Sensitive Colorimetric Sensor for Lead Ions and VOCs Based on Histidine-Functionalized Polydiacetylene

Guang Yang,Ziwei Nie,Siyu Zhang,Zhaoliang Ge,Jiayin Zhao,Jiuru Zhang,Bin Li 한국고분자학회 2020 Macromolecular Research Vol.28 No.13

Due to the high toxicity of lead ions (Pb2+) and volatile organic compounds (VOCs) to human health and the environment, increasing attention has been paid by scientists to the development of simple, flexible and sensitive sensors or methods that are capable of tracing Pb2+ ions or VOCs with colorimetric visualization. In this study, the fabrication of colorimetric sensors based on histidine-functionalized diacetylene (His-DA), chromatic π-conjugated liposomes, for tracing Pb2+ ions was described. With a suitable molar ratio (4:1) between the10,12-pentacosadiynoic acid (PCDA) and His-DA monomers, the histidine units of the hybrid liposomes could rationally interrupt the efficient backbone length of polydiacetylene (PDA) chains, which causes the probe to be highly sensitive and selective for colorimetric visualization of tracing Pb2+ ions. Moreover, the His-PDA films also displayed sensitivity to volatile organic solvents or vapors, which could promote vivid color changes from blue to purple or pink. These interesting findings indicate that histidine-functionalized diacetylene may offer a promising way to design smart devices for real applications of sensing or tracing hazardous substances in the future.

Microvascular Myocardial Ischemia in Patients With Diabetes Without Obstructive Coronary Stenosis and Its Association With Angina

Yu Yarong,Yang Wenli,Dai Xu,Yu Lihua,Lan Ziting,Ding Xiaoying,Zhang Jiayin 대한영상의학회 2023 Korean Journal of Radiology Vol.24 No.11

Objective: To investigate the incidence of microvascular myocardial ischemia in diabetic patients without obstructive coronary artery disease (CAD) and its relationship with angina. Materials and Methods: Diabetic patients and an intermediate-to-high pretest probability of CAD were prospectively enrolled. Non-diabetic patients but with an intermediate-to-high pretest probability of CAD were retrospectively included as controls. The patients underwent dynamic computed tomography-myocardial perfusion imaging (CT-MPI) and coronary computed tomography angiography (CCTA) to quantify coronary stenosis, myocardial blood flow (MBF), and extracellular volume (ECV). The proportion of patients with microvascular myocardial ischemia, defined as any myocardial segment with a mean MBF ≤ of 100 mL/min/100 mL, in patients without obstructive CAD (Coronary Artery Disease–Reporting and Data System [CAD-RADS] grade 0–2 on CCTA) was determined. Various quantitative parameters of the patients with and without diabetes without obstructive CAD were compared. Multivariable analysis was used to determine the association between microvascular myocardial ischemia and angina symptoms in diabetic patients without obstructive CAD. Results: One hundred and fifty-two diabetic patients (mean age: 59.7 ± 10.7; 77 males) and 266 non-diabetic patients (62.0 ± 12.3; 167 males) were enrolled; CCTA revealed 113 and 155 patients without obstructive CAD, respectively. For patients without obstructive CAD, the mean global MBF was significantly lower for those with diabetes than for those without (152.8 mL/min/100 mL vs. 170.4 mL/min/100 mL, P < 0.001). The mean ECV was significantly higher for diabetic patients (27.2% vs. 25.8%, P = 0.009). Among the patients without obstructive CAD, the incidence of microvascular myocardial ischemia (36.3% [41/113] vs. 10.3% [16/155], P < 0.001) and interstitial fibrosis (69.9% [79/113] vs. 33.3% [8/24], P = 0.001) were significantly higher in diabetic patients than in the controls. The presence of microvascular myocardial ischemia was independently associated with angina symptoms (adjusted odds ratio = 3.439, P = 0.037) in diabetic patients but without obstructive CAD. Conclusion: Dynamic CT-MPI + CCTA revealed a high incidence of microvascular myocardial ischemia in diabetic patients without obstructive CAD. Microvascular myocardial ischemia is strongly associated with angina.

