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( Gun Hyung Na ),( Seunghoon Han ),( Sung Ho Choi ),( Tae Ho Hong ),( Young Kyoung You ),( Dong Goo Kim ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1
Purpose: Hepatitis B virus (HBV) is a leading cause of chronic hepatitis, cirrhosis, and hepatocellular carcinoma in Asia and is responsible for approximately 500,000 deaths per year worldwide. The HBV recurrence rate after LT is greater than 80% without any prophylaxis, and HBV reinfection may lead to rapid disease progression and early graft loss. Prevention of HBV recurrence after LT is essential in HBV-related patients. In recent years, the combination of long-term hepatitis B immunoglobulin (HBIG) and nucleos(t)ide analogues is currently the standard treatment and has effectively reduced HBV recurrence rates. However, there are few studies about the pharmacokinetic characteristics of HBIG. In the present study, we assessed the pharmacokinetic characteristics of HBIG, clinical factors influencing the concentration of HBIG, and the appropriate dose of HBIG. Methods: HBsAg-Positive adult HBV patients who were scheduled to receive liver transplantation followed by preventive immunoglobulin treatment were eligible to the study. All patients were treated with a combination of HBIG and nucleos(t)ide analogues for prophylaxis of HBV recurrence, and were given 10,000 units of HBIG intravenously during the anhepatic phase, which was followed daily for 7 days and then every month for 6 months after LDLT. Whole blood samples were obtained at 30 minutes after the first administration and at predose and 1 hour after the dose of day 1, 7, 28, 84, 128 for pharmacokinetic analysis. Mixed-effect modeling was performed using NONMEM (Ver.7.3, Icon Development Solution, Ellicott City, MD). Results: A total of 228 plasma concentration data were obtained from 20 patients. A 1-compartment model was chosen to explain the dis position of immunoglobulin. The overall concentration in the immediate period after transplantation was affected by the level of viral DNA titer and it was included in the model as a covariate for bioavailability under the assumption that the virus is neutralized immediately after the immunoglobulin dosing. The clearance was highest during the 1 week after transplantation and decreased thereafter. The preoperative clinical factor influencing the concentration of HBIG at day 7 after LT was the level of bilirubin. Currently, the recommended dose for maintenance of immunoglobulin >300 IU/mL is considered to be 3600 IU/month. Conclusion: Pre-operative the level of viral DNA titer and HBeAg are the most influencing virological factors on the concentration of HBIG in the immediate period after LT. The level of bilirubin is the most influencing clinical factors on the concentration of HBIG in the immediate period after LT. The recommended dose for maintenance of immunoglobulin> 300 IU/mL is considered to be 3600 IU/month.
Effects of glucose concentration in the medium on rat hepatocyte culture
Gun Hyung Na,Dong Goo Kim,Young Hui Kim,Jae Hyun Han,Eun Sun Jung 대한외과학회 2014 Annals of Surgical Treatment and Research(ASRT) Vol.87 No.2
Purpose: To determine the optimum culture conditions by investigating isolated rat hepatocytes cultured in medium containing different glucose concentrations. Methods: Hepatocytes were isolated from rats using a two-step perfusion technique and divided into the following two groups cultured in medium containing different glucose concentrations: (1) low-glucose group and (2) high-glucose group. Total cell count and viability of cultured rat hepatocytes and liver function parameters (i.e., concentrations of albumin, ammonia, and urea in the culture medium) were measured. The morphology of cultured rat hepatocytes was examined by staining with hematoxylin and eosin, and albumin receptor expression was confirmed by immunofluorescence. Results: Total cell count and viability showed smaller increases in the low-glucose group than the high-glucose group, although the difference was not statistically significant (P = 0.112 and P = 0.147, respectively). The levels of albumin (P = 0.943), ammonia (P = 0.744), and urea (P = 0.709) were not significantly different between the two groups. In both groups, the function of cultured hepatocytes decreased significantly over time. The morphology of hepatocytes was well maintained in both groups at 3 days. On day 7, the cytoplasm was transformed into a spindle shape. On day 10, these changes were exaggerated, and were more prominent in the high-glucose group. Conclusion: Morphological assessment indicated that low-glucose culture medium is better than high-glucose culture medium for culturing of hepatocytes, although there was not significantly different in functional assessment. The cultured hepatocytes with low-glucose culture medium could be maintained for 7 days.
