Evidence of Changes in Cell Surface Proteins During Growth of Lactobacillus casei under Acidic Conditions

Marzieh Hosseini Nezhad,Matthew Knight,Margaret Lorraine Britz 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.1

In this research, changes were observed in cell surface proteins of a typical strain of the Lactobacillus casei was investigated in response to acidic growth conditions. Two dimensional electrophoresis and Western blot analyses were carried out to detect changes in relative abundance of proteins at the cell surface. The identity of the differentially expressed proteins extracted by LiCl, a chemical routinely used to extract surface proteins of lactic acid bacteria, was determined by MALDI-TOF/TOF MS. Many enzymes involved in glycolysis were up-regulated in the cell surface fraction following growth at low pH, including enolase,lactate dehydrogenase, and glyceraldehyde-3-phosphate dehydrogenase. Several of these proteins were also related to adhesion and generalized stress responses. It is demonstrated that growth of L. casei under acidic conditions caused molecular changes at the cell surface to develop an adaptive strategy corresponding to slower growth at low pH.

Empowering Rural Housewives in Iran: Utilizing the Transtheoretical Model to Increase Physical Activity

Borhani Mahboobe,Hosseini Zakieh Sadat,Shahabodin Najme,Mehri Ali,Kiani Mohadese,Abedi Marzieh 대한예방의학회 2024 예방의학회지 Vol.57 No.2

Objectives: Rural housewives are integral to household management and family care, yet their sedentary lifestyles present significant health risks. This study used the transtheoretical model (TTM) to investigate strategies that encourage and maintain regular exercise habits among rural housewives.Methods: A semi-experimental study was conducted in 2021 with 114 housewives aged 30 to 59 who attended rural health centers in Gorgan, Iran. Participants were randomly assigned to 1 of 2 groups. Data collection involved a validated questionnaire that gathered demographic information and constructs of the TTM. The intervention group participated in a comprehensive educational program, which included four 60-minute sessions. Data were collected again 6 months post-intervention and analyzed using descriptive and inferential statistics in SPSS version 21.Results: The study encompassed women with an average age of 39.75±6.05 years, the majority of whom had educational levels below a diploma, and over 90% were married. We observed strong correlations between the processes of change, self-efficacy, and decisional balance. At the outset, there were no significant differences in demographics or model structures between the 2 groups. However, 6 months post-intervention, the intervention group exhibited statistically significant differences in the mean scores of model structures, stages of change, and body mass index (<>p<0.05).Conclusions: This study highlights the importance of physical activity training for rural housewives. The findings suggest that the educational intervention, which utilized the TTM, significantly impacted the participants’ model structures and their stages of change.

Interleukin-12 and Interleukin-6 Gene Polymorphisms and Risk of Bladder Cancer in the Iranian Population

Ebadi, Nader,Jahed, Marzieh,Mivehchi, Mohamad,Majidizadeh, Tayebeh,Asgary, Mojgan,Hosseini, Seyed Ali Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18

Interleukin-12 (IL-12) as an antitumor and interleukin-6 (IL-6) as an inflammatory cytokine, are immunomodulatory products that play important roles in responses in cancers and inflammation. We tested the association between two polymorphisms of IL-12(1188A>C; rs3212227) and IL-6 (-174 C>G) and the risk of bladder cancer in 261 patients and 251 healthy individuals. We also investigated the possible association of these SNPs in patients with high-risk jobs and smoking habits with the incidence of bladder cancer. The genotype distributions of IL-6 (-174 C/G) genotype were similar between the cases and the control groups; however, among patients with smoking habits, the association between IL-6 gene polymorphism and incidence of bladder cancer was significant. After a control adjustment for age and sex, the following results were recorded: CC genotype (OR= 2.11, 95%CI=1.56-2.87, p=0.007), GC genotype (OR=2.18, 95%CI=1.16-4.12, p=0.014) and GC+CC (OR=2.6, 95%CI=1.43-4.47, p=0.011). A significant risk of bladder cancer was observed for the heterozygous genotype (AC) of IL-12 (OR=1.47, 95%CI=1.01-2.14, p=0.045) in all cases, and among smokers (AC) (OR=3.13, 95%CI=1.82-5.37, p=0.00014), combined AC+CC (OR=3.05, 95%CI=1.8-5.18, p=0.000015). Moreover among high risk job patients, there was more than a 3-fold increased risk of cancer in the carriers of IL-12 beta heterozygous (OR=3.7, 95%CI=2.04-6.57, p=0.000056) and combined AC+CC(OR=3.29, 95%CI=1.58-5.86, p=0.00002) genotypes as compared with the AA genotype with low-risk jobs. As a conclusion, this study suggests that IL-12(3'UTR A>C) and IL-6 (-174 C>G) genotypes are significantly associated with an increased risk of bladder cancer in the Iranian population with smoking habits and/or performing high-risk jobs.

