http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Hwang Gwi Ja,Roh Jongtae,Son Sangkeun,Lee Byeongsan,Jang Jun-Pil,Hur Jae-Seoun,Hong Young-Soo,Ahn Jong Seog,Ko Sung-Kyun,Jang Jae-Hyuk 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.11
A recently bioinformatic analysis of genomic sequences of fungi indicated that fungi are able to produce more secondary metabolites than expected. Despite their potency, many biosynthetic pathways are silent in the absence of specific culture conditions or chemical cues. To access cryptic metabolism, 108 fungal strains isolated from various sites were cultured with or without Streptomyces sp. 13F051 which mainly produces trichostatin analogues, followed by comparison of metabolic profiles using LC-MS. Among the 108 fungal strains, 14 produced secondary metabolites that were not recognized or were scarcely produced in mono-cultivation. Of these two fungal strains, Myrmecridium schulzeri 15F098 and Scleroconidioma sphagnicola 15S058 produced four new compounds (1-4) along with a known compound (5), demonstrating that all four compounds were produced by physical interaction with Streptomyces sp. 13F051. Bioactivity evaluation indicated that compounds 3-5 impede migration of MDA-MB-231 breast cancer cells.
Jang, Jun-Pil,Hwang, Gwi Ja,Jang, Mina,Takahashi, Shunji,Ko, Sung-Kyun,Osada, Hiroyuki,Jang, Jae-Hyuk,Ahn, Jong Seog American Chemical Society and American Society of 2018 Journal of natural products Vol.81 No.9
<P>A chemical investigation of a culture extract from a soil-derived <I>Streptomyces</I> sp. RK88-1441 led to the isolation and characterization of two new glycosylated anthraquinones, aturanosides A (<B>1</B>) and B (<B>2</B>), and a new anthraquinone derivative, aturanocin (<B>3</B>). The structures of these compounds were elucidated by detailed NMR and MS spectroscopic analyses. The absolute configurations of the sugar units, based on the magnitudes of the coupling constants, ROESY correlations, and chemical derivatization, from <B>1</B> and <B>2</B> are 6-<I>O</I>-[<I>N</I>-acetyl-α-<SMALL>D</SMALL>-glucosamino-(1→2)-α-<SMALL>L</SMALL>-rhamnoside] and 6-<I>O</I>-α-<SMALL>L</SMALL>-rhamnoside, respectively. Compounds <B>1</B> and <B>2</B> showed no cytotoxicity against human umbilical vein endothelial cells (HUVECs), but significantly suppressed vascular endothelial growth factor (VEGF)-induced tube formation and invasion of HUVECs. The down-regulation of both the phosphorylation of VEGF receptor 2 and the expression of vascular endothelial cadherin at the protein level were also observed.</P> [FIG OMISSION]</BR>
Jang, Jun-Pil,Hwang, Gwi Ja,Kwon, Min Cheol,Ryoo, In-Ja,Jang, Mina,Takahashi, Shunji,Ko, Sung-Kyun,Osada, Hiroyuki,Jang, Jae-Hyuk,Ahn, Jong Seog American Chemical Society and American Society of 2018 Journal of natural products Vol.81 No.4
<P>Two new cyclic peptides, pentaminomycins A (<B>1</B>) and B (<B>2</B>), were isolated from cultures of <I>Streptomyces</I> sp. RK88-1441. Based on the interpretation of the NMR, UV, IR, and MS data, the planar structures of <B>1</B> and <B>2</B> were elucidated as cyclic pentapeptides with a modified amino acid residue, <I>N</I><SUP>5</SUP>-hydroxyarginine (N5-OH-Arg). The absolute configurations of the constituent amino acid residues were determined by the advanced Marfey’s method. Localization of <SMALL>L</SMALL>- and <SMALL>D</SMALL>-amino acids in the sequence was ascertained by chiral analysis of the fragment peptide obtained from a partial hydrolysate; amino acids were identified by LC-MS. Pentaminomycin A (<B>1</B>) reduced α-MSH-stimulated melanin synthesis by suppressing the expression of melanogenic enzymes including tyrosinase, tyrosinase-related protein-1 (TRP-1), and tyrosinase-related protein-2 (TRP-2).</P> [FIG OMISSION]</BR>
