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Hue, Nguyen Thi Ngoc,Derva Jarvis,Bhuwon Sthapit 한국생태학회 2004 Journal of Ecology and Environment Vol.27 No.1
Understanding farmer household and community management of crop diversity is necessary in order to effectively strengthen the local plant genetic resources management system in Vietnam. Assessment and management for distribution of farmer s cultivars of rice, taro and tropical tree species were conducted in four different agro-ecological zones in Vietnam using participatory approaches, focus surveys, diversity fairs, agro-morphological field trials, isozyme analysis to understand why Vietnamese farmers grow diverse types of these crop cultivars, when and where these crops cultivars are grown and how farmers maintain and use them. Results showed significant differences in the extent, distribution and use pattern crop cultivars diversity in contrasting environments and between community farmer households. Seed system is an important role in the access and use of diversity in eco-geographical regions.
Hue, Khuc Thi,Van, Do Thi Thanh,Ledin, Inger,Wredle, Ewa,Sporndly, Eva Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.12
The experiment studied the effect of harvesting frequencies and varieties on yield, chemical composition and hydrogen cyanide content in cassava foliage. Foliage from three cassava varieties, K94 (very bitter), K98-7 (medium bitter) and a local (sweet), were harvested in three different cutting cycles, at 3, 6 and 9 months; 6 and 9 months and 9 months after planting, in a 2-yr experiment carried out in Hanoi, Vietnam. Increasing the harvesting frequency increased dry matter (DM) and crude protein (CP) production in cassava foliage. The K94 variety produced higher foliage yields than the other two varieties. Dry matter, neutral detergent fibre (NDF), acid detergent fibre (ADF) and total tannin content increased with months to the first harvest, whereas CP content decreased. Hydrogen cyanide (HCN) content was lower at the first harvest than at later harvests for all cutting cycles. At subsequent harvests the content of total tannins tended to decline, while HCN content increased (p<0.05). Chemical composition differed somewhat across varieties except for total tannins and ash. Dry matter, NDF, ADF and total tannins were higher in fully matured leaves, while CP and HCN were lower in developing leaves.
Neurochemical Properties of Dental Primary Aff erent Neurons
Hue Vang,정지훈,김현영,박석범,정승준,김중수,오석배 한국뇌신경과학회 2012 Experimental Neurobiology Vol.21 No.2
The long belief that dental primary afferent (DPA) neurons are entirely composed of nociceptive neurons has been challenged by several anatomical and functional investigations. In order to characterize non-nociceptivepopulation among DPA neurons,retrograde transport fluorescent dye was placed in upper molars of rats and immunohistochemical detection of peripherin and neurofilament 200 in the labeled trigeminal ganglia was performed. As the results, majority ofDPA neurons were peripherinexpressing small-sized neurons, showing characteristic ofnociceptive C-fi bers. However, 25.7% of DPA were stained with antibody against neurofi lament 200, indicating signifi cant portion of DPA neurons are related to large myelinated Aβ fi bers. Th ere were a small number of neurons thatexpressed both peripherin and neurofilament 200, suggestive of Aδ fibers. The possible transition of neurochemical properties by neuronal injury induced by retrograde labeling technique was ruled out by detection of minimal expression of neuronal injury marker, ATF-3. These results suggest that in addition to the large population of C-fiber-related nociceptive neurons, a subset of DPA neurons is myelinated large neurons,which is related to low-threshold mechanosensitive Aβfi bers. We suggest that these Aβ fi ber-related neurons might play a role as mechanotransducers of fl uid movement within dentinal tubules.
Biosynthesis of fucosyllactose in engineered Corynebacterium glutamicum
Hue Thi Nguyen,Obed Jackson Amoah,Su Yeong Ma,Jae Kyung Sohng 한국당과학회 2022 한국당과학회 학술대회 Vol.2022 No.07
Human milk oligosaccharides (HMOs) have been proven to be a key ingredient found in breast milk and beneficial to infant health. Structurally, the fucose-containing oligosaccharides are major components, with a proportion of approximately 50–80% in the HMOs. In particular, 2-fucosyllactose (2’-FL) and 3-fucosyllactose (3-FL) are 2 major components of the abundant HMO component. Importantly, 2’-FL is approved for use in infant formula for improving probiotic functions by the FDA of the USA and the European Food Safety Authority, while 3-FL can be used as a prebiotic to improve the growth of beneficial microorganisms. Over the last decade, various approaches have been conducted for fucosyllactose (FL) production, including chemical synthesis, enzymatic conversion, and microbial fermentation. Most studies were performed in Escherichia coli due to the simple culture conditions and availability of various genetic tools. Besides E. coli, Bacillus subtilis and Saccharomyces cerevisiae have also been selected as the hosts for the production of FL due to their generally regarded safe (GRAS) status. As a type of GRAS strain, Corynebacterium glutamicum has been widely used in the biosynthesis of food-grade, high-value-added products, including lysine, glutamic acid, violacein and resveratrol. In this study, the biosynthetic enzymes of FL were condon-optimized and heterologously expressed in engineered C. glutamicum for the production of FL. The trace amount production of FL was confirmed by TLC, HPLC in this study.
Hue, Jin Joo,Lee, Hu-Jang,Nam, Sang Yoon,Kim, Jong-Soo,Lee, Beom Jun,Yun, Young Won The Korean Society of Veterinary Science 2015 大韓獸醫學會誌 Vol.55 No.1
To investigate kinetics of free $^{177}Lu$ and $^{177}Lu$-labeled thermally cross-linked superparamagnetic iron oxide nanoparticles (TCL-SPION), suspensions were intravenously injected into the tail vein of mice at a dose of $5{\mu}Ci$/mouse or 15 mg/kg body weight, respectively. Free $^{177}Lu$ radioactivity levels were highest in kidney followed by liver and lung 1 day post-injection. $^{177}Lu$-labeled TCL-SPION radioactivity in liver and spleen was significantly higher compared to that of other organs throughout the experimental period (p < 0.05). Radioactivity in blood, brain, and epididymis rapidly declined until 28 days. Based on these results, TCL-SPION could be a safe carrier of therapeutics.
Hue, Jin Joo,Lee, Hu-Jang,Jon, Sangyong,Nam, Sang Yoon,Yun, Young Won,Kim, Jong-Soo,Lee, Beom Jun The Korean Society of Veterinary Science 2014 大韓獸醫學會誌 Vol.54 No.4
This study was investigated the change of concentration and toxicity of thermally cross-linked superparamagnetic iron oxide nanoparticles (TCL-SPION) on tissues of Sprague-Dawley rats. TCL-SPION at the dose of 15 mg/kg body weight was intravenously injected into the tail vein of the male Sprague-Dawley rats. The fate of TCL-SPION in serum, urine and tissues was observed during 28 days. Serum iron level was maximal at 0.25 h post-injection and gradually declined thereafter. In addition, the sinusoids of liver and the red pulp area of spleen were mainly accumulated iron from 0.5 h to 28-day post-injection. In kidney, iron deposition was detected in the tubular area until 0.5 h after injection. Malondialdehyde concentration in the liver slightly increased with time and was not different with that at zero time. In the liver and spleen, TNF-${\alpha}$ and IL-6 levels of TS treated with TCL-SPION were not different with those of the control during the experimental period. From the results, TCL-SPION could stay fairly long-time in certain tissues after intravenous injection without toxicity. The results indicated that TCL-SPION might be useful and safe as a contrast for the diagnosis of cancer or a carrier of therapeutic reagents to treat diseases.