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Study on Flexural and Shear Behavior of UHPC Rhombus-Strip-Shaped Joint
Shuwen Deng,Banfu Yan,Lian Shen,Mingxin Qin,Yina Jia 한국콘크리트학회 2024 International Journal of Concrete Structures and M Vol.18 No.1
Accelerated bridge construction (ABC) has many advantages for bridge construction in modern society. While for ABC, the post-cast joint is always the weakest and most critical part. This paper presents a UHPC rhombus-strip-shaped (RSS) joint suitable for ABC. Several model tests were carried out to verify its resistance to flexural and shear. First, large-scale model tests are advanced to confirm its flexural properties. The results show that densified and welded joint interface rebars can significantly improve the ultimate bearing capacity and durability-based cracking stress of the RSS joint beams, and the ultimate bearing capacity can reach 90% of the complete beam. Then the shear-resistance tests were carried out. The results show that the UHPC RSS joint beam has excellent bending-shear mechanical properties and better ductility. Lastly, the ultimate flexural bearing capacity and shear-resistance capacity calculation methods were obtained.
Ke-Tao Du,Jia-Qin Deng,Xu-Guang He,Zhao-ping Liu,Cheng Peng,Mingsheng Zhang 한국조직공학과 재생의학회 2018 조직공학과 재생의학 Vol.15 No.3
miR-214 plays a major role in the self-renewal of skin tissue. However, whether miR-214 regulates the proliferation and differentiation of human hair follicle stem cells (HFSCs) is unknown. Primary HFSCs were isolated from human scalp skin tissue, cultured, and identified using flow cytometry. An miR-214 mimic and inhibitor were constructed for transfection into HFSCs. The MTS and colony formation assays examined cell proliferation. Immunofluorescence detected the localization and expression levels of TCF4, b-catenin, and differentiation markers. Luciferase reporter and TOP/FOP Flash assays investigated whether miR-214 targeted EZH2 and regulated the Wnt/b-catenin signaling pathway. Western blot determined the expression levels of enhancer of zeste homolog 2 (EZH2), Wnt/b-catenin signaling-related proteins, and HFSC differentiation markers in cells subjected to miR-214 transfection. miR-214 expression was remarkably decreased during the proliferation and differentiation of HFSCs into transit-amplifying (TA) cells. Downregulation of miR- 214 promotes the proliferation and differentiation of HFSCs. Overexpression of miR-214 led to decreased expression of EZH2, b-catenin, and TCF-4, whereas downregulation of miR-214 resulted in increased expression of EZH2, b-catenin, and TCF-4 as well as TA differentiation markers. Immunofluorescence assay revealed that inhibiting miR-214 triggered the entry of b-catenin and TCF-4 into the nucleus. The luciferase reporter and TOP/FOP Flash assays demonstrated that miR- 214 directly targets EZH2 and affects Wnt/b-catenin signaling. The miR-214/EZH2/b-catenin axis could be considered a candidate target in tissue engineering and regenerative medicine for HFSCs.
Genetic Epidemiological Analysis of Esophageal Cancer in High-incidence Areas of China
Wang, Kai-Juan,Yang, Jun-Xia,Shi, Jia-Chen,Deng, Song-Yuan,Cao, Xiao-Qin,Song, Chun-Hua,Wang, Peng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22
Genetic epidemiological studies have shown that genetic susceptibility to esophageal cancer (EC) is an important cause of its high incidence within families in some areas of China. The purpose of this study was to obtain evidence of a genetic basis of EC in Xin-an and Xin-xiang counties in China. Familial aggregation and complex segregation analyses were performed of 79 EC families in these counties. The heritability of EC was examined using Falconer's method and complex segregation analysis was conducted with the SEGREG program in Statistical Analysis for Genetic Epidemiology (SAGE version 5.3.1). The results showed that the distribution of EC in families did not fit well into a binomial distribution. The heritability of EC among first-degree and second-degree relatives was $67.0{\pm}7.31%$ and $43.1%{\pm}9.80%$, respectively, and the summing up powered heritability was $53.2{\pm}6.74%$. The segregation ratio was 0.045. Complex segregation analysis showed that the genetic model of EC was additive. The current results provide evidence for an inherited propensity to EC in certain high-risk groups in China, and support efforts to identify the genes that confer susceptibility to this disease.