Doxorubicin유발 심장독성에 미치는 L-Carnitine의 효과

김형춘,송계용,전구석,허인회,Kim, Hyoung-Chun,Song, Ke-Yong,Jeon, Ku-Seok,Huh, In-Hoi 대한약학회 1988 약학회지 Vol.32 No.4

This study was undertaken to elucidate the role of L-carnitine on cardiotoxicity induced by doxorubicin in mice. We designed to investigate both lipoperoxidation and histopathology in experimental group. The results obtained from prior with concurrent treatment of L-carnitine showed as follows; 1) Combination of L-carnitine showed significant inhibition of lipoperoxide in liver than heart. 2) By means of electron microscopy, we obtained histological evidence that doxorubicin-induced cardiomyopathy, is prevented by L-carnitine.

Mercuric chloride 유발 신부전에서 산소유리기에 미치는 Sodium selenite의 영향

김형춘,주왕기,허인회,Kim, Hyoung-Chun,Jhoo, Wang-Kee,Huh, In-Hoi 대한약학회 1988 약학회지 Vol.32 No.4

Oxygen free radical have recently been found to mediate cell injury after ischemia in the kidney. The purpose of our study was to determine whether selenium had an effect on damge mediated by oxygen free radical in inorganic mercury induced renal failure, toxic model of renal failure. Toxic renal failure model was produced by subcutaneous injection of mercuric chloride (4mg/kg) once a day for 7 consecutive days. In additionally, coadministration of sodium selenite (1mg/kg) was performed by the same condition. As a consequence of this study, we were able to detect partially unequivocal role of selenium as below dipicted. The combination of sodium selenite showed that markedly inhibited production of superoxide radical in mercuric chloride alone. On the other hand, combined sodium selenite was unable to enhance against significantly lowered superoxide dismutase activity after mercuric chloride insult. However, simultaneous administration of sodium selenite was inclined to induce mitochondrial superoxide dismutase and catalase.

흰쥐 간발암화 과정에서의 산소유리기의 동태

김형춘(Hyoung Chun Kim),전완주(Wan Jhoo Chun),이현우(Hyun Woo Lee),권명상(Myung Sang Kwon),송계용(Kye Yong Song),주왕기(Wang Kee Jhoo) 대한약학회 1992 약학회지 Vol.36 No.2

This study investigated the hypothesis that carcinogen-induced elevation of oxyradical during the hepatocarcinogenesis in rat. The hepatic preneoplastic lesions in the Spraque-Dawley rats were induced by the carcinogen treatment such as diethylnitrosamine(DEN) and acetylaminofluorene(AAF) in combination with partial hepatectomy(PH). The liver sample was taken at 2,6,10 and 16 months after carcinogen treatments followed by PH. Carcinogen treatments initially increased the indices of oxidative damage(activities of xanthine oxidase and production rates of superoxide anion, microsomal hydrogen peroxide, hydroxyl radical ) in the liver compared to PH groups. However, cytosolic hydrogen peroxide did not change significantly throughout the full time period. Of hydrogen peroxide scavenger, the catalase was remained lower than PH groups, whereas the peroxidase was increased after carcinogen treatments. Morphologically, the immunohistochemical analysis with glutathione-S-transferase of a placenta form(GSTP) antibody was used to detect the induction of preneoplastic nodules. During the hepatocarcinogenesis, both production rate of hydroxyl radical and activity of glutathione-S-transferase(GST) markedly increased with the appearance of the preneoplastic nodule. These results indicated that the hydroxyl radical of reactive oxygen species seemed to have a major influence on the hepatocarcinogenesis and the effect of time after removal of the carcinogen also appeared to be highly critical in the hepatocarcinogenesis.

사염화탄소와 Monosodium-L-Glutamate 병용투여에 의한 간조직의 환원형글루타치온 함량 및 그의 관련효소활성의 변화

김형춘(Hyoung Chun Kim),이왕섭(Wang Seop Lee),전완주(Wan Jhoo Chun),김수희(Soo Hee Kim),주왕기(Wang Kee Jhoo) 大韓藥學會 1991 약학회지 Vol.35 No.5

To explore the effect of monosodium-L-glutamate(MSG) on CCl4-damaged liver in Wister male rat, 5% MSG solution as drink water were administered after S.C. injection of 0.1 mg/kg CCl4 twice a week for 4 weeks. After last administration of MSG, heptic glutathione(GSH) dependent system was assayed. It showed that MSG increased significantly hepatic glutathione(GSH) and glutathione peroxidase(GSHpx), but decreased glutathione-S-transferase(GST) activity in normal rats. MSG increased significantly the GSHpx. and GST activities in rats with CCl4-induced liver damage. These results indicate that decrease of GSH dependent systems in CCl4 liver injury might be partially elevated by coadministration of MSG.