Physicochemical Properties of β-Glucan from Acid Hydrolyzed Barley
Sang Hoon Lee,Gwi Yeong Jang,In Guk Hwang,Hyun Young Kim,Koan Sik Woo,Kee Jong Kim,Mi Ja Lee,Tae Jip Kim,Junsoo Lee,Heon Sang Jeong 한국식품영양과학회 2015 Preventive Nutrition and Food Science Vol.20 No.2
This study was performed to investigate changes in the content and purity, as well as physical characteristics of β-glucan extracted from acid hydrolyzed whole grain barleys. Waxy and non-waxy barleys (Hordeum vulgare) were hydrolyzed with different concentrations of HCl (0.1~0.5 N) for 1 h. As the HCl concentration increased, the contents of total and soluble β-glucan from acid hydrolyzed barley decreased. However the ratio of soluble/total β-glucan content and purities of β-glucan significantly increased. The ratio of β-(1→4)/β-(1→3) linkages, molecular weight, and viscosity of soluble β-glucan of raw barleys were 2.28~2.52, 6.0~7.0×10<SUP>5</SUP> g/mol, and 12.8~32.8 centipoise (cP). Those of isolated soluble β-glucan were significantly decreased to 2.05~2.15, 6.6~7.8×10<SUP>3</SUP> g/mol, and 3.6~4.2 cP, respectively, with increasing acid concentration. The re-solubility of raw barley β-glucan was about 50%, but increased to 97% with increasing acid concentration. Acid hydrolysis was shown to be an effective method to produce β-glucan with high ratio of soluble β-glucan content, purity, water solubility, and low viscosity.
Physicochemical Properties of β-Glucan from Acid Hydrolyzed Barley
Lee, Sang Hoon,Jang, Gwi Yeong,Hwang, In Guk,Kim, Hyun Young,Woo, Koan Sik,Kim, Kee Jong,Lee, Mi Ja,Kim, Tae Jip,Lee, Junsoo,Jeong, Heon Sang The Korean Society of Food Science and Nutrition 2015 Preventive Nutrition and Food Science Vol.20 No.2
This study was performed to investigate changes in the content and purity, as well as physical characteristics of ${\beta}$-glucan extracted from acid hydrolyzed whole grain barleys. Waxy and non-waxy barleys (Hordeum vulgare) were hydrolyzed with different concentrations of HCl (0.1~0.5 N) for 1 h. As the HCl concentration increased, the contents of total and soluble ${\beta}$-glucan from acid hydrolyzed barley decreased. However the ratio of soluble/total ${\beta}$-glucan content and purities of ${\beta}$-glucan significantly increased. The ratio of ${\beta}-(1{\rightarrow}4)/{\beta}-(1{\rightarrow}3)$ linkages, molecular weight, and viscosity of soluble ${\beta}$-glucan of raw barleys were 2.28~2.52, $6.0{\sim}7.0{\times}10^5g/mol$, and 12.8~32.8 centipoise (cP). Those of isolated soluble ${\beta}$-glucan were significantly decreased to 2.05~2.15, $6.6{\sim}7.8{\times}10^3g/mol$, and 3.6~4.2 cP, respectively, with increasing acid concentration. The re-solubility of raw barley ${\beta}$-glucan was about 50%, but increased to 97% with increasing acid concentration. Acid hydrolysis was shown to be an effective method to produce ${\beta}$-glucan with high ratio of soluble ${\beta}$-glucan content, purity, water solubility, and low viscosity.
