Pseudo-renal Failure Caused by Urinary Bladder Rupture in Multiple Trauma Patient

( Jihoon Jang ),( Kyoung Hoon Lim ) 대한외상학회 2016 大韓外傷學會誌 Vol.29 No.4

Pseudo-renal failure presents with renal failure characteristics, such as hypercreatininemia and hyperkalemia without a change in glomerular filtration rate or structure of the kidney. Pseudo-renal failure due to trauma is difficult to diagnose, because symptoms are non-specific and other factors may cause hypercreatininemia and hyperkalemia. In a trauma patient, especially one with pelvic injury, the abrupt elevation of potassium, blood urea nitrogen, and creatinine levels without previous medical history is a key feature in the diagnosis of urinary ascites. We report a case of pseudo-renal failure caused by intraperitoneal bladder rupture in a multiple trauma patient. [ J Trauma Inj 2016; 29: 191-194 ]

Oxidized DJ-1 Levels in Urine Samples as a Putative Biomarker for Parkinson's Disease

Jang, Jihoon,Jeong, Soyeon,Lee, Sung Ik,Seol, Wongi,Seo, Hyemyung,Son, Ilhong,Ho, Dong Hwan Hindawi 2018 Parkinson’s disease Vol.2018 No.-

<P>Parkinson's disease (PD) is the second most common neurodegenerative disease. Oxidative stress is the most critical risk factor for neurodegenerative diseases, including Alzheimer's disease (AD) and Huntington's disease (HD). Numerous reports have demonstrated that oxidative stress aggravates cytotoxicity in dopaminergic neurons and accelerates the formation of protein inclusions. In addition, oxidative stress, such as 4-hydroxynonenal (HNE), oxidized protein, and dopamine quinone, are related to PD progression. <I>DJ-1</I> is a PD-causative gene, and it plays a pivotal role as a sensor and eliminator of oxidative stress. Several studies have shown that oxidized DJ-1 (OxiDJ-1) formation is induced by oxidative stress. Hence, previous studies suggest that oxidized DJ-1 could be a biomarker for PD. We previously reported higher DJ-1 levels in Korean male PD patient urine exosomes than male non-PD controls. We speculate that OxiDJ-1 levels in PD patient urine might be higher than that in non-PD controls. In this study, we established an ELISA for OxiDJ-1 using recombinant DJ-1 treated with H<SUB>2</SUB>O<SUB>2</SUB>. Using Western blot assay and ELISA, we confirmed an increase of OxiDJ-1 from HEK293T cells treated with H<SUB>2</SUB>O<SUB>2</SUB>. Using our ELISA, we observed significantly higher, 2-fold, OxiDJ-1 levels in the urine of Korean PD patients than in non-PD controls.</P>

Organic–inorganic vesicular hybrids driven by assembly of dendritic amphiphiles: site-selective encapsulation of nanoparticles

Jang, Jihoon,Kim, Jung-Keun,Choi, Jin-Woo,Hwang, Taek-Sung,Jo, Mijung,Kim, Inhye,Cho, Byoung-Ki,Lee, Eunji The Royal Society of Chemistry 2013 Chemical communications Vol.49 No.73

<P>The amphiphilic block codendrimer self-assembled into supramolecular vesicles with a bilayer membrane in both hydrophilic and hydrophobic solvents. Surface-modified quantum dots (QDs) were successfully entrapped within the wall of vesicles through a simple self-assembly process without any structural disruption.</P> <P>Graphic Abstract</P><P>A Janus amphiphilic codendrimer can lead to the formation of organic–inorganic hybrid hollow capsules by co-assembly with surface-modified quantum dots in both hydrophilic and hydrophobic solvents. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c3cc42213e'> </P>

Forearm Replantation for a Patient Presented with Major Amputation Injury: A Case Report

( Jihoon Jang ),( Kyoung Hoon Lim ),( Joon-woo Kim ),( Hyung-kee Kim ) 대한외상학회 2016 大韓外傷學會誌 Vol.29 No.4

