for development of the <I>in vitro</I> settings. This review focuses on the current strategies to improve stemness maintenance, direct differentiation, and reprogramming using biomaterials and genetic factors without any aids from additional biochemicals and growth factors.</P>
( Mi Jung Shin ),( Byung Soo Kim ),( Young Ok Kim ),( Ho Cheol Song ),( Yong Soo Kim ),( Suk Young Kim ),( Euy Jin Choi ),( Yoon Sik Chang ),( Byung Kee Bang ) 대한신장학회 2008 Kidney Research and Clinical Practice Vol.27 No.1
Purpose: Cardiovascular disease is one of the leading causes of mortality and morbidity in hemodialysis patients. Arterial stiffness is known to be associated with vascular calcification in HD patients. Post-dialysis hypotension is a risk factor for cardiovascular mortality. We evaluated the relation between arterial stiffness and post-dialysis blood pressure in maintained HD patients. Methods: 72 HD patients were enrolled in this study. They had been under maintenance HD for more than 3 months. We checked the biochemical data, including the troponin T, CRP and OPG (osteoprotegerin) levels before their dialysis session and the baPWV (brachio-ankle pulse wave velocity) after the session. We defined post-dialysis BP decrease as a drop of BP of more than 5% of the average MAP for 2 weeks (6 sessions). Results: There were 34 and 38 patients with and without post-dialysis BP decrease, respectively. The BP decrease group had higher CRP, troponin T and OPG levels (p<0.05, 0.01 and 0.01, respectively). The PWV was higher in the BP decrease group (p<0.001). The CRP and troponin T levels were positively correlated with the PWV (r=0.26, p<0.05; r=0.31, p<0.01, respectively). The OPG level was positively correlated with the PWV (r=0.44, p<0.001). Age, pre-dialysis pulse pressure and drops in the patients MAP were correlated with the PWV (r=0.33, p<0.05; r=0.31, p<0.05; r=0.30, p<0.05, respectively). On multivariate analysis, PWV was the independent factor related to the drops in the MAP of the patients (ß=0.311, p=0.021). Conclusion: Arterial stiffness is associated with post-dialysis blood pressure decrease.