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장석민,강재구,최기명,김홍래,진동일 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.1
This study was conducted to evaluate the response of CIDR-S on estrus synchronization of dairy goats and the effect of artificial insemination on conception rate. Estrus synchronization of sexually matured Saanen goats was induced with insertion of CIDR-S into vagina for 15 days and injection of 500 IU PMSG before removal of CIDR-S. Artificial insemination was conducted using liquid or frozen semen by intra-cervical insemination with synchronized does. Estrus synchronization was 90% using CIDR-S insertion for 15 days. Conception rate of inseminated does was 20% - 25%, while that of natural mated does was 100%. These results suggested that synchronization of dairy goats can be successfully induced and artificial insemination method has to be improved for the practical use in dairy goats.
Malignant Glioma with Neuronal Marker Expression : A Clinicopathological Study of 18 Cases
Kim, Hong Rye,Lee, Jae Jun,Lee, Jung-Il,Nam, Do Hyun,Suh, Yeon-Lim,Seol, Ho Jun The Korean Neurosurgical Society 2016 Journal of Korean neurosurgical society Vol.59 No.1
Objective : Malignant gliomas with neuronal marker expression (MGwNM) are rare and poorly characterized. Increasingly diverse types of MGwNM have been described and these reported cases underscore the dilemmas in the classification and diagnosis of those tumors. The aim of this study is to provide additional insights into MGwNM and present the clinicopathological features of 18 patients. Methods : We reviewed the medical records of 18 patients diagnosed as MGwNM at our institute between January 2006 and December 2012. Macroscopic total resection was performed in 11 patients (61%). We evaluated the methylation status of $O^6$-methylguanine-DNA methyltransferase (MGMT) and expression of isocitrate dehydrogenase 1 (IDH-1) in all cases, and deletions of 1p and 19q in available cases. Results : The estimated median overall survival was 21.2 months. The median progression-free survival was 6.3 months. Six patients (33%) had MGMT methylation but IDH1 mutation was found in only one patient (6%). Gene analysis for 1p19q performed in nine patients revealed no deletion in six, 19q deletion only in two, and 1p deletion only in one. The extent of resection was significantly correlated with progression free survival on both univariate analysis and multivariate analysis (p=0.002 and p=0.013, respectively). Conclusion : In this study, the overall survival of MGwNM was not superior to glioblastoma. The extent of resection has a significant prognostic impact on progression-free survival. Further studies of the prognostic factors related to chemo-radio therapy, similar to studies with glioblastoma, are mandatory to improve survival.
A two-dimensional electrophoresis reference map for the bovine placenta during late pregnancy
Kim, Hong Rye,Han, Rong Xun,Yoon, Jong Taek,Park, Chang Sik,Jin, Dong Il WILEY-VCH Verlag 2010 Proteomics Vol.10 No.3
<P>An understanding of bovine placental gene expression is essential for the study of animal reproductive physiology. Recent reports have found that placental abnormalities occur frequently in cloned bovines and mice. However, the molecular mechanisms underlying bovine placenta function remain unclear. Here, we present a preliminary description of the bovine placenta proteome. Proteins within the isoelectric point ranging from 4.0 to 7.0 and 6.0 to 9.0 were analyzed separately using 2-DE, using three replicates of bovine placenta. Approximately 2000 spots were detected in a placental 2-D gel stained with Coomassie blue. Subsequent excision of 380 spots from gels and MALDI-TOF MS analysis allowed the identification of 273 proteins. Our results revealed the composite profiles of key proteins in the bovine placenta during late pregnancy. These protein profiles will shed light on placental function during pregnancy and assist with functional analysis of the proteins.</P>
Kim, Baek-Chul,Kim, Hong-Rye,Kim, Myung-Yoon,Park, Chang-Sik,Jin, Dong-Il The Korean Society of Animal Reproduction 2009 Reproductive & developmental biology Vol.33 No.2
Animals produced by somatic cell nuclear transfer (SCNT) using genetically modified cells are almost always transgenic, implying that this method is more efficient than the traditional pronuclear microinjection method. Most somatic cells for SCNT in animals are fetus-derived primary cells and successful gene integration in somatic cells will depend on transfection condition. The objective of this study is to evaluate the efficiency of electroporation (Microporator) and liposome reagents (F-6, F-HD, W-EX, W-Q, W-M) for tissue-type plasminogen activator (tPA) gene transfection and to estimate the overall efficiency of transfection of Korean native pig fetal fibroblast cells (KNPFF). Electroporation showed significantly higher transfection efficiency than liposome reagents with regard to the transfection of in vitro cultures in the early stages of development (41.7% with Microporator vs. 18.3% with F-6, 20.0% with F-HD 18.5% with W-EX, 5.0% with W-M and 6.3% W-Q,). Colonies identified as tPA-positives were treated once more with G418 for 10 to 14 days and growing colonies were selected again. When the cells of newly selected colonies were subjected to single-cell PCR, reselection of colonies following second round of G418 selection increased the rate of transgene integration per each colony. These results suggest that transfection with electroporation is the most efficient and the second rounds of G418 selection may be an effective method for transfection of porcine fetal fibroblast cells.
Kim, Hong-Rye,Han, Rong-Xun,Lee, Hye-Ran,Yoon, Jong-Taek,Cheong, Hee-Tae,Park, Chang-Sik,Jin, Dong-Il 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10
The objective of this study was to analyzed pattern of proteins expression abnormally in cloned bovine placenta. TIMP-2 protein whose function is related to extracellular matrix degradation and tissue remodeling processes was one of differentially up-regulated proteins in SCNT placenta. And one of down-regulated protein in SCNT placenta was identified as vimentin protein that is presumed to stabilize the architecture of the cytoplasm. The expression patterns of these proteins were validated by Western blotting. To evaluate how regulatory loci of TIMP-2 and vimentin genes was programmed reprogramming in cloned placenta, the status of DNA methylation in the promoter region of TIMP-2 and vimentin genes was analyzed by sodium Bisulfite mapping. The DNA methylation results showed that there was not difference in methylation pattern of TIMP-2 and vimentin loci between cloned and normal placenta. Histone H3 acetylation state of the nucleosome was analyzed in the cloned placental and normal placenta by Western blotting. A small portion of the protein lysates were subjected to Western blotting with the antibodies against anti acetyl-Histone H3. Overall histone H3 acetylation state of SCNT placenta was significantly higher than those of normal placenta cells. It is postulated that cloned placenta at the end of gestation seems to be unusual in function and morphology of placenta via improper expression of TIMP-2 and vimentin by abnormal acetylation states of cloned genome.