Assessment of Myocardial Bridge by Cardiac CT: Intracoronary Transluminal Attenuation Gradient Derived from Diastolic Phase Predicts Systolic Compression

Mengmeng Yu,Yang Zhang,Yuehua Li,Minghua Li,Wenbin Li,Jiayin Zhang 대한영상의학회 2017 Korean Journal of Radiology Vol.18 No.4

Objective: To study the predictive value of transluminal attenuation gradient (TAG) derived from diastolic phase of coronary computed tomography angiography (CCTA) for identifying systolic compression of myocardial bridge (MB). Materials and Methods: Consecutive patients diagnosed with MB based on CCTA findings and without obstructive coronary artery disease were retrospectively enrolled. In total, 143 patients with 144 MBs were included in the study. Patients were classified into three groups: without systolic compression, with systolic compression < 50%, and with systolic compression ≥ 50%. TAG was defined as the linear regression coefficient between intraluminal attenuation in Hounsfield units (HU) and length from the vessel ostium. Other indices such as the length and depth of the MB were also recorded. Results: TAG was the lowest in MB patients with systolic compression ≥ 50% (-19.9 ± 8.7 HU/10 mm). Receiver operating characteristic curve analysis was performed to determine the optimal cutoff values for identifying systolic compression ≥ 50%. The result indicated an optimal cutoff value of TAG as -18.8 HU/10 mm (area under curve = 0.778, p < 0.001), which yielded higher sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic accuracy (54.1, 80.5, 72.8, and 75.0%, respectively). In addition, the TAG of MB with diastolic compression was significantly lower than the TAG of MB without diastolic compression (-21.4 ± 4.8 HU/10 mm vs. -12.7 ± 8 HU/10 mm, p < 0.001). Conclusion: TAG was a better predictor of MB with systolic compression ≥ 50%, compared to the length or depth of the MB. The TAG of MB with persistent diastolic compression was significantly lower than the TAG without diastolic compression.

A General Encryption Algorithm for Different Format Videos

Hao Li,Cheng Yang,Jiayin Tian,Jianbo Liu 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.10

With the rapid development of social network, more and more information is being presented in the form of multimedia, so the security of multimedia files, especially video files, has been capturing the attention of the researchers. Video encryption is widely applied to the DRM system, video conference, medical and military fields and so on. A general video encryption algorithm is proposed to solve the problem that the existing multiple formats (compression formats and container formats) of video requires a unified encryption scheme. Meanwhile, the proposed scheme can not only provide variable levels of security, but also have good performance of compression efficiency and computational complexity, which can ensure that the encrypted video data can be operation.

Modularized bioceramic scaffold/hydrogel membrane hierarchical architecture beneficial for periodontal tissue regeneration in dogs

Yingming Wei,Zhongxiu Wang,Jiayin Han,Xiaojian Jiang,Lihong Lei,Xianyan Yang,Weilian Sun,Zhongru Gou,Lili Chen 한국생체재료학회 2022 생체재료학회지 Vol.26 No.4

