홍씨 앞방각측정법의 임상적 유용성

강신희,박기호,홍철,김동명,Shin Hee Kang,M,D,Ki Ho Park,M,D,Chul Hong,M,D,Dong Myoung Kim,M,D 대한안과학회 2007 대한안과학회지 Vol.48 No.3

Purpose: To compare the Hong`s grading method, utilized for slit-lamp grading of the mid-peripheral angle, with other conventional grading methods. Actual angle was measured by ultrasound biomicroscopy (UBM) for comparison. Methods: Fifteen eyes with narrow angles and eighteen eyes with wide angles were studied. The average age of thirty-three subjects was 46.8±19.2, and the male to female ratio was 1:3.7. The mid-peripheral angle was graded by Hong`s, van Herick`s and Spaeth`s grading system. The actual anterior chamber angle was evaluated by UBM and the correlation between each grading system and actual angle was investigated. Results: The results from the Hong`s grading (r=0.802, R2=0.643, p<0.01), Spaeth`s grading (r=0.728, R2=0.530, p<0.01) and van Herick`s grading (r=0.618, R2=0.382, p<0.01) methods correlated significantly with the actual angles. Conclusions: The Hong`s grading method was an easy and effective tool to evaluate anterior chamber angle. In addition, the results of the Hong`s grading method were closer to the actual angle than the other methods.

Nasal-type NK/T-cell lymphomas are more frequently T rather than NK lineage based on T-cell receptor gene, RNA, and protein studies: lineage does not predict clinical behavior

Hong, M.,Lee, T.,Young Kang, S.,Kim, S. J.,Kim, W.,Ko, Y. H. Nature Publishing Group 2016 Modern pathology Vol.29 No.5

<P>Extranodal natural killer (NK)/T-cell lymphoma (ENKTL), nasal type, comprises NK or cytotoxic T cells. We evaluated the clinical impact of cell type and the usefulness of T-cell receptor (TCR) gene transcripts in distinguishing cell lineage. One hundred and eight cases of ENKTL were analyzed for TCR gene rearrangements using the BIOMED-2 protocol and for TCR gene expression using immunohistochemistry for TCR-beta F1 and TCR-c gamma M1, and RNA in situ hybridization for TCR gene transcripts. Prognostic factors were analyzed. Among the 108 cases, 44 were monoclonal for a TCR rearrangement (40%) while 64 (60%) were undefinable. The monoclonal cases expressed TCR-beta F1 in 14 out of 40 cases (35%) and TCR-c gamma M1 in 1 out of 44 cases (2%). The 64 undetermined cases expressed TCR-beta F1 in 15 cases (23%) and TCR-c gamma M1 in 1 (2%). Thirteen of 40 TCR-beta constant gene transcript-positive cases (33%) expressed TCR-beta F1 and one of nine TCR-gamma constant gene transcript-positive cases (11%) expressed TCR-c gamma M1. TCR gene transcripts were not useful in the distinction of cell lineages. TCR gene transcripts were positive in ENKTLs as well as in normal B cells and aggressive NK-cell leukemia. Based on gene rearrangements and immunohistochemistry for TCR, there were 60 T-cell type cases (56%), 32 NK-cell type cases (30%), and 16 cases with an undetermined cell type (14%). TCR protein was expressed in 30/60 T-ENKTLs (50%) in a variable fraction of tumor cells. There were no significant differences in clinical findings or overall patient survival between T-or NK-cell types of ENKTL, although those with a T-cell type tended to show a better prognosis for those with localized nasal lymphomas. Univariate and multivariate analysis showed that a non-nasal ENKTL, age 460 years, high level of lactate dehydrogenase, bone marrow involvement, and the absence of radiotherapy were independent prognostic factors.</P>

Comparison of 90‐day case‐fatality after ischemic stroke between two different stroke outcome registries using propensity score matching analysis

Yu, K‐,H.,Hong, K‐,S.,Lee, B‐,C.,Oh, M‐,S.,Cho, Y‐,J.,Koo, J‐,S.,Park, J‐,M.,Bae, H‐,J.,Han, M‐,K.,Ju, Y‐,S.,Kang, D‐,W.,Appelros, P. Blackwell Publishing Ltd 2011 Acta neurologica Scandinavica Vol.123 No.5

