Expression, phosphorylation, localization, and microtubule binding of tau in colorectal cell lines

HUDA MD NAZMUL,김다혜,ERDENE OCHIR ERDENEDOLGOR,김윤석,판철호 한국응용생명화학회 2016 Applied Biological Chemistry (Appl Biol Chem) Vol.59 No.6

Tau is a microtubule-associated protein that causes proper role of neuron by assembling and stabilizing microtubules. The amount and post translational modification of tau can change its function as a stabilizer of microtubules. The aim of the study was to look for the expression of tau in colorectal cancer and normal cells, along with phosphorylation and microtubule binding properties of tau expressed in colorectal cancer cell. Two colorectal cancer cells (SW480 and HCT 116) expressed tau that was also phosphorylated, whereas the others (Caco-2, HT-29 and DLD1) did not. Colorectal normal cell (CCD-18Co) expressed very tiny amount of tau that was not phosphorylated. A big fraction of tau in HCT 116 did not bind to microtubule. The results suggest that some colorectal cancer cells express hyperphosphorylated tau as found in Alzheimer’s disease. So, tau in colorectal cancer cells does not look like same as tau in normal adult brain; rather it works nearly same as tau in neurodegenerative disease.

Palm oil industry’s bi-products as coarse aggregate in structural lightweight concrete

Md. Nazmul Huda,Mohd Zamin Jumaat,A. B. M. Saiful Islam,Kh Mahfuz ud Darain,M. Obaydullah,Md. Akter Hosen 사단법인 한국계산역학회 2017 Computers and Concrete, An International Journal Vol.19 No.5

Recent trend is to use the lightweight concrete in the construction industry because it has several advantages over normal weight concrete. The Lightweight concrete can be produced from the industrial waste materials. In South East Asian region, researchers are very keen to use the waste materials such as oil palm shell (OPS) and palm oil clinker (POC) from the palm oil producing industries. Extensive research has been done on lightweight concrete using OPS or POC over the last three decades. In this paper the aggregate properties of OPS and POC are plotted in conjunction with mechanical and structural behavior of OPS concrete (OPSC) and POC concrete (POCC). Recent investigation on the use of crushed OPS shows that OPSC can be produced to medium and high strength concrete. The density of OPSC and POCC is around 20-25% lower than normal weight concrete. Generally, mechanical properties of OPSC and POCC are comparable with other types of lightweight aggregate concrete. It can be concluded from the previous study that OPSC and POCC have the noteworthy potential as a structural lightweight concrete.

Eliminating concrete cover separation of NSM strengthened beams by CFRP end anchorage

Md. Akter Hosen,Mohd Zamin Jumaat,A. B. M. Saiful Islam,Mohamed Kamruzzaman,Md. Nazmul Huda,Mahmudur Rahman Soeb 국제구조공학회 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.56 No.6

Upgrading or strengthening of existing reinforced concrete (RC) infrastructure is an emerging demand nowadays. Near Surface Mounted (NSM) technique is very promising approach for flexural strengthening of RC members. However, premature failure such as concrete cover separation failure have been a main concern in utilizing this technique. In this study, U-wrap end anchorage with carbon fiber reinforced polymer (CFRP) fabrics is proposed to eliminate the concrete cover separation failure. Experimental programs were conducted to the consequence of U-wrap end anchorage on the flexurally strengthened RC beams with NSM- steel. A total of eight RC rectangular beam specimens were tested. One specimen was kept unstrengthened as a reference; three specimens were strengthened with NSM-steel bars and the remaining four specimens were strengthened with NSM-steel bars and U-wrap end anchorage using CFRP fabrics. A 3D non-linear finite element model (FEM) was developed to simulate the flexural response of the tested specimens. It is revealed that NSM-steel (with and without end-anchors) significantly improved the flexural strength; moreover decreased deflection and strains compared with reference specimen. Furthermore, NSM-steel with end anchorage strengthened specimens revealed the greater flexural strength and improve failure modes (premature to flexure) compared with the NSM-steel without end anchorage specimens. The results also ensured that the U-wrap end anchorage completely eliminate the concrete cover separation failure.