With the development of safety measures for employees who work with dangerous machinery, the frequency of amputation injuries has been decreasing with resultant decrease in replantation procedures. However, in some patients with major amputation injury, replantation is still necessary for the preservation of limb and it s function. The replantation of the upper extremity (UE) is a complex and technically demanding surgical procedure. For the successful replantation of UE, the type of injury, reconstruction sequence, ischemic time, and other combined injury of patient should be considered. We report a case of major amputation of UE by guillotine-type injury and discuss the treatment process of this patient. [ J Trauma Inj 2016; 29: 187-190 ]

A SCAN Chain with Rotator Layout Generator

Jihoon Jang,Jaeduk Han 대한전자공학회 2023 대한전자공학회 학술대회 Vol.2023 No.6

Increase in anti-apoptotic molecules, nucleolin, and heat shock protein 70, against upregulated LRRK2 kinase activity

Jang, Jihoon,Oh, Hakjin,Nam, Daleum,Seol, Wongi,Seo, Mi Kyoung,Park, Sung Woo,Kim, Hyung Gun,Seo, Hyemyung,Son, Ilhong,Ho, Dong Hwan ZOOLOGICAL SOCIETY OF KOREA 2018 ANIMAL CELLS AND SYSTEMS Vol.22 No.5

<P><B>ABSTRACT</B></P><P>Leucine-rich repeat kinase 2 (LRRK2) is involved in Parkinson’s disease (PD) pathology. A previous study showed that rotenone treatment induced apoptosis, mitochondrial damage, and nucleolar disruption via up-regulated LRRK2 kinase activity, and these effects were rescued by an LRRK2 kinase inhibitor. Heat-shock protein 70 (Hsp70) is an anti-oxidative stress chaperone, and overexpression of Hsp70 enhanced tolerance to rotenone. Nucleolin (NCL) is a component of the nucleolus; overexpression of NCL reduced cellular vulnerability to rotenone. Thus, we hypothesized that rotenone-induced LRRK2 activity would promote changes in neuronal Hsp70 and NCL expressions. Moreover, LRRK2 G2019S, the most prevalent LRRK2 pathogenic mutant with increased kinase activity, could induce changes in Hsp70 and NCL expression. Rotenone treatment of differentiated SH-SY5Y (dSY5Y) cells increased LRKK2 levels and kinase activity, including phospho-S935-LRRK2, phospho-S1292-LRRK2, and the phospho-moesin/moesin ratio, in a dose-dependent manner. Neuronal toxicity and the elevation of cleaved poly (ADP-ribose) polymerase, NCL, and Hsp70 were increased by rotenone. To validate the induction of NCL and Hsp70 expression in response to rotenone, cycloheximide (CHX), a protein synthesis blocker, was administered with rotenone. Post-rotenone increased NCL and Hsp70 expression was repressed by CHX; whereas, rotenone-induced kinase activity and apoptotic toxicity remained unchanged. Transient expression of G2019S in dSY5Y increased the NCL and Hsp70 levels, while administration of a kinase inhibitor diminished these changes. Similar results were observed in rat primary neurons after rotenone treatment or G2019S transfection. Brains from G2019S-transgenic mice also showed increased NCL and Hsp70 levels. Accordingly, LRRK2 kinase inhibition might prevent oxidative stress-mediated PD progression.</P><P><B>Abbreviations:</B> 6-OHDA: 6-hydroxydopamine; CHX: cycloheximide; dSY5Y: differentiated SH-SY5Y; g2019S tg: g2019S transgenic mouse; GSK/A-KI: GSK2578215A kinase inhibitor; HSP70: heat shock protein 70; LDH: lactose dehydrogenase; LRRK2: leucine rich-repeat kinase 2; MPTP: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine; myc-GS LRRK2: myc-tagged g2019S LRRK2; NCL: nucleolin; PARP: poly(ADP-ribose) polymerase; PD: Parkinson’s disease; PINK1: PTEN-induced putative kinase 1; pmoesin: phosphorylated moesin at t558; ROS: reactive oxygen species</P>

