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이병준 ( Byungjoon Lee ),박진선 ( Jinseon Park ),이상익 ( Sangik Lee ),이종혁 ( Jonghyuk Lee ),최원 ( Won Choi ),이준구 ( Joongu Lee ),윤성수 ( Seongsoo Yoon ) 한국농공학회 2018 한국농공학회 학술대회초록집 Vol.2018 No.-
농업생산기반시설물 중 양수장은 전국 7,052개로 그 중 3479개는 20년 이상 운영되고 있어 노후화 현상에 대한 우려가 있다. 노후화된 양수장을 재구축 및 신축하기에는 과다한 비용이 소요된다. 따라서 기존 시설의 성능을 정량적으로 판단하여 유지관리 할 필요성이 있다. 농업생산기반 정비사업계획 설계기준에 의하면 양수장의 유지관리는 펌프의 운전목적을 달성할 수 있도록 각 설비 기능들을 정상으로 유지하는 것을 목적으로 한다. 또한 양수장의 성능을 평가하기 위한 항목으로 토목, 전기, 기계, 건축으로 분류하고 있다. 구조물은 양수장의 펌프를 외부환경으로부터 보호해야하기 때문에 노후화에 대한 판단이 필요하지만 펌프관리에 비해 상대적으로 구조물에 대한 운영관리 체계는 구체적이지 못한 실정이다. 이를 보완하기 위해 VE(Value Engineering)를 도입하고자 한다. VE는 가치를 얻기 위한 목적으로 수행되는 프로젝트의 기능분석을 통해 대안창출을 할 수 있는 체계적인 프로세스를 의미한다. 이를 통해 양수장 유지관리 항목에 대해 기능은 유지하면서 역할을 세분화하고 중요도를 설정해 성능을 정량적 평가 할 수 있다. 따라서 본 연구에서는 VE를 통해 양수장에 대한 기능과 성능을 정량적으로 평가하고 기존의 유지관리 방안에 대한 개선사항을 제시하고자 한다.
Eui-Seon Lee,Tae-Young Kim,Yam Prasad Aryal,Kihyun Kim,Seongsoo Byun,Dongju Song,Yejin Shin,Dany Lee,Jooheon Lee,Gilyoung Jung,Seunghoon Chi,Yoolim Choi,Youngkyun Lee,Chang-Hyeon An,Jae-Young Kim 대한구강생물학회 2021 International Journal of Oral Biology Vol.46 No.2
This study summarizes the recent cutting-edge approaches for dentin regeneration that still do not offer adequate solutions. Tertiary dentin is formed when odontoblasts are directly affected by various stimuli. Recent preclinical studies have reported that stimulation of the Wnt/β-catenin signaling pathway could facilitate the formation of reparative dentin and thereby aid in the structural and functional development of the tertiary dentin. A range of signaling pathways, including the Wnt/β-catenin pathway, is activated when dental tissues are damaged and the pulp is exposed. The application of small molecules for dentin regeneration has been suggested as a drug repositioning approach. This study reviews the role of Wnt signaling in tooth formation, particularly dentin formation and dentin regeneration. In addition, the application of the drug repositioning strategy to facilitate the development of new drugs for dentin regeneration has been discussed in this study.
Nahm, Minyeop,Kim, Sungdae,Paik, Sang Kyoo,Lee, Mihye,Lee, Seongsoo,Lee, Zang Hee,Kim, Jaesang,Lee, Daekee,Bae, Yong Chul,Lee, Seungbok The Society 2010 The Journal of neuroscience Vol.30 No.24
<P>The bone morphogenetic protein (BMP) ligand Glass bottom boat (Gbb) acts as a retrograde growth signal at the Drosophila neuromuscular junction (NMJ). Endocytic regulation of presynaptic BMP receptors has been proposed to attenuate retrograde BMP signaling. However, it remains unknown whether the Gbb signal is also regulated by postsynaptic mechanisms. Here, we provide evidence that Drosophila Cdc42-interacting protein 4 (dCIP4) functions postsynaptically to inhibit synaptic growth. dCIP4 is localized postsynaptically at NMJs. dcip4 mutations lead to synaptic overgrowth and increased presynaptic phosphorylated mothers against decapentaplegic (Mad) levels, and these defects are rescued by muscle-specific expression of dCIP4. Biochemical and genetic analyses demonstrate that dCIP4 acts downstream of Cdc42 to activate the postsynaptic Wsp-Arp2/3 pathway. We also show that BMP signaling is necessary for synaptic overgrowth in larvae lacking postsynaptic dcip4 or wsp. Finally, dCIP4 and Wsp inhibit Gbb secretion. Thus, we propose that dCIP4 restrains synaptic growth by inhibiting postsynaptic Gbb secretion through the Wsp-Arp2/3 pathway.</P>