Cloning of a novel endogenous promoter for foreign gene expression in Phaeodactylum tricornutum

ERDENE OCHIR ERDENEDOLGOR,신복규,HUDA MD NAZMUL,김다혜,이은하,송대근,김윤미,김상민,판철호 한국응용생명화학회 2016 Applied Biological Chemistry (Appl Biol Chem) Vol.59 No.6

Phaeodactylum tricornutum is a model diatom, and its genomic sequence data and expressed sequence tag databases are available. This study was to discover a new endogenous promoter that drives strong constitutive expression of a protein of interest in P. tricornutum. To find promoter candidates, the intracellular proteins of P. tricornutum grown to stationary phase were extracted and identified by LC–MS/MS. Glutamine synthetase (GLNA) was one of the most abundantly expressed proteins during the stationary phase. Promoter is usually located on 5′ upstream region of open reading frame of the gene. Thus, two fragments of 5′ upstream region of the GLNA gene as putative promoters, 501 and 997 bp long, were amplified and cloned into enhanced Green Fluorescent Protein (eGFP) reporter systems. The constructed reporter systems were transformed into P. tricornutum and the eGFP expression levels were compared to those of reporter systems using the promoters of fcpA (fucoxanthin chlorophyll a/c binding protein A) and CIP1 (putative replication-associated proteins of a Chaetoceros lorenzianus-infecting DNA virus) as controls. The expression of eGFP driven by either GLNA promoter (501 and 997 bp) was linearly related to cell density, and eGFP was expressed constitutively regardless of the cultivation phase. The eGFP expression level driven by the GLNA promoters was at least 4 times higher than the fcpA-driven eGFP expression level at the stationary phase. The 501 and 997 bp regions of the GLNA promoter had similar activity patterns for transcribing the downstream gene. These results indicate that at least 501-bp region of the GLNA promoter can be used as a strong constitutive promoter in genetic engineering of P. tricornutum.

Incorporation preference for rubber-steel bearing isolation in retrofitting existing multi storied building

A.B.M. Saiful Islam,University of MalayaMohd Zamin Jumaat,Raja Rizwan Hussain,Md. Akter Hosen,Md. Nazmul Huda 사단법인 한국계산역학회 2015 Computers and Concrete, An International Journal Vol.16 No.4

Traditionally, multi-story buildings are designed to provide stiffer structural support to withstand lateral earthquake loading. Introducing flexible elements at the base of a structure and providing sufficient damping is an alternative way to mitigate seismic hazards. These features can be achieved with a device known as an isolator. This paper covers the design of base isolators for multi-story buildings in medium-risk seismicity regions and evaluates the structural responses of such isolators. The well-known tower building for police personnel built in Dhaka, Bangladesh by the Public Works Department (PWD) has been used as a case study to justify the viability of incorporating base isolators. The objective of this research was to establish a simplified model of the building that can be effectively used for dynamic analysis, to evaluate the structural status, and to suggest an alternative option to handle the lateral seismic load. A finite element model was incorporated to understand the structural responses. Rubber-steel bearing (RSB) isolators such as Lead rubber bearing (LRB) and high damping rubber bearing (HDRB) were used in the model to insert an isolator link element in the structural base. The nonlinearities of rubber-steel bearings were considered in detail. Linear static, linear dynamic, and nonlinear dynamic analyses were performed for both fixed-based (FB) and base isolated (BI) buildings considering the earthquake accelerograms, histories, and response spectra of the geological sites. Both the time-domain and frequency-domain approaches were used for dynamic solutions. The results indicated that for existing multi-story buildings, RSB diminishes the muscular amount of structural response compared to conventional non-isolated structures. The device also allows for higher horizontal displacement and greater structural flexibility. The suggested isolation technique is able to mitigate the structural hazard under even strong earthquake vulnerability.

Establishment of Patient-Derived Gastric Cancer Organoid Model From Tissue Obtained by Endoscopic Biopsies

Song Hana,Park Jae Yong,Kim Ju-Hee,Shin Tae-Seop,Hong Soon Auck,Huda Md Nazmul,Kim Beom Jin,Kim Jae Gyu 대한의학회 2022 Journal of Korean medical science Vol.37 No.28

Cancer organoids are three-dimensional mini-organ analogues derived from cancer tissues and have been proposed as models capable of simulating the structure and function of human organs and tissues in vitro. We sought to establish gastric cancer patient-derived organoids (PDOs) from tissues obtained by endoscopic biopsies. Gastric cancer-PDOs were successfully established and cultured from cancer tissues with gastric adenocarcinoma by endoscopic biopsies. To confirm that gastric cancer-PDOs were derived from cancer tissue, the consistency of the original cancer tissue was assessed by histopathological examination. As a result, it was confirmed that the shape and internal structure of gastric cancer-PDO were derived from the original gastric cancer cells, and the tumor specificity of gastric cancerPDO was confirmed through Periodic acid-Schiff (PAS) and polyclonal carcinoembryonic antigen antibody staining. These results demonstrate that gastric cancer-PDO models show the characteristics of primary tumors and have potential for drug screening and providing a personalized medicine platform.