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Optical investigation on zinc doped cadmium sulphide nanocrystalline thin films
J.R. Jayaramaiah,R. Shamanth,V. Jayanth,K.S. Shamala 한국물리학회 2016 Current Applied Physics Vol.16 No.7
Optical investigation on blue shift behavior of zinc doped cadmium sulphide nano-crystalline thin films have been prepared by spray pyrolysis method at 375 ± 10 C. The crystallinity and phase have been characterized by glancing angle X-ray diffraction. The XRD peaks confirm the hexagonal structure of cadmium sulphide. The crystallites sizes are found its range of 15e20 nm. The surface morphology is analyzed by using field emission scanning electron microscopy. The morphology of the film is seen as uniform distribution of homogeneous fine solid grains which are compact in nature. Optical absorption spectrum reveals an absorption peak at 475 nm. Indicating that blue shift due to quantum confinement effect, as a result the direct energy gap is increased and found its value is 2.91 eV. Raman spectrum reveals the longitudinal optical phonon peaks are at 302 cm1 and 603 cm1. The noticeable asymmetry and frequency shift confirm the decrease in particle size. X-ray photoelectron spectroscopy reveals the surface composition and binding energy of elements and it confirm the presence of zinc. The photoluminescence spectrum reveals an emission peak at 728 nm is analyzed. Zn doped cadmium sulphide thin film is useful for window material in solar cells and luminescent red phosphor.
Lee, So Eui,Gupta, Ravi,Jayaramaiah, Ramesha H.,Lee, Seo Hyun,Wang, Yiming,Park, Sang-Ryeol,Kim, Sun Tae The Korean Society of Plant Pathology 2017 Plant Pathology Journal Vol.33 No.5
Xanthomonas oryzae pv. oryzae (Xoo), the causative agent of bacterial blight, is a major threat to rice productivity. Here, we performed RNA-Seq based transcriptomic analysis of Xoo transcripts isolated under in planta growth (on both susceptible and resistant hosts) and in vitro culture conditions. Our in planta extraction method resulted in successful enrichment of Xoo cells and provided RNA samples of high quality. A total of 4,619 differentially expressed genes were identified between in planta and in vitro growth conditions. The majority of the differentially expressed genes identified under in planta growth conditions were related to the nutrient transport, protease activity, stress tolerance, and pathogenicity. Among them, over 1,300 differentially expressed genes were determined to be secretory, including 184 putative type III effectors that may be involved in Xoo pathogenicity. Expression pattern of some of these identified genes were further validated by semi-quantitative RT-PCR. Taken together, these results provide a transcriptome overview of Xoo under in planta and in vitro growth conditions with a focus on its pathogenic processes, deepening our understanding of the behavior and pathogenicity of Xoo.
Evaluation of Bioactive Compounds in Strawberry Fruits by a Targeted Metabolomic Approach
Min Jung Ko,Ramesha H Jayaramaiah,Ravi Gupta,So Wun Kim,Jae Uk An,Ziyu Wang,Mae Li,Nam Jun Kang,Kwang-Pyo Hong,Jum-Soon Kang,Sun Tae Kim,Young Whan Choi 한국원예학회 2017 원예과학기술지 Vol.35 No.6
Strawberry (Fragaria ×ananassa Duch.) is referred to as a functional fruit because of its high nutritional value. Here, we used a targeted metabolomics approach to investigate the quantitative variation in strawberry bioactive compound concentration across cultivars and breeding lines and during fruit ripening. We also compared the efficiency of 95% ethyl alcohol (EtOH), 50% EtOH, and water in extracting these compounds. High-performance liquid chromatography (HPLC) and multivariate analysis revealed that 50% EtOH extracted bioactive compounds more efficiently than 95% EtOH and water. Screening of targeted bioactive compounds extracted with 50% EtOH in six cultivars and 28 breeding lines revealed high abundance of anthocyanins, flavonols, flavanols, and cinnamic acid, which constituted up to 6.4 mg·g<SUP>-1</SUP> of fresh weight of fruits. The highest concentration of phenolic compounds was observed in breeding lines ‘SB08’, ‘SB11’, ‘Arang’, ‘SB09’, and ‘SB10’. Furthermore, significant changes in the concentration of these compounds occurred during fruit ripening in four strawberry cultivars. Overall, our results highlight the chemotypic relationship between cultivars, breeding lines, and fruit ripening associated-changes in strawberries, and these findings provide a chemometric method for choosing optimal cultivars/breeding lines.
이소의,Ravi Gupta,Ramesha H. Jayaramaiah,이서현,Yiming Wang,박상렬,김선태 한국식물병리학회 2017 Plant Pathology Journal Vol.33 No.5
Xanthomonas oryzae pv. oryzae (Xoo), the causative agent of bacterial blight, is a major threat to rice productivity. Here, we performed RNA-Seq based transcriptomic analysis of Xoo transcripts isolated under in planta growth (on both susceptible and resistant hosts) and in vitro culture conditions. Our in planta extraction method resulted in successful enrichment of Xoo cells and provided RNA samples of high quality. A total of 4,619 differentially expressed genes were identified between in planta and in vitro growth conditions. The majority of the differentially expressed genes identified under in planta growth conditions were related to the nutrient transport, protease activity, stress tolerance, and pathogenicity. Among them, over 1,300 differentially expressed genes were determined to be secretory, including 184 putative type III effectors that may be involved in Xoo pathogenicity. Expression pattern of some of these identified genes were further validated by semi-quantitative RT-PCR. Taken together, these results provide a transcriptome overview of Xoo under in planta and in vitro growth conditions with a focus on its pathogenic processes, deepening our understanding of the behavior and pathogenicity of Xoo.