열처리 온도에 따른 보리 β-Glucan의 이화학적 특성
이상훈(Sang Hoon Lee),장귀영(Gwi Yeong Jang),김현영(Hyun Young Kim),우관식(Koan Sik Woo),황인국(In Guk Hwang),김기종(Kee Jong Kim),이미자(Mi Ja Lee),김태집(Tae Jip Kim),이준수(Junsoo Lee),정헌상(Heon Sang Jeong) 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.12
열처리에 따른 보리 β-glucan의 이화학적 특성변화를 살펴보기 위하여 새쌀보리, 새찰쌀보리 및 흰찰쌀보리를 110℃~150℃에서 2시간 열처리하였으며, 총 및 수용성 β-glucan 함량, 순도, 분자량, 점도 및 재용해율을 살펴보았다. 3품종 보리의 총 β-glucan 함량은 7.77~8.40% 범위였으며, 열처리 온도가 증가함에 따라 11.59~14.65% 범위로 증가하였으나, 140℃와 150℃에서는 6.30~8.33% 범위로 감소하였다. 수용성 β-glucan의 함량은 무처리의 4.14~4.79% 범위에서 열처리 온도가 증가함에 따라 6.11~10.29%까지 증가하였으며, 150℃에서는 3.01~5.60% 범위로 감소하였다. 수용성 β-glucan의 순도는 3품종 모두 130℃까지는 31~37% 범위이었으나 140℃ 이상에서는 91%까지 증가하였다. 분자량은 메성보리보다 찰성보리가 더 컸으며, 열처리 온도가 증가함에 따라 새쌀보리는 606,463 Da에서 1,404 Da으로, 새찰쌀보리는 698,541 Da에서 1,617 Da으로, 그리고 흰찰쌀보리는 669,539 Da에서 1,550 Da으로 감소하였다. β-Glucan 수용액의 점도는 열처리 온도가 증가함에 따라 감소하였으며, 메성보리보다 찰성보리가 높았고 흰찰쌀보리보다 새찰쌀보리가 높았다. 재용해율은 무처리의 50~55% 범위에서 열처리 온도가 증가함에 따라 증가하여 150℃에서는 96.75~97.99% 범위로 증가하였다. This study was performed to investigate the changes of total and soluble β-glucan contents, purity, and physical characteristics of three heated barley varieties: Saessalbori (SSB), Saechalssalbori (SCSB), and Hinchalssalbori (HCSB). The barleys were heated at different temperatures of 110, 120, 130, 140 and 150℃ for 2 hours. The total β-glucan contents of raw SSB, SCSB, and HCSB were 8.40, 7.77 and 8.28%, and the soluble β-glucan contents were 4.79, 4.14, and 4.61%, respectively. After heating at 130℃, the total β-glucan contents increased to 11.59, 14.6, and 13.36%, as did the soluble β-glucan contents to 4.21, 7.96, and 7.23%, respectively. The purities of soluble β-glucan of the raw barleys were 35.11, 32.74 and 25.62%, but after heating at 150℃, it increased to 83.43, 91.02, and 88.01%, respectively. The molecular weight and viscosity of the β-glucan solution decreased with increasing heating temperature. The re-solubility of raw barley β-glucan was about 50%, but it was increased to 97% with increasing heating temperature. These results suggest that heating of β-glucan can improve the utilization of barley β-glucan.
Kim, Jong Won,Ko, Sung-Kyun,Kim, Hye-Min,Kim, Gun-Hee,Son, Sangkeun,Kim, Gil Soo,Hwang, Gwi Ja,Jeon, Eun Soo,Shin, Kee-Sun,Ryoo, In-Ja,Hong, Young-Soo,Oh, Hyuncheol,Lee, Kyung Ho,Soung, Nak-Kyun,Hashi American Chemical Society and American Society of 2016 Journal of natural products Vol.79 No.10
<P>Two new phenylspirodrimane derivatives, stachybotrysin (1) and stachybotrylactone B (2), were isolated from the cultures of the marine derived fungus Stachybotrys sp. KCB13F013. The structures were determined by analyzing the spectroscopic data (1D and 2D NMR and MS) and chemical transformation, including the modified Mosher's method and single-crystal X-ray structure analysis. Compound 1 exhibited an inhibitory effect on osteoclast differentiation in bone marrow macrophage cells via suppressing the RANKL-induced activation of p-ERK, p-JNK, p-p38, c-Fos, and NFATc1.</P>