http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Chemical Vapor Deposition Growth of Graphene Domains Across the Cu Grain Boundaries
Yang Wang,Yu Cheng,Yunlu Wang,Shuai Zhang,Chen Xu,Xuewei Zhang,Miao Wang,Yang Xia,Qunyang Li,Pei Zhao,Hongtao Wang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.08
Many aspects in the chemical vapor deposition (CVD) growth of graphene remain unclear such as its behavior near the catalyst grain boundaries. Here we investigate the CVD growth mechanism of graphene across the Cu grain boundaries using unidirectional aligned graphene domains, which simplifies the analysis of both graphene and Cu to a large extent. We found that for a graphene domain grown across the Cu grain boundary, the domain orientation is determined by the Cu grain where the domain nucleation center is located, and the Cu grain boundary will not change the growth behavior for this graphene domain. This growth mechanism is consistent with the Custep-attached nucleation and edge-attachment-limited growth mechanism for H-terminated graphene domains and will provide more guidance for the synthesis of high-quality graphene with less domain boundaries.
Wang, Hongtao,Sun, Lin,Luo, Chunhua,Fan, Suhua Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.5
In this study, an intermediate temperature ionic conductor, $Ce_{0.95}Eu_{0.05}P_2O_7$, was prepared by solid state reaction. The variation of conductivities with the pressure $pH_2O$ or time were studied. The highest conductivity of $Ce_{0.95}Eu_{0.05}P_2O_7$ sample was observed in dry air atmosphere at $300^{\circ}C$ to be $1.1{\times}10^{-4}S{\cdot}cm^{-1}$ and in wet air atmosphere ($pH_2O=7.4{\times}10^3Pa$) at $100^{\circ}C$ to be $1.4{\times}10^{-3}S{\cdot}cm^{-1}$, respectively. The log ${\sigma}$ ~ log ($pO_2$) plot result indicated that $Ce_{0.95}Eu_{0.05}P_2O_7$ was almost a pure ionic conductor under high oxygen partial pressure and a mixed conductor of ion and electron under low oxygen partial pressure.
Wang, Hongtao,Kwon, Woo-Saeng,Yang, Dong-Uk,Kim, Min-Kyeoung,Sathiyamoorthy, Subramaniyam,Jin, Haizhu,Yang, Deok-Chun Informa Healthcare 2011 Mitochondrial DNA Vol.22 No.1
<P><I>Background and aims.</I> Molecular authentication of Korean ginseng cultivars was investigated using the mitochondrial nicotinamide adenine dinucleotide (NADH) dehydrogenase subunit 7 (<I>nad</I>7) intron 3 region. <I>Materials and methods</I>. A mutation site specific to <I>Panax ginseng</I> “??Gumpoong”?? and “??Chungsun”?? cultivars was detected within the sequence data. Based on this mutation site and the “??Gumpoong”??-specific single nucleotide polymorphism site reported in 26S rDNA, two modified allele-specific primer pairs were designed and a multiplex amplification refractory mutation system (MARMS) was applied to identify “??Gumpoong”?? and “??Chungsun.”?? <I>Results.</I> The results showed that “??Gumpoong”?? and “??Chungsun”?? can be clearly discriminated from the other Korean ginseng cultivars by simultaneously identifying the haplotype of “??Gumpoong”?? and the specific allele of “??Chungsun”?? by applying the MARMS. <I>Conclusion.</I> This study, therefore, provides a simple and reliable method for simultaneous authentication of “??Gumpoong”?? and “??Chungsun”?? cultivars.</P>
Wang, Hongtao,Li, Guisheng,Kwon, Woo-Saeng,Yang, Deok-Chun,Zhu, Jianhua MDPI 2016 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.17 No.6
