Rapid quantification of viable spore used in healing concrete cracks by a simple spectrophotometric method

Jinlong Zhang,Jingkun Lu,Bing Liu,Qiuyue Liu,Fan Jin,Miaojun Zhang,Yerong Liu,Yujun Song,Chenhui Dong,Wanyi Zhang,Ningxu Han,Xu Deng,Feng Xing 한양대학교 세라믹연구소 2019 Journal of Ceramic Processing Research Vol.20 No.S1

Quantification of viable spores is a time taking task due to the lack of rapid, efficient and accurate methods. This studypresented a simple spectrophotometric method for the detection of viable spores based on spore’s property of losing refractivityduring the germination process. By comparison of the results obtained by both spectrophotometric method and colonycounting method, a good linear correlation (R2 = 0.99) was achieved between viable spore concentration and OD loss underappropriate conditions. To avoid interference from ungerminable spores and vegetative cells, a turbidity complementationstrategy of keeping the initial concentration of spore suspensions at the same and relatively lower level was required. Thecalibration equation developed could be used to predict the viable spore yield produced in a series of fermentation experiments. The experimental results proved that this novel spectrophotometric method was sensitive, rapid, and easy to performcompared to conventional colony counting method.

Impacts of organic content and pH on consolidation of clayey dredger fill by vacuum preloading method

LianSheng Tang,Jing Song,HaoKun Chen,Ya Wang,JinLong Yin,JinLong Ye 한국지질과학협의회 2017 Geosciences Journal Vol.21 No.5

The vacuum preloading method combining prefabricated vertical drains (PVDs) and surcharge preloading from vacuum pressure is widely used in the improvement of dredger fill foundation. However, a clogging effect arises within the soils around the PVDs during the vacuum preloading, which significantly affects the soil consolidation. Previous studies indicated that the organic matter and soil pH circumstance influence the soil-water interaction and soil microstructure. In order to investigate the influences of organic matter and soil pH on the consolidation of clayey dredger fill, a series of small scale vacuum preloading tests was conducted on soils with different organic contents and soil pH values. Subsequently, Scanning Electron Microscopy observation test and vane shear test were carried out to evaluate the effect of vacuum preloading consolidation. The results showed that a low organic content or a high soil pH is beneficial for soil consolidation by vacuum preloading method, resulting in a more compact microstructure and a greater vane strength. There are two actions on the organic matter during the vacuum preloading consolidation, namely, material migration and organic matter dissolution. The organic matter dissolution effect is predominate at soil pH > 9.1, so that the consolidation effect is improved. However, the material migration effect is predominate at pH = 8.1, clay particles and organic matter can be transported with the water flow and redeposit near the drain pipes, resulting in a clogging effect. Moreover, a higher pH can induce the change of microstructure from flocculated association to deflocculated and dispersed association of clay particles, which is positive to soil consolidation. The results in this paper may offer helpful references for engineering practice.

High-performance ZIF-302 mixed-matrix membranes for efficient CO2 capture

Junfeng Qian,Eryue Song,Haiqian Lian,Jinlong Jiang,Chongqing Wang,Yichang Pan 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.4

High-performance mixed matrix membranes (MMMs) were developed to assist in controlling global warmingby capturing carbon dioxide (CO2) from moist flue gas. However, the separation performance of MMMs has alwaysbeen limited by gas permeability and long-term operating stability under humid conditions. ZIF-302 is a novel chabazite(CHA) topology structure with hydrophobic ligand. Herein, uniform ZIF-302 nanocrystals were doped into theP84 polymer matrix to synthesize different content of defect-free ZIF-302/P84 MMMs for CO2/N2 separation. A significantcombination of gas permeability and separation factors was found in MMMs with a ZIF-302 packing load of 30wt%. The gas permeability of CO2 and the separation factor of CO2/N2 were significantly increased to 5.2 Barrers and46, respectively, which breaks the trade-off between permeability and selectivity of the polymer membrane. In addition,the long-term operation stability showed that the separation performance of ZIF-302/P84 MMMs for CO2/N2 was maintainedmore than 30 h at 3 bar and 60 oC. The main characteristics of the MMMs prepared in this paper include the combinationof favorable structural stability under humid conditions and unaffected CO2/N2 gas separation performance.

Association between Glutathione S-Transferase T1, M1, and P1 Genotypes and the Risk of Colorectal Cancer

Ning Cong,Lisheng Liu,Ying Xie,Wenbo Shao,Jinlong Song 대한의학회 2014 Journal of Korean medical science Vol.29 No.11

