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[PA-0008] Assembly and Analysis of the Complete mitogenome of Silybum marianum
Jeongwoo Lee(Jeongwoo Lee),Yedomon Ange Bovys Zoclanclounon(Yedomon Ange Bovys Zoclanclounon ),Hwajin Jung(Hwajin Jung),Taeho Lee(Taeho Lee),Jeonggu Kim(Jeonggu Kim),Guhwang Park(Guhwang Park),Keunpyo 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-
Park, Chang-Deuk,Kwon, Kwanik,Yoo, Nakyung,Lee, Jung-Hyun,Kang, Dong-Won,Park, Jong-Sung,Yoo, Jeongwoo,Kim, Keun-Sik,Yoon, Ju-Duk National Institute of Ecology 2021 국립생태원회보(PNIE) Vol.2 No.2
To restore the Gold-spotted pond frog (Pelophylax chosenicus), a Korean endangered frog, 600 captive-reared individuals were reintroduced between August and September 2019 into an aquatic garden in the National Institute of Ecology where P. chosenicus had previously inhabited. After reintroduction, six post-release monitoring sessions were conducted from August 2019 to May 2020. Monitoring was performed using three methods (counting calls, observation, and capture) from sunset to midnight near release sites, eliminating potential threat factors. Snout-vent lengths and body weights of recaptured individuals were measured before they were released immediately. We noted that both snout-vent lengths and body weights of recaptured individuals were slightly higher than those before. The average recapture rate was 4.66%. Reintroduced frogs were recaptured at the last monitoring session conducted in May 2020, indicating that these reintroduced frogs hibernated during the winter successfully. We found that these reintroduced frogs successfully settled after release. This result will be useful for establishing management strategies for endangered frogs in Korea. Particularly, post-release monitoring could be an essential approach in the restoration program of a target species.
Production of <small>D</small>-ribose by metabolically engineered <i>Escherichia coli</i>
Park, Hae-Chul,Kim, Yun-Jung,Lee, Chang-Wan,Rho, Yong-Taek,Kang, JeongWoo,Lee, Dae-Hee,Seong, Yeong-Je,Park, Yong-Cheol,Lee, Daesang,Kim, Sung-Gun Elsevier 2017 PROCESS BIOCHEMISTRY Vol.52 No.-
<P><B>Abstract</B></P> <P> <I>Escherichia coli</I> was metabolically engineered for the production of <SMALL>D</SMALL>-ribose, a functional five-carbon sugar, from xylose. For the accumulation of <SMALL>D</SMALL>-ribose, two genes of transketolase catalyzing the conversion of <SMALL>D</SMALL>-ribose-5-phosphate to sedoheptulose-7-phosphate in pentose phosphate pathway were disrupted to create a transketolase-deficient <I>E. coli</I> SGK013. In batch fermentation, <I>E. coli</I> SGK013 grew by utilizing glucose and then started to produce <SMALL>D</SMALL>-ribose from xylose after glucose depletion. <I>E. coli</I> SGK013 produced 0.75g/L of <SMALL>D</SMALL>-ribose, which was identical to the standard <SMALL>D</SMALL>-ribose as confirmed by HPLC and LC/MS analyses. To improve D-ribose production, the <I>ptsG</I> gene encoding the glucose-specific IICB component was disrupted additionally, resulting in the construction of <I>E. coli</I> SGK015. The carbon catabolite repression-negative <I>E. coli</I> SGK015 utilized xylose and glucose simultaneously and produced up to 3.75g/L of <SMALL>D</SMALL>-ribose, which is a 5-fold improvement compared to that of <I>E. coli</I> SGK013.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Transketolase-deficient <I>E. coli</I> was constructed to produce <SMALL>D</SMALL>-ribose from xylose. </LI> <LI> <SMALL>D</SMALL>-Ribose produced in the <I>E. coli</I> was identical to standard <SMALL>D</SMALL>-ribose in HPLC and LC/MS analyses. </LI> <LI> The transketolase-deficient <I>E. coli</I> was further engineered to uptake glucose and xylose simultaneously for facilitating <SMALL>D</SMALL>-ribose production. </LI> <LI> The simultaneous uptake of glucose and xylose in the <I>E. coli</I> resulted in a 5-fold improvement of <SMALL>D</SMALL>-ribose production. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