Concentrated O/W emulsions formulated by binary and ternary mixtures of sodium caseinate, xanthan and guar gums: rheological properties, microstructure, and stability

Khadije Abdolmaleki,Leyla Alizadeh,Seyede Marzieh Hosseini,Kooshan Nayebzadeh 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.12

The effects of xanthan gum (XG) (0, 0.3, 0.6wt%), guar gum (GG) (0, 0.3, 0.6 wt%) and XG:GGmixtures (0.3–0.3, 0.3–0.6, 0.6–0.3 and 0.6–0.6 wt%) onthe physical stability of sodium caseinate (CN) stabilizedconcentrated O/W emulsions (uoil = 0.6) were examined. The emulsion stability, microstructure, droplets size distribution,and rheological properties were evaluated. Thefindings showed that with increasing total gum concentrationup to 0.6% droplets size and emulsion instabilitysignificantly decreased (p\0.05). The emulsion containinga ternary mixture of CN:XG:GG at total gum concentration(0.6%) with a mixing ratio of 0.3:0.3 XG:GGexhibited the best stability with the highest ESI value(98.3%). Above the critical concentration, an excessiveincrease in storage modulus led to a significant increase indroplet size and emulsion instability. In brief, concentratedemulsions stabilized by binary and ternary mixtures (CN/XG/GG) may be applicable in special food like heavycream and as a template for fabricating oleogels.

Clinical and Ultrasonographic Changes of the Breast after Use of Soy Isoflavones

Alipour, Sadaf,Afshar, Somayeh,Moini, Ashraf,Dastjerdi, Marzieh Vahid,Saberi, Azin,Bayani, Leila,Eslami, Bita,Hosseini, Ladan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

Background: Phytoestrogens may be an alternative therapy in control of menopausal symptoms but their definite effects on breast tissue must be determined. Our study aimed to define the clinical and ultrasonographic changes of the breast after use of soy isoflavones in menopausal women. Materials and Methods: Menopausal women with hot flashes were randomly grouped as cases and controls and cases received soy isoflavones for 12 weeks. Breast examination (BE) and ultrasonography (US) were done at 0, 6 and 12 weeks. Tenderness and nodularity on BE were graded 1-4 by breast surgeons. Results: There were 30 women in the case and 26 in the control group. The mean age was 51.3 years and the mean age of menopause was 49.2 years. There was no change in the BE and US at 6 weeks in controls. In the case group, 10% had grade 1 tenderness and 13.3% grade 2 tenderness and grade 1 nodularity in BE accompanied with diffuse small cysts in US. At 12 weeks, there was no change in BE and US in the 2 groups. Conclusions: There was no statistically significant difference in the BE of the 2 groups at 6 and 12 weeks (p value=0.36 and 0.41 for nodularity and tenderness respectively) and in the US results. Although the literature contains many facts concerning PEs and the breast, further prospective studies are needed to identify structural breast changes produced by PEs in order to identify the appropriate dosage and indications of use.