Background: Destruction of alveolar bone and periodontal ligament due to periodontal disease often requires surgical treatment to reconstruct the biological construction and functions of periodontium. Despite significant advances in dental implants in the past two decades, it remains a major challenge to adapt bone grafts and barrier membrane in surgery due to the complicated anatomy of tooth and defect contours. Herein, we developed a novel biphasic hierarchical architecture with modularized functions and shape based on alveolar bone anatomy to achieve the ideal outcomes. Methods: The integrated hierarchical architecture comprising of nonstoichiometric wollastonite (nCSi) scaffolds and gelatin methacrylate/silanized hydroxypropyl methylcellulose (GelMA/Si-HPMC) hydrogel membrane was fabricated by digital light processing (DLP) and photo-crosslinked hydrogel injection technique respectively. The rheological parameters, mechanical properties and degradation rates of composite hydrogels were investigated. L-929 cells were cultured on the hydrogel samples to evaluate biocompatibility and cell barrier effect. Cell scratch assay, alkaline phosphatase (ALP) staining, and alizarin red (AR) staining were used to reveal the migration and osteogenic ability of hydrogel membrane based on mouse mandible-derived osteoblasts (MOBs). Subsequently, a critical-size one-wall periodontal defect model in dogs was prepared to evaluate the periodontal tissue reconstruction potential of the biphasic hierarchical architecture. Results: The personalized hydrogel membrane integrating tightly with the nCSi scaffolds exhibited favorable cell viability and osteogenic ability in vitro, while the scratch assay showed that osteoblast migration was drastically correlated with Si-HPMC content in the composite hydrogel. The equivalent composite hydrogel has proven good physiochemical properties, and its membrane exhibited potent occlusive effect in vivo; meanwhile, the hierarchical architectures exerted a strong periodontal regeneration capability in the periodontal intrabony defect models of dogs. Histological examination showed effective bone and periodontal ligament regeneration in the biomimetic architecture system; however, soft tissue invasion was observed in the control group. Conclusions: Our results suggested that such modularized hierarchical architectures have excellent potential as a next-generation oral implants, and this precisely tuned guided tissue regeneration route offer an opportunity for improving periodontal damage reconstruction and reducing operation sensitivity.

Lightweight Key Distribution Protocol for Streaming Media DRM

Hao Li,Tang Xuyue,Jiayin Tian,Jianbo Liu,Cheng Yang 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.9

In the daily-real systems, protecting the data only is far less enough. The process about the generation, distribution, storage, and the revocation of the key is the core problem in the system-security consideration. If the management of the key is insecure, attackers could easily get the key used in the encryption steps to the context, leading to useless processing in the encryption no matter how secure the system be. Unlike the period of validity for the different key, the key can easily be attacked by various methods due to its distribution through the complex net links, which would bring about the threatening of the security. In this paper, we analyze three models of the key distribution, associated with the actual applications for the encryption in the hypermedia video stream. Besides, we raise up a new protocol based on the IKEV2 distribution protocol and evaluate the stability when it suffer the Man-in –the-Middle Attacks, DoS and Replay attacks.

Chemokine Signaling Pathway Involved in CCL2 Expression in Patients with Rheumatoid Arthritis

Lin Zhang,Changyi Li,Min Yu,Jiayin Deng,Xing Lv,Jun Liu,Yu Xiao,Wenjie Yang,Yuru Zhang 연세대학교의과대학 2015 Yonsei medical journal Vol.56 No.4

Purpose: Rheumatoid arthritis (RA) is an inflammatory joint disorder, the progressionof which leads to the destruction of cartilage and bone. Chemokines are involvedin RA pathogenesis. In this study, we investigated the chemokine signaling pathway associated with CCL2 in peripheral blood (PB) and synovial tissues (ST) of RA patients based on our previous work about chemokine signaling pathway involvedin the activation of CCL2 production in collagen-induced arthritis rat ST. Materials and Methods: Total RNA was isolated from PB leukocytes and synoviumof the knee joint in both RA patients and control populations. Real-time polymerasechain reaction was used to determine CCL4, CCR5, c-Jun, c-Fos, and CCL2 expressions. Serum level of CCL2 was assessed by enzyme-linked immunosorbent assay, and the production of CCL2 in ST was analyzed immunohistochemically. Results: The expressions of CCL4, CCR5, c-Jun, c-Fos, and CCL2 messenger RNA in RA patients were significantly higher than those in healthy controls, both in ST and on PB leukocyte. Serum CCL2 levels were elevated in RA patients. Histological examination of rheumatoid joints revealed extensive CCL2 expression in RA ST. Conclusion: CCL2, CCL4, c-Jun, c-Fos, and CCR5 may play an important role in the recruitment of PB leukocytes into the RA joints. These data provide evidence that the chemokine signaling pathway is involved in CCL2 expression in RA patient tissues, which may contribute to chronic inflammation associated with RA. Targetingthis signaling pathway may provide a novel therapeutic avenue in RA.

