Water-soluble Genistin Glycoside Isoflavones Upregulate Antioxidant Metallothionein Expression and Scavenge Free Radicals

Chung, Mi-Ja,Kang, Ah-Young,Jun, Hee-Jin,Kim Sang-Yeon,Moon, Tae-Hwa,Park, Kwan-Hwa,Lee, Sung-Joon 생명자원연구 2005 생명자원연구 Vol.13 No.-

Effects of dietary supplementation with probiotic CS-A on performance in broiler chickens

Seong Soo Kang1*, Se Eun Kim, Ara Go, Kyung Mi Shim, Chun Sik Bae, Chang Jong Moon, Sung-Ho Kim, Jong-Choon Kim, Jin-Cheol Yoo, Seung Sik Cho 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.3

Probiotics, enzymes, organic acids, oligosaccharides, antioxidants, and other functional materials are actively being explored as alternatives to antibiotics. Probiotics include live beneficial microorganisms that colonize the intestinal tract and competitively inhibit attachment and growth of harmful microbes. Probiotics also increase feed efficiency by assisting in nutrient absorption and digestion. The current study was conducted in order to evaluate the effect of a new probiotic, CS-A, as a dietary supplement of a fermented product on growth performance, feed intake, and feed conversion efficiency in broiler chickens, and to evaluate its value as an alternative for antibiotics used as a feed additive. Antibacterial and anti-inflammatory effects of CS-A were investigated in vitro and the in vivo effects of a constant concentration of supplemented CS-A on growth rate and feed efficiency were evaluated. In addition, the safety of CS-A was assessed by examination of common symptoms and mortality. Determination of minimal inhibitory concentration revealed an excellent antibacterial effect of CS-A. Cytotoxicity was low and anti-inflammatory effects were achieved at the effective concentration of CS-A. Supplementation with 0.1% CS-A resulted in a feed efficiency score of 1.84 in broilers, compared to 2.00 in the control group. There were no adverse clinical findings, necropsy findings, hematology, and altered serum biochemistry parameters, and no mortality. Thus, it is concluded that CS-A is safe and effective as a feed additive.

The Results of Curative Radiotherapy for Carcinoma of Uterine cervix

강기문,유미령,장지영,서태석,윤세철,박용휘,신경섭,남궁성은,김승조,Kang Ki Mun,Ryu Mi Ryeong,Chang Gee Young,Suh Tae Suk,Yoon Sei Chul,Bahk Yong Whee,Shinn Kyung Sub,Namkoong Sung Eun,Kim Seung Jo The Korean Society for Radiation Oncology 1993 Radiation Oncology Journal Vol.11 No.1