재발한 위장관 간질성 종양(GISTs) 환자들의 임상양상 분석
나건형(Gun Hyung Na),김영균(Young Kyun Kim),이한홍(Han Hong Lee),지성배(Sung Bae Ji),채병주(Byung Joo Chae),허훈(Hoon Hur),김욱(Wook Kim),전해명(Hae Myung Jeon) 대한외과학회 2007 Annals of Surgical Treatment and Research(ASRT) Vol.73 No.4
Purpose: Gastrointestinal stromal tumors (GISTs) are of a mesenchymal origin, and they arise predominantly from the gastrointestinal tract. This study aims to aid the post operative management of GIST patients by analyzing the clinical and immunopathological features of recurrent GISTs. Methods: We enrolled 98 GIST patients who had been operated on for their primary tumor from 1987 to 2001 at the Catholic Medical Center. Among them, 28 patients had clinical and radiological features that were proved to be recurrence, and the patients’ survival rates were compared according to the treatment modalities. Results: When the maximum length of tumor was shorter and the mitotic index lower, then these patients had higher disease free survival rates and lower recurrence rates. The Ki-67 negative group had lower recurrence rates than their Ki-67 positive counterparts. Recurrence was mostly observed as liver metastasis. The mean length of time to recur was 22.96 months. Fifteen patients received additional treatments such as surgery, chemotherapy and radiation therapy, but there was no significant difference in survival rates when they were compared to the 13 patients who did not receive further treatments. Conclusion: Of the patients diagnosed with GIST after surgery, those with worse prognostic factors, i.e. a bigger tumor size and higher mitotic index, require more meticulous surveillance for tumor recurrence, and especially for liver recurrence, which was the most common recurrence site, during the follow up exams. In addition, although there have not been any remarkably effective treatments for the recurrent GIST patients, further researches for new therapy such as STI-571 is mandated.
Hyung-Gun Kim,Young-Na Huh,Kun-Suk Park 대한생리학회-대한약리학회 1998 The Korean Journal of Physiology & Pharmacology Vol.2 No.6
<P> A reversed-phase high-performance liquid chromatogrphy (RP-HPLC) has been developed to analyze the metabolites of arachidonic acid based on the specificities of ultraviolet absorption of these various metabolites and is sensitive to the nanogram level. This procedure makes it possible to extract complex mixtures of eicosanoids efficiently with a single step and to analyze them simultaneously by RP-HPLC from biological samples using octadesylsilyl silica extraction column and PGB<SUB>2</SUB> as an internal standard. The cyclooxygenase products {prostaglandin (PG)D<SUB>2</SUB>, PGE<SUB>1</SUB>, PGE<SUB>2</SUB>, PGF<SUB>1α</SUB>, PGF<SUB>2α</SUB>, 6-keto-PGF<SUB>1α</SUB>, and thromboxane B<SUB>2 </SUB>(TXB<SUB>2</SUB>)} and lipid peroxidation product, isoprostanes, of arachidonic acid were monitored by one isocratic HPLC system at 195 nm wavelength. The lipoxygenase products {leukotriene(LT)B<SUB>4</SUB>, LTC<SUB>4</SUB>, LTD<SUB>4</SUB>, and 5-hydroxyeicosatetraenoic acid (5-HETE), 12-HETE, 15-HETE} were measured by another isocratic HPLC system at 280 nm for LTs and 235 nm for HETEs. This method provides a simple and reliable way to extract and assess quantitatively the final arachidonic acid metabolites.
Gun Song,Ji-Won Son,Ji-Hyun Jang,Sung-Hyeon Choi,Woo-Hyung Jang,Bin-Na Lee,Chan Park 대한치과보철학회 2021 The Journal of Advanced Prosthodontics Vol.13 No.2
Purpose. This study aims to compare the volumetric change, degree of conversion (DOC), and cytotoxicity of 3D-printed restorations post-cured under three different conditions. Materials and Methods. 3D-printed interim restorations were post-cured under three different conditions and systems: 5 min, 30 min, and 24 h. Three-unit and six-unit fixed dental prostheses (n = 30 for each case) were printed; ten specimens from each group were post-cured and then scanned to compare their volumetric changes. Root-mean-squared (RMS) values of the data were acquired by superimposing the scanned files with original files. Thirty disk-shaped specimens were printed to evaluate the DOC ratio. Fourier transform infrared spectroscopy was used to compare the DOCs of 10 specimens from each group. Human gingival fibroblasts were used to measure the cell viability of every specimen (n = 7). The data from this experiment were employed for one-way analysis of variance and Tukey’s post-hoc comparisons. Results. Differences between the three-unit restorations were statistically insignificant, regardless of the post-curing conditions. However, for the six-unit restorations, a high RMS value was acquired when the post-curing duration was 30 min. The average DOC was approximately 56 - 62%; the difference between each group was statistically insignificant. All the groups exhibited cell viability greater than 70%, rendering them clinically acceptable. Conclusion . The post-curing conditions influenced the volume when the length of the restoration was increased. However, this deviation was found to be clinically acceptable. Additionally, postcuring did not significantly influence the DOC and cytotoxicity of the restorations.