Evolution of Packing Parameters in the Structural Changes of Silica Mesoporous Crystals: Cage-Type, 2D Cylindrical, Bicontinuous Diamond and Gyroid, and Lamellar

Han, Lu,Miyasaka, Keiichi,Terasaki, Osamu,Che, Shunai American Chemical Society 2011 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.133 No.30

<P>Cage-type, two-dimensional (2D) cylindrical hexagonal (<B>C</B>), bicontinuous diamond (<B>D</B>), bicontinuous gyroid (<B>G</B>), and one-dimensional (1D) lamellar (<B>L</B>) structures of silica mesoporous crystals (SMCs) were obtained by using the anionic surfactant <I>N</I>-stearoyl-<SMALL>l</SMALL>-glutamic acid (C<SUB>18</SUB>GluA) as a template in the presence of the nonionic surfactant C<SUB>16</SUB>(EO)<SUB>10</SUB> (Brij-56). The mesostructures were controlled by the organic/inorganic interface curvature change induced by Brij-56. A synthesis-field diagram showed that the mesostructure changed in the sequence cage-type → <B>C</B> → intergrowth of <B>C</B> and <B>D</B> → intergrowth of <B>C</B> and <B>G</B> → <B>D</B> → <B>G</B> → <B>L</B> with increase of the amount of Brij-56. Mixed micelles were formed by the anionic and nonionic surfactants, the packing parameter <I>g</I> of which increased with increasing the addition amount of nonionic surfactant and the reaction temperature. The local <I>g</I> parameter was obtained from electron crystallography reconstruction results by calculating mean curvatures and Gaussian curvatures from the equi-electrostatic potential surface. The intergrowth of <B>C</B> and <B>D</B> and two kinds of intergrowth of <B>C</B> and <B>G</B> are also discussed.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/2011/jacsat.2011.133.issue-30/ja200683t/production/images/medium/ja-2011-00683t_0011.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ja200683t'>ACS Electronic Supporting Info</A></P>

Methylation Status of Transcriptional Modulatory Genes Associated with Colorectal Cancer in Northeast China

Han-Lu Gao,Xuan Wang,Hong-Ru Sun,Jun-De Zhou,Shang-Qun Lin,Yu-Hang Xing,Lin Zhu,Hai-Bo Zhou,Ya-Shuang Zhao,Qiang Chi,Yu-Peng Liu 거트앤리버 소화기연관학회협의회 2018 Gut and Liver Vol.12 No.2

Background/Aims: Methylation status plays a causal role in carcinogenesis in targeted tissues. However, the relationship between the DNA methylation status of multiple genes in blood leukocytes and colorectal cancer (CRC) susceptibility as well as interactions between dietary factors and CRC risks are unclear. Methods: We performed a case-control study with 466 CRC patients and 507 cancer-free controls to investigate the association among the methylation status of individual genes, multiple CpG site methylation (MCSM), multiple CpG site heterogeneous methylation and CRC susceptibility. Peripheral blood DNA methylation levels were detected by performing methylation-sensitive high-resolution melting. Results: Total heterogeneous methylation of CA10 and WT1 conferred a significantly higher risk of CRC (adjusted odds ratio [ORadjusted], 5.445; 95% confidence interval [CI], 3.075 to 9.643; ORadjusted, 1.831; 95% CI, 1.100 to 3.047; respectively). Subjects with high-level MCSM (MCSM-H) status demonstrated a higher risk of CRC (ORadjusted, 4.318; 95% CI, 1.529 to 12.197). Additionally, interactions between the high-level intake of fruit and CRH, WT1, and MCSM on CRC were statistically significant. Conclusions: The gene methylation status of blood leukocytes may be associated with CRC risk. MCSM-H of blood leukocytes was associated with CRC, especially in younger people. Some dietary factors may affect hypermethylation status and influence susceptibility to CRC.

Fisetin inhibits the activities of cyclin-dependent kinases leading to cell cycle arrest in HT-29 human colon cancer cells.