A low-power MDDI-client architecture using on-off byte counter
Jeongwoo Park,Suki Kim,Kwang-Hyun Baek IEEE 2010 IEEE transactions on consumer electronics Vol.56 No.3
<P>This paper presents a Mobile Display Digital Interface (MDDI)-client architecture which is suitable for low-power, small-area, and low-cost mobile applications. Compared to the power dissipation of an MDDI client with a byte counter that has continuous transitions for all packet data, the proposed MDDI client reduces power consumption in state transitions up to 50% by using robust Finite State Machine (FSM) and on-off byte counter. The proposed scheme detects invalid data in packets and turns off the operation of the byte counter to minimize client's power dissipation. The MDDI-client architecture is implemented and verified on FPGA modules.</P>
Park, Jin Han,Hwang, Jongchul,Kim, Juhyung,Park, Byeongmuk,Park, Juyoung,Son, Jeongwoo,Lee, Sihun Eudoxus Press LLC 2016 Journal of computational analysis and applications Vol.20 No.5
<P>This paper presents new methods for measuring similarity between generalized intuitionistic fuzzy sets (GIFSs) and its application to pattern recognition. Firstly, the geometrical interpretation of GIFSs is carefully reviewed and then the results of the interpretation is utilized to generate new methods for measuring similarity in order to calculate the degree of similarity between GIFSs. Numerical example is given to illustrate the application of the proposed similarity measures. Finally, we also use the proposed similarity measures to characterize the similarity between linguistic-variables.</P>
IMA: Identifying disease-related genes using MeSH terms and association rules
Kim, Jeongwoo,Bang, Changbae,Hwang, Hyeonseo,Kim, Doyoung,Park, Chihyun,Park, Sanghyun Elsevier 2017 Journal of biomedical informatics Vol.76 No.-
<P><B>Abstract</B></P> <P>Genes play an important role in several diseases. Hence, in biology, identifying relationships between diseases and genes is important for the analysis of diseases, because mutated or dysregulated genes play an important role in pathogenesis. Here, we propose a method to identify disease-related genes using MeSH terms and association rules. We identified genes by analyzing the MeSH terms and extracted information on gene-gene interactions based on association rules. By integrating the extracted interactions, we constructed gene-gene networks and identified disease-related genes. We applied the proposed method to study five cancers, including prostate, lung, breast, stomach, and colorectal cancer, and demonstrated that the proposed method is more useful for identifying disease-related and candidate disease-related genes than previously published methods. In this study, we identified 20 genes for each disease. Among them, we presented 34 important candidate genes with evidence that supports the relationship of the candidate genes with diseases.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We propose a method to identify disease-related genes using MeSH terms and association rules. </LI> <LI> We construct gene-gene interaction networks for each disease. </LI> <LI> We identify disease-related genes and meaningful disease-related candidate genes. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
고교학점제 도입을 위한 일반고 교·강사 제도의 운영 실태 분석
