Accurate Stitching for Polygonal Surfaces

Zhu, Lifeng,Li, Shengren,Wang, Guoping Society for Computational Design and Engineering 2009 International Journal of CAD/CAM Vol.9 No.1

Various applications, such as mesh composition and model repair, ask for a natural stitching for polygonal surfaces. Unlike the existing algorithms, we make full use of the information from the two feature lines to be stitched up, and present an accurate stitching method for polygonal surfaces, which minimizes the error between the feature lines. Given two directional polylines as the feature lines on polygonal surfaces, we modify the general placement method for points matching and arrive at a closed-form solution for optimal rotation and translation between the polylines. Following calculating out the stitching line, a local surface optimization method is designed and employed for postprocess in order to gain a natural blending of the stitching region.

Erdheim-Chester Disease with Emperipolesis: A Unique Case Involving the Heart

Pengcheng Zhu,Naping Li,Lu Yu,Mariajose Navia Miranda,Guoping Wang,Yaqi Duan 대한암학회 2017 Cancer Research and Treatment Vol.49 No.2

Histiocytosis is an uncommon disease characterized by excessive accumulation of histiocytes. Here, we report a rare case of non-Langerhans-cell histiocytosis in a 51- year-old woman who presented with severe symptoms of pericardial effusion. Radiologic investigation also detected multiple bone (lower limbs, vertebrae, ribs, and ilium) lesions. Resected pericardium showed abundant mono- or multi-nucleated non-foamy histiocytes (CD68+/CD163+/S-100+/CD1/langerin) in a fibroinflammatory background. The histiocytes demonstrated emperipolesis of lymphocytes, a hallmark feature of Rosai-Dorfman disease (RDD). However, molecular analysis revealed a BRAF V600E mutation of the proliferating histiocytes, highlighting the neoplastic features frequently observed in another non-Langerhans-cell histiocytosis known as Erdheim- Chester Disease (ECD). We consider this case to be a unique presentation of ECD harboring some RDD-like cells with emperipolesis, but not a case of RDD with a BRAF mutation concerning its clinical manifestation (involvement of the heart and bones) and neoplastic features.

Microcystic Stromal Tumor of Testicle: First Case Report and Literature Review

Pengcheng Zhu,Yaqi Duan,Qilin Ao,Guoping Wang 대한암학회 2018 Cancer Research and Treatment Vol.50 No.4

Microcystic stromal tumor (MCST) is a rare subtype of sex cord-stromal neoplasm. Tumors from all 31 previously reported cases were located in the ovary. Herein, we present a unique case of a right-side testicular tumor in a 33-year-old Chinese male. The tumor is composed of predominantly lobulated cellular nodules separated by hyalinized fibrous stroma and they expressed CD10, -catenin (nuclear), and cyclin D1. Molecular analysis identified a point mutation (c.110C>G) in exon 3 of CTNNB1. The histopathological features, immunohistochemistry profiles, and molecular analysis of this tumor were consistent with MCST of the ovary. Therefore, a diagnosis of MCST of the right testicle was determined. To the best of our knowledge, this is the first case of MCST occurring in the testicles. The study may provide new insights to the tumor biology of MCST and a better understanding of this rare entity.

Identification and Characterization of Ectoine Biosynthesis Genes and Heterologous Expression of the ectABC Gene Cluster from Halomonas sp. QHL1, a Moderately Halophilic Bacterium Isolated from Qinghai Lake

Derui Zhu,Jian Liu,Rui Han,Guoping Shen,Qifu Long,Xiaoxing Wei,Deli Liu 한국미생물학회 2014 The journal of microbiology Vol.52 No.2

The moderately halophilic bacterium Halomonas sp. QHL1was identified as a member of the genus Halomonas by 16SrRNA gene sequencing. HPLC analysis showed that strainQHL1 synthesizes ectoine in its cytoplasm. The genes involvedin the ectoine biosynthesis pathway were identifiedon the chromosome in the order ectABC. Subsequently, theectB gene from this strain was amplified by PCR, and theentire ectABC gene cluster (3,580 bp) was cloned using genomewalking. Analysis showed that the ectA (579 bp), ectB(1269 bp), and ectC (390 bp) genes were organized in a singletranscriptional unit and were predicted to encode threepeptides of 21.2 kDa, 46.4 kDa, and 14.7 kDa, respectively. Two putative promoters, a δ70-dependent promoter and aδ38-controlled promoter, as well as several conserved motifswith unknown function were identified. Individual ectA, ectB,and ectC genes, and the entire ectABC gene cluster were insertedinto the expression plasmid pET-28a(+) to generatethe recombinant plasmids pET-28a(+)-ectA, pET-28a(+)-ectB, pET-28a(+)-ectC and pET-28a(+)-ectABC, respectively. Heterologous expression of these proteins in Escherichiacoli BL21 (DE3) was confirmed by SDS-PAGE. The recombinantE. coli strain BL21 (pET-28a (+)-ectABC) displayeda higher salt tolerance than native E. coli cells but producedfar less ectoine than the wild-type QHL1 strain.