<P>Yu K‐H, Hong K‐S, Lee B‐C, Oh M‐S, Cho Y‐J, Koo J‐S, Park J‐M, Bae H‐J, Han M‐K, Ju Y‐S, Kang D‐W, Appelros P, Norrving B, Terent A. Comparison of 90‐day case‐fatality after ischemic stroke between two different stroke outcome registries using propensity score matching analysis. 
Acta Neurol Scand: 2011: 123: 325–331. 
© 2010 John Wiley & Sons A/S.</P><P><B>Background – </B> It has not been clarified whether the disparity in ischemic stroke outcome between populations is caused by ethnic and geographic differences or by variations in case mix. Propensity score matching (PSM) analysis can overcome some analytical problems but is rarely used in stroke outcome research. This study was to compare the ischemic stroke case‐fatality between two PSM cohorts of Sweden and Korea.</P><P><B>Methods – </B> Prognostic variables related to baseline characteristics and stroke care were included in our PSM model. Then, we selected 7675 Swedish and 1220 Korean patients with ischemic stroke from each stroke registers and performed one‐to‐one matching based on propensity scores of each patient.</P><P><B>Results – </B> After PSM, all measured variables were well balanced in 1163 matched subjects, and the 90‐day case‐fatality was identical 6.2% (HR 0.997, 95%CI 0.905–1.099) in Sweden and Korea.</P><P><B>Conclusions – </B> No difference is found in the 90‐day case‐fatality in propensity score‐matched Swedish and Korean patients with ischemic stroke.</P>

부산시 기장군 연안의 해조상 및 군집 특성

최창근,최인영,홍용기,Choi, Chang-Geun,Chowdhury, M.T.H.,Choi, In-Young,Hong, Yong-Ki 한국해양학회 2010 바다 Vol.15 No.3

기장 연안 해조류의 해조상 및 군집 구조를 분석하기 위해서 2006년 8월과 2009년 8월에 연구를 수행하였다. 해조류는 녹조류 6종, 갈조류 10종, 홍조류 38종 등 총 54종의 서식이 확인되었다. 이 중 35종의 해조류가 각각의 조사 시기에 공통적으로 출현하였다. 평균 현존량은 2006년에 $616.0\;g\;m^{-2}$에서 $1,462.4\;g\;m^{-2}$였고, 2009년에는 $354.8\;g\;m^{-2}$에서 $965.6\;g\;m^{-2}$이었다. 현존량이 가장 높은 지역은 문동이었고, 가장 낮은 곳은 서암(2006년)과 동백(2009년)이었다. 출현 해조류를 6개의 기능형으로 구분하면, 성긴분지형이 58.7%와 58.1%, 다육질형이 10.9%와 11.6%, 사상형이 13.0%와 9.3%, 각상형이 6.5%와 9.3%, 엽상형이 6.5%와 7.0%, 유절산호말형이 4.3%와 4.7%로 나타났다. 생태지수인 R/P, C/P, (R+C)/P 값은 2006년에 4.00, 0.75, 4.75와 2009년에 5.17, 1.00, 6.17로 나타났다. 2006년과 2009년의 자료를 분석했을 때, 기장 연안의 해조류 출현 종 수와 현존량은 유사하였다. 이러한 자료로 볼 때, 기장 연안에서 시행된 소규모 공사 전, 후인 2006년과 2009년의 해조류 종 다양성에는 변화가 나타나지 않은 것으로 분석되었다. Marine algal flora and community structure were investigated at four sites in Kijang area on the southern east coast of Korea in August 2006 and August 2009, respectively. A total of 54 seaweeds including 6 green, 10 brown, 38 red were collected and identified. Of 54 seaweeds, 35 species were found throughout the survey period. Mean biomass in wet weight was from $616.0\;g\;m^{-2}$ to $1,462.4\;g\;m^{-2}$2 in 2006, and $354.8\;g\;m^{-2}$ to $965.6\;g\;m^{-2}$ in 2009. Maximum biomass was recorded at Mundong site, and minimum was recorded at Seoam (2006) and Dongbaek (2009) sites. The flora investigated (2006, 2009) could be classified into six functional groups such as coarsely branched form (58.7%, 58.1%), thick leathery form (10.9%, 11.6%), filamentous form (13.0%, 9.3%), crustose form (6.5%, 9.3%), sheet form (6.5%, 7.0%) and jointed calcareous form (4.3%, 4.7%) during survey period. The R/P, C/P and (R+C)/P values reflecting flora characteristics were 4.00, 0.75 and 4.75 at 2006, and 5.17, 1.00 and 6.17 at 2009, respectively. Therefore, the number of marine algae species and biomass in Kijang area were similar when they were comparing with the previous data. It suggest that any changes of seaweed diversity have not been observed in Kijang coastal area before and after the anthropogenic construction between 2006 and 2009.