All-solution-processed PbS quantum dot solar modules

Jang, Jihoon,Shim, Hyung Cheoul,Ju, Yeonkyeong,Song, Jung Hoon,An, Hyejin,Yu, Jong-Su,Kwak, Sun-Woo,Lee, Taik-Min,Kim, Inyoung,Jeong, Sohee The Royal Society of Chemistry 2015 Nanoscale Vol.7 No.19

이형질의 다중 에이전트 환경에서의 에이전트 동적 구동 기법

장지훈(Jihoon Jang),강기영(Kiyoung Kang),최중민(Joongmin Choi) 한국정보과학회 1998 한국정보과학회 학술발표논문집 Vol.25 No.2Ⅱ

최근 다중 에이전트에 대한 연구가 활발히 진행되고 있다. 특히 이질적이고 분산된 컴퓨팅 환경에 효율적이며 적응성이 향상된 여러 가지 형태의 다중 에이전트가 개발되고 있다. 하지만 아직 이질적인 에이전트의 동적구동을 통한 효율 향상과 유연성에 대한 연구는 초보단계이다. 본 논문에서는 자바로 된 다중 에이전트 기반 구조에서 서로 각기 다른 컴퓨터 언어로 만들어진 에이전트가 분산된 환경에서 상호 협력을 통해 작업을 수행하기 위해서 사용자나 다른 에이전트의 요청으로 동적으로 수행될 수 있는 기술을 설계하고 구현하였다. 또한 서로 다른 에이전트간의 협력을 가능하게 하는 통신기능을 다중 에이전트 기반기술에 적용함으로써 사용자의 요구에 더욱더 능동적으로 대처하고, 다양한 환경 변화에 강한 적응성과 개선된 협동능력을 제공하도록 구현하였다.

발전소 옥외저탄장 자연발화에 대한 수치 해석적 연구

장지훈(Jihoon Jang),박석운(Seok-un Park),신동익(Dong-ik Shin),김재관(Jae-kwan Kim) 한국화재소방학회 2022 한국화재소방학회 학술대회 논문집 Vol.2022 No.추계

국내 석탄화력 발전소는 역청탄과 함께 고 수분 및 고 휘발분을 포함하는 저등급 아역청탄을 혼소하는 방법으로 운영하고 있다. 아역청탄은 저탄장에 하역 후 1~3개월을 보관하여 사용하고 있으며, 발전소 1개 본부의 저탄현황 조사결과, 보관된 전체 탄 중 50% 이상의 탄종에서 자연발화가 발생하고 있음을 확인하였다. 석탄의 자연발화로 인해 저탄장 주변 대기로 유해가스(CO, HC, 합성가스 등)를 배출하고, 연소로 인해 연료의 고유열량이 손실(~5.85%) 되기 때문에 이를 방지하기 위한 체계적인 관리가 필요하다. 본 연구에서는 석탄의 자연발화에 영향을 끼치는 주요 변수를 분석하여 상관관계를 도출하였으며 이를 지배방정식에 적용하여 전산해석을 위한 모델링을 수행하였다. 저탄의 물리적 특성 및 탄 성상에 따른 화학적 특성, 외부변수 등을 전산해석의 초기/경계 조건으로 설정하여 실제 발전소 저탄장의 운영조건에서의 자연발화 예측 및 현장 계측 데이터를 통한 검증을 수행하였다. 저탄장에서의 자연발화 현상은 석탄과 산소의 화학반응으로 방출되는 열과 수분에 의한 습윤열, 태양으로부터 도달하는 복사열 등이 저탄파일 내부에 축적되어 발생한다. 주요 인자는 저탄장의 수분 및 바람, 석탄의 입도 및 공극률, 저탄높이, 저탄각 등으로, 개발한 예측모델에는 날씨에 따른 대류열전달 및 산화제의 저탄파일 외부⋅내부에서의 유동특성을 모사하기 위해 난류(Turbulence), 에너지(Enthalpy) 방정식, 수분에 따른 효과 및 석탄의 화학반응을 고려하기 위해 화학종 전달(Species transport) 방정식을 적용하였으며, 석탄의 성상, 입자크기, 공극률 등은 저탄 파일(Pile) 내부를 다공성 매질(Porous material)로 모델링하여 물리적 특성을 반영하였다. 저탄 파일의 형상(높이, 형태, 2/3차원), 대기온도(계절, 일교차), 풍속, 상대습도, 탄종별 특성(Specific heat, Conductivity, Diameter, Porosity)에 대한 Parametric Study를 통해 화학 반응률(Reaction rate)에 대한 영향성을 평가하고, 각 지배방정식의 Correlation 및 Coefficients를 도출하였다. 도출된 모델을 활용한 입고탄의 저장기간(~120일), 저탄장 운영환경에서의 자연발화 전산해석을 수행하였으며, 예측결과를 현장계측 데이터와 비교하였다. 그 결과, 자연발화 취약 위치(Pile 경계면 하부 1/3 지점) 예측결과 및 취약 위치에서의 승온률(예측:0.123℃ /hr, 실제:0.129 ℃/hr) 비교에서 96% 이상의 정성적⋅정량적 예측 정확도를 보이는 것으로 검증되었다. 대상 발전소에서는 본 연구를 통해 도출된 자연발화 예측모델을 호기별 혼탄 계획 수립 및 입고탄 관리시스템에 적용⋅활용 할 계획이다.

