Etiopathogenic role of ERK5 signaling in sarcoma: prognostic and therapeutic implications

Sánchez-Fdez Adrián,Matilla-Almazán Sofía,Del Carmen Sofía,Abad Mar,Arconada-Luque Elena,Jiménez-Suárez Jaime,Chinchilla-Tábora Luis Miguel,Ruíz-Hidalgo Mª José,Sánchez-Prieto Ricardo,Pandiella Atanas 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Sarcomas constitute a heterogeneous group of rare and difficult-to-treat tumors that can affect people of all ages, representing one of the most common forms of cancer in childhood and adolescence. Little is known about the molecular entities involved in sarcomagenesis. Therefore, the identification of processes that lead to the development of the disease may uncover novel therapeutic opportunities. Here, we show that the MEK5/ERK5 signaling pathway plays a critical role in the pathogenesis of sarcomas. By developing a mouse model engineered to express a constitutively active form of MEK5, we demonstrate that the exclusive activation of the MEK5/ERK5 pathway can promote sarcomagenesis. Histopathological analyses identified these tumors as undifferentiated pleomorphic sarcomas. Bioinformatic studies revealed that sarcomas are the tumors in which ERK5 is most frequently amplified and overexpressed. Moreover, analysis of the impact of ERK5 protein expression on overall survival in patients diagnosed with different sarcoma types in our local hospital showed a 5-fold decrease in median survival in patients with elevated ERK5 expression compared with those with low expression. Pharmacological and genetic studies revealed that targeting the MEK5/ERK5 pathway drastically affects the proliferation of human sarcoma cells and tumor growth. Interestingly, sarcoma cells with knockout of ERK5 or MEK5 were unable to form tumors when engrafted into mice. Taken together, our results reveal a role of the MEK5/ERK5 pathway in sarcomagenesis and open a new scenario to be considered in the treatment of patients with sarcoma in which the ERK5 pathway is pathophysiologically involved.

Two new species from the Mexican Pacific Slope and new distributional records of Onthophagus Latreille, 1802 (Coleoptera, Scarabaeidae)

Moctezuma Victor,Hernández Benjamín,Sánchez-Huerta José Luis,Lizardo Viridiana,Quiroz-Rocha Georgina Adriana,Navarrete-Heredia José Luis 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.1

Two new species of Onthophagus Latreille, 1802 from the Pacific Slope of Mexico are described and illustrated: Onthophagus gonzaloi Moctezuma, Hernández & Sánchez-Huerta, sp. nov., and Onthophagus yescaensis Moctezuma, Hernández & Sánchez-Huerta, sp. nov. New records for Onthophagus browni Howden & Cartwright, 1963 from the Mexican states of Jalisco (the southernmost locality known to date) and Zacatecas are provided. urn:lsid:zoobank.org:pub:51AA52C6-41BF-4A8B-9BC9-D78600EDB32E.

Bursectomy, Curettage, and Chemotherapy in Tuberculous Trochanteric Bursitis

Luis R. Ramos-Pascua,José A. Carro-Fernández,José A. Santos-Sánchez,Paula Casas Ramos,Luis J. Díez-Romero,Francisco M. Izquierdo-García 대한정형외과학회 2016 Clinics in Orthopedic Surgery Vol.8 No.1

We presented three patients with trochanteric tuberculosis and described the clinical and imaging findings of the infection. Histology revealed a necrotizing granulomatous bursitis and microbiology confirmed tuberculosis. All cases were successfully treated with bursectomy and curettage of the trochanteric lesion and antituberculous chemotherapy including isoniazid, pyrazinamide, rifampicin, and ethambutol.

DNA accumulation on single-anode microelectrode structures and its application in active microarray layout design

Javier Vázquez,JoséLuis Sánchez-Rojás 한국물리학회 2009 Current Applied Physics Vol.9 No.3

The present work investigates, using simulation computer tools and fluorescence microscopy experimental assays, the local distribution of short single-stranded oligonucleotide fragments accumulated on a single microelectrode after the switching of an external dc electrical stimulus for some seconds (up to 75 s). It contributes to fill the existing gap in active microelectronic DNA arrays, where due to the large number of microelectrodes contained in the devices, no or little attention has been given to the final coverage of the individual electrodes by the DNA probes transported onto them, and how it is influenced by the device geometry and other variables like the current magnitude and the transport time. All these parameters play a fundamental role in both the accumulation rate of DNA strands on a polarized electrode and the degree of symmetry of their final distribution, which is convenient to know prior to any active microarray layout design in order to optimize the device performance. The present work investigates, using simulation computer tools and fluorescence microscopy experimental assays, the local distribution of short single-stranded oligonucleotide fragments accumulated on a single microelectrode after the switching of an external dc electrical stimulus for some seconds (up to 75 s). It contributes to fill the existing gap in active microelectronic DNA arrays, where due to the large number of microelectrodes contained in the devices, no or little attention has been given to the final coverage of the individual electrodes by the DNA probes transported onto them, and how it is influenced by the device geometry and other variables like the current magnitude and the transport time. All these parameters play a fundamental role in both the accumulation rate of DNA strands on a polarized electrode and the degree of symmetry of their final distribution, which is convenient to know prior to any active microarray layout design in order to optimize the device performance.

