고종시 감나무 부위별 수용성 추출물의 항산화 및 항염 활성

전인화(In Hwa Jeon),강현주(Hyun Ju Kang),이현서(Hyun-Seo Lee),신준호(Jun Ho Shin),박용균(Yong Gyoun Park),정승일(Seung-Il Jeong),장선일(Seon Il Jang) 한국식품과학회 2014 한국식품과학회지 Vol.46 No.4

본 연구는 고종시 감나무 부위별 수용성 추출물의 항산화 및 항염증에 대한 효과를 조사하였다. 감꼭지와 감잎은 DPPH, ABTS 및 NO 등 라디칼을 효과적으로 제거하는 활성뿐만 아니라 환원력도 우수한 항산화 활성을 보였다. 또한 LPS에 의해 활성화된 설치류 유래 대식세포주인 RAW 264.7 세포에서 NO 생성억제는 감꼭지 추출물이 가장 우수하였지만, PGE₂ 생성의 경우 모든 부위의 추출물에서 탁월한 억제 효과가 있었다. 더욱이 활성화된 RAW 264.7 세포에서 감꼭지, 감잎 및 감껍질 추출물은 농도에 의존적으로 TNF-α와 IL-1β를 효과적으로 억제하였다. 이러한 결과는 고종시 감나무 부위별 추출물 중 감꼭지와 감잎은 항산화제뿐만 아니라 항염증에 효과적인 물질이라는 것을 제시해주었고, 감껍질의 경우 비록 항산화 효과는 낮았지만, 우수한 항염효과를 나타내는 물질임을 제시해주었다. 그러므로 고종시 감나무수용성 추출물은 항산화와 항염 기능을 발휘할 수 있는 식품으로 활용될 수 있을 것이라 사료된다. Kojongsi persimmon (Diospyros kaki L.) is the major cultivar of dried persimmon in Korea. The purpose of this study was to investigate the antioxidant and anti-inflammatory activities of water-soluble extracts from the calyx (PCE), peel (PPE) and leaf (PLE) of Kojongsi persimmon. PCE showed the highest total phenolic and flavonoid contents. In addition, the antioxidant activities (diphenylpicrylhydrazyl, 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), nitric oxide and reducing power) of PCE were higher than those of PPE and PLE. Moreover, PCE, PPE and PLE significantly suppressed the production of inflammatory mediators (nitric oxide and prostaglandinE₂) and pro-inflammatory cytokines (tumor necrosis factor-α and interluekin-1β) by lipopolysaccharide-stimulated RAW 264.7 cells in a dose-dependent manner. PCE showed the highest anti-inflammatory activity. Thus, these results suggest that the calyx of Kojongsi persimmon may be highly valuable as a natural product owing to its high-quality functional components as well as its-antioxidant, antiinflammatory activities.

YCG063 inhibits Pseudomonas aeruginosa LPS-induced inflammation in human retinal pigment epithelial cells through the TLR2-mediated AKT/NF-κB pathway and ROS-independent pathways

PAENG, SUNG HWA,PARK, WON SUN,JUNG, WON-KYO,LEE, DAE-SUNG,KIM, GI-YOUNG,CHOI, YUNG HYUN,SEO, SU-KIL,JANG, WON HEE,CHOI, JUNG SIK,LEE, YOUNG-MIN,PARK, SAEGWANG,CHOI, IL-WHAN UNKNOWN 2015 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.36 No.3

<P>YCG063 is known as an inhibitor of reactive oxygen species?(ROS); however, its intracellular mechanisms of action remain poorly understood. In the present study, we investigated the effects of YCG063 on the inflammatory response of Pseudomonas aeruginosa lipopolysaccharide?(PA-LPS)?stimulated human retinal pigment epithelial cells?(RPE cells). Human adult RPE cells?(ARPE?19) were stimulated with PA-LPS. We then investigated the LPS-induced expression of several inflammatory mediators, such as interleukin?(IL)-6, IL-8, monocyte chemoattractant protein-1?(MCP-1) and intracellular adhesion molecule-1?(ICAM-1) in the ARPE-19 cells. We performed an enzyme-linked immunosorbent assay?(ELISA), western blot analysis, electrophoretic mobility shift assay?(EMSA) and fluorescence-activated cell sorting?(FACS) to elucidate the mechanisms involved in the anti-inflammatory effects of YCG063 in the PA-LPS-stimulated cells. The results revealed that treatment with YCG063 significantly inhibited the levels of IL-6, IL-8, MCP-1 and ICAM-1 in the PA-LPS-stimulated ARPE-19 cells. YCG063 also markedly inhibited the phosphorylation of AKT in the PA?LPS-stimulated cells. In addition, the activation of nuclear factor-κB?(NF-κB) was also attenuated folllowing treatment with YCG063. ROS were not generated in the PA-LPS-stimulated cells. In conclusion, our data indicate that YCG063 may prove to be a potential protective agent against inflammation, possibly through the downregulation of Toll?like receptor?2?(TLR2) and the AKT-dependent NF-κB activation pathway in PA-LPS-stimulated ARPE-19 cells. Furthermore, this anti-inflammatory activity occurred through ROS-independent signaling pathways.</P>