Characterization of Alcohol Dehydrogenase Encoded by Zymomonas mobilis Gene Cloned in Escherichia coli

Shin, Byung Sik,Yoon, Ki Hong,Park, M. Y. 한국산업미생물학회 1990 한국미생물·생명공학회지 Vol.18 No.3

Zynomonas mobilis의 알코올 탈수소 효소 유전자가 클로닝된 대장균 형질전환체의 세포 추출물로부터 알코올 탈수소 효소를 분리정제하였다. 형질전환된 Escherichia coli(pADS93)가 생산하는 Z. mobilis 유전자 유래의 알코올 탈수소 효소는 분자량이 40,000인 동일한 4개의 subunits로 구성된 tetramer임이 밝혀졌으며 이것은 Z. mobilis 세포 추출물로부터 분리한 알코올 탈수소 효소와 동일하였다. 이 효소의 정반응(ethanol 산화)은 pH의 영향을 많이 받으며 최적의 pH는 10.0이었고 역반응(acetaldehyde 환원)에서는 최적의 pH가 7.5∼8.5이었지만 pH에 따라 크게 영향을 받지는 않았다. 한편 기질인 ethanol과 NAD에 대한 K_m값은 각각 1.2×10 exp(-1)M과 5.1×10 exp(-5)M로 나타났으며 acetaldehyde에 대한 K_m값은 ethanol에 대한 K_m값보다 매우 낮았다. The structural gene(zadhⅡ) encoding an alcohol dehydrogenase Ⅱ from Zymomonas mobilis was cloned into Escherichia coli in our laboratory(Yoon et al., 1989. Kor. J. Microbiol. Biotechnol.). From E. coli(pADS93) carrying the zadhⅡ gene, the Z. mobilis alcochol dehydrogenase Ⅱ(ZADH-Ⅱ) was purified by sonication, (NH_4)_2SO_4 fractionation, and chromatography. The ZADH-Ⅱ enzyme produced by Z. mobilis cell was also purified to compare to the enzyme produced by E. coli(pADS93). The purified enzyme from cell extract of E. coli(pADS93) was identified to be a tetramer being composed of four identical subunits having molecular weight of 40,000 dalton like that of Z. mobilis. The pH optimum for the reaction oxidizing ethanol to acetaldehyde was 10.0 while the optimum for the reverse reaction was 7.5-8.5. The apparent K_m values for ethanol and NAD^+ were 1.2×10 exp(-1) M and 5.1×10 exp(-5) M, respectively. In addition, it was found that the K_m value for acetaldehyde was very lower than that for ethanol.

Thermal Dissociation and Conformational Lock of Superoxide Dismutase

Hong, J.,Moosavi-Movahedi, A.A.,Ghourchian, H.,Amani, M.,Amanlou, M.,Chilaka, F.C. Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.5

The kinetics of thermal dissociation of superoxide dismutase (SOD) was studied in 0.05 M Tris-HCl buffer at pH 7.4 containing $10^{-4}\;M$ EDTA. The number of conformational locks and contact areas and amino acid residues of dimers of SOD were obtained by kinetic analysis and biochemical calculation. The cleavage bonds between dimers of SOD during thermal dissociation and type of interactions between specific amino acid residues were also simulated. Two identical contact areas between two subunits were identified. Cleavage of these contact areas resulted in dissociation of the subunits, with destruction of the active centers, and thus, lost of activity. It is suggested that the contact areas interact with active centers by conformational changes involving secondary structural elements.