Production and Breeding of Transgenic Cloned Pigs Expressing Human CD73
Lee, Seung-Chan,Lee, Haesun,Oh, Keon Bong,Hwang, In-Sul,Yang, Hyeon,Park, Mi-Ryung,Ock, Sun-A,Woo, Jae-Seok,Im, Gi-Sun,Hwang, Seongsoo The Korean Society of Developmental Biology 2017 발생과 생식 Vol.21 No.2
One of the reasons to causing blood coagulation in the tissue of xenografted organs was known to incompatibility of the blood coagulation and anti-coagulation regulatory system between TG pigs and primates. Thus, overexpression of human CD73 (hCD73) in the pig endothelial cells is considered as a method to reduce coagulopathy after pig-to-non-human-primate xenotransplantation. This study was performed to produce and breed transgenic pigs expressing hCD73 for the studies immune rejection responses and could provide a successful application of xenotransplantation. The transgenic cells were constructed an hCD73 expression vector under control porcine Icam2 promoter (pIcam2-hCD73) and established donor cell lines expressing hCD73. The numbers of transferred reconstructed embryos were $127{\pm}18.9$. The pregnancy and delivery rate of surrogates were 8/18 (44%) and 3/18 (16%). The total number of delivered cloned pigs were 10 (2 alive, 7 mummy, and 1 died after birth). Among them, three live hCD73-pigs were successfully delivered by Caesarean section, but one was dead after birth. The two hCD73 TG cloned pigs had normal reproductive ability. They mated with wild type (WT) MGH (Massachusetts General Hospital) female sows and produced totally 16 piglets. Among them, 5 piglets were identified as hCD73 TG pigs. In conclusion, we successfully generated the hCD73 transgenic cloned pigs and produced their litters by natural mating. It can be possible to use a mate for the production of multiple transgenic pigs such as ${\alpha}-1,3-galactosyltransferase$ knock-out /hCD46 for xenotransplantation.
Seongsoo Hwang,In-Sul Hwang,Mi Ryung Park,Jeong Hee Yoon,Jeong-Woong Lee,Kichoon Lee,Gi Sun Im 한국동물생명공학회(구 한국동물번식학회) 2018 발생공학 국제심포지엄 및 학술대회 Vol.2018 No.06
Understanding the sophisticated imprinting pattern is the first challenge for deciphering the molecular mechanism of an imprinted gene. However, the determination of imprinted genes, and their methylation status in pigs, remains inefficient at present. Herein, for the first time, we have made an attempt to illustrate the complex imprinting pattern of porcine GNAS complex locus by an efficient strategy using RNA-seq analysis of parthenogenetic fetuses containing no paternal allele, but two maternal alleles. RNA-seq results showed that Nespas, Gnasxl, and Exon 1A genes expressed at extremely lower levels (p<0.05) in parthenogenetic fetuses compared to the normally fertilized fetuses, suggest these three genes are paternally-expressed. Whereas, the expression levels of Gnas were similar in both groups, suggesting biallelic expression of Gnas. The parental-specific expression of these genes was further confirmed by quantitative real-time PCR. Bisulfite sequencing revealed that the maternally derived Nespas promoter was methylated, but the paternal chromosome was not methylated. Our finding provided full-scale imprinting information on porcine GNAS locus for the first time. Furthermore, our study is useful for identify novel imprinted genes, confirm the previously known imprinted genes, and determine imprinting pattern efficiently.
