Effects of short-term soil tillage management on activity and community structure of denitrifiers under double-cropping rice field

Haiming Tang,Chao Li,Kaikai Cheng,Lihong Shi,Li Wen,Xiaoping Xiao,Yilan Xu,Weiyan Li,Ke Wang 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.11

Soil physical and chemical characteristics, soil potential denitrification rates (PDR), community composition and nirK-, nirS- and nosZ-encoding denitrifiers were studied by using MiSeq sequencing, quantitative polymerase chain reaction (qPCR), and terminal restriction fragment polymorphism (T-RFLP) technologies base on short-term (5-year) tillage field experiment. The experiment included four tillage treatments: conventional tillage with crop residue incorporation (CT), rotary tillage with crop residue incorporation (RT), no-tillage with crop residue retention (NT), and rotary tillage with crop residue removed as control (RTO). The results indicated that soil organic carbon, total nitrogen and NH4 +-N contents were increased with CT, RT and NT treatments. Compared with RTO treatment, the copies number of nirK, nirS and nosZ in paddy soil with CT, RT and NT treatments were significantly increased. The principal coordinate analysis indicated that tillage management and crop residue returning management were the most and the second important factors for the change of denitrifying bacteria community, respectively. Meanwhile, this study indicated that activity and community composition of denitrifiers with CT, RT and NT treatments were increased, compared with RTO treatment. This result showed that nirK, nirS and nosZ-type denitrifiers communities in crop residue applied soil had higher species diversity compared with crop residue removed soil, and denitrifying bacteria community composition were dominated by Gammaproteobacteria, Deltaproteobacteria, and Betaproteobacteria. Therefore, it is a beneficial practice to increase soil PDR level, abundance and community composition of nitrogen-functional soil microorganism by combined application of tillage with crop residue management.

Device-to-Device Communication Underlaying Cellular Networks: Connection Establishment and Interference Avoidance

( Shaoyi Xu ),( Haiming Wang ),( Tao Chen ),( Tao Peng ),( Kyung Sup Kwak ) 한국인터넷정보학회 2012 KSII Transactions on Internet and Information Syst Vol.6 No.1

It is expected that device-to-device (D2D) communication is allowed to underlay future cellular networks such as IMT-Advanced for spectrum efficiency. This article studies the mechanisms of D2D communication and interference avoidance when the D2D subsystem reuses uplink resources and downlink spectrums with a cellular system, respectively. We firstly propose an effective scheme to establish and maintain D2D communication. Moreover, a novel method to deal with the resource allocation and interference avoidance issues by utilizing the network peculiarity of a hybrid network to share the uplink resource is proposed. Most research focuses on reusing the uplink spectrums, but how to share the downlink frequency bands is seldom addressed. To share the downlink spectrums and avoid the interference to the primary cellular devices, a labeled time slots based mechanism is proposed. Implementation details are described in a real cellular system and simulation results prove that satisfying performance can be achieved by using the proposed mechanisms.

An Anti-Overvoltage Strategy for Electrolytic Capacitorless IPMSM Drives Using Power Controller

Dawei Ding,Haiming Hu,Gaolin Wang,Guoqiang Zhang,Dianguo Xu,Frede Blaabjerg 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

Three-phase input diode rectifier interior permanent magnet synchronous motor (IPMSM) drives with small-volume film capacitors have many merits, such as high reliability, low cost and high power density. However, in regenerative braking, the DC-link voltage becomes harder to be controlled than the traditional topology due to the application of slim film capacitors. In this paper, an anti-overvoltage strategy is proposed by controlling the electrical power of the motor directly. The q-axis current boundary is determined by the proposed power controller. Compared with the conventional method of voltage controller, the proposed method is more suitable for electrolytic capacitorless drives.