<P><I>Panax ginseng</I> is one of the most valuable medicinal plants in the Orient. The low level of genetic variation has limited the application of molecular markers for cultivar authentication and marker-assisted selection in cultivated ginseng. To exploit DNA polymorphism within ginseng cultivars, ginseng expressed sequence tags (ESTs) were searched against the potential intron polymorphism (PIP) database to predict the positions of introns. Intron-flanking primers were then designed in conserved exon regions and used to amplify across the more variable introns. Sequencing results showed that single nucleotide polymorphisms (SNPs), as well as indels, were detected in four EST-derived introns, and SNP markers specific to “Gopoong” and “K-1” were first reported in this study. Based on cultivar-specific SNP sites, allele-specific polymerase chain reaction (PCR) was conducted and proved to be effective for the authentication of ginseng cultivars. Additionally, the combination of a simple NaOH-Tris DNA isolation method and real-time allele-specific PCR assay enabled the high throughput selection of cultivars from ginseng fields. The established real-time allele-specific PCR assay should be applied to molecular authentication and marker assisted selection of <I>P. ginseng</I> cultivars, and the EST intron-targeting strategy will provide a potential approach for marker development in species without whole genomic DNA sequence information.</P>
Molecular discrimination of Panax ginseng cultivar K-1 using pathogenesis-related protein 5 gene
Wang, Hongtao,Xu, Fengjiao,Wang, Xinqi,Kwon, Woo-Saeng,Yang, Deok-Chun The Korean Society of Ginseng 2019 Journal of Ginseng Research Vol.43 No.3
Background: The mixed-cultivation of different Panax ginseng cultivars can cause adverse effects on stability of yield and quality. K-1 is a superior cultivar with good root shape and stronger disease resistance. DNA markers mined from functional genes are clearly desirable for K-1, as they may associate with major traits and can be used for marker-assisted selection to maintain the high quality of Korean ginseng. Methods: Five genes encoding pathogenesis-related (PR) proteins of P. ginseng were amplified and compared for polymorphism mining. Primary, secondary, and tertiary structures of PR5 protein were analyzed by ExPASy-ProtParam, PSSpred, and I-TASSER methods, respectively. A coding single nucleotide polymorphism (SNP)-based specific primer was designed for K-1 by introducing a destabilizing mismatch within the 3' end. Allele-specific polymerase chain reaction (PCR) and real-time allele-specific PCR assays were conducted for molecular discrimination of K-1 from other cultivars and landraces. Results: A coding SNP leading to the modification of amino acid residue from aspartic acid to asparagine was exploited in PR5 gene of K-1 cultivar. Bioinformatics analysis showed that the modification of amino acid residue changed the secondary and tertiary structures of the PR5 protein. Primer KSR was designed for specific discrimination of K-1 from other ginseng cultivars and landraces. The developed real-time allele-specific PCR assay enabled easier automation and accurate genotyping of K-1 from a large number of ginseng samples. Conclusion: The SNP marker and the developed real-time allele-specific PCR assay will be useful not only for marker-assisted selection of K-1 cultivar but also for quality control in breeding and seed programs of P. ginseng.
Studies on Ionic Conduction in Ce0.95Eu0.05P2O7 at Intermediate Temperatures
Hongtao Wang,Lin Sun,Chunhua Luo,Suhua Fan 대한화학회 2014 Bulletin of the Korean Chemical Society Vol.35 No.5
In this study, an intermediate temperature ionic conductor, Ce0.95Eu0.05P2O7, was prepared by solid state reaction. The variation of conductivities with the pressure pH2O or time were studied. The highest conductivity of Ce0.95Eu0.05P2O7 sample was observed in dry air atmosphere at 300 °C to be 1.1 × 10−4 S·cm−1 and in wet air atmosphere (pH2O = 7.4 × 103 Pa) at 100 °C to be 1.4 × 10−3 S·cm−1, respectively. The log σ ~ log (pO2) plot result indicated that Ce0.95Eu0.05P2O7 was almost a pure ionic conductor under high oxygen partial pressure and a mixed conductor of ion and electron under low oxygen partial pressure.