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- 저자 : Hooman Razi (검색결과 2 건)
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Roohollah Shamloo-Dashtpagerdi,Hooman Razi,Angelica Lindlof,Ali Niazi,Ali Dadkhodaie,Esmaeil Ebrahimie 한국유전학회 2013 Genes & Genomics Vol.35 No.3
Triticum monococcum has recently drawn the attention of biologists to discover and utilize novel genes and alleles. To explore the molecular features of the genetic network governing floral transition in shoot apical meristem (SAM) of spring growth habit T. monococcum,two expressed sequence tag (EST) libraries containing 3,031 ESTs from vegetative SAM (VS) and 2,647 ESTs from early reproductive SAM (RS) were analyzed. Assembly of ESTs resulted in 2,303 unigenes for VS library (368 contigs and 1,935 singletons) and 1,890unigenes (337 contigs and 1,553 singletons) for RS library. The 67.05 % of VS unigenes and 66.30 % of RS unigenes showed significant similarity with genes of known, putative and or unknown function, whereas the remaining 32.95 %of the VS unigenes and 33.7 % of RS unigenes displayed no significant match with the public protein database. The 1,064 and 866 unigenes of VS and RS libraries were assigned to functional categories using Pageman ontology tool. Further analysis revealed that the switch from VS to RS caused significant changes in the abundance of unigenes assigned to some functional categories. A total of 37genes were identified which were significantly differentially expressed between vegetative and reproductive stages of T. monococcum SAM. Investigation of the differentially expressed genes revealed the importance of the genes involved in energy metabolism, ubiquitin/26S proteasome system, polyamines biosynthesis and signaling of reactive oxygen species in SAM differentiation towards floral transition in T. monococcum.
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Ali Pourkhaloee,Morteza Khosh-Khui,Paul Arens,Hassan Salehi,Hooman Razi,Ali Niazi,Alireza Afsharifar,Jaap van Tuyl 한국원예학회 2018 Horticulture, Environment, and Biotechnology Vol.59 No.6
Tulip ( Tulipa L.) is one of the most important ornamental geophytes in the world. Analysis of molecular variability of tulipsis of great importance in conservation and parental lines selection in breeding programs. Of the 70 genic microsatellites, 15highly polymorphic and reproducible markers were used to assess the genetic diversity, structure, and relationships among280 individuals of 36 wild and cultivated tulip accessions from two countries: Iran and the Netherlands. The mean valuesof gene diversity and polymorphism information content were 0.69 and 0.66, respectively, which indicated the high discriminatorypower of markers. The calculated genetic diversity parameters were found to be the highest in wild T . systolaStapf (Derak region). Bayesian model-based STRU CTU RE analysis detected fi ve gene pools for 36 germplasms whichcorresponded with morphological observations and traditional classifi cations. Based on analysis of molecular variance, toconserve wild genetic resources in some geographical locations, sampling should be performed from distant locations toachieve high diversity. The unweighted pair group method with arithmetic mean dendrogram and principal component analysisplot indicated that among wild tulips, T . systola and T . micheliana Hoog exhibited the closest relationships with cultivatedtulips. Thus, it can be assumed that wild tulips from Iran and perhaps other Middle East countries played a role in the originof T . gesneriana , which is likely a tulip species hybrid of unclear origin. In conclusion, due to the high genetic variabilityof wild tulips, they can be used in tulip breeding programs as a source of useful alleles related to resistance against stresses.
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