Effects of dietary fiber levels on cecal microbiota composition in geese

Li, Yanpin,Yang, Haiming,Xu, Lei,Wang, Zhiyue,Zhao, Yue,Chen, Xiaoshuai Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.8

Objective: This study shows the effects of dietary fiber levels on cecal microbiota composition in geese at day 70 according to pyrosequencing of the 16S ribosomal RNA gene. Methods: A total of 468 1-day-old healthy male Yangzhou goslings with similar body weight were randomly divided into 3 groups with 6 replicates per group and 26 geese per replicate. Geese were fed diets with fiber levels of 2.5% (low fiber level diet, Group I) and 6.1% (Group III) during days 1-70, respectively, or 4.3% for days 1-28 and 6.1% for days 29-70 (Group II). Results: Low fiber level diet decreased body weight, average daily gain during, increased lower feed conversation rate of geese during day 1 to 70 (p<0.05). Low fiber level diet decreased the total operational taxonomic units, Chao1 index and Shannon index, whereas increased the Simpson index of cecal microbiota in geese at day 70. Low fiber level diet decreased the relative abundance of Bacteroidetes, Firmicutes, Bacteroides, and Paraprevotella in cecum of geese at day 70. The similarity of cecal microbiota between low fiber level diet group and other groups was smaller. Conclusion: This study indicates that the low fiber level diet decreased diversity of microbiota, and relative abundance of some beneficial microbiota in cecum of geese at day 70, implying that the low fiber level diet has negative influence on performance by altering the diversity and population of cecal microbiota in geese.

Rapid fabrication of NiFe(OH)x/Fe0.2Co-Se complexes for oxygen evolution reaction electrocatalysis

Kang Gao,Miao Xu,Chuankun Zhang,Haiming Huang,Dongming Cai,Minglei Cao,Jun Wu,Chengrui Wu,Yongjin Hu,Rui Tong 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.124 No.-

Exploiting the inexpensive and efficient oxygen evolution reaction (OER) electrocatalysts is crucial forimproving the efficiency of electrocatalytic water splitting (EWS), since the slow 4-electron OER processis a rate-limiting step in EWS. Herein, we prepared the NiFe(OH)x/Fe0.2Co-Se complexes on carbon paper(CP) via an easy two-step electrochemical deposition strategy. By varying the doping amount of Fe andthe deposition time of Fe0.2Co-Se, the OER property of NiFe(OH)x/Fe0.2Co-Se/CP was optimized to the best:only requiring low overpotentials of 243 and 277 mV at the current density of 20 and 100 mA/cm2 (j20and j100) and a small Tafel slope of 36.8 mV/dec, along with a terrific long-term durability in 1 M KOH. According to the experimental results, the rich exposed active sites, improved intrinsic site activityand enhanced electrical conductivity lead to the superior OER performance. Our work will provide someinspiration for designing other metal selenide/hydroxide heterostructures for other energy conversion orstorage applications.

Effects of lycopene on abdominal fat deposition, serum lipids levels and hepatic lipid metabolism-related enzymes in broiler chickens

Wan Xiaoli,Yang Zhengfeng,Ji Haoran,Li Ning,Yang Zhi,Xu Lei,Yang Haiming,Wang Zhiyue 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.3

