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      • KCI등재

        Micro-grinding temperature prediction considering the effects of crystallographic orientation and the strain induced by phase transformation

        Man Zhao,Xia Jinan,Steven Y. Liang 한국정밀공학회 2019 International Journal of Precision Engineering and Vol.20 No.11

        This paper proposes a physical-based model to predict the temperature in the micro-grinding of maraging steel 3J33b with the consideration of material microstructure and process parameters. In micro-grinding, the effects of crystallography on the grinding machinability become significant, since the depth of cut is of the same order as the grain size. In this research, the Taylor factor model for multi-phase materials is proposed to quantify the crystallographic orientation (CO) with respect to the cutting direction by examining the number and type of activated slip systems. Then, the flow stress model is developed, in which both the athermal stress resulted from the COs and the strain induced by the phase transformation are taken into account. On the basis of the flow stress model, the grinding forces are predicted followed by the calculation of the grinding heat. In the investigation, the triangular heat flux distribution and the reported energy partition model are applied in the calculation of workpiece temperature. Furthermore, the temperature model is validated by conducting an orthogonal-designed experiment, with the predictions of the maximum temperature in good agreement with the experimental data. Moreover, the predictive data is compared with the predictions resulted from the two other previously reported models. The results indicate that the proposed temperature model with considering the effect of CO and the phase transformation improved the prediction accuracy of the micro-grinding temperature.

      • KCI등재

        Preparation, characterization and application of poly(sodium p-styrenesulfonate)/poly(methyl methacrylate) particles

        Man Zhang,Rui Wang,Tao Xiang,Wei-Feng Zhao,Chang-Sheng Zhao 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.34 No.-

        In this study, poly(sodium p-styrenesulfonate)/poly(methyl methacrylate) particles were facilelyprepared by in situ cross-linked polymerization followed with a liquid–liquid phase inversion technique. The particles exhibited selective adsorption for cationic dyes due to the negatively charged sulfonicgroups. The intra-particle diffusion process was the rate-limiting step for the adsorption of methyleneblue. More than 90% of cationic dye was removed by the adsorption column of the particles after threecirculations. The particles could be facilely fabricated and industrially used for wastewater treatment.

      • KCI등재

        Transcriptional profiling analysis of OsDT11-mediated ABA-dependent signal pathway for drought tolerance in rice

        Zhao Man,Ju Yanhu,Zhao Bo,Li Xiaoming,Dai LIli,Qu Jicheng,Chu Zhaohui,Ding Xinhua 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.5

        Small cysteine-rich peptides (CRPs) are important in plant responses to biotic and abiotic stresses. OsDT11, encoding an 88 amino acid CRP-type protein, has been reported to enhance tolerance to drought in rice (Oryza sativa L.) without negatively afecting other agronomic traits. However, the molecular mechanisms of OsDT11-mediated drought tolerance are still unclear. Here, we performed RNA-Seq analysis to compare the transcriptome profles between wild-type (WT) and OsDT11-overexpressing (OE) rice lines under drought stress or under control (non-drought) conditions. A total of 1570 and 1421 diferentially expressed genes were identifed in the OE lines and the WT under drought treatment, respectively, compared to non-drought conditions. Gene ontology analysis indicated that the 430 up-regulated genes in common to both OE and the WT lines were induced for functions related to responses to water deprivation and to abscisic acid (ABA). More than half of these genes had higher expression in the OE than in the WT under drought stress. In the OE, but not in the WT, 294 genes were specifcally up-regulated under drought stress and were functionally enriched in starch and sucrose biosyn�thetic processes and in response to stress. This implies that OsDT11 not only triggers strongly response to drought stress, but also alters several metabolic processes to enhance drought tolerance. Gene expression profling suggests that OsDT11 confers drought tolerance by mediating an enhanced response to drought stress in an ABA-dependent signaling pathways.

      • KCI등재

        Dietary methionine supplementation to a low-protein diet improved hair follicle development of Angora rabbits

        Zhao Man,Wang Tongtong,Wang Bin,Liu Chuanhua,Li Fuchang,Liu Lei 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.6