A R2R3-type MYB transcription factor gene from soybean, GmMYB12, is involved in flavonoids accumulation and abiotic stress tolerance in transgenic Arabidopsis

Feibing Wang,Xuqin Ren,Fan Zhang,Mingyang Qi,Huiyun Zhao,Xinhong Chen,Yuxiu Ye,Jiayin Yang,Shuguang Li,Yi Zhang,Yuan Niu,Qing Zhou 한국식물생명공학회 2019 Plant biotechnology reports Vol.13 No.3

The R2R3-type MYB transcription factors have been shown to increase flavonoids accumulation by regulating the expression of key enzyme genes related to flavonoid biosynthesis pathway. However, the roles and underlying mechanisms of the soybean GmMYB12 gene in regulation of flavonoids accumulation and tolerance to abiotic stresses are rarely known. In the present study, the GmMYB12 gene was isolated and its function was characterized. Sequence and yeast one-hybrid analyses showed that GmMYB12 contained two MYB domains and belonged to R2R3-MYB protein with transactivation activity. Subcellular localization analysis in onion epidermal cells indicated that GmMYB12 was localized to the nucleus. Overexpression of GmMYB12 increased the production of downstream flavonoids and the expression of related genes in the flavonoid biosynthesis pathway. It also improved resistance to salt and drought stresses during seed germination, root development, and growing stage. Further component and enzymatic analyses showed significant increases of proline content, pyrroline-5-carboxylate synthase (P5CS), superoxide dismutase (SOD), and peroxidase (POD) activities, as well as significant reduction of H2O2 and malonaldehyde (MDA) content under salt and drought stresses in transgenic plants. Meanwhile, the expression level of AtP5CS, AtSOD and AtPOD genes was up-regulated against salt and drought stresses. Together, our finding indicated that changing the expression level of GmMYB12 in plants alters the accumulation of flavonoids and regulates plantlet tolerance to abiotic stress by regulating osmotic balance, protecting membrane integrity and maintaining ROS homeostasis. The GmMYB12 gene has the potential to be used to increase the content of valuable flavonoids and improve the tolerance to abiotic stresses in plants

Nomograms to Predict the Individual Survival of Patients with Solitary Hepatocellular Carcinoma after Hepatectomy

( Junyi Shen ),( Linye He ),( Chuan Li ),( Tianfu Wen ),( Weixia Chen ),( Changli Lu ),( Lvnan Yan ),( Bo Li ),( Jiayin Yang ) 대한간학회 2017 Gut and Liver Vol.11 No.5

Background/Aims: Solitary hepatocellular carcinoma (HCC) is a subgroup of HCCs. We aimed to establish nomograms for predicting the survival of solitary HCC patients after hepatectomy. Methods: A total of 538 solitary HCC patients were randomly classified into training and validation sets. A Cox model was used to identify predictors of overall survival (OS) in the training set. A nomogram was generated based on these predictors and was validated using the validation set. Results: Tumor size, microvascular invasion, and major vascular invasion were significantly associated with OS in the training set. Nomograms were developed based on these predictors in the multivariate analysis. The C-index was 0.75 for the OS nomogram and 0.72 for the recurrence-free sur-vival nomogram. Compared to the index of conventional stag-ing systems for predicting survival (0.71 for Barcelona Clinic Liver Cancer, 0.66 for the seventh American Joint Committee on Cancer, 0.68 for Cancer of the Liver Italian Program, and 0.70 for Hong Kong Liver Cancer), the index of the OS nomo-gram was significantly higher. Moreover, the calibration curve fitted well between the predicted and observed survival rate. Similarly, in the validation set, the nomogram discrimination was superior to those of the four staging systems (p<0.001). Conclusions: The nomograms demonstrated good discrimi-nation performance in predicting 3- and 5-year survival rates for solitary HCCs after hepatectomy. (Gut Liver 2017;11:684- 692)