가톨릭의과대학 강남성모병원 치료방사선과에서 1983년 3월부터 1989년 10월까지 79개월 동안에 자궁경부암으로 근치적 방사선치료를 받았던 135명의 환자들 중에서 추적이 가능하였던 120명의 환자들을 대상으로 치료결과및 예후에 영향을 미치는 인자에 대하여 후향적 분석을 하였다. 방사선 단독으로 치료한 환자는 78명이었고 유도 화학요법을 방사선 치료전에 시행한 환자는 42명이었다. 대상 환자들의 추적 조사기간은,2개월에서 106개월이었고 중간 추적조사 기간은 62개월이었다. 환자들의 나이는 32세부터 79세까지의 분포를 보였다(중앙값, 59세). FIGO 병기별 분류에 의하면, IB 기가 20명 ($16.7{\%}$), IIA 기가 19명 ($15.8{\%}$), IIB기가 49명 ($40.8{\%}$), IIIA 기가 5명 ($4.2{\%}$), IIIB기가 13명 ($10.8{\%}$), IVA 기가 14명 ($11.7{\%}$)이었다. 전체환자의 5년 생존율은 $50.8{\%}$였다. 병기별 5년 생존율은 IB 기가 $47.7{\%}$ IIA 기가 $70.2{\%}$, IIB 기가 $64.1{\%}$, IIIA 기가 $40.0{\%}$, IIIB 기가 $23.1{\%}$, IVA 기가 $14.3{\%}$였다. 치료방법에 따른 5년 생존율은 방사선 단독으로 치료한 환자가 $51.2{\%}$였고, 유도화학요법을 방사선 치료전에 시행한 환자는 $54.0{\%}$였다. 치료후 재발은 22명 ($18.3{\%}$,)에서 관찰되었고, 이중 14명 ($11.7{\%}$)에서 국소재발이, 7명 ($5.8{\%}$)에서 원격전이가, 1명 ($0.8{\%}$)에서 국소재발과 원격전이가 함께 발생하였다. 그리고, 치료에 의한 합병증은 41명 ($34.2{\%}$)에서 관찰되었으며 9명 ($7.5{\%}$)에서 습낙설, 8명 ($7.5{\%}$)에서 설사, 7명 ($5.8{\%}$)에서 방사선 직장염의 순으로 발생하였다. 예후와 관련된 생존율에 영향을 주었던 인자로는 나이 (p<0.0291), 병기(p<0.0001), 전신상태(p<0.0041), 초기 혈색소 수치 (p<0.0001), 강내 조사(p<0.0004)였고, 조직학적 소견(p<0.29), 유도 화학요법과의 병행치료(p<0.87)는 통계학적으로 유의하지 않았다. This is a retrospective analysis of 135 patients with invasive carcinoma of the uterine cervix treated with curative radiotherapy from March 1983 through October 1989 at the Department of Therapeutic Radiology, Kang-Nam 51. Mary's Hospital. Among them, 78 patients received radiotherapy alone and 42 patients treated with neoadjuvant chemotherapy followed by radiotherapy and 15 patients were lost to follow up. All patients had follow up from 2 to 106 months (median; 62 months). Age of the patients ranged from 32 to 79 years at presentation (median; 59 years). According to FIGO classification, there were 20 ($16.7{\%}$) in stage IB, 19 ($15.8{\%}$) in stage IIA,49 ($40.8{\%}$) in stage IIB, 5 ($4.2{\%}$) in stage IIIA, 13 ($10.8{\%}$,) in stage IIIB,14 ($11.7{\%}$) in stage IVA. The pathological classification showed 96 ($80.0{\%}$) squamous cell carcinomas, 5 ($4.2{\%}$) adenocarcinomas and 19 ($15.8{\%}$) proven by cytology. The overall 5-year survival rates was $50.8{\%}$, and the 5-year survival rates by stage IB, IIA, IIB, IIIA, IIIB, IVA was $47.7{\%},\;70.2{\%},\;64.1{\%},\;40.0{\%},\;23.1{\%},\;14.3{\%}$, respectively. The 5-year survival rates was noted $51.2{\%}$ of radiotherapy alone and $50.4{\%}$of neoadjuvant chemotherapy followed by radiotherapy. The overall failure rate was $18.3{\%}$(22/120) including $11.7{\%}$ (14/120) locoregional failure, $5.8{\%}$ (7/120) distant metastasis and $0.8{\%}$(1/120) locoregional failure with distant metastasis. Treatment failure rates by the stages were $15{\%}$ (3/20) in stage IB. $10.5{\%}$ (2/19) in stage IIA, $10.2{\%}$, (5/49) in stage IIB, $20{\%}$ (1/5) in stage IIIA, $61.5{\%}$(8/13) in stage IIB, and $28.6{\%}$ (4/14) in stage IVA. The overall complication rate was $34.2{\%}$(41/120) including wet desquamation $7.5{\%}$, (9/120), diarrhea $6.7{\%}(8/120), radiation proctitis $5.8{\%}$(7/120) in decreasing order. A multivariate analysis of factors influencing the survival showed patient age (p < 0.0291), FIGO stage (p<0.0001), Karnofsky performance status (p<0.0043), initial hemoglobin level (p<0.0001), and intracavitary radiation (p<0.0004), but, no significancy in histology (p<0.29) and treatment method (p < 0.87).

Co-culture with NK-92MI cells enhanced the anti-cancer effect of bee venom on NSCLC cells by inactivation of NF-${\kappa}B$

Kollipara, Pushpa Saranya,Kim, Jung Hyun,Won, Dohee,Lee, Sang Min,Sung, Ha Chang,Chang, Hyun Sok,Lee, Kang Tae,Lee, Kang Sik,Park, Mi Hee,Song, Min Jong,Song, Ho Sueb,Hong, Jin Tae 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.3

In the present study we experimented on a multimodal therapeutic approach, such as combining chemotherapy agent (Bee venom) with cellular (NK-92MI) immunotherapy. Previously bee venom has been found to show anti-cancer effect in various cancer cell lines. In lung cancer cells bee venom showed an $IC^{50}$ value of $3{\mu}g/ml$ in both cell lines. The co-culture of NK-92MI cell lines with lung cancer cells also show a decrease in viability upto 50 % at 48 h time point. Hence we used bee venom treated NK-92MI cells to co-culture with NSCLC cells and found that there is a further decrease in cell viability upto 70 and 75 % in A549 and NCI-H460 cell lines respectively. We further investigated the expression of various apoptotic and anti-apoptotic proteins and found that Bax, cleaved caspase-3 and -8 were increasing where as Bcl-2 and cIAP-2 was decreasing. The expression of various death receptor proteins like DR3, DR6 and Fas was also increasing. Concomitantly the expression of various death receptor ligands (TNFalpha, Apo3L and FasL) was also increasing of NK-92MI cells after co-culture. Further the DNA binding activity and luciferase activity of NF-${\kappa}B$ was also inhibited after co-culture with bee venom treated NK-92MI cell lines. The knock down of death receptors with si-RNA has reversed the decrease in cell viability and NF-${\kappa}B$ activity after co-culture with bee venom treated NK-92MI cells. Thus this new approach can enhance the anti-cancer effect of bee venom at a much lower concentration.

