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Kwon Nayeon,Lee Siyun,Jang Moonbong,Lee Jin-Ho,Park Chulhwan,Lee Taek 한국바이오칩학회 2024 BioChip Journal Vol.18 No.1
Yellow fever virus (YFV) is an acute infectious virus with high morbidity and mortality risks during the toxic phase. Early diagnosis and suppression are essential because YFV has no precise treatment. With the aim of detecting YFV, we fabricated a highly sensitive electrochemical biosensor comprised with a truncated DNA aptamer/MXene heterolayer. The synthesized DNA aptamer was prepared by systematic evolution of ligands using the exponential enrichment (SELEX) technique, which can specifically detect the YFV NS1 protein. MXenes increase the electrical sensitivity and the possibility of attachment of aptamers by widening the surface area. The aptamer-cutting process which called a truncation process can reduce the production cost of biosensors. The biosensor performance was evaluated using electrochemical methods, cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The limit of detection (LOD) was 2.757 pM for YFV diluted in phosphate-buffered saline (PBS) and 2.366 pM for YFV diluted in 10% human serum, proving that the biosensor specifically binds to YFV through selectivity evaluation. This biosensor can be a valuable tool for the early diagnosis of YFV, enabling timely intervention as well as facilitating the control and prevention of yellow fever outbreaks.
Kwon Hyo Eun,Ko Nayeon,Yuk Doyoung,Choi Seo Won,Koh Seong-Eun 대한재활의학회 2023 Annals of Rehabilitation Medicine Vol.47 No.5
Objective: To systematically review the effects of protein supplementation in older adults with sarcopenia.Methods: A systematic literature search was conducted in PubMed, Cochrane Library, and Embase databases until May 2023. The inclusion criteria were as follows: (1) randomized controlled trials with a quantitative study design; (2) studies with a study group of older adults with sarcopenia; (3) studies comparing muscle mass, muscle strength, and performance of older adults with sarcopenia after protein supplementation; and (4) studies published up to May 2023.Results: Six retrospective comparative studies, including 715 patients, met the inclusion criteria. The nutritional supplementation group exhibited significant improvement in appendicular skeletal muscle mass (standardized mean difference [SMD]=0.41; 95% confidence interval [CI], 0.24–0.58; p<0.001; I<sup>2</sup>=1%), while handgrip strength (SMD=0.37; 95% CI, -0.32–1.07; p=0.29; I<sup>2</sup>=94%) and Short Physical Performance Battery (SPPB) (SMD=0.35; 95% CI, -0.47–1.18; p=0.40; I<sup>2</sup>=94%) showed a tendency for improvement.Conclusion: Nutritional supplementation with protein increased appendicular muscle mass in older adults with sarcopenia and improved handgrip strength and SPPB scores.
Ginsenoside Rk1 is a novel inhibitor of NMDA receptors in cultured rat hippocampal neurons 490
Nayeon Ryoo,Md. Ataur Rahman,Hongik Hwang,Sung Kwon Ko,Seung-Yeol Nah,Hyoung-Chun Kim,Hyewhon Rhim 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.3
Background: Ginsenoside Rk1, a saponin component isolated from heat-processed Panax ginseng Meyer,has been implicated in the regulation of antitumor and anti-inflammatory activities. Although ourprevious studies have demonstrated that ginsenoside Rg3 significantly attenuated the activation ofNMDA receptors (NMDARs) in hippocampal neurons, the effects of ginsenosides Rg5 and Rk1, which arederived from heat-mediated dehydration of ginsenoside Rg3, on neuronal NMDARs have not yet beenelucidated. Methods: We examined the regulation of NMDARs by ginsenosides Rg5 and Rk1 in cultured rat hippocampalneurons using fura-2ebased calcium imaging and whole-cell patch-clamp recordings. Results: The results from our investigation showed that ginsenosides Rg3 and Rg5 inhibited NMDARswith similar potencies. However, ginsenoside Rk1 inhibited NMDARs most effectively among the fivecompounds (Rg3, Rg5, Rk1, Rg5/Rk1 mixture, and protopanaxadiol) tested in cultured hippocampalneurons. Its inhibition is independent of the NMDA- and glycine-binding sites, and its action seems toinvolve in an interaction with the polyamine-binding site of the NMDAR channel complex. Conclusion: Taken together, our results suggest that ginsenoside Rk1 might be a novel componentcontributable to the development of ginseng-based therapeutic treatments for neurodegenerativediseases.
Ginsenoside Rk1 is a novel inhibitor of NMDA receptors in cultured rat hippocampal neurons
Ryoo, Nayeon,Rahman, Md. Ataur,Hwang, Hongik,Ko, Sung Kwon,Nah, Seung-Yeol,Kim, Hyoung-Chun,Rhim, Hyewhon The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.3
Background: Ginsenoside Rk1, a saponin component isolated from heat-processed Panax ginseng Meyer, has been implicated in the regulation of antitumor and anti-inflammatory activities. Although our previous studies have demonstrated that ginsenoside Rg3 significantly attenuated the activation of NMDA receptors (NMDARs) in hippocampal neurons, the effects of ginsenosides Rg5 and Rk1, which are derived from heat-mediated dehydration of ginsenoside Rg3, on neuronal NMDARs have not yet been elucidated. Methods: We examined the regulation of NMDARs by ginsenosides Rg5 and Rk1 in cultured rat hippocampal neurons using fura-2-based calcium imaging and whole-cell patch-clamp recordings. Results: The results from our investigation showed that ginsenosides Rg3 and Rg5 inhibited NMDARs with similar potencies. However, ginsenoside Rk1 inhibited NMDARs most effectively among the five compounds (Rg3, Rg5, Rk1, Rg5/Rk1 mixture, and protopanaxadiol) tested in cultured hippocampal neurons. Its inhibition is independent of the NMDA- and glycine-binding sites, and its action seems to involve in an interaction with the polyamine-binding site of the NMDAR channel complex. Conclusion: Taken together, our results suggest that ginsenoside Rk1 might be a novel component contributable to the development of ginseng-based therapeutic treatments for neurodegenerative diseases.
