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윤제원(Je-Won Yoon),김영찬(Young-Chan Kim),김금모(Keum-Mo Kim),장강석(Kang-Seok Jang),구본성(Bon-Sung Ku),엄주용(Joo-Yong Eom) 한국소음진동공학회 2011 한국소음진동공학회 학술대회논문집 Vol.2011 No.10
The purpose of this study is to develop an air-passing soundproofing panel with more improved structure to reduce the CO2 emission and installation cost. To reduce the emission of CO2 ; it is suggested to choose low CO2 emission material relative to the aluminum and to reduce the materials by developing a specially designed air-passing soundproofing panel structure. First of all, we performed the flow analysis to predict the wind pressure according to the open angle of the air-passing soundproofing panel and the noise level analysis at the receiver point. To verify the simulation, a prototype of the soundproofing panel was made. The flow test in the wind tunnel and load test were performed. The economic evaluation for the installation of the air-passing soundproofing panel was performed and specifications of the installation was prepared. As the results of this research, it was verified that the wind load was reduced about 40% to that of the conventional one at 25m/s wind speed in the wind tunnel test. By applying the 4m span soundproofing wall with air-passing soundproofing panel and under the cost of 250 thousand won/m2 instead of the conventional 2m span panel, the installation cost will always be lowered than the conventional one in the combination of (60:40~50:50) conventional to air-passing soundproofing panel from the economic evaluation. The 20% reduction of CO2 was found by changing the 50% of aluminum soundproof panel to air-passing soundproofing panel.
Kang, Gum-Yong,Bang, Joo Young,Choi, Ae Jin,Yoon, Jeehyun,Lee, Won-Chul,Choi, Soyoung,Yoon, Soojin,Kim, Hyung Chan,Baek, Je-Hyun,Park, Hyung Soon,Lim, Hyunjung Jade,Chung, Hyewon American Chemical Society 2014 JOURNAL OF PROTEOME RESEARCH Vol.13 No.2
<P>Age-related macular degeneration (AMD) describes the progressive degeneration of the retinal pigment epithelium (RPE), retina, and choriocapillaris and is the leading cause of blindness in people over 50. The molecular mechanisms underlying this multifactorial disease remain largely unknown. To uncover novel secretory biomarkers related to the pathogenesis of AMD, we adopted an integrated approach to compare the proteins identified in the conditioned medium (CM) of cultured RPE cells and the exosomes derived from CM and from the aqueous humor (AH) of AMD patients by LC–ESI–MS/MS. Finally, LC–MRM was performed on the AH from patients and controls, which revealed that cathepsin D, cytokeratin 8, and four other proteins increased in the AH of AMD patients. The present study has identified potential biomarkers and therapeutic targets for AMD treatment, such as proteins related to the autophagy–lysosomal pathway and epithelial–mesenchymal transition, and demonstrated a novel and effective approach to identifying AMD-associated proteins that might be secreted by RPE in vivo in the form of exosomes. The proteomics-based characterization of this multifactorial disease could help to match a particular marker to particular target-based therapy in AMD patients with various phenotypes.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jprobs/2014/jprobs.2014.13.issue-2/pr400751k/production/images/medium/pr-2013-00751k_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/pr400751k'>ACS Electronic Supporting Info</A></P>
Comprehensive Review of Golgi Staining Methods for Nervous Tissue
Kang, Hee Won,Kim, Ho Kyu,Moon, Bae Hun,Lee, Seo Jun,Lee, Se Jung,Rhyu, Im Joo Korean Society of Microscopy 2017 Applied microscopy Vol.47 No.2
Golgi staining has been modified and developed since Camillo Golgi introduced the black reaction in 1873. This study focuses on the commonly used Golgi staining methods and presents comprehensive data regarding three Golgi staining methods along with their strong and weak points. The Golgi-Cox method uses mercuric chloride for brain tissue impregnation and is a reliable technique for analyzing the complete dendritic tree of cortical neurons. However, specimens tend to shrink during the staining steps. Recent combination of the Golgi-Cox method and immunofluorescence provides additional options for neuroscientists. Rapid Golgi staining requires osmium tetroxide for the post-fixation process. It homogenously stains whole structures of neurons and provides their detailed anatomical morphology. This staining is influenced by the age of the specimen, temperature of the laboratory, and duration of each procedure. The Golgi-Kopsch method uses formaldehyde and glutaraldehyde instead of osmium tetroxide and can be used regardless of the age of the specimen and the duration after fixation. This method is suitable for research using human brain fixed for a long time or for specimens obtained from old-aged animals. Selecting a Golgi staining protocol that is appropriate for the specimen type and research purpose is important to achieve best results.