Lu, Xianghua,Jung, Jae in,Cho, Han Jin,Lim, Do Young,Lee, Hyun Sook,Chun, Hyang Sook,Kwon, Dae Young,Park, Jung Han Yoon Wistar Institute of Anatomy and Biology 2005 The Journal of nutrition Vol.135 No.12

<P>Fisetin, a natural flavonol present in edible vegetables, fruits, and wine, was reported to exert anticarcinogenic effects. The objective of the current study was to examine the effect of fisetin on the cell cycle progression of the human colon cancer cell line HT-29. HT-29 cells were cultured in serum-free medium with 0, 20, 40, or 60 micromol/L fisetin. Fisetin dose dependently inhibited both cell growth and DNA synthesis (P < 0.05), with a 79 +/- 1% decrease in cell number observed 72 h after the addition of 60 micromol/L fisetin. Perturbed cell cycle progression from the G(1) to S phase was observed at 8 h with 60 micromol/L fisetin treatment, whereas a G(2)/M phase arrest was observed after 24 h (P < 0.05). The phosphorylation state of the retinoblastoma proteins shifted from hyperphosphorylated to hypophosphorylated in cells treated with 40 micromol/L fisetin. (P < 0.05). Fisetin decreased the activities of cyclin-dependent kinases (CDK)2 and CDK4; these effects were likely attributable to decreases in the levels of cyclin E and D1 and an increase in p21(CIP1/WAF1) levels (P < 0.05). However, fisetin also inhibited CDk4 activity in a cell-free system (P < 0.05), indicating that it may directly inhibit CDk4 activity. The protein levels of cell division cycles (CDC)2 and CDC25C and the activity of CDC2 were also decreased in fisetin-treated cells (P < 0.05). These results indicate that inhibition of cell cycle progression in HT-29 cells after treatment with fisetin can be explained, at least in part, by modification of CDK activities.</P>

Semi-supervised Multi-view Manifold Discriminant Intact Space Learning

( Lu Han ),( Fei Wu ),( Xiao-yuan Jing ) 한국인터넷정보학회 2018 KSII Transactions on Internet and Information Syst Vol.12 No.9

Semi-supervised multi-view latent space learning is gaining considerable popularity recently in many machine learning applications due to the high cost and difficulty to obtain the large amount of label information of data. Although some semi-supervised multi-view latent space learning methods have been presented, there is still much space for improvement: 1) How to learn latent discriminant intact feature representations by employing data of multiple views; 2) How to exploit the manifold structure of both labeled and unlabeled point in the learned latent intact space effectively. To address the above issues, we propose an approach called semi-supervised multi-view manifold discriminant intact space learning (SM2DIS) for image classification in this paper. SM2DIS aims to seek a manifold discriminant intact space for data of different views by making use of both the discriminant information of labeled data and the manifold structure of both labeled and unlabeled data. Experimental results on MNIST, COIL-20, Multi-PIE, and Caltech-101 databases demonstrate the effectiveness and robustness of our proposed approach.

Expression of GA733-Fc Fusion Protein as a Vaccine Candidate for Colorectal Cancer in Transgenic Plants

Lu, Zhe,Lee, Kyung-Jin,Shao, Yingxue,Lee, Jeong-Hwan,So, Yangkang,Choo, Young-Kug,Oh, Doo-Byoung,Hwang, Kyung-A,Oh, Seung Han,Han, Yeon Soo,Ko, Kisung Hindawi Publishing Corporation 2012 Journal of biomedicine & biotechnology Vol.2012 No.-