Syntheses and Photovoltaic Properties of Solar Cells Based on Porphyrin Derivatives and MEH-PPV

( Sung-Hae Park ),( Mi-Ra Kim ),( Eun-Hee Kang ),( Jin-Kyung Ham ),( Jin-Kook Lee ) 한국공업화학회 2004 한국공업화학회 연구논문 초록집 Vol.2004 No.-

Predictive Modeling of Staphylococcus aureus Growth on Gwamegi (semidry Pacific saury) as a Function of Temperature

Kang, Hui-Seung,Ha, Sang-Do,Jeong, Seung-Weon,Jang, Mi,Kim, Jong-Chan The Korean Society for Applied Biological Chemistr 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.6

Gwamegi (semidry Pacific saury [Cololabis saira]) is a Korean food made by a traditional method of repeated freezing and de-freezing during winter. The present study aimed at developing predictive modeling of S. aureus growth on Gwamegi as a function of temperature ($10-35^{\circ}C$). Modified Gompertz, Baranyi, and logistic primary models were fitted to experimental values. Polynomial quadratic, nonlinear Arrhenius and square root models were selected as secondary models and analyzed using specific growth rate (${\mu}_{max}$) and lag time (${\lambda}$) values obtained from the primary models. Based on the optimized models derived from the Baranyi and square root equations for ${\mu}_{max}$, its $r^2$ and mean square error (MSE) were 0.991 and 0.00058, and bias factor ($B_f$) and accuracy factor ($A_f$) were 1.0087 and 1.0801, respectively. The logistic and polynomial quadratic equations for ${\lambda}$, its $r^2$ and MSE were 0.989 and 0.22834, $B_f$ and $A_f$ were 0.9742 and 1.0271, respectively. These predictive models can provide basic information for quantitative microbial risk assessment of Gwamegi and other processed semidried seafood.

Insulin-Secreting Cells from Human Eyelid-Derived Stem Cells Alleviate Type I Diabetes in Immunocompetent Mice

Kang, Hyun Mi,Kim, Jiyoung,Park, Seah,Kim, Jinyoung,Kim, Haekwon,Kim, Kyung Sik,Lee, Eun Jig,Seo, Sung Ig,Kang, Sung Goo,Lee, Jong-Eun,Lim, Hyunjung Wiley (John WileySons) 2009 Stem Cells Vol.27 No.8

<P>Various attempts have been made to develop stem cell-based therapy to alleviate type I diabetes using animal models. However, it has been a question whether human insulin produced from explanted cells is solely responsible for the normoglycemia of diabetic animals. In this study, we isolated neural crest-like stem cells from the human eyelid fat and examined their therapeutic potentials for diabetes. The human eyelid adipose-derived stem cells (HEACs) displayed characteristics of neural crest cells. Using a two-step culture condition combined with nicotinamide, activin, and/or GLP-1, we differentiated HEACs into insulin-secreting cells and examined in vivo effects of differentiated cells by transplantation experiments. Following differentiation in vitro, HEACs released insulin and c-peptide in a glucose-dependent manner. Upon their transplantation under kidney capsules of streptozotocin-treated immunocompetent mice, we observed normalization of hyperglycemia in 10 of 20 recipient mice until sacrifice after 2 months. Only the human, but not the mouse, insulin and c-peptide were detected in the blood of recipient mice. Removal of the kidneys transplanted with HEACs resulted in a sharp increase of blood glucose level. Removed kidney tissues showed distinct expression of various human genes including insulin, and colocalization of the human insulin and the human nuclear protein in many cells. However, they showed diminished or null expression of some immune-related genes. In conclusion, human insulin alone produced from eyelid-derived stem cells following differentiation into insulin-secreting cells and transplantation could normalize type I diabetes in mice.</P>

Expression of thermosensitive two-pore domain K⁺ channels in human keratinocytes cell line HaCaT cells

Kang, Dawon,Kim, Sung-Hee,Hwang, Eun-Mi,Kwon, Oh-Sang,Yang, Hae-Young,Kim, Eun-Sook,Choi, Tae Hyun,Park, Jae-Yong,Hong, Seong-Geun,Han, Jaehee Blackwell Publishing Ltd 2007 Experimental dermatology Vol.16 No.12