Objective: The present study was conducted to investigate the effects of lycopene on growth performance, abdominal fat deposition, serum lipids levels, activities of hepatic lipid metabolism related enzymes and genes expression in broiler chickens. Methods: A total of 256 healthy one-day-old male Arbor Acres broiler chicks were randomly divided into four groups with eight replicates of eight birds each. Birds were fed basal diet supplemented with 0 (control), 100, 200, and 400 mg/kg lycopene, respectively. Results: Dietary 100 mg/kg lycopene increased the body weight at 21 day of age compared to the control group (p<0.05). Compared to the basal diet, broilers fed diet with 100 mg/kg lycopene had decreased abdominal fat weight, and broilers fed diet with 100 and 200 mg/kg lycopene had decreased abdominal fat percentage (p<0.05). Compared to control, diets with 100, 200, and 400 mg/kg lycopene reduced the levels of total triglyceride and total cholesterol in serum, and diets with 100 and 200 mg/kg lycopene reduced the level of serum low density lipoprotein cholesterol (p<0.05). The activity of fatty acid synthase (FAS) in 400 mg/kg lycopene treated broilers and the activity of acetyl-CoA carboxylase (ACC) in 100, 200, and 400 mg/kg lycopene treated broilers were lower than those fed basal diet (p<0.05). Lycopene increased the mRNA abundance of adenosine monophosphate activated protein kinase α (AMPK-α), whereas decreased the mRNA abundance of sterol regulatory elementbinding protein 1, FAS, and ACC compared to the control group (p<0.05). Conclusion: Dietary lycopene supplementation can alleviate abdominal fat deposition and decrease serum lipids levels, possibly through activating the AMPK signaling pathway, thereby regulating lipid metabolism such as lipogenesis. Therefore, lycopene or lycopenerich plant materials might be added to poultry feed to regulate lipid metabolism. Objective: The present study was conducted to investigate the effects of lycopene on growth performance, abdominal fat deposition, serum lipids levels, activities of hepatic lipid metabolism related enzymes and genes expression in broiler chickens.Methods: A total of 256 healthy one-day-old male Arbor Acres broiler chicks were randomly divided into four groups with eight replicates of eight birds each. Birds were fed basal diet supplemented with 0 (control), 100, 200, and 400 mg/kg lycopene, respectively.Results: Dietary 100 mg/kg lycopene increased the body weight at 21 day of age compared to the control group (p<0.05). Compared to the basal diet, broilers fed diet with 100 mg/kg lycopene had decreased abdominal fat weight, and broilers fed diet with 100 and 200 mg/kg lycopene had decreased abdominal fat percentage (p<0.05). Compared to control, diets with 100, 200, and 400 mg/kg lycopene reduced the levels of total triglyceride and total cholesterol in serum, and diets with 100 and 200 mg/kg lycopene reduced the level of serum low density lipoprotein cholesterol (p<0.05). The activity of fatty acid synthase (FAS) in 400 mg/kg lycopene treated broilers and the activity of acetyl-CoA carboxylase (ACC) in 100, 200, and 400 mg/kg lycopene treated broilers were lower than those fed basal diet (p<0.05). Lycopene increased the mRNA abundance of adenosine monophosphate activated protein kinase α (AMPK-α), whereas decreased the mRNA abundance of sterol regulatory elementbinding protein 1, FAS, and ACC compared to the control group (p<0.05).Conclusion: Dietary lycopene supplementation can alleviate abdominal fat deposition and decrease serum lipids levels, possibly through activating the AMPK signaling pathway, thereby regulating lipid metabolism such as lipogenesis. Therefore, lycopene or lycopenerich plant materials might be added to poultry feed to regulate lipid metabolism.

Enhancement of Titers for Vectors Encoding miRNA Adapted shRNAs (shRNA<SUP>miR</SUP>s) Associated with Increased Viral Genomic Transcripts via DROSHA Knockout in Producer Cells

Hee Ho PARK,Robinson TRIBOULET,Martin BENTLER,Swaroopa GUDA,Peng DU,Haiming XU,Richard I. GREGORY,Christian BRENDEL,David A. WILLIAMS 한국생물공학회 2017 한국생물공학회 학술대회 Vol.2017 No.10

DROSHA Knockout Producer Cells Toward High Viral Titers for Vectors Encoding miRNA- dapted shRNAs (shRNA<SUP>miR</SUP>s)

Hee Ho PARK,Robinson TRIBOULET,Martin BENTLER,Swaroopa GUDA,Peng DU,Haiming XU,Richard I. GREGORY,Christian BRENDEL,David A. WILLIAMS 한국생물공학회 2018 한국생물공학회 학술대회 Vol.2018 No.10

Sedimentary characteristics and palaeoclimatic significance of glauberite in Paleocene lacustrine deposits of the Jiangling Depression, central China

Xiaocan Yu,Chenglin Liu,Chunlian Wang,Jiuyi Wang,Haiming Xu,Haonan Li 한국지질과학협의회 2018 Geosciences Journal Vol.22 No.3