Lee, Seongsoo,Park, Junyong,Cho, Kyungjun,Lee, Hyunsuk,Seo, Chulhun,Ahn, Seungyoung,Kim, Joungho,Kim, Dong-Hyun,Cho, Yeonje,Kim, Hongseok,Song, Chiuk,Jeong, Seungtaek,Song, Jinwook,Park, Gyeyoung,Hong Institute of Electrical and Electronics Engineers 2019 IEEE transactions on industrial electronics Vol.66 No.6
<P>In this paper, we first proposed a novel hybrid loop array (HLA) for low leakage electromagnetic field (EMF) level and high efficiency in a wireless high power transfer system. The proposed HLA effectively enhances the system efficiency and shields leakage EMF in a wireless power transfer (WPT) system using kHz range resonant frequency. The key originality of the proposed HLA is the combination of two types of loop coil; shielding loop coil (SLC) and amplifying loop coil (ALC). SLCs reduce leakage EMF, and ALCs significantly enhance the magnetic field from a Tx coil. The simulation and experiment results show that the proposed solution successfully overcomes the limitations of the existing solutions. Analytical modeling and design procedure are introduced and discussed. In addition, the experimental verification of the simulation result is included. We first designed and modeled an HLA considering the coupling effect of neighboring loop coils to evaluate its efficiency and leakage EMF. With the proposed HLA, we demonstrated a 9.36% improvement in the efficiency and 3 dBm reduction in the leakage EMF near the WPT system.</P>
Lee, Kyu-Sun,Huh, Sungun,Lee, Seongsoo,Wu, Zhihao,Kim, Ae-Kyeong,Kang, Ha-Young,Lu, Bingwei National Academy of Sciences 2018 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.115 No.38
<P><B>Significance</B></P><P>Ca<SUP>2</SUP><SUP>+</SUP> regulates cellular metabolism, proliferation, and differentiation. Ca<SUP>2+</SUP> homeostasis is critical for cellular function and health. Mitochondria help buffer transient Ca<SUP>2+</SUP> elevations and prevent cell death induced by Ca<SUP>2+</SUP> overload. Mito-Ca<SUP>2+</SUP> is also required for optimal activity of certain key mitochondrial functions, such as oxidative phosphorylation and metabolism. Thus, mito-Ca<SUP>2+</SUP> homeostasis assumes central roles in cellular health. Endoplasmic reticulum (ER) and mitochondria make intimate contacts and exchange molecules such as Ca<SUP>2+</SUP> and lipids. We find that ER-to-mitochondria Ca<SUP>2+</SUP> transfer is important for mito-Ca<SUP>2+</SUP> homeostasis and that the conserved Miro protein is critically involved. We show that mito-Ca<SUP>2+</SUP> homeostasis is disrupted in neurodegenerative disease models and its restoration is beneficial. Our findings have important implications for therapeutic intervention of neurodegenerative diseases.</P><P>Calcium (Ca<SUP>2+</SUP>) homeostasis is essential for neuronal function and survival. Altered Ca<SUP>2+</SUP> homeostasis has been consistently observed in neurological diseases. How Ca<SUP>2+</SUP> homeostasis is achieved in various cellular compartments of disease-relevant cell types is not well understood. Here we show in <I>Drosophila</I> Parkinson’s disease (PD) models that Ca<SUP>2+</SUP> transport from the endoplasmic reticulum (ER) to mitochondria through the ER–mitochondria contact site (ERMCS) critically regulates mitochondrial Ca<SUP>2+</SUP> (mito-Ca<SUP>2+</SUP>) homeostasis in dopaminergic (DA) neurons, and that the PD-associated PINK1 protein modulates this process. In <I>PINK1</I> mutant DA neurons, the ERMCS is strengthened and mito-Ca<SUP>2+</SUP> level is elevated, resulting in mitochondrial enlargement and neuronal death. Miro, a well-characterized component of the mitochondrial trafficking machinery, mediates the effects of PINK1 on mito-Ca<SUP>2+</SUP> and mitochondrial morphology, apparently in a transport-independent manner. Miro overexpression mimics <I>PINK1</I> loss-of-function effect, whereas inhibition of Miro or components of the ERMCS, or pharmacological modulation of ERMCS function, rescued <I>PINK1</I> mutant phenotypes. Mito-Ca<SUP>2+</SUP> homeostasis is also altered in the LRRK2-G2019S model of PD and the PAR-1/MARK model of neurodegeneration, and genetic or pharmacological restoration of mito-Ca<SUP>2+</SUP> level is beneficial in these models. Our results highlight the importance of mito-Ca<SUP>2+</SUP> homeostasis maintained by Miro and the ERMCS to mitochondrial physiology and neuronal integrity. Targeting this mito-Ca<SUP>2+</SUP> homeostasis pathway holds promise for a therapeutic strategy for neurodegenerative diseases.</P>
The Evolution of Korea's Basic Acts on Science and Technology and their Characteristics
Lee, Changyul,Lee, Elly Hyanghee,Kim, Seongsoo Asian Society for Innovation and Policy 2021 Asian Journal of Innovation and Policy Vol.10 No.3
This study examined the evolution of S&T Basic Acts in Korea from the Science and Technology Promotion Act (1967) through the Special Act on STI (1997) to the Framework Act on Science and Technology (2001) in the following aspects: 1) comprehensive plans, 2) coordination mechanisms for S&T policies, 3) enforcement of R&D programs and performance diffusion, 4) promotion of human resources, 5) and S&T investment and budgeting. Before the Framework Act on S&T was enacted in 2001, critical issues were found in establishing S&T master plans, promotion of R&D programs, comprehensive coordination mechanisms, and R&D budgeting. The three Basic Acts have expanded the scope of regulation over time to cover the entire cycle of the S&T process. They concern a wide range of issues, including creating a basis for scientific and technological development, S&T promotion, disseminating and commercializing research outcomes, and preventing adverse effects from science and technology. The content of the Basic Acts has evolved in response to changes in the political, economic, and social environment of Korean industry during the past five decades.