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판철호,김은선,엄병헌,이재권 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.4
Flavonoids have biological activities including anti-allergic, anti-inflammatory, antimicrobial and anticancer activities shown from in vitro studies. Of these biological activities, the antiinflammatory capacity of flavonoids has long been emphasized in Chinese medicine. In this study, I investigated that what flavonoid can be applied to the suppression of lipopolysaccharide (LPS)-induced inflammatory responses in macrophages among the four similar structure flavonoids. Eriodictyol was found to reduce nitric oxide (NO) production from LPS-stimulated Raw 264.7 cells in non-cytotoxic concentrations. Moreover, eriodictyol strongly suppressed the phagocytic activity of activated macrophages. Pre-treatment of Raw 264.7 cells with eriodictyol reduced the expression of mRNA and the secretion of pro-inflammatory cytokines. These inhibitory effects were found to be caused by blockage of nuclear factor kappa-light-chainenhancer of activated B cells (NF-κB) activation and phosphorylation of p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinases 1 and 2 (ERK1/2) and c-Jun Nterminal kinase (JNK).
Tectorigenin Inhibits IFN-γ/LPS-induced Inflammatory Responses in Murine Macrophage RAW 264.7 Cells
판철호,김은선,정상훈,노주원,이재권 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.11
Tectorigenin (Tg) and tectoridin (Td) are the major compounds isolated from the rhizomes of iridaceous plant Belamcanda chinensis which is well known as a chinese traditional medicine for the treatment of inflammatory diseases. In this study we investigated whether tectorigenin and tectoridin can be applied to the suppression of interferon-γ and lipopolysaccharide (IFN-γ/LPS)-induced inflammatory responses in macrophages. Anti-inflammatory activities of tectorigenin and tectoridin were compared with genistein (Ge), well known isoflavonoid as a phytoestrogen and regarded as an emerging anti-inflammatory agent. Both compounds showed low cytotoxic effect. In Raw 264.7 cells activated with IFN-γ/LPS, pre-treated tectorigenin was found to inhibit the expression of inducible nitric oxide synthase (iNOS), the production of nitric oxide (NO) and the secretion of interleukin (IL)-1β dose-dependently. Tectorigenin also decreased the expression of cyclooxigenase (COX)-2 and the production of prostaglandin E2 (PGE₂) in dose-dependent manner. These inhibitory effects of tectorigenin were found to be caused by the blocking of nuclear factor kappa-B (NF-κB) activation. Compared with genistein and tectoridin, tectorigenin showed significant inhibitory effect for almost anti-inflammatory tests in this study. All these results clearly demonstrated that tectorigenin appears to have the potential to prevent inflammation.
Study on the materialization of microalgal carotenoid
판철호 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.0
Microalgae are important primary producers in aquatic environments and play a critical role in supporting aquatic animals. Various chemicals, including fatty acids, sterols, phenolic compounds, terpenes, enzymes, polysaccharides, alkaloids, toxins, and pigments, have been isolated and structurally determined from an extensive investigation of microalgae. Carotenoids are biosynthesized by bacteria, algae, fungi, and plants, but not by animals, which must obtain carotenoids from their food. In this study, we focused on the materialization of carotenoids from Chlorella sp. and marine diatoms.
Enzymatic Properties of Recombinant Rehmannia Class III Chitinase Expressed in Bacillus subtilis
판철호,Su-Il Kim 한국키틴키토산학회 2008 한국키틴키토산학회지 Vol.13 No.3
To investigate the enzymatic properties of an acidic class III chitinase of which gene, JHIII, was isolated from Rehmannia glutinosa, the recombinant protein, rJHII, was obtained from the culture of B. subtilis WB600 transformed with JHIII gene. It showed a maximum chitinolytic activity at pH 6 and 50oC, and revealed a relatively high enzymatic activity of more than 50% in the broad range of pH (pH 2 to pH 8) and temperature (20oC to 70oC). The catalytic activity of rJHI was lowered to 85% and 42% in the presence of 5M and 10 M allosamidin, respectively, which is a potent competitive inhibitor of family 18 chitinases. The rJHIII was considered as an endo-type enzyme because it hydrolyzed (GlcNAc)6 into (GlcNAc)₂, (GlcNAc)₃, and (GlcNAc)₄, but did not hydrolyze (GlcNAc)₂-5. The rJHI inhibited the growth of Fusarium oxysporum R2, which was isolated from the root of Rehmannia and identified as a specific fungal pathogen of Rehmannia. The inhibition by rJHIII was a dose-dependent manner.