Antihyperglycemic and Antioxidant Activity of Crocin in Streptozotocin-Induced Diabetic Rats

Ziba Rajaei,Mousa-Al-Reza Hadjzadeh,Habibollah Nemati,Mahmoud Hosseini,Marzieh Ahmadi,Somayeh Shafiee 한국식품영양과학회 2013 Journal of medicinal food Vol.16 No.3

The aim of the present study was to investigate the antihyperglycemic and protective potential of crocin, a pharmacologically active constituent of Crocus sativus L., in streptozotocin-induced diabetic rats. Rats were administered crocin intraperitoneally at doses of 15, 30, and 60 mg/kg of body weight for 6 weeks. The levels of thiobarbituric acid reactive substance (TBARS) and total thiol (SH) groups were measured in the liver and kidney at the end of 6 weeks. Under our experimental conditions, crocin at a dose of 60 mg/kg was found to significantly reduce the blood glucose level in diabetic animals. In addition, there was a significant increase in TBARS levels and decreased total thiol concentrations in the liver and kidney of diabetic animals. Crocin, at doses of 30 and 60 mg/kg, appears to exert an antioxidative activity demonstrated by a lowering of lipid peroxidation levels in these organs. In conclusion, our findings suggest that crocin has the hypoglycemic and antioxidative properties in streptozotocin-induced diabetes and it may be useful in the management of diabetic patients

Additional cytogenetic aberrations in chronic myeloid leukemia: a single-center experience in the Middle East

Akbar Safaei,Ahmad Monabati,Moeinadin Safavi,Ali Atashabparvar,Marzieh Hosseini 대한혈액학회 2018 Blood Research Vol.53 No.1

BackgroundAdditional cytogenetic aberrations are associated with disease progression in chronicmyeloid leukemia (CML). This study was conducted to determine the type and frequencyof these aberrations and their relationship with hematologic and molecular findings inthe Middle East. MethodsIn this retrospective study, 134 well-established cases of CML were selected from 2010to 2016. Their hematologic phase and type of fusion gene were determined. Finally, theirkaryotypes were analyzed and reported according to ISCN 2013. ResultsPatients had a mean age of 44 years. Twenty-two patients (16.4%) showed additional cytogeneticaberrations. Nine patients (6.7%) harbored a variant Philadelphia chromosome,and most were in the chronic phase. Seventeen patients (12.7%) had major and minorroute abnormalities. There was a significant relationship between additional cytogeneticaberrations and major molecular response (P=0.032). Patient survival in the group withadditional cytogenetic aberrations was significantly lower (49.7±11.1 mo) than that inthe group without additional cytogenetic aberrations (77.3±3.1 mo) (P=0.031). ConclusionThis study revealed the same frequency of additional cytogenetic aberrations in CML asfound in previous studies. Additional chromosomal aberrations led to shorter survivaland lower rates of achievement of a major molecular response.

The effect of ginger and metoclopramide in the prevention of nausea and vomiting during and after surgery in cesarean section under spinal anesthesia

( Hamideh Pakniat ),( Fatemeh Lalooha ),( Farideh Movahed ),( Aynaz Boostan ),( Marzieh Beigom Khezri ),( Caleb Hedberg ),( Mohammad Ali Hosseini ) 대한산부인과학회 2020 Obstetrics & Gynecology Science Vol.63 No.2

Objective Postoperative nausea and vomiting is one of the most common side effects after anesthesia in surgeries, such as cesarean section. This study aimed to investigate the effect of ginger and metoclopramide in the prevention of nausea and vomiting during and after cesarean section. Methods This clinical trial was conducted on 180 patients aged 18-40 years who underwent cesarean section under spinal anesthesia. The first group received 10 mg of metoclopramide via intravenous injection (metoclopramide group), and the second group received 1 g of oral ginger (ginger group) half an hour before spinal anesthesia. The frequency and severity of nausea and vomiting during surgery and at 2, 6, 12, and 24 hours postoperatively were compared in both groups. To analyze the results, the t-test, chi-square test, and Mann-Whitney test were used. Results There was no significant difference in the frequency of nausea and vomiting between the 2 groups during operation, 2 hours and 6 hours after surgery (P=0.182, 0.444 and 0.563 respectively). The severity of nausea and vomiting was also similar in the 2 groups (P=0.487 and 0.652 respectively); however, the metoclopramide group had a lower systolic blood pressure (P<0.001; df=2.176; f=18.66) and mean arterial pressure (P<0.001; df=2.176; f=6.36) than the ginger group. Conclusion The results revealed that ginger reduced nausea and vomiting to the same extent as metoclopramide in patients undergoing cesarean section. Trial Registration Iranian Center for Clinical Trials Identifier: IRCT201611028611N3