<P>Abstract: </P><P>Recent studies have shown that keratinocytes can sense temperature via thermo-transient receptor potential (TRP) channels. It is not known whether other thermosensitive ion channels such as TREK-1, TREK-2 and TRAAK (TREKs/TRAAK) that are members of the two-pore domain K<SUP>+</SUP> (K<SUB>2P</SUB>) channel family are expressed in human keratinocytes. Here, we identified the expression of TREKs/TRAAK in human keratinocytes-derived cell line HaCaT cells using RT-PCR, immunocytochemistry, Western blot analysis and patch-clamp technique. RT-PCR showed that all six K<SUB>2P</SUB> channels tested (TASK-1, TASK-3, TREK-1, TREK-2, TRAAK and TASK-2) were expressed in HaCaT cells, as well as in skin and dorsal root ganglion (DRG) of rat. The expression of TREKs/TRAAK mRNA identified by RT-PCR was further studied at the protein level. Using anti-TREK-1, -TREK-2 and -TRAAK, bands of ∼46, ∼60 and ∼43 kDa, respectively, were observed at plasma membrane of HaCaT cells. Immunostaining also showed that TREK-1, TREK-2 and TRAAK were expressed in all area of cells including plasma membrane. Whole-cell K<SUP>+</SUP> currents recorded from HaCaT cells were activated by arachidonic acid and heat. These results suggest that TREKs/TRAAK channels could act as thermosensors in human keratinocytes.</P>

Genistein protects the kidney from cisplatin-induced injury

Sung, Mi Jeong,Kim, Duk Hoon,Jung, Yu Jin,Kang, Kyung Pyo,Lee, Ae Sin,Lee, Sik,Kim, Won,Davaatseren, Munkhtugs,Hwang, Jin-Taek,Kim, Hyun-Jin,Kim, Myung Sunny,Kwon, Dae Young,Park, Sung Kwang International Society of Nephrology 2008 Kidney international Vol.74 No.12

Oxidative stress and inflammation contribute to the pathogenesis of cisplatin-induced nephrotoxicity. We found that genistein, a tyrosine kinase inhibitor with broad specificities, and which also has estrogen-like activity, had protective effects on cisplatin-induced renal injury in mice. Genistein significantly decreased reactive oxygen species production, the expression of intercellular adhesion molecule-1 and monocyte chemoattractant protein-1 proteins, as well as the translocation of the p65 subunit of nuclear factor-κB into the nucleus and the infiltration of macrophages, all of which were increased in the kidney by cisplatin treatment. Genistein also decreased cisplatin-induced apoptosis by regulating p53 induction in kidney. Genistein significantly reduced reactive oxygen species production in cisplatin-treated normal human kidney HK-2 cells. These studies show that genistein or similar compounds might be useful in prevention of cisplatin-induced renal injury.Kidney International (2008) 74, 1538–1547; doi:10.1038/ki.2008.409; published online 20 August 2008

Multiple Gastrointestinal Stromal Tumors: Clinicopathologic and Genetic Analysis of 12 Patients

Kang, Dae Young,Park, Cheol Keun,Choi, Jong Sang,Jin, So Young,Kim, Hyun Jung,Joo, Mee,Kang, Mi Seon,Moon, Woo Sung,Yun, Ki Jung,Yu, Eun Sil,Kang, Haeyun,Kim, Kyoung-Mee Lippincott Williams Wilkins, Inc. 2007 The American journal of surgical pathology Vol.31 No.2

Multiple gastrointestinal stromal tumors (GISTs) are extremely rare and usually associated with type 1 neurofibromatosis and familial GIST. The aim of this study was to investigate the clinical, phenotypic, and genetic characteristics of multiple GISTs to gain insights into their underlying pathogenesis and clinical behavior. Forty-seven paraffin blocks of multiple GISTs from 12 patients were analyzed. Genomic DNA was extracted from the tumor and normal mucosa and mutations for 4 exons of KIT gene and 3 exons of PDGFRA gene were determined. Among 12 patients with multiple GISTs, 5 were sporadic, 2 were familial with germline mutations of KIT gene, and 5 were associated with type 1 neurofibromatosis. All but 1 sporadic and familial multiple GISTs showed mutations of KIT gene shared by the same mutation on each GIST mass within a patient. But in 1 sporadic case, different types of KIT mutations were observed. Two familial multiple GIST cases showed diffuse involvement of the gastrointestinal tract with diffuse hyperplasia of interstitial cell of Cajal. Multiple GISTs associated with type 1 neurofibromatosis were located in the jejunum and harbored no mutations of KIT or PDGFRA. Different types of KIT gene mutation found in our case raise a possibility that recurrence of GISTs within a gastrointestinal tract may have a chance to be a rare occurrence of multiple primary GISTs instead of true recurrence. Multiple GISTs show unique clinical, phenotypic, and genotypic characteristics that are dependent on the particular underlying mechanisms, but the overall prognosis is favorable regardless of the numbers or phenotype of GISTs.