Monosodium Iodoacetate로 유발한 골관절염 흰쥐에 대한 여지초(荔枝草)의 효능 평가

이수화 ( Lee Soo Hwa ),서부일 ( Seo Bu-il ) 한약응용학회 2021 한약응용학회지 Vol.21 No.1

Salviae Plebeiae Herba (SP) contains a numberous chemical components and reported to exert various pharmacological activities such as antioxidant, anti-inflammatory, antimicrobial, analgesic, hypoglycemic, antiviral, anti-tumour, and hepatoprotective effects. Even though it has these various effects, its scientific proof remains uncertain the effect and underlying mechanism about joint damage. Accordingly, the present study was designed to evaluate the therapeutic effects and possible underlying mechanism of SP on osteoarthritis. OA models were induced via intra-joint injection of monosodium iodoacetate (MIA) 50 ㎕ with 80 ㎎/㎖ in rats. Excluding the normal group, OA-induced mice were divided into 4 groups (control, INDO, SPL, SPH). The drug concentrations were indomethacin 5 ㎎/㎏, SP 500 ㎎/㎏, and SP 1000 ㎎/㎏, and were administered orally once a day for 2 weeks. After drug supplementation, the effects of SP were measured with serum analysis, western blotting, and histopathological staining. SP administration improved hind limb overload and it led to relieve pain. Moreover, it suppressed oxidative related factors. The NF-κB inactivation though the inhibition of IκBα phosphorlyation significantly decreased both inflammatory mediators such as COX-2 and iNOS and cytokines such as TNF-α, IL-1β, and IL-6. Whereas, anti-inflammatory cytokines like IL-4 and IL-10 were shown to increase. In addition, SP administration exerted a protective effect on ECM by reducing the expression of collagen degradation factors including MMP-1, -3, -13 and by increasing TIMP-1. Taken together, SP may possess the effect of delaying the progression of osteoarthritis and protecting cartilage. Accordingly, the present study may provide a scientific basis the chondroprotective effect of SP on MIA induced osteoarthritis.

반도체공정기술을 이용한 마이크로 전류법적 포도당샌서의 제조 및 특성

서화일 韓國技術敎育大學校 1996 論文集 Vol.3 No.1

A micro glucose sensor based on the electrolysis of hydrogen peroxide was fabricated by forming immobilized GOD membrane on a Pt electrode, which was fabricated by semiconductor technology, and its characteristics investigated using two electrode system. The size of the fabricated sensor was 1mmX1.3mm. The sensor showed a response time of 3 minutes, a sensitivity of 50nA/mM and a good output linearity in the range of 0 to 7mM glucose concentration. These characteristics would allow the sensor to measure glucose concentration practically. For measuring glucose concentration in human blood without dilution by using the sensor, a outer membrane formation if required.

화학기상증착 반응기 시뮬레이션 연구

서화일,장호정,장영철,석창길,이은호,임장규 호서대학교반도체제조장비국산화 연구센터 2000 반도체 장비기술 논문집 Vol.2000 No.-

ISFET 바이오센서를 위한 신호처리회로의 개발과 그 요소센서와의 단일칩 집적

서화일,손병기 경북대학교 센서기술연구소 1990 센서技術學術大會論文集 Vol.1 No.1

The new signal process circuit using ISFETs as two input devices of a MOS differential amplifier stage for application to a ISFET biosesnor was developed. And the chip including developed circuit, ISFET and reference electrode was designed and fabricated according to modified LOCOS p-well CMOS process. The fabricated chip showed a gain of 0.8 and 1.6, good linearity in input-output relationship and very small power dissipation of 4mW. By forming immobilized urease membrane on ISFET gate of the chip, urea sensor system and developed signal process circuit was successfully integrated on a single chip.

ISFET의 제조공정 및 그 동작특성

이광만,서화일,손병기 경북대학교 센서기술연구소 1990 센서技術學術大會論文集 Vol.1 No.1

ISFET's fabrication process have been designed and computer simulated with SUFREM-II, and their results are good agreement with each other. ISFET chip has been fabraicated by using the ISRC's standard "3um CMOS process". A two-step TCA oxidation for the gate oxide and multilayer encapsulation using silicone rubber was specially used for the improvement of the stability of the ISFETs. The C-V measurement of gate oxide and the AES analysis of gate nitride were performed.The measured sensitivities of the pH-ISFETs are 43 mV/pH and 54 mV/pH without and with HF treatment, respectively.

FET형 마이크로센서 개발

손병기,박이순,서화일,권대혁,고광락 경북대학교 센서기술연구소 1996 연차보고서 Vol.1996 No.-

USB 키보드 컨트롤러의 FPGA구현

장영조,서화일 한국기술교육대학교 2000 論文集 Vol.7 No.1

ABSTRACT This paper represents an FPGA implementation on USB Keyboard controller. USB is a serial interface to PC with 12Mbps full speed and applications for the peripheral devices are growing very widely. The architecture is designed with high modularity to devise various applications of USB. USB keyboard is classified to HID device. The design method for HID class is shown. Complex device containing mouse and ACPI function is also implemented by means of HID report to the same USB device. The keyboard controller is designed using Verilog HDL and simulated its functionality and performance. The architecture is implemented using an Altera 10KA100 FPGA and its evaluation board. The functionality is tested with direct connection to PC host and confirmed by USB compliance test program.

FET형 마이크로센서 개발

손병기,박이순,서화일,고광락 경북대학교 센서기술연구소 1997 연차보고서 Vol.1997 No.-