( Inbeom Kwon ),( Nayeon Choi ),( Ji Hui Shin ),( Seunghun Lee ),( Bora Nam ),( Tae-hwan Kim ) 대한류마티스학회 2024 대한류마티스학회지 Vol.31 No.1
Objective: To investigate the effects of anti-tumor necrosis factor (TNF) treatment on lipid profiles and identify risk factors for an increase in total cholesterol (TC) after the anti-TNF treatment in ankylosing spondylitis (AS) patients. Methods: This retrospective cohort study analyzed AS patients who received the first-line anti-TNF treatment. Patients with at least nine months of follow-up were included; those who were under 18 years or on any lipid-lowering agent were excluded. A linear mixed model was used to assess the impact of anti-TNF inhibitors on disease activity and lipid profile (TC, low-density lipoprotein [LDL], high-density lipoprotein [HDL], and triglycerides [TG]). Univariable and multivariable linear regression were used to identify risk factors for an increase in TC after 3 months of anti-TNF treatment. Results: A total of 315 AS patients were enrolled (78.1% male, median age 32.0 [26.0~41.0]). TC, HDL, and TG levels significantly increased particularly within the first 3 months of anti-TNF treatment, while LDL level did not show significant changes. Changes in inflammatory markers and lipid particles (TC, LDL, TG) were correlated over time, but HDL showed no significant correlation. Older age, higher baseline erythrocyte sedimentation rate, and lower baseline LDL level were related to an increase in TC after 3 months of the anti-TNF treatment. Conclusion: In AS patients, anti-TNF treatment has been found to increase lipid particles, potentially due to its anti-inflammatory effects. Future research should explore the underlying mechanism and the clinical implications of dyslipidemia, particularly the occurrence of cardiovascular events, following anti-TNF treatment in AS patients.
Lee, Nayeon,Park, Jae Woo,Kim, Hyung Joon,Yeon, Ju Hun,Kwon, Jihye,Ko, Jung Jae,Oh, Seung-Hun,Kim, Hyun Sook,Kim, Aeri,Han, Baek Soo,Lee, Sang Chul,Jeon, Noo Li,Song, Jihwan Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.6
Microfluidics can provide unique experimental tools to visualize the development of neural structures within a microscale device, which is followed by guidance of neurite growth in the axonal isolation compartment. We utilized microfluidics technology to monitor the differentiation and migration of neural cells derived from human embryonic stem cells (hESCs). We co-cultured hESCs with PA6 stromal cells, and isolated neural rosette-like structures, which subsequently formed neurospheres in suspension culture. Tuj1-positive neural cells, but not nestin-positive neural precursor cells (NPCs), were able to enter the microfluidics grooves (microchannels), suggesting that neural cell-migratory capacity was dependent upon neuronal differentiation stage. We also showed that bundles of axons formed and extended into the microchannels. Taken together, these results demonstrated that microfluidics technology can provide useful tools to study neurite outgrowth and axon guidance of neural cells, which are derived from human embryonic stem cells.
( Mi-hyang Lee ),( Nayeon Kwon ),( So Ra Yoon ),( Oh Yoen Kim ) 한국임상영양학회 2016 Clinical Nutrition Research Vol.5 No.3
We hypothesized that lower proportion of serum phospholipid docosahexaenoic acid (DHA) is inversely associated with increased cardiovascular risk and vascular function in metabolically healthy men. To elucidate it, we first compared serum phospholipid free fatty acid (FA) compositions and cardiovascular risk parameters between healthy men (n = 499) and male patients with coronary artery disease (CAD, n = 111) (30-69 years) without metabolic syndrome, and then further-analyzed the association of serum phospholipid DHA composition with arterial stiffness expressed by brachial-ankle pulse wave velocity (ba- PWV) in metabolically healthy men. Basic parameters, lipid profiles, fasting glycemic status, adiponectin, high sensitivity C-reactive protein (hs-CRP) and LDL particle size, and serum phospholipid FA compositions were significantly different between the two subject groups. Serum phospholipid DHA was highly correlated with most of long-chain FAs. Metabolically healthy men were subdivided into tertile groups according to serum phospholipid DHA proportion: lower (< 2.061%), middle (2.061%-3.235%) and higher (> 3.235%). Fasting glucose, insulin resistance, hs-CRP and ba-PWVs were significantly higher and adiponectin and LDL particle size were significantly lower in the lower-DHA group than the higher- DHA group after adjusted for confounding factors. In metabolically healthy men, multiple stepwise regression analysis revealed that serum phospholipid DHA mainly contributed to arterial stiffness (β′-coefficients = -0.127, p = 0.006) together with age, systolic blood pressure, triglyceride (r = 0.548, p = 0.023). Lower proportion of serum phospholipid DHA was associated with increased cardiovascular risk and arterial stiffness in metabolically healthy men. It suggests that maintaining higher proportion of serum phospholipid DHA may be beneficial for reducing cardiovascular risk including arterial stiffness in metabolically healthy men.