Mössbauer 분광법에 의한 원자로 용기재료의 비파괴적 중성자 조사평가에 대한 연구

정명모(M. M. Jung),장기상(K. S. Jang),유근배(K. B. Yoo),김길무(G. M. Kim),윤인섭(I. S. Yoon),홍치유(C. Y. Hong) 한국비파괴검사학회 2000 한국비파괴검사학회지 Vol.20 No.3

Ti-Ni 형상기억합금의 반복변형에 의한 성능렬화현상

홍종휘,신명철,오명훈,지광구,장우양 대한금속재료학회(대한금속학회) 1989 대한금속·재료학회지 Vol.27 No.1

The effect of deformation on the transformation behavior in Ti-Ni alloy was studied. Coil spring of Ti-Ni alloy used as element of tilt plate type heat engine, was cyclically expanded and contracted by temperature difference. The Results obtained in this study are as follows. 1. By the cyclic deformation, M_s and A_f decreased with increasing the number of cycles, however, M_s remained constant. The cycling effect was caused by the generation of dislocation during cyclic deformation 2. The cycling effect (decrease of M_s and A_f) increased with the increase of the heat treatment temperature and deformation strain. When the cyclic deformation was conducted in the temperature range of B2↔R-phase, transformation temperature changed little. 3. That unrecovered strain appeared to be proportional to the amount of decrease of M_s and A_f confirmed that cycling effect was caused by introduction of dislocation during deformation.

Comparative study between N-body and Fokker–Planck simulations for rotating star clusters – II. Two-component models

Hong, J.,Kim, E.,Lee, H. M.,Spurzem, R. Oxford University Press 2013 Monthly notices of the Royal Astronomical Society Vol.430 No.4

<P>To understand the effects of the initial rotation on the evolution of the tidally limited clusters with mass spectrum, we have performed N-body simulations of the clusters with different initial rotations and compared the results with those of the Fokker-Planck (FP) simulations. We confirmed that the cluster evolution is accelerated by not only the initial rotation but also the mass spectrum. For the slowly rotating models, the time evolutions of mass, energy and angular momentum show good agreements between N-body and FP simulations. On the other hand, for the rapidly rotating models, there are significant differences between these two approaches at the early stage of the evolutions because of the development of bar instability in N-body simulations. The shape of the cluster for N-body simulations becomes tri-axial or even prolate, which cannot be produced by the two-dimensional FP simulations. The total angular momentum and the total mass of the cluster decrease rapidly while bar-like structure persists. After the rotational energy becomes smaller than the critical value for the bar instability, the shape of the cluster becomes nearly axisymmetric again, and follows the evolutionary track predicted by the FP equation. We have confirmed again that the energy equipartition is not completely achieved when M-2/M-1(m(2)/m(1))(3/2) > 0.16. By examining the angular momentum at each mass component, we found that the exchange of angular momentum between different mass components occurs, similar to the energy exchange leading to the equipartition.</P>

Phenological growth stages of <i>Brachypodium distachyon</i>: codification and description

HONG, S‐,Y,PARK, J‐,H,CHO, S‐,H,YANG, M‐,S,PARK, C‐,M Blackwell Publishing Ltd 2011 Weed research Vol.51 No.6

<P>H<SMALL>ong</SMALL> S‐Y, P<SMALL>ark</SMALL> J‐H, C<SMALL>ho</SMALL> S‐H, Y<SMALL>ang</SMALL> M‐S & P<SMALL>ark</SMALL> C‐M (2011). Phenological growth stages of <I>Brachypodium distachyon</I>: codification and description. <I>Weed Research</I><B>51</B>, 612–620.</P><P><B>Summary</B></P><P><I>Brachypodium distachyon</I> is being used as a new model monocotyledon for grass crop research and bioenergy grass biotechnology. With the recent completion of the full genome sequencing project, one of the next major challenges is to determine the functional activities of many genes. In an attempt to aid the rapid determination of gene function in crop plants, a high throughput phenotypic analysis system has been established through phenological analyses of a series of defined growth stages. The codification follows the BBCH (Biologische Bundesantalt, Bundessortenamt and CHemische Industrie) scale, a numerical system that differentiates between principal and secondary growth stages from seed germination to flower head emergence and seed ripening. We present here analyses of the phenological development stages of <I>B.?distachyon</I> to illustrate a methodological framework that can be used to identify and interpret phenotypic differences derived from genetic variations and environmental stress responses in this plant species. This scale may greatly contribute to the <I>B.?distachyon</I> research in the future, particularly to functional determination of genes that mediate biomass productivity. It may also serve as a suitable tool for defining the growth stages of other grass species.</P>