<P>The tumor-associated antigen GA733 is a cell-surface glycoprotein highly expressed in colorectal carcinomas. In this study, 3 recombinant genes were constructed as follows: GA733 tagged to the ER retention sequence KDEL (GA733K), GA733 fused to the immunoglobulin Fc fragment (GA733-Fc), and GA733-Fc fused to the ER retention sequence (GA733-FcK). <I>Agrobacterium</I>-mediated transformation was used to generate transgenic plants expressing recombinant genes. The presence of transgenes was confirmed by genomic PCR. Western blot, confocal immunofluorescence, and sandwich ELISA showed the expression of recombinant proteins. The stability, flexibility, and bioactivity of recombinant proteins were analyzed and demonstrated through N-glycosylation analysis, animal trials, and sera ELISA. Our results suggest that the KDEL retained proteins in ER with oligomannose glycan structure and enhanced protein accumulation level. The sera of mice immunized with GA733-FcK purified from plants contained immunoglobulins which were at least as efficient as the mammalian-derived GA733-Fc at recognizing human colorectal cancer cell lines. Thus, a plant system can be used to express the KDEL fusion protein with oligomannose glycosylation, and this protein induces an immune response which is comparable to non-KDEL-tagged, mammalian-derived proteins.</P>

<i>Plasmodium ovale curtisi</i> and <i>Plasmodium ovale wallikeri</i> in Chinese travelers: Prevalence of novel genotypes of circumsporozoite protein in the African continent

Lu, Feng,Ahmed, Md Atique,Xu, Simin,Xu, Sui,Han, Jin-Hee,Liu, Qianyan,Chen, Jing,Zhu, Guoding,Zhou, Huayun,Cao, Jun,Han, Eun-Taek ELSEVIER 2019 INFECTION GENETICS AND EVOLUTION Vol.70 No.-

<P><B>Abstract</B></P> <P>Imported malaria due to <I>Plasmodium ovale curtisi</I> and <I>P</I>. <I>ovale wallikeri</I> infections from African countries has increased recently (2011–2014) in Chinese travelers. We report novel genotypes, their prevalence and the predominant haplotypes of <I>P</I>. <I>ovale curtisi</I> and <I>P</I>. <I>ovale wallikeri</I> circumsporozoite protein (CSP) from 20 African countries in Chinese travelers. These genotypes should be considered while designing a CSP-based vaccine against <I>P</I>. <I>ovale</I> malaria.</P> <P><B>Highlights</B></P> <P> <UL> <LI> This is the first to report the <I>csp</I> genotypes and their heterogeneity for <I>P. ovale curtisi</I> and <I>P. ovale wallikeri</I> parasites from African countries. </LI> <LI> Ten <I>csp</I> genotypes of <I>P</I>. <I>ovale curtisi</I> and 13 <I>csp</I> genotypes of <I>P</I>. <I>ovale wallikeri</I> based on the arrangement of central repeat motifs were found. </LI> <LI> Fifteen <I>csp</I> haplotypes of <I>P</I>. <I>ovale curtisi</I> and 10 <I>csp</I> haplotypes of <I>P</I>. <I>ovale wallikeri</I> on central repeat motifs were identified. </LI> <LI> These genotypes should be considered while designing a CSP-based vaccine against <I>P</I>. <I>ovale</I> malaria. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Testing Cost Inefficiency under Free Entry in the Real Estate Brokerage Industry

Lu Han,Seung-Hyun Hong 한국계량경제학회 2009 한국계량경제학회 학술대회 논문집 Vol.2009 No.2

In this article we provide an empirical framework to study entry and cost inefficiency in the real estate brokerage industry. Building upon recent empirical work on games of incomplete information, we develop an equilibrium model that incorporates unique features of this industry. Using individual-level data on entry and revenues, we estimate our entry model to recover the cost function. Based on estimated costs, we directly test for cost inefficiency due to free entry and find evidence for a loss of economies of scale and wasteful non-price competition. We further use the estimated model to evaluate welfare implications of prohibiting rebates on commissions. We find that rebate bans are welfare-reducing, not only because they discourage price competition, but also because they encourage excessive entry. In particular, removing these rebate bans would decrease the equilibrium number of realtors by 7.2% and reduce total variable costs by 4.1%.