Glutathione S-transferases (GSTs) are enzymes which play an important role in theneutralization of toxic compounds and eradication of electrophilic carcinogens. Geneticpolymorphisms within the genes encoding for GSTs may therefore cause variations in theirenzyme activity, which may in turn influence the interindividual susceptibility to cancers. In this study, we aimed to investigate the association between genetic polymorphisms ofGSTT1, GSTM1, and GSTP1 and the risk of colorectal cancer (CRC) in 264 cases and 317controls in a Chinese population. Genotyping was performed by using multiplex PCR (forGSTT1 and GSTM1) and PCR-RFLP (for GSTP1) methods. The association between thepolymorphic genotypes and CRC risk was evaluated by deriving odds ratios (ORs) and 95%confidence intervals (CIs) using unconditional logistic regression analysis. Our resultsshowed that individuals with GSTT1 and GSTM1 null genotypes exhibited a higher risk ofCRC (GSTT1, OR,1.66; 95% CI, 1.20-2.31, P = 0.003; GSTM1, OR,1.57; 95% CI,1.13-2.18, P = 0.007), while no association was observed for GSTP1 (Pheterozygous = 0.790 or Pvariant= 0.261). Furthermore, individuals who simultaneously carried the null genotypes forboth GSTT1 and GSTM1 showed a stronger risk association (OR, 1.95; 95% CI, 1.33-2.85;P < 0.001). In conclusion, the GSTT1 and GSTM1 polymorphisms, but not GSTP1, maymodulate the CRC risk among Chinese.

<i>Hymenobacter ruber</i> sp. nov., isolated from grass soil

Jin, Long,Lee, Hyung-Gwan,Kim, Song-Gun,Lee, Keun Chul,Ahn, Chi-Yong,Oh, Hee-Mock International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.3

<P>A taxonomic study using a polyphasic approach was performed on a Gram-stain-negative, aerobic, non-motile, non-spore-forming, rod-shaped bacterium, designated strain PB156<SUP>T</SUP>, isolated from grass soil. Comparative 16S rRNA gene sequence studies showed that the isolate was clearly affiliated with the phylum <I>Bacteroidetes</I>, and most closely related to <I>Hymenobacter soli</I> PB17<SUP>T</SUP>, <I>Hymenobacter antarcticus</I> VUG-A42aa<SUP>T</SUP> and <I>Hymenobacter glaciei</I> VUG-A130<SUP>T</SUP>, showing 96.4, 96.2 and 95.9 % 16S rRNA gene sequence similarity, respectively, while all other species of the genus shared only 89.3–95.2 % similarity. The main polyamine present was <I>sym</I>-homospermidine. The predominant menaquinone was MK-7. The major fatty acids were C<SUB>15 : 0</SUB> iso, summed feature 3 (C<SUB>16 : 1</SUB>ω6<I>c</I> and/or C<SUB>16 : 1</SUB>ω7<I>c</I>/<I>t</I>), C<SUB>16 : 1</SUB>ω5<I>c</I> and C<SUB>15 : 0</SUB> anteiso. The G+C content of the genomic DNA of strain PB156<SUP>T</SUP> was 61.7 mol%. The combined genotypic and phenotypic data supported the conclusion that strain PB156<SUP>T</SUP> represents a novel species of the genus <I>Hymenobacter</I>, for which the name <I>Hymenobacter ruber</I> sp. nov. is proposed. The type strain is PB156<SUP>T</SUP> ( = KCTC 32477<SUP>T</SUP> = JCM 19433<SUP>T</SUP>).</P>

Transobturator four-arms mesh in the surgical management of cystocele: a long-term follow-up

Jin Long,Chung Lyul Lee,Seung Woo Yang,Ji Yong Lee,Jae Geun Lee,Ki Hak Song,Jae Sung Lim,Jong Mok Park,Yong Gil Na,Geon Gil,Ju Hyun Shin 한국운동재활학회 2021 JER Vol.17 No.1

Use of Lipid Extracts from Various Oil Grains to Supply Dietary Omega-3 Fatty Acids for Dairy Foods - A Preliminary Study

Jin, Long,Sim, Jeong Seok,Song, Kwang-Young,Kim, Dong-Hyeon,Chon, Jung-Whan,Kim, Hyunsook,Seo, Kun-Ho Korean Society of Dairy Science and Biotechnology 2018 Journal of Dairy Science and Biotechnology (JMSB) Vol.36 No.1

The objective of this research was to determine the grain oil most suitable for the production of omega-3 fatty acids (FAs) in China, in order to supply dietary omega-3 FAs. This study focused on the nutritional significance of omega-3 FAs and the wide search of sources of dietary omega-3 FA from oil grains produced in China. Five oil grains produced in the Yanbian region, namely sesame, flax, peanut, soybean, and corn, were selected and analyzed for their fat content and FA composition. Results showed that the lipid content in sesame, flax, and peanut, which was more than 30%, was higher than that in soybean and corn. The polyunsaturated FA content was found to be directly proportional to omega-3 FA content. Flax showed a considerably higher omega-3 FA content (53.43%) than other samples, suggesting its potential as a source of dietary omega-3 FA. The optimal daily intake of flax for males and females was determined to be 2.99 g (over one table spoon) and 2.06 g (nearly one table spoon), respectively. Flax showed the lowest ratio of omega-6 and omega-3 FA (0.32%), which can help reduce this ratio in the human body; the intake of equal amounts of flax and corn could achieve an optimum ratio of 1:1. These results can prove to be valuable in the preparation of various functional dairy foods in the future.