Evaluation of Cytotoxicity for Immunity Rejection of US11, hDAF and FasL Transgene-Transfected Cells
Kang, Jung Won,Shin, Hyeon Yeong,Oqani, Reza K.,Lin, Tao,Lee, Jae Eun,Kim, So Yeon,Lee, Joo Bin,Jin, Dong Il The Korean Society of Animal Reproduction 2017 Reproductive & developmental biology Vol.41 No.3
Xenotransplantation is proposed as a solution to the problem of organ shortage. However, transplantation of xenogeneic organs induces an antigen-antibody reaction in ${\alpha}$-1,3-gal structure that are not present in humans and primates, and thus complement is also activated and organs die within minutes or hours. In this study, we used FasL gene, which is involved in the immune response of NK cell, and US11, which suppresses MHC Class I cell membrane surface expression, to inhibit cell mediated rejection in the interspecific immunity rejection, and also hDAF(CD55) was introduced to confirm the response to C3 complement. These genes were tranfeced into Korean native pig fetal fibroblasts using pCAGGS vector. And cytotoxicity of NK cell and human complement was confirmed in each cell line. The US11 inhibited the cytotoxicity of NK cell and, in addition, the simultaneous expression of US11 and Fas ligand showed excellent suppress to T-lymphocyte cytotoxicity, hDAF showed weak resistance to cytotoxicity of natural killer cell but not in CD8+ CTLs. Cytotoxicity study with human complement showed that hDAF was effective for reducing complement reaction. In this studies have demonstrated that each gene is effective in reducing immune rejection.
Kang, Seok-Jin,Jeong, Sang-Hee,Kim, Eun-Joo,Cho, Joon-Hyoung,Park, Young-Il,Park, Sung-Won,Shin, Hyo-Sook,Son, Seong-Wan,Kang, Hwan-Goo Princeton Scientific Publishers 2013 Cell biology and toxicology Vol.29 No.1
<P>Embryonic stem cell testing is an alternative model system to assess drug and chemical toxicities because of its similar developmental characteristics with in vivo embryogenesis and organogenesis. This study evaluated the toxicity of chemicals at specific developmental stages of mouse embryonic stem cell (ESC)-derived hepatic differentiation; hepatic progenitor cells (HPCs), and hepatocyte-like cells (HCs). The toxic effects of carbon tetrachloride (CCl(4)), 5-fluorouracil (5-FU), and arsanilic acid (Ars) were evaluated by measuring the expressions of Cytokeratin (CK18) and GATA binding protein 4 (GATA-4) and the activities of aspartate transaminase (AST), lactate dehydrogenase (LDH), and alkaline phosphatase (ALP) during the hepatic differentiation process. Non-toxic doses of three chemicals at a range of 25 to 500?μM for CCl(4), 12.5 to 800?nM for 5-FU and 6.25 to 400?mM for Ars were treated. In the CCl(4)-treated group, significant decreases (P?<?0.05) of the marker expression were observed by more than 300?μM from day 10 in CK18 and by more than 400?μM of CCl(4) from day 22 in GATA-4, respectively. However, both markers were decreased (P?<?0.01) by treatments of all doses at day 40. In the 5-FU-treated group, the expressions of two proteins were not affected by any of the doses at day 10 and 22, whereas the GATA-4 expression was decreased (P?<?0.05) by more than 400?nM of 5-FU at days 28 and 40. In the Ars-treated group, the CK18 expression was inhibited (P?<?0.05) by more than 100?mM of Ars at day 22 but showed a tendency to recover. Although the GATA-4 was inhibited by all doses at day 22, the inhibition of GATA-4 recovered at days 28 and 40. ALP activities of three chemicals were significantly increased (P?<?0.05) by a dose-dependent manner. The activities of AST and LDH were prone to be increased by more than 300?μM of CCl(4,) but not affected by all doses of 5-FU except for 800?nM of 5-FU in AST activities. In the Ars, the enzyme activities were significantly increased (P?<?0.05) by more than 50?μM of Ars in AST and more than 6.25?μM of Ars in LDH. The present results indicate that CCl(4) has a more toxic effect on HCs, whereas Ars is more toxic to HPCs. Additionally, in vitro alternative testing using ESC-derived HPCs and HCs could provide useful information on chemical toxicity during the hepatic differentiation process and could be a useful model system for assessing chemical hepatotoxicity.</P>
Case Reports : Disseminated Mycobacterium intracellulare Infection in an Immunocompetent Host
( Won Young Kim ),( Sun Joo Jang ),( Tae Jin Ok ),( Gwang Un Kim ),( Han Seung Park ),( Jae Chan Leem ),( Bo Hyoung Kang ),( Se Jeong Park ),( Dong Kyu Oh ),( Byung Ju Kang ),( Bo Young Lee ),( Won Ju 대한결핵 및 호흡기학회 2012 Tuberculosis and Respiratory Diseases Vol.72 No.5
Disseminated Mycobacterium avium complex (MAC) infection can occur in immunocompromised patients, and rarely in immunocompetent subjects. Due to the extensive distribution of the disease, clinical presentation of disseminated MAC may mimic malignancies, and thorough examinations are required in order to make accurate diagnosis. We report a case of disseminated Mycobacterium intracellulare disease in an immunocompetent patient, which involved the lung, lymph nodes, spleen, and multiple bones. F-18 fluorodeoxyglucose positron-emission tomography imaging showed multiple hypermetabolic lesions, which are suggestive of typical hematogenous metastasis. However, there was no evidence of malignancy in serial biopsies, and M. intracellulare was repeatedly cultured from respiratory specimens and bones. Herein, we should know that disseminated infection can occur in the immunocompetent subjects, and it can mimic malignancies.