        Objective: Angora rabbits fed a low-protein diet exhibit decreased hair production performance. This study was set out to evaluate the effects of methionine on hair properties and nitrogen metabolism in Angora rabbits fed a low-protein diet and to investigate the gene expression related to hair follicle development to determine the possible molecular mechanism of methionine effects on hair follicle development. Methods: An experiment was conducted to investigate the effects of DL-methionine addition on a low-protein diet on hair development in Angora rabbits. Angora rabbits were divided into 5 groups: fed a normal diet (control), fed a low-protein diet (LP), or fed an LP supplemented with 0.2%, 0.4%, or 0.6% DL-methionine (Met). Results: The results showed that rabbits in the LP group had lower wool yield than the control rabbits, but the addition of 0.4% to 0.6% Met to LP attenuated these effects (p< 0.05). Dietary addition of 0.4% to 0.6% Met to LP increased the apparent nitrogen digestibility, nitrogen utilization rate, and feed efficiency (p<0.05). Feeding LP decreased the insulinlike growth factor 1 (IGF1), keratin-associated protein (KAP) 3.1, and KAP 6.1 mRNA levels compared with the control, but the addition of 0.4% Met in LP attenuated these effects (p<0.05). Relative to the LP or control group, dietary addition of 0.4% Met increased versican mRNA levels. Conclusion: In conclusion, the addition of Met to LP could improves wool production performance and feed efficiency and reduce nitrogen emissions in Angora rabbits. Met can promote hair follicle development, which may be associated with IGF1, KAP, and the versican signaling.

      • Using three-dimensional theory of elasticity for vibration analysis of laminated sectorial plates

        Liyuan Zhao,Man Wang,Rui Yang,Meng Zhao,Zenghao Song,N. Bohlooli 국제구조공학회 2023 Steel and Composite Structures, An International J Vol.48 No.1

        The main goal of this paper is to study vibration of damaged core laminated sectorial plates with Functionally graded (FG) face sheets based on three-dimensional theory of elasticity. The structures are made of a damaged isotropic core and two external face sheets. These skins are strengthened at the nanoscale level by randomly oriented Carbon nanotubes (CNTs) and are reinforced at the microscale stage by oriented straight fibers. These reinforcing phases are included in a polymer matrix and a three-phase approach based on the Eshelby-Mori-Tanaka scheme and on the Halpin-Tsai approach, which is developed to compute the overall mechanical properties of the composite material. Three complicated equations of motion for the sectorial plates under consideration are semi-analytically solved by using 2-D differential quadrature method. Using the 2-D differential quadrature method in the r- and z-directions, allows one to deal with sandwich annular sector plate with arbitrary thickness distribution of material properties and also to implement the effects of different boundary conditions of the structure efficiently and in an exact manner. The fast rate of convergence and accuracy of the method are investigated through the different solved examples. The sandwich annular sector plate is assumed to be simply supported in the radial edges while any arbitrary boundary conditions are applied to the other two circular edges including simply supported, clamped and free. Several parametric analyses are carried out to investigate the mechanical behavior of these multi-layered structures depending on the damage features, through-the-thickness distribution and boundary conditions.

      • KCI등재
      • Application of Slow-Freezing Cryopreservation Method for the Conservation of Diverse Potato (Solanum tuberosum L.) Genotypes

        Zhao Mei-Ai,Dhital Shambhu P.,Fang Yi-Lan,Khu Dong-Man,Song Ye-Su,Park Eung-Jun,Kang Chang-Won,Lim Hak-Tae The Korean Society of Plant Biotechnology 2005 Plant molecular biology and biotechnology research Vol.7 No.3

        Cryopreservation has been recognized as a practical and efficient tool for the long-term storage of vegetatively propagated plants. This study was conducted to investigate the effects of slow-freezing techniques on the cryopreservation of potato. In vitro plantlets of the potato genotypes of 'Atlantic', 'Superior’, 'Namseo', 'J138', and 'CTO5-5' were cold acclimated, and the excised axillary buds were precultured, osmoprotected, exposed to plant vitrification solution, frozen slowly to $-40^{\circ}C$ and then rapidly plunged into liquid nitrogen, thawed and finally plated on the regeneration medium. It was found that the higher the sucrose concentrations in the subculture medium of donor plantlets, the higher the survival rates of shoot tips after cryopreservation, and the highest survival (20%) was observed in the medium added with 0.25 M sucrose. As for the effect of cooling, $0.3^{\circ}C/min$ cooling speed showed the highest survival (25%). Different varieties showed different responses over different cryopreservation treatments. Survival rate was increased by slow-freezing technique method as compared with that of the basic cryopreservation method of vitrification alone in the diverse potato genotypes. Leaf and tuber morphologies of potatoes regenerated after cryopreservation using slow freezing technique were similar to those derived from the in vitro stock plantlets.