박수정(Park, SooJung),방효비(Fang, XiaoFei),박정우(Park, JeongWoo),고경희(Ko, GyungHui) 학습자중심교과교육학회 2020 학습자중심교과교육연구 Vol.20 No.8
고교학점제 도입에 있어서 교·강사 관련 제도가 중요하다는 문제의식에 따라, 고등학교 현장에서 운영되고 있는 교·강사 제도의 운영 실태를 구체적으로 분석하였다. 이를 위하여 2019학년도 일반고등학교 전체(정보공시)와 고교학점제 연구·선도학교(설문조사)의 교·강사 제도 운영 실태를 분석하였다. 주요 연구결과는 다음과 같다. 첫째, 일반고 교사의 평균 수업시수는 창의적체험활동 포함시 평균 16.9시간, 제외시 평균 15.1시간이고, 일반고 교사의 52%가 1개 과목, 38%가 2개 과목을 담당하며, 88%의 교사가 1개 자격을 소지하는 것으로 나타났다. 향후 다과목 지도에 대한 부담 완화와 지도 역량 강화가 필요하다. 둘째, 순회교사의 활용은 평균 5시간 정도로, 좀 더 적극적인 활용과 제도적 토대 마련이 필요하다. 셋째, 일반고의 정규수업을 담당하는 강사의 비율은 1%로 현재 미미한 수준이나, 향후 다양한 선택과목이 개설될 경우 강사의 확보와 질 관리, 강사료 현실화가 필요하다. 넷째, 다과목을 지도하는 교사와 순회교사에 대한 인센티브로 현재 가장 우세한 성과급 산정 시 가산점 외에, 교직 문화의 인식 전환과 총체적인 지원 방안에 대한 논의가 필요하다. 다섯째, 선택과목 확대로 인한 교과목과 수업학급수 증가는 수업시수, 학급당 학생수, 담임제도 등에 대한 기존 개념의 변화를 요구한다. In accordance with the awareness that the system related to teachers and instructors is important in the introduction of the high school credit system. For this purpose, the actual conditions of all the general high schools(Korean Education Statistics Service, KESS) in 2019 and Research·Initiative schools(Survey) were analyzed. The main findings are as follows. First, the average regular teaching time in high schools including Creative Experience Activities is 16.9 hours, except for 15.1 hours. 52% of teachers in high schools teach one subject, 38% of teachers teach two subjects, 88% of teacher have one teacher certification. The teachers who teach multiple subjects should ease the burden and strengthen the guidance. Second, the average teaching time of the itinerant teacher was five hours, require more active use of teacher and need to establish institutional foundation. Third, only 1% of instructors in general high schools regular classes, which is currently low, if various elective subjects are offered in the future, the number and quality of instructors need to increased, and realize lecturer pay. Fourth, the itinerant teacher and the teacher who teach multiple subjects, in addition to the most advantageous bonus of performance-based pay as an incentive to give, also need to discuss the change and support of teaching culture. Fifth, the number of course and classes increased due to the increase of elective course that requires a change in the existing concepts of teaching time, class size studies, homeroom system.
Kang, JeongWoo,Park, Su-Jeong,Park, Hae-Chul,Gedi, Vinayakumar,So, ByungJae,Lee, Kwang-Jick Humana Press 2014 Applied biochemistry and biotechnology Vol.174 No.1
<P>Nonsteroidal anti-inflammatory drugs (NSAIDs) are the group of drugs having the therapeutic efficacy of analgesic and antipyretic. To detect health-threatening residues of NSAIDs, a fast and easy multiresidue method based on liquid chromatography tandem mass spectrometry (LC-MS/MS) was described. Ten NSAIDs were extracted from the tissues using 2 mL of acetonitrile and 0.1 mL of 2 mM ammonium formate in distilled water. After clean-up using C18 sorbent, it was evaporated under nitrogen, reconstituted with 1 mL distilled water and analyzed by LC-MS/MS. The method was validated based on guideline for residue testing laboratory. Furthermore, the method has also been applied successfully to detect ten NSAIDs from bovine, porcine, and chicken liver tissues. In a total of 315 liver samples tested, acetylic salicylic acid was detected from 28 porcine and 2 chicken liver tissues at levels of 13 576 and 50 53 ng/g, respectively. Subsequently, paracetamol was detected in 15 porcine liver tissues with a detection levels of 28 381 ng/g. Phenylbutazone and its metabolite, oxyphenylbutazone, were detected at 247 and 15 ng/g range in one of the bovine liver tissue, respectively.</P>