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산업단지 및 일반생활 지역의 공기 중 톨루엔에 대한 개인노출 및 생체시료의 상관성 분석
이병준(Byoungjun Lee),허 정(Jung Heo),정다영(Dayoung Jung),김순신(Sunshin Kim),류현수(Hyeon-Su Ryu),최민지(Min-Ji Choi),솨이지엔페이(Jian-Fei Shuai),임성국(Sung-Guk Im),양원호(Wonho Yang) 한국환경보건학회 2017 한국환경보건학회지 Vol.43 No.4
Objectives: The purpose of this study was to assess the correlation relationship between personal exposure and urinary hippuric acid in biological monitoring for airborne toluene in an industrial complex and in general environments. Methods: Personal exposure to toluene and its metabolite, hippuric acid, in urine were simultaneously measured in occupational environments area near an industrial complex and in general environments. The study subjects were divided into three types: 137 workers who use organic solvents in the workplace, 210 residents living near a dyeing industrial complex, and 379 residents living in general environments. The toluene exposures of workers and residents were measured by a passive sampler for four days. The urine of participants was sampled when the passive samplers of personal exposure were collected. Results: The toluene and hippuric acid concentrations of workers were the highest, followed by the concentrations of residents living near a dyeing industrial complex and residents living in general environments. The coefficient of correlation between the concentrations of toluene and hippuric acid among workers was 0.749 (p<0.01) in the workplace. On the contrary, correlations between the concentrations of toluene and hippuric acid among residents living near a dyeing industrial complex and residents living in general environments were all not significant. The relationship between the concentrations of hippuric acid and toluene in three types could be described by the exponential growth model. Conclusions: This study analyzed the relationships between toluene exposure and the concentrations of hippuric acid in urine in high, middle, and low exposure environments, and could be described by the exponential growth model.
Ryu, Min Ju,Kang, Kyoung Ah,Piao, Mei Jing,Kim, Ki Cheon,Zheng, Jian,Yao, Cheng Wen,Cha, Ji Won,Hyun, Chang Lim,Chung, Ha Sook,Park, Jong Cook,Cho, Suk Ju,Hyun, Jin Won Springer 2014 In vitro cellular & developmental biology Animal Vol.50 No.6
<P>The cytoprotective mechanism of 7, 8-dihydroxyflavone (DHF) against oxidative stress-induced cell damage with respect to its stimulatory effect on the expression of heme oxygenase-1 (HO-1), a potent antioxidant enzyme, was investigated in the present study. Up-regulation of HO-1 expression by DHF was both dose and time dependent in lung fibroblast V79-4 cells. DHF also increased the protein expression level of the transcription factor nuclear factor erythroid-2-related factor 2 (Nrf2), and induced the translocation of Nrf2 from the cytosol into the nucleus, leading to elevated HO-1 expression. The siNrf2 RNA-transfection attenuated HO-1 expression induced by DHF treatment. In addition, DHF induced the activation of extracellular signal-regulated kinase (ERK), while U0126 (a specific pharmacological inhibitor of ERK kinase) abrogated DHF-activated Nrf2 and HO-1 expression. This suggests that DHF increased the levels of Nrf2 and HO-1 via ERK-dependent pathways. Furthermore, DHF significantly prevented the reduction of cell viability in response to oxidative stress; however, U0126 attenuated the protective effect of DHF. Taken together, these results demonstrate that DHF protected cells from oxidative stress via the activation of an ERK/Nrf2/HO-1 signaling pathway.</P>
Role of metallothionein 1E in the migration and invasion of human glioma cell lines.
Ryu, Hyang-Hwa,Jung, Shin,Jung, Tae-Young,Moon, Kyung-Sub,Kim, In-Young,Jeong, Young-Il,Jin, Shu-Guang,Pei, Jian,Wen, Min,Jang, Woo-Yeol Lychnia 2012 International journal of oncology Vol.41 No.4
<P>Metallothionein 1E (MT1E) has been found to be highly expressed in motile cell lines. We investigated whether MT1E actually modulates the migration and invasion of human glioma cell lines and the types of factors that have an effect on MT1E. RNA differential display was performed using Genefishing technology in the human glioma cell lines U343MG-A, U87MG and U87MG-10'; the results were validated by RT-PCR and northern blot analysis, in order to detect possible genetic changes as the determining factors for migration ability in malignant glioma. MT1E was identified in U87MG, a highly motile cell line. The migration and invasion abilities of human glioma cell lines, and MT1E transfectants were investigated using simple scratch testing and Matrigel invasion assays. Morphological and cytoskeletal (actin, vimentin) changes were documented by light and confocal microscopy. The expression of MT1E in four glioma cell lines was assessed by RT-PCR and western blotting. In addition, the effects of MT1E on the activity of the NF-κB p50/p65 transcription factor, MMP-2 and -9 were examined by western blotting and zymography. The endogenous MT1E expression in the human glioma cell lines was statistically correlated with their migratory abilities and invasion. The U87-MT-AS cells became more round and had decreased stress fibers, compared with the U87MG cells. Endogenous MT1E expression in the four human glioma cell lines was directly correlated with migration. Two antisense MT1E-transfected cell lines showed decreased NF-κB p50 translocation into the nucleus, which led to decreased activity of MMP-9 in conditioned media. It may be postulated that MT1E can enhance the migration and invasion of human glioma cells by inducing MMP-9 inactivation via the upregulation of NF-κB p50.</P>
Jian Pei,Kyung-Sub Moon,SangO Pan,Kyung-Hwa Lee,Hyang-Hwa Ryu,Tae-Young Jung,In-Young Kim,Woo-Yeol Jang,Chae-Hun Jung,Shin Jung 대한뇌종양학회 2014 Brain Tumor Research and Treatment Vol.2 No.1
Background To investigate the molecular basis for invasion of malignant gliomas, proteomic analysis approach was carried out using two human glioma cell lines, U87MG and U343MG-A that demonstrate different motility and invasiveness in in vitro experiments. Methods High-resolution two-dimensional gel electrophoresis and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry analysis were performed. Results Nine distinct protein spots that were recognized with significant alteration between the two cell lines. Five of these protein spots were up-regulated in U87MG and four were up-regulated in U343MG-A. Conclusion Among these proteins, cathepsin D was shown to be one of the important proteins which are related with glioma invasion. However, further studies are necessary to reveal the exact role and mechanism of cathepsin D in glioma invasion.