      • KCI등재

        Comparison and Analysis of Main Effects, Epistatic Effects, and QTL ${\times}$ Environment Interactions of QTLs for Agronomic Traits Using DH and RILs Populations in Rice

        Zhao, Xinhua,Qin, Yang,Jia, Baoyan,Kim, Suk-Man,Lee, Hyun-Suk,Eun, Moo-Young,Kim, Kyung-Min,Sohn, Jae-Keun 한국작물학회 2010 Journal of crop science and biotechnology Vol.13 No.4

        Two genetic linkage maps based on doubled haploid (DH) and recombinant inbred lines (RILs) populations, derived from the same indica-japonica cross 'Samgang x Nagdong', were constructed to analyze the quantitative trait loci (QTLs) affecting agronomic traits in rice. The segregations of agronomic traits in RILs population showed larger variations than those in DH population. A total of 10 and 12 QTLs were identified on six chromosomes using DH population and seven chromosomes using RILs population, respectively. Three stable QTLs including pl9.1, ph1.1, and gwp11.1 were detected through different years. The percentages of phenotypic variation explained by individual QTLs ranged from 8 to 18% in the DH population and 9 to 33% in the RILs population. Twenty-three epistatic QTLs were identified in the DH population, while 21 epistatic QTLs were detected in the RILs population. Epistatic interactions played an important role in controlling the agronomic traits genetically. Four significant main-effect QTLs were involved in the digenic interactions. Significant interactions between QTLs and environments (QE) were identified in two populations. The QTLs affecting grain weight per panicle (GWP) were more sensitive to the environmental changes. The comparison and QTLs analysis between two populations across different years should help rice breeders to comprehend the genetic mechanisms of quantitative traits and improve breeding programs in marker-assisted selection (MAS).

      • Small RNAs control sodium channel expression, nociceptor excitability, and pain thresholds.

        Zhao, Jing,Lee, Man-Cheung,Momin, Ali,Cendan, Cruz-Miguel,Shepherd, Samuel T,Baker, Mark D,Asante, Curtis,Bee, Lucy,Bethry, Audrey,Perkins, James R,Nassar, Mohammed A,Abrahamsen, Bjarke,Dickenson, Ant The Society 2010 The Journal of neuroscience Vol.30 No.32

        <P>To examine the role of small RNAs in peripheral pain pathways, we deleted the enzyme Dicer in mouse postmitotic damage-sensing neurons. We used a Nav1.8-Cre mouse to target those nociceptors important for inflammatory pain. The conditional null mice were healthy with a normal number of sensory neurons and normal acute pain thresholds. Behavioral studies showed that inflammatory pain was attenuated or abolished. Inflammatory mediators failed to enhance excitability of Nav1.8+ sensory neurons from null mutant mice. Acute noxious input into the dorsal horn of the spinal cord was apparently normal, but the increased input associated with inflammatory pain measured using c-Fos staining was diminished. Microarray and quantitative real-time reverse-transcription PCR (qRT-PCR) analysis showed that Dicer deletion lead to the upregulation of many broadly expressed mRNA transcripts in dorsal root ganglia. By contrast, nociceptor-associated mRNA transcripts (e.g., Nav1.8, P2xr3, and Runx-1) were downregulated, resulting in lower levels of protein and functional expression. qRT-PCR analysis also showed lowered levels of expression of nociceptor-specific pre-mRNA transcripts. MicroRNA microarray and deep sequencing identified known and novel nociceptor microRNAs in mouse Nav1.8+ sensory neurons that may regulate nociceptor gene expression.</P>

      • KCI등재

        Effects of Alkyl Chain Length of Aliphatic Dicarboxylic Ester on Degradation Properties of Aliphatic-Aromatic Water-Soluble Copolyesters for Warp Sizing

        Manli Li,Enqi Jin,Zhiyong Qiao,Rongli Zhao 한국섬유공학회 2018 Fibers and polymers Vol.19 No.3

        In order to study the effects of alkyl chain length of aliphatic dicarboxylic ester (ADE) monomers on enzymatic and hydrolytic degradation properties of aliphatic-aromatic water-soluble copolyesters for warp sizing, dimethyl terephthalate, dimethyl isophthalate-5-sulfonic sodium, and ADE monomers with various alkyl chain lengths were copolymerized through a two-step method, i.e. transesterification and polycondensation. The enzymatic and hydrolytic degradation properties of the copolyesters were studied in terms of reduction rates of molecular weight, glass transition temperatures, and surface morphology after being cultivated for 24-96 h. It was found that, enzymatic degradation of the copolyesters strongly depended on alkyl chain length of ADE monomers. After being enzymatically degraded for 96 h, reduction rate of molecular weight of the copolyester using dimethyl malonate as ADE monomers could reach 22.2 %. Meanwhile, the hydrolytic degradation of the copolyesters was not directly related to the alkyl chain length.

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