Clean syngas production by gasification of lignocellulosic char: State of the art and future prospects

Rui Moreira,Fernando Bimbela,Noemí Gil-Lalaguna,José Luis Sánchez,António Portugal 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.101 No.-

Using lignocellulosic char instead of the original biomass avoids the need for costly cleaning andconditioning stages of the producer gasification gas. However, lignocellulosic char gasification has beenless extensively studied than gasification of lignocellulosic biomass, and a review of published works onthis topic was missing. In this review the present status of char gasification technologies and their futureprospects are critically discussed, including possible research opportunities. To date, most studies onchar gasification have been performed in thermogravimetric analyzers (TGA) or TGA-like experimentalsetups. The major setback of TGA and TGA-like equipment is that they do not mimic the actual reactionconditions occurring in gasification reactors, which impedes a direct extrapolation of thefindings duringthe scale-up of different gasification technologies. For this reason, in this literature review focus was puton studies undertaken in industrially relevant reactors, both in batch and continuous configurations. Overall, char gasification can be deemed a valid alternative for clean syngas production, contributing toan integral valorization of lignocellulosic residues within different biorefinery schemes. Of these, processintensification by microwave heating offers interesting opportunities for research and scaling-up, thoughefforts must be directed toward developing continuous microwave-assisted gasification processes.

Work Ability Index: Psychometric Testing in Aeronautical Industry Workers

González-Domínguez María Eugenia,Fernández-García Elena,Paloma-Castro Olga,González-López Regina María,Rivas Pérez María Paz,López-Molina Luis,García-Jiménez Jesús,Romero-Sánchez José Manuel 한국산업안전보건공단 산업안전보건연구원 2024 Safety and health at work Vol.15 No.1

Background The Work Ability Index (WAI) is an instrument that measures work ability. The wide dispersion of the WAI internationally has led to its adaptation for use in different countries. This study aimed to evaluate the psychometric properties of the Spanish version of the WAI. Methods A methodological design was used over an opportunistic sample of 233 workers in the aeronautical industry in Spain. Reliability was evaluated through internal consistency. Factorial validity, known groups, and convergent validity were tested. Results The Cronbach's alpha and item-total correlation indicated an adequate internal consistency. The confirmatory factor analysis, performed to evaluate the factorial validity, found adequate fit indices for a two-factor solution with a high correlation between the factors. Factor 1, “Subjectively estimated work ability and resources”, was composed of 3 subscales and factor 2, “Ill-health-related”, of 2 subscales. Subscales 4 and 6 had loading in both factors. Workers under 45 years of age obtained higher significant scores than older ones. Convergent validity was also evidenced since WAI was highly correlated with self-assessment of health status. Conclusions The Spanish version of the WAI has shown evidence of reliability and validity in this study, supporting its use in individual and collective health surveillance by occupational health professionals. The factorial solution that was found has previously been reported in another international context. However, further research is needed to resolve the discrepancies detected in the role of some subscales between other national and international studies. Background The Work Ability Index (WAI) is an instrument that measures work ability. The wide dispersion of the WAI internationally has led to its adaptation for use in different countries. This study aimed to evaluate the psychometric properties of the Spanish version of the WAI. Methods A methodological design was used over an opportunistic sample of 233 workers in the aeronautical industry in Spain. Reliability was evaluated through internal consistency. Factorial validity, known groups, and convergent validity were tested. Results The Cronbach's alpha and item-total correlation indicated an adequate internal consistency. The confirmatory factor analysis, performed to evaluate the factorial validity, found adequate fit indices for a two-factor solution with a high correlation between the factors. Factor 1, “Subjectively estimated work ability and resources”, was composed of 3 subscales and factor 2, “Ill-health-related”, of 2 subscales. Subscales 4 and 6 had loading in both factors. Workers under 45 years of age obtained higher significant scores than older ones. Convergent validity was also evidenced since WAI was highly correlated with self-assessment of health status. Conclusions The Spanish version of the WAI has shown evidence of reliability and validity in this study, supporting its use in individual and collective health surveillance by occupational health professionals. The factorial solution that was found has previously been reported in another international context. However, further research is needed to resolve the discrepancies detected in the role of some subscales between other national and international studies.