Paper-Based Radial Chromatographic Immunoassay for the Detection of Pathogenic Bacteria in Milk
Luo, Ke,Ryu, Jian,Seol, In-Hye,Jeong, Ki-Baek,You, Sang-Mook,Kim, Young-Rok American Chemical Society 2019 ACS APPLIED MATERIALS & INTERFACES Vol.11 No.50
<P>Here, a paper-based radial flow chromatographic immunoassay (RFCI) employing gold nanoparticles (AuNPs) as chromatic agents was developed for the detection of <I>Escherichia coli</I> O157:H7 in whole milk. A 4-repeated gold-binding peptide-tagged (4GBP) streptococcal protein G (SPG) fusion protein was constructed as a bifunctional linker to immobilize antibodies on the surface of AuNPs with a well-oriented form based on the specific affinity of GBP and SPG to the gold and Fc portion of the antibody, respectively. 4GS@AuNPs prepared with the bifunctional linker protein exhibited excellent colloidal stability even at high salt concentrations of up to 500 mM, which is a critical requirement for its application to a broad range of biological and food samples. The enhanced colloidal stability and excellent binding capability of the immuno-4GS@AuNPs toward target bacteria lowered the detection limit of RFCI for target pathogenic bacteria in whole milk as low as 10<SUP>3</SUP> CFU/mL, which is by an order of magnitude lower than that of conventional immuno-AuNPs prepared with physical adsorption of antibodies. The RFCI pattern could also be converted into a grayscale value by simple image processing for quantitative determination of target pathogenic bacteria. This paper-based detection system would provide an effective means of monitoring the presence of food-borne pathogens in real food samples with naked eyes.</P> [FIG OMISSION]</BR>
이양현,류지안,김휘중,이상희 대한생물치료정신의학회 2003 생물치료정신의학 Vol.9 No.2
Objectives : Delirium s a highly prevalent disease that occurs in about 1 5 2 0 % of all general admissions to hospital but there is no standardized rating scale that identifies and assesses the symptom severity for delirium in Korea. The Deliruim Rating Scale (DRS) is a widely used delirium rating instrument that specifically, sensitively, and reliably measures delirium symptoms. This study addresses the reliability and validity of Korean version of Delirium Rating Scale(K-DRS) in Korea. Methods : 28 patients with delirium, 28 patients with dementia, 27 patients with schizophrenia, and 21 patients with other mental disorder by DSM-IV entered this study All patients received a structured psychopathology assessment including K-DRS Korean version of Mini-Mental Status Examination (MMSE-K) Clinical Global Impression scale(CG1) and in addition schizophrenia group had done Brief Psychiatric Rating Scale (BPRSI Each instrument scores were compared among the four diagnostic groups by one-way analysis of variance with post hoc comparisons to determine where the difference lie K-DRS scores were compared with 'after usual treatment' scores in a subset of delirious subjects Cutoff scores for K-DRS were determined by using receiver-operator Characteristic (ROC) analyses to determine acceptable levels of sensitivity and specificity Inter-subtest Pearson's correlations, Cronbach's alpha coefficient to assess internal consistency and inter-rater reliability were used for the assessment of reliability of K-DRS. Results : The mean K-DRS score was significantly higher in the delirium group compared with each of the other groups, But mean MMSE-K score was not significantly different between delirium and dementia group Area under curve of K-DRS was 0 998K E=0.002) and its optimal cutoff point for delirium was estimated as 16 5 KDRS score after usual treatment improved from a mean(S.D) of 23 5(3.2) to 3.1(1.7) indicating an ability to measure the severity of delirium K-DRS was found to have significantly high internal consistency(Cronbach's alpha coefficient=0.88) inter-rater reliability (r=0.98 p<0.0001) Conclusion : We confirmed that K-DRS is a reliable valid, and useful diagnostic instrument for delirium.