Carboxylic group functionalized ordered mesoporous silicas

Han, Lu,Terasaki, Osamu,Che, Shunai Royal Society of Chemistry 2011 Journal of materials chemistry Vol.21 No.30

<P>Many research efforts have focused on the synthesis of organic and inorganic hybrid ordered mesoporous silicas (MSs) with functionalization of the exterior and/or interior surfaces aiming for applications in separation, adsorption, catalysis, drug delivery, and nanotechnology. Among the organic groups, the carboxylic group is a particularly useful reactive group for many applications. This article provides a brief overview of the carboxylic group functionalized MSs and the recent progress in synthetic strategies and applications have been reviewed.</P> <P>Graphic Abstract</P><P>This article highlights the recent progress in the synthetic strategies and applications of ordered mesoporous silicas functionalized with carboxylic groups. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c1jm10561b'> </P>

태블릿 PC의 GUI 디자인 연구

루한이(Han-Yi Lu),서한석(Han-Sok Seo) 한국콘텐츠학회 2017 한국콘텐츠학회논문지 Vol.17 No.7

태블릿 PC 사용자 수가 날로 증가되고 있는 배경 하에, 온라인 쇼핑 방면에 관한 애플리케이션도 상응하게 빠른 발전을 가져 왔다. 하지만 실제 상황으로 사용자 사용능력의 차이는 쇼핑 애플리케이션이 사용자 수요의 만족에 영향을 준다. 하여, 쇼핑 애플리케이션은 GUI 디자인에서 사용자 사용성을 제고하는 방면에 있어 개선이 필요하고, 이로써 각종 유형의 사용자 사용수요를 만족해야 한다. 따라서 본 논문은 태블릿 PC용 애플리케이션의 GUI 디자인 상관연구를 진행하면서, 정보전달에 대한 시각화 그래픽요소를 충분히 고려하고, 이제 중국시장에 순위 5개 쇼핑 애플리케이션을 대상으로 아이콘, 레이블과 컬러 3가지 GUI 디자인 시각적 구성요소에 대한 내용을 비교 분석하였다. 또한, 태블릿 PC 쇼핑 애플리케이션의 중국 사용자를 대상으로 설문조사를 진행하고, 조사결과를 기초로 하여 기능성과 GUI 디자인을 조화롭게 반영할 수 있는 쇼핑 애플리케이션의 GUI 디자인 제안을 제출하였다. 본 연구 결과를 통해 아이콘, 레이블, 컬러 3가지 시각적인 GUI 구성요소가 사용자에게 사용성에 영향을 미친다는 것을 알 수 있었다. 또한, 본 연구가 쇼핑 앱 GUI 디자인 분야에 참고할 만한 자료로 기대된다. In the context of the number of Tablet PC users which increases rapidly, online shopping application has also been a corresponding rapid development. However, as a practical matter, the differences among the consumers ability will affect the purchase of application for the realization of user needs. Therefore, shopping application in the GUI design to improve consumers use needs to be improved to meet the demands of different users. In view of this problem, this paper has carried on the related research on the GUI design of Tablet PC application, and fully considers the visualization image elements of information transmission, then analyzed the top five shopping application in Chinese market via three visual elements - icon, label and color. In addition, it conducted a questionnaire which based on the object of Tablet PC shopping application Chinese users. Consequently, what obtained is, based on the survey findings, the GUI design proposal which reasonable reflects the shopping application with functionality and designability. Through the research results of this paper, we can get the conclusion that the three kinds of visual GUI elements-icon, label and color- have the influence on the users usability. In addition, this study can be expected as a reference in the shopping software GUI design.

고로슬래그 미분말 기반 무시멘트 모르타르의 석고 및 소각장애시에 의한 성능향상 검토

여량량(Lu, Liang-Liang),박재용(Park, Jae-Yong),황금광(Huang,Jin-Guang),정상운(Jung,Sang-woon),한민철(Han, Min-Cheol),한천구(Han,Cheon-Goo) 한국콘크리트학회 2014 한국콘크리트학회 학술대회 논문집 Vol.2014 No.5