사염화탄소 및 에탄올에 의해 유도된 만성간 손상에 미치는 말로틸레이트의 항산화 작용

김형춘(Hyoung Chun Kim),허인회(In Hoi Hur) 대한약학회 1990 약학회지 Vol.34 No.4

To achieve a better understanding of antioxidant action manifested by malotilate, the dithiol malonates, we monitored the oxy radical-scavenging system against the chronic hepatic damage induced by CCl4 alone or in combination with ethanol. Malotilate was given orally at a dose of 100mg/kg/day and CCl4 1.5ml/kg was injected subcutaneously twice a week for 4 weeks. In each group receiving ethanol, drinking water was replaced by 20% aqueous solution or glucose, isocaloric amounts of ethanol, as a control of ethanol was diluted in its drinking water. Each rat was killed as a starved state at 18 hours after the period of the experiment, four weeks. The results were summarized as follows: 1)Malotilate inhibited the rate of generation of superoxide radicals, the accumulation of lipoperoxides, and promoted the synthesis of glutathione in the liver. 2)Malotilate stimulated the enhancement of activity of superoxide dismutase in hepatic mitochondria. 3)Malotilate had no effects on the hepatic H202 contents. 4)Malotilate showed the increase of catalase activity in the liver poisoned with CCl4, and also gave a tendency to increase it in the liver intoxicated with ethanol. Thus, our data suggested that the activation of hepatic antioxidant system in the presence of malotilate would play a role in protecting liver against the toxic effects of oxy radical and/or lipid peroxides under the hepatotoxic conditions induced by CCl4 with or without ethanol. However, the effects of malotilate against the ethanol-induced hepatotoxicity appear to be insignificant.

사염화탄소-유발지방간에 대한 L-글루탐산 일나트륨의 보호작용

김형춘(Hyoung Chun Kim),이왕섭(Wang Seop Lee),전완주(Wan Jhoo Chun),최용순(Yong Soon Choi),김수희(Soo Hee Kim),이현우(Hyun Woo Lee),주왕기(Wang Kee Jhoo) 대한약학회 1992 약학회지 Vol.36 No.1

To achieve better understanding of the effects of monosodium-L-glutamate(MSG) against CCl4 fatty liver in Wister male rats, 5% MSG solution was given as drinking water and CCl4 0.1ml/kg was injected subcutaneously twice a week for four weeks. It was showed that increased hepatic phospholipid and hepatic triacylglycerol levels by CCl4 challenge were significantly decreased by additional MSG, respectively. However, MSG had no apparent effect on the elevated hepatic cholesterol level in the presence of CCl4. Histrionically, additional MSG markedly inhibited fatty degeneration, spotty necrosis, inflammation and periportal vascular proliferation manifested by CCl4. respectively. Theses results indicated that effects of MSG against CCl4 induced-fatty liver appeared to be involved with partial restoration of altered hepatic lipid composition.

6-Hydroxydopamine 유발 SH-SY5Y 세포주 손상에 대한 resveratrol의 신경보호 효과

장건천,김형춘,위명복,Chang, Geon-Cheon,Kim, Hyoung-Chun,Wie, Myung-Bok 대한수의학회 2014 大韓獸醫學會誌 Vol.54 No.1

Parkinson's disease is known to exhibit progressive degeneration of the dopaminergic neurons in the substantia nigra via inhibition of glutathione metabolism. It is well known that 6-Hydroxydopamine (6-OHDA) induces Parkinson's disease-like symptoms, while resveratrol (3,5,4'-trihydroxystilbene) has been shown to have anti-inflammatory and antioxidant effects. In the present study, we investigated the neuroprotective effects of resveratrol, a phytoalexin found in grapes and various plants, on 6-OHDA-induced cell damage to the SH-SY5Y human neuroblastoma cell line. Resveratrol (5 and 10 ${\mu}M$) inhibited 6-OHDA (60 ${\mu}M$)-induced cytotoxicity in SH-SY5Y cells and induced a reduction of the number of apoptotic nuclei caused by 6-OHDA treatment. Additionally, the total apoptotic rate of cells treated with both resveratrol (10 ${\mu}M$) and 6-OHDA (60 ${\mu}M$) was less than that of 6-OHDA treated cells. Resveratrol also dose-dependently (1, 5 and 10 ${\mu}M$) scavenged reactive oxygen species (ROS) induced by 6-OHDA in SH-SY5Y cells and prevented depletion of glutathione in response to the 6-OHDA-induced cytotoxicity in the glutathione assay. Overall, these results indicate that resveratrol exerts a neuroprotective effect against 6-OHDA-induced cytotoxicity of SH-SY5Y cells by scavenging ROS and preserving glutathione.