The Paleocene sedimentary sequence in the Jiangling Depression was deposited in a salt lake environment. Based on detailed core observation of four drillholes in the Jiangling Depression, a study of sedimentary characteristics of glauberite in Paleocene lacustrine deposits was conducted through thin section petrography, X-ray diffraction, scanning electron microscopy (SEM) and electron probe microanalysis (EPMA). This study has revealed five types of geological occurrences and six types of profile distribution features of glauberite. On the basis of petrological and mineralogical characteristics, we propose that the glauberite is mainly formed by crystallization from saturated sulfate solution and by ion exchange between sulfate solution and calcium-bearing pore solution. Glauberite mainly occurs in the late stage of Paleocene, when brine is relatively highly concentrated. Replacement of gypsum by glauberite is subordinate. The depositional model of glauberite is also discussed. The sulphur isotopic values δ34S) and the strontium ratios (87Sr/86Sr) of the sulphate suggest a non-marine brine supply. The profile distribution features of glauberite contribute to a better understanding of the past climate change. The glauberite in Late Paleocene is frequently interbedded with dolomite, anhydrite, halite or thenardite, suggesting fluctuation of lake water salinity and climate. Trace element compositions of the mudstones imply the reducing condition and fluctuating salinity. The Homogenization temperatures and hydrogen and oxygen isotopic values (δDSMOW and δ18OSMOW) in halite and thenardite inclusions further indicate that in Paleocene the temperature was relatively high and fluctuant. Moreover, the palaeoclimate was drier in the late stage of Late Paleocene, though the temperature was cooler/lower.

The Geochemical and Zircon Trace Element Characteristics of A-type Granitoids in Boziguoer, Baicheng County, Xinjiang

윤경무,유춘화,박정현,소흥곤,양해도,서해명,왕군,Yin, Jingwu,Liu, Chunhua,Park, Jung Hyun,Shao, Xingkun,Yang, Haitao,Xu, Haiming,Wang, Jun The Korean Society of Economic and Environmental G 2013 자원환경지질 Vol.46 No.2