Alterations of oral microbiota in Chinese children with viral encephalitis and/or viral meningitis

Li Yijie,Liu Jing,Zhu Yimin,Peng Chunying,Dong Yao,Liu Lili,He Yining,Lu Guoping,Zheng Yingjie 한국미생물학회 2022 The journal of microbiology Vol.60 No.4

The role of oral microbiota in viral encephalitis and/or viral meningitis (VEVM) remains unclear. In this hospital-based, frequency-matched study, children with clinically diagnosed VEVM (n = 68) and those with other diseases (controls, n = 68) were recruited. Their oral swab samples were collected and the oral microbiota was profiled using 16S rRNA gene sequencing. The oral microbiota of children with VEVM exhibited different beta diversity metrics (unweighted UniFrac distance: P < 0.001, R2 = 0.025, Bray-curtis dissimilarity: P = 0.045, R2 = 0.011, and Jaccard dissimilarity: P < 0.001, R2 = 0.017) and higher relative abundances of taxa identified by Linear discriminant analysis (LDA) with effect size (Enterococcus, Pedobacter, Massilia, Prevotella_9, Psychrobacter, Butyricimonas, Bradyrhizobium, etc., LDA scores > 2.0) when compared with the control group. The higher pathway abundance of steroid hormone biosynthesis predicted by oral microbiota was suggested to be linked to VEVM (q = 0.020). Further, a model based on oral microbial traits showed good predictive performance for VEVM with an area under the receiver operating characteristic curve of 0.920 (95% confidence interval: 0.834–1.000). Similar results were also obtained between children with etiologically diagnosed VEVM (n = 43) and controls (n = 68). Our preliminary study identified VEVM-specific oral microbial traits among children, which can be effective in the diagnosis of VEVM.

Glasgow prognostic score and combined positive score for locally advanced rectal cancer

Yanru Feng,Jialin Luo,Peng Liu,Luying Liu,Yuan Zhu,Guoping Cheng,Linfeng Zheng 대한외과학회 2022 Annals of Surgical Treatment and Research(ASRT) Vol.102 No.3

Purpose: This study was performed to investigate the association of Glasgow prognostic score (GPS), combined positive score (CPS), and clinicopathological characteristics of locally advanced rectal cancer. Methods: Between February 2012 and February 2018, 103 patients with locally advanced rectal cancer treated by neoadjuvant chemoradiotherapy and total mesorectal excision (TME) were retrospectively evaluated. Results: According to the classification of the GPS, 85 (82.5%), 13 (12.6%), and 5 patients (4.9%) were classified as a score of 0, 1, and 2, respectively. Patients were classified into the GPS-low group (GPS of 0, n = 85) and GPS-high group (GPS of 1 or 2, n = 18) with an area under the curve of 0.582 for overall survival (OS). The mean programmed death-ligand 1 (PD-L1) CPS of the whole group was 2.24 (range, 0–70). The PD-L1 CPS of the GPS-high group was higher than the GPS-low group (P < 0.001). Multivariate analysis by Cox proportional hazards model indicated that GPS was associated with OS and disease- free survival (DFS). Furthermore, PD-L1 CPS was associated with DFS (hazard ratio, 1.050; 95% confidence interval, 1.017– 1.083; P = 0.003). Conclusion: Elevated GPS was related to the PD-L1 CPS. GPS and PD-L1 CPS were associated with the prognosis of locally advanced rectal cancer treated with neoadjuvant chemoradiotherapy followed by TME.

Biochemical Properties and Physiological Roles of NADP-Dependent Malic Enzyme in Escherichia coli

Baojuan Wang,Peng Wang,Enxia Zheng,Xiangxian Chen,Hanjun Zhao,Ping Song,Ruirui Su,Xiaoning Li,Guoping Zhu 한국미생물학회 2011 The journal of microbiology Vol.49 No.5

Malic enzymes catalyze the reversible oxidative decarboxylation of L-malate using NAD(P)^+ as a cofactor. NADP-dependent malic enzyme (MaeB) from Escherichia coli MG1655 was expressed and purified as a fusion protein. The molecular weight of MaeB was about 83 kDa, as determined by SDS-PAGE. The recombinant MaeB showed a maximum activity at pH 7.8 and 46°C. MaeB activity was dependent on the presence of Mn^(2+) but was strongly inhibited by Zn^(2+). In order to understand the physiological roles, recombinant E. coli strains (icdNADP/ΔmaeB and icdNAD/ΔmaeB) containing NADP-dependent isocitrate dehydrogenase (IDH), or engineered NAD-dependent IDH with the deletion of the maeB gene, were constructed using homologous recombination. During growth on acetate, icd^(NAD)/ΔmaeB grew poorly, having a growth rate only 60% that of the wild-type strain (icd^(NADP)). Furthermore, icd^(NAD)P/ΔmaeB exhibited a 2-fold greater adaptability to acetate than icdNAD/ΔmaeB, which may be explained by more NADPH production for biosynthesis in icd^(NAD)P/ΔmaeB due to its NADP-dependent IDH. These results indicated that MaeB was important for NADPH production for bacterial growth on acetate. We also observed that MaeB activity was significantly enhanced (7.83-fold) in icd^(NAD), which was about 3-fold higher than that in icd^(NADP), when switching from glucose to acetate. The marked increase of MaeB activity was probably induced by the shortage of NADPH in icd^(NAD). Evidently, MaeB contributed to the NADPH generation needed for bacterial growth on two carbon compounds.