Control Mechanisms of Ovulation by Pituitary Adenylate Cyclase-Activating Polypeptide

이여일,김형춘,김미영,전상영,Lee, Yu-Il,Kim, Hyoung-Choon,Kim, Mi-Young,Chun, Sang-Young The Korean Society for Reproductive Medicine 2005 Clinical and Experimental Reproductive Medicine Vol.32 No.2

배 경: Pituitary adenylate cyclase-activating polypeptide (PACAP)은 양의 시상하부에서 추출된 신경펩타이드 호르몬으로 난소에도 존재하여 배양된 과립막 세포에서 스테로이드합성과 cyclic AMP 형성을 촉진함이 보고되었다. 목 적: 흰쥐 난소를 실험 모델로 사용하여 배란시 황체화호르몬 (luteinizing hormone; LH)에 의해 유도된 PACAP과 PACAP 수용체의 유전자 발현양상과 신호 전달경로를 규명하고자 하였다. 재료 및 방법: 미성숙 흰쥐의 배란전 난포를 체외 배양하면서 LH로 처리하고 PACAP 및 PACAP수용체의 유전자 발현을 보기 위해서는 Northern blot 분석과 in situ hybridization (ISH)을, 그리고 단백질 수준의 PACAP 검색을 위해서는 enzyme linked immunosorbent assay (ELISA) 분석을 이용하였다. 결 과: LH 처리 후 Northern blot상의 PACAP 유전자 발현은 6~9시간에 일시적으로 최고치에 도달하였으며 ISH로 보아 과립막 세포에서 발현됨을 알 수 있었다. ELISA 분석 상 PACAP 단백질도 LH처리 후 6~12시간에 최고치를 나타내었으며, PACAP 수용체 mRNA 역시 3~9시간에 최고치로 과립막 세포에서 발현되었다. Adenylate cyclase (AC) 억제제인 MDL12330A 처리시 LH로 발현된 PACAP mRNA가 감소되며, AC의 활성제인 forskolin 처리에는 LH시와 유사한 PACAP mRNA의 발현양상을 나타내었다. 그러나 protein kinase C (PKC)의 억제제인 chelerythrine과 2-0-tetradecanolphorbol-13-acetate (TPA) 처리로는 PACAP 의 유전자 발현에 영향을 주지 못하였다. 5-lipoxygenase의 억제제인 MK886이나 nordihydroguaiaretic acid (NDGA)로 처리한 결과 LH로 유도된 PACAP 유전자의 발현이 감소되었으나, cyclooxygenase의 억제제인 indomethacin은 별로 영향을 주지 못하였다. MEK와 p38의 억제제인 PD98059와 SB203580도 LH로 촉진 된 PACAP의 유전자 발현을 농도 의존적으로 억제하였다. 결 론 : 배란전 난포에서 PACAP과 PACAP 수용체의 유전자 발현은 모두 LH의 폭발적 분비에 의해 유도되어 일시적으로 과립막 세포에서 나타나 배란을 위한 국소적인 조절 작용을 할 것으로 추정되며, LH로 촉진된 PACAP 유전자 발현을 위한 신호전달은 cAMP-PKA, lipoxygenase 및 MAP kinase 경로를 통하는 것으로 사료된다.

Analysis of the Genotypes of Panax ginseng Cultivars and Wild-Simulated Ginseng using Fluidigm SNP Chips

Mi Sun Lee(이미선),Ju Hyeok Kim(김주혁),Jin Su Gil(길진수),Jae Kyung Byun(변재경),Hyoung Chun Kim(김형춘),Sung Kwon Ko(고성권),Yi Lee(이이) 한국약용작물학회 2022 한국약용작물학회 학술대회논문집 Vol.2022 No.1

오가피속 식물 잎의 식물화학 성분 비교

안혜정(Hye Jung An),육창수(Chang Soo Yook),김형춘(Hyoung Chun Kim),고성권(Sung Kwon Ko) 대한약학회 2017 약학회지 Vol.61 No.2

This study was carried out to obtain the basic information that can be used to index Acanthopanax in ten species of China and Korea. The phytochemical components from the leaf of Acanthopanax species, were measured by the HPLC analysis. Protocatechuic acid, eleutheroside B, eleutheroside E, scopolin, rutin, hyperoside, chiisanoside, oleanolic acid were found in ethanol extracts from the leaf of Acanthopanax species. Total phenolic compound of Acanthopanax sieboldianum leaf (2.499%) was about 5.7 times higher than those of Acanthopanax divaricatus f. flavi-flos leaf (0.436%). As a result, the order of the rutin content was 1) A. sieboldianum (2.019%), 2) A. gracillstylus (1.087%), 3) A. seoulense (0.073%). On the other hand, total terpenoid of Acanthopanax divaricatus leaf (9.602%) from Korea was about 24 times higher than those of A. gracillstylus leaf (0.400%) from China. And also, the order of the chiisanoside content was 1) A. divaricatus (9.190%), 2) A. senticosus var. subinermis (8.767%), 3) A. divaricatus f. distigmatis (8.743%).