신장 위구르 자치구 바이청현 보즈구얼 지역의 A형 화강암체는 타림지대의 북쪽 끝과 동서쪽 방향의 알칼리 관입암대에 접하여 위치해 있다. 이 화강암체는 추휘석(aegrine)이나 아페소나이트(arfvedsonite)-석영-알칼리장석 섬장암, 또는 아페소나이트-알칼리장석 화강암, 흑운모-알칼리장석 섬장암으로 구성되며, 주요광물로는 알바이트(albite), K-장석, 석영, 아페소나이트(arfvedsonite), 추휘석, 시데로필라이트(siderophyllite) 등이 있으며 부성분 광물로는 지르콘(zircon), 파이로클로르(pyrochlore), 토라이트(thorite), 형석, 모나자이트(monazite), 바스네사이트(bastnaesite), 제노타임(xenotime), 아스트로필라이트(astrophyllite) 등이 있다. 이 알칼리 화강암체는 67.32%의 평균값을 갖는 $SiO_2$를 포함하고 평균 11.14%의 높은 $Na_2O+K_2O$(9.85~11.87%) 조성을 보이며 이 중 $K_2O$는 평균 4.73%의 조성을 보인다. $K_2O/Na_2O$의 비는 0.31~0.96 사이이며 $Al_2O_3$는12.58%부터 15.44%사이로 포함되고 총 $FeO^T$함량은 2.35~5.65%이내이다. CaO, MgO, MnO, $TiO_2$의 함량은 낮은 것으로 관찰된다. 희토류원소의 총량은 평균 $77{\times}10^{-6}$으로 비교적 높게 나타나고 경희토원소는 농축되어 있지만 중희토원소는 Eu원소의 부(-)의 이상과 함께 상대적으로 결핍되는 특징을 가진다. 또한 알칼리 화강암체의 chondrite-normalized 희토류원소의 패턴은 오른쪽으로 기울어진 갈매기형태를 보인다. Zr는 평균 $594{\times}10^{-6}$의 함량을 보이며 Zr+Nb+Ce+Y는 평균 $1362{\times}10^{-6}$의 함량을 나타낸다. 또한 이 알칼리 화강암체는 높은 HFSE(Ga, Nb, Ta, Zr, Hf)함량과 낮은 LILE(Ba, K, Sr)함량을 보인다. Nb/Ta의 비는 평균 16.5이며 Zr/Hf의 비는 평균 36.80이다. 지르콘은 경희토원소에서 결핍되고 중희토원소에서 농축되어있다. 지르콘의 chondrite-normalized 희토류원소는 Eu원소의 강한 부(-)의 이상과 함께 왼쪽으로 기울어진 갈매기형태를 보인다. 보즈구얼지역의 A타입 화강암체는 A1타입의 화강암과 유사한 특징을 나타낸다. 화강암질 마그마의 평균온도는 $832{\sim}839^{\circ}C$이며 보즈구얼 지역의 A타입 화강암체는 지각과 맨틀의 혼합양상을 보이며 고온, 무수, 낮은 산소의 퓨개시티(fugacity) 환경을 가진 판 내부의 비조산대에서 생성되었을 가능성이 있다. The Boziguoer A-type granitoids in Baicheng County, Xinjiang, belong to the northern margin of the Tarim platform as well as the neighboring EW-oriented alkaline intrusive rocks. The rocks comprise an aegirine or arfvedsonite quartz alkali feldspar syenite, an aegirine or arfvedsonite alkali feldspar granite, and a biotite alkali feldspar syenite. The major rock-forming minerals are albite, K-feldspar, quartz, arfvedsonite, aegirine, and siderophyllite. The accessory minerals are mainly zircon, pyrochlore, thorite, fluorite, monazite, bastnaesite, xenotime, and astrophyllite. The chemical composition of the alkaline granitoids show that $SiO_2$ varies from 64.55% to 72.29% with a mean value of 67.32%, $Na_2O+K_2O$ is high (9.85~11.87%) with a mean of 11.14%, $K_2O$ is 2.39%~5.47% (mean = 4.73%), the $K_2O/Na_2O$ ratios are 0.31~0.96, $Al_2O_3$ ranges from 12.58% to 15.44%, and total $FeO^T$ is between 2.35% and 5.65%. CaO, MgO, MnO, and $TiO_2$ are low. The REE content is high and the total ${\sum}REE$ is $(263{\sim}1219){\times}10^{-6}$ (mean = $776{\times}10^{-6}$), showing LREE enrichment HREE depletion with strong negative Eu anomalies. In addition, the chondrite-normalized REE patterns of the alkaline granitoids belong to the "seagull" pattern of the right-type. The Zr content is $(113{\sim}1246){\times}10^{-6}$ (mean = $594{\times}10^{-6}$), Zr+Nb+Ce+Y is between $(478{\sim}2203){\times}10^{-6}$ with a mean of $1362{\times}10^{-6}$. Furthermore, the alkaline granitoids have high HFSE (Ga, Nb, Ta, Zr, and Hf) content and low LILE (Ba, K, and Sr) content. The Nb/Ta ratio varies from 7.23 to 32.59 (mean = 16.59) and the Zr/Hf ratio is 16.69~58.04 (mean = 36.80). The zircons are depleted in LREE and enriched in HREE. The chondrite-normalized REE patterns of the zircons are of the "seagull" pattern of the left-inclined type with strong negative Eu anomaly and without a Ce anomaly. The Boziguoer A-type granitoids share similar features with A1-type granites. The average temperature of the granitic magma was estimated at $832{\sim}839^{\circ}C$. The Boziguoer A-type granitoids show crust-mantle mixing and may have formed in an anorogenic intraplate tectonic setting under high-temperature, anhydrous, and low oxygen fugacity conditions.