한국 자생식물 추출물의 항산화 및 Aldo-keto Reductase Family 1 B10 효소 억제 효과
판철호 ( Cheol Ho Pan ),이주영 ( Joo Young Lee ),송대근 ( Dae Geun Song ),김종환 ( Jong Hwan Kim ),안수용 ( Soo Young Ahn ),배덕성 ( Deok Sung Bae ),김영훈 ( Young Hoon Kim ),이재권 ( Jae Kwon Lee ) 한국응용생명화학회 2009 Journal of Applied Biological Chemistry (J. Appl. Vol.52 No.4
판철호(Cheol Ho Pan),이은아(Eun A Lee),조도현(Do Hyun Jo),김수일(Su Il Kim) 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.7
Chitinase, which is one of the pathogenesis-related proteins, was examined from Rehmannia glutinosa. Rehmannia chitinases were classified into basic and acidic groups by chitin affinity column chromatography. According to the chitinase pattern by native PAGE, basic chitinases of low Rf values were extracted more under an acidic condition (pH 2.9) than a basic condition (pH 8.8), while acidic chitinases having high Rf values were mainly detected in basic extracts. There were in total seven chitinase isoforms of three basic and four acidic isoforms in Rehmannia. The range of molecular weight of Rehmannia chitinase was from 28 to 32 kD. It showed the possibility of tissue specific expression of acidic chitinases and basic chitinases were mainly detected in root.
정화빈,판철호,윤원병 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.5
The effect of particle size on the extraction rate of anthocyanin from Solanum tuberosum L. (purple-fleshed potato) peels (PFPPs) using the stagnant single-stage batch extraction system was investigated. Core pretreatment processes such as drying and grinding were quantitatively evaluated using mathematical models. The drying behavior of PFPP was successfully described using thin layer models (Page model and Midilli-Kucuk model). The effective diffusion coefficient of drying temperature at 40oC was determined to be 1.67x10−12 m2/s. The grinding time to obtain particles of a specific size was accurately estimated using the grinding kinetic model (R2=0.97). The extraction rate of anthocyanin increased as the particle size decreased; however, when the particle size was 0.15mm, the anthocyanin content decreased. Our study demonstrated that the grinding kinetic model is useful to estimate the grinding time to produce an optimum particle size for anthocyanin extraction from PFPP.
고초균에서 발현된 벼 키틴분해효소 ( CH16 ) 의 병원성 곰팡이 생장 저해 효과
손종문(Jong Mun Son),판철호(Cheol Ho Pan),소민영(Min Young So),채영암(Young Am Chae),김수일(Su Il Kim) 한국키틴키토산학회 2001 한국키틴키토산학회지 Vol.6 No.1
N/A To investigate whether or not translational product of rice chitinase cDNA CH16 has antifungal activity, recombinant CH16 was produced in microorganism and its antifungal activity was tested in vitro. The rice chitinase cDNA, CH16, which encodes a basic class I chitinase, was expressed in Bacillus subtilis. To prepare the expression plasmid, CH16 was inserted into the multicloning site of a plasmid, p8A-1, which contains the promoter and signal sequence of Bacillus amylase. The expression cassette consisting of promoter, signal sequence, and ORF of CH16 was subcloned into the shuttle vector, pUBL140, which can be replicated in E. coli as well as B. subtilis. The resulting plasmid was named as pUBLCH16. Expression plasmid was transformed in B. subtilis WB600 deficient in six extracellular proteases and transformant expressed recombinant chitinase, CH16, at the stationary phase. CH16 protein showed the growth inhibition of Fusarium oysporum R2 which was isolated from the root of Rhemannia glutinosa and specifically infected to Rehmannia root.