Application study of PCR additives to improve the split peaks in direct PCR
Joo-Young Kim,Da-Hye Kim,Hyun-Chul Park,Ju Yeon Jung,Gang-Nam Jin,In-Kwan Hwang,Pil-Won Kang 한국분석과학회 2019 분석과학 Vol.32 No.4
Analysis techniques using DNA profiling are widely used in various fields including forensic science and new technologies such as the Direct PCR amplification method are being developed continuously in order to acquire the DNA profiles efficiently. However, it has a limits such as non-specific amplification according to the quality of crime scene evidence samples. Especially, split peaks caused by excessive DNA samples are one of the important factors that could cause the debate to allow researchers to interpret the DNA profile results. In this study, we confirmed the occurrence rate of split peaks in each STR (short tandem repeats) locus of the GlobalFilerTM kit and investigated the possibility of improving the split peaks using several PCR additives such as DMSO (dimethylsulfoxide), MgCl2, Betaine and Tween-20. As a result, we could make three groups according to the occurrence rate of split peaks in Direct PCR and it was confirmed that the ratio of split peaks could be reduced by DMSO (87.4 %), MgCl2 (84.5 %) and Betaine (86.1 %), respectively. These results indicate that PCR additives such as DMSO, MgCl2 and Betaine can be improve the split peaks in Direct PCR and thereby facilitate subsequently a successful DNA profile results.
( Kang Hee Ahn ),( Sang Soo Kim ),( Won Jin Kim ),( Jong Ho Kim ),( Yun Jeong Nam ),( Su Bin Park ),( Yun Kyung Jeon ),( Bo Hyun Kim ),( In Joo Kim ),( Yong Ki Kim ) 대한내과학회 2017 The Korean Journal of Internal Medicine Vol.32 No.5
Background/Aims: We evaluated whether serum bilirubin levels can predict the development of chronic kidney disease (CKD) in patients with type 2 diabetes mellitus (T<sub>2</sub>DM). Methods: This was a retrospective observational longitudinal study of patients presenting at the Pusan National University Hospital. A total of<sub>349</sub> patients with T<sub>2</sub>DM and preserved kidney function (estimated glomerular filtration rate ≥ 60 mL/min/1.73 m<sup>2</sup>) were enrolled. The main outcome was the development of CKD stage 3 or greater. The patients were divided into four groups according to the quartiles of the total serum bilirubin levels at baseline. Results: The group with the lowest range of total serum bilirubin level (Q<sub>1</sub>) showed the highest cumulative incidence of CKD stage 3 or greater than that of the other lower quartiles (Q<sub>1</sub> vs. Q<sub>4</sub>; hazard ratio [HR], 6.75; 95% confidence in-terval [CI], 1.54 to 29.47; p = 0.011). In multivariate analysis, the risk of developing CKD stage 3 or greater was higher in the second lowest quartile of the serum bili-rubin level than that in the highest quartile of the serum bilirubin level (Q<sub>2</sub> vs. Q<sub>4</sub>; HR, 9.36; 95% CI, 1.33 to 65.73; p = 0.024). In the normoalbuminuria subgroup (n = 236), multivariate analysis showed that the risk of developing CKD stage 3 or greater was higher in the lowest quartile of the serum bilirubin level than that in the highest quartile of the serum bilirubin level (Q1 vs. Q4; HR, 7.36; 95% CI, 1.24 to 35.82; p = 0.019). Conclusions: Serum bilirubin might be an early clinical marker for predicting the progression of CKD in patients with T<sub>2</sub>DM and preserved renal function.