Synergistic toxicity with copper contributes to NAT2-associated isoniazid toxicity

Yoon Jihoon G.,Jang Dong Geon,Cho Sung-Gyu,Lee Chaeyoung,Noh Shin Hye,Seo Soo Kyung,Yu Jung Woo,Chung Hyeon Woo,Han KyeoRe,Kwon Soon Sung,Han Dai Hoon,Oh Jaeseong,Jang In-Jin,Kim Sang-Hoon,Jee Young-K 생화학분자생물학회 2024 Experimental and molecular medicine Vol.56 No.-

Anti-tuberculosis (AT) medications, including isoniazid (INH), can cause drug-induced liver injury (DILI), but the underlying mechanism remains unclear. In this study, we aimed to identify genetic factors that may increase the susceptibility of individuals to AT-DILI and to examine genetic interactions that may lead to isoniazid (INH)-induced hepatotoxicity. We performed a targeted sequencing analysis of 380 pharmacogenes in a discovery cohort of 112 patients (35 AT-DILI patients and 77 controls) receiving AT treatment for active tuberculosis. Pharmacogenome-wide association analysis was also conducted using 1048 population controls (Korea1K). NAT2 and ATP7B genotypes were analyzed in a replication cohort of 165 patients (37 AT-DILI patients and 128 controls) to validate the effects of both risk genotypes. NAT2 ultraslow acetylators (UAs) were found to have a greater risk of AT-DILI than other genotypes (odds ratio [OR] 5.6 [95% confidence interval; 2.5–13.2], P = 7.2 × 10−6). The presence of ATP7B gene 832R/R homozygosity (rs1061472) was found to co-occur with NAT2 UA in AT-DILI patients (P = 0.017) and to amplify the risk in NAT2 UA (OR 32.5 [4.5–1423], P = 7.5 × 10−6). In vitro experiments using human liver-derived cell lines (HepG2 and SNU387 cells) revealed toxic synergism between INH and Cu, which were strongly augmented in cells with defective NAT2 and ATP7B activity, leading to increased mitochondrial reactive oxygen species generation, mitochondrial dysfunction, DNA damage, and apoptosis. These findings link the co-occurrence of ATP7B and NAT2 genotypes to the risk of INH-induced hepatotoxicity, providing novel mechanistic insight into individual AT-DILI susceptibility.