Limiting Concentrate during Growing Period Affect Performance and Gene Expression of Hepatic Gluconeogenic Enzymes and Visfatin in Korean Native Beef Calves

Chang, S.S.,Lohakare, J.D.,Singh, N.K.,Kwon, E.G.,Nejad, J.G.,Sung, K.I.,Hong, S.K. Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.2

This study elucidated the effects of limited concentrate feeding on growth, plasma profile, and gene expression of gluconeogenic enzymes and visfatin in the liver of Hanwoo beef calves. The purpose of this study was to test that reducing the amount of concentrate would partially be compensated by increasing the intake of forage and by altering the metabolic status. The study utilized 20 Korean native beef calves (Hanwoo; 60 to 70 d of age) divided into two groups of 10 calves each for 158 d. Control group calves received the amount of concentrate as per the established Korean feeding standards for Hanwoo, whereas calves in the restricted group only received half the amount of concentrate as per standard requirements. Good quality forage (Timothy hay) was available for ad libitum consumption to both groups. Since calves were with their dam until 4 months of age in breeding pens before weaning, the intake of milk before weaning was not recorded, however, the concentrate and forage intakes were recorded daily. Body weights (BW) were recorded at start and on 10 d interval. Blood samples were collected at start and at 50 d interval. On the final day of the experiment, liver biopsies were collected from all animals in each group. The BW was not different between the groups at all times, but tended to be higher (p = 0.061) only at final BW in control than restricted group. Total BW gain in the control group was 116.2 kg as opposed to 84.1 kg in restricted group that led to average BW gain of 736 g/d and 532 g/d in respective groups, and the differences were significant (p<0.01). As planned, the calves in the control group had higher concentrate and lower forage intake than the restricted group. The plasma variables like total protein and urea were higher (p<0.05) in control than restricted group. The mRNA expressions for the gluconeogenic enzymes such as cytosolic phosphoenol pyruvate carboxykinase (EC 4.1.1.32) and pyruvate carboxylase (EC 6.4.1.1), and visfatin measured by quantitative real-time PCR in liver biopsies showed higher expression (p<0.05) in restricted group than control. Overall, restricting concentrate severely reduced the growth intensity and affected few plasma indices, and gene expression in liver was increased indicating that restricting concentrate in the feeding schemes during early growth for beef calves is not advocated.

Efficacy of Disinfectants against Cytoplasmic Polyhedrosis Virus and Microspordia of Tasar Silkworm, Antheraea mylitta D.

( G. P. Singh ),( Alok Sahay ),( D. K. Roy ),( D. N. Sahay ) 한국잠사학회 2005 International Journal of Industrial Entomology Vol.10 No.1

Efficacy of Wood Ash as an Antiviral Agent against Cytoplasmic Polyhedrosis Virus of Tasar Silkworm, Antheraea mylitta D.

( G. P. Singh ),( D. K. Roy ),( Alok Sahay ),( N. Suryanarayana ) 한국잠사학회 2006 International Journal of Industrial Entomology Vol.13 No.2

The efficacy of wood ash from Terminalia arjuna (arjun) and T. tomentosa (asan) has been tested against virosis of tasar silkworm, Antheraea. mylitta D. The Polyhedral Occlusion Bodies (POBs) of Cytoplasmic Polyhedrosis Virus of A. mylitta (AmCPV) were exposed to the aqueous solution (0.5 to 4%) of wood ash for 5 to 30 minutes. The treated suspension of POBs was orally inoculated once to tasar silkworm larvae after 24 hours of 1 st moult, and larvae reared in indoor on arjun leaves till spinning. The application of aqueous solution of wood ash has established its potential as antiviral agent against cytoplasmic polyhedrosis virus. Two percent aqueous solution of wood ash from arjun and asan dissolved the Polyhedral Occlusion Bodies (POBs) of cytoplasmic polyhedrosis virus of tasar silkworm and inactivated the virions within a short period of 20 to 30 minutes. In vivo efficacy of aqueous solution of wood ash resulted in reduction of larval mortality due to virosis. The mortality was reduced to 2.56±0.2land 3.03±0.32% when treatment of 2.0% solution of wood ash of arjun and asan respectively were applied for 20 minutes, compared to inoculated control (92.18±7.52%). No mortality was recorded when treatment of2.5% solution of wood ash of arjun and asan were applied for 10 minutes or more.

Efficacy of Wood Ash as an Antiviral Agent against Cytoplasmic Polyhedrosis Virus of Tasar Silkworm, Antheraea mylitta D.

Singh, G.P.,Roy, D.K.,Sahay, Alok,Suryanarayana, N. Korean Society of Sericultural Science 2006 International Journal of Industrial Entomology Vol.13 No.2

The efficacy of wood ash from Terminalia arjuna (arjun) and T. tomentosa (asan) has been tested against virosis of tasar silkworm, Antheraea. mylitta D. The Polyhedral Occlusion Bodies (POBs) of Cytoplasmic Polyhedrosis Virus of A. mylitta (AmCPV) were exposed to the aqueous solution (0.5 to 4%) of wood ash for 5 to 30 minutes. The treated suspension of POBs was orally inoculated once to tasar silkworm larvae after 24 hours of $1^{st}$ moult, and larvae reared in indoor on arjun leaves till spinning. The application of aqueous solution of wood ash has established its potential as antiviral agent against cytoplasmic polyhedrosis virus. Two percent aqueous solution of wood ash from arjun and asan dissolved the Polyhedral Occlusion Bodies (POBs) of cytoplasmic polyhedrosis virus of tasar silkworm and inactivated the virions within a short period of 20 to 30 minutes. In vivo efficacy of aqueous solution of wood ash resulted in reduction of larval mortality due to virosis. The mortality was reduced to $2.56{\pm}0.21\;and\;3.03{\pm}0.32%$ when treatment of 2.0% solution of wood ash of arjun and asan respectively were applied for 20 minutes, compared to inoculated control $(92.18{\pm}7.52%)$. No mortality was recorded when treatment of 2.5% solution of wood ash of arjun and asan were applied for 10 minutes or more.

Efficacy of Disinfectants against Cytoplasmic Polyhedrosis Virus and Microspordia of Tasar Silkworm, Antheraea mylitta D.

Singh G. P.,Sahay Alok,Roy D. K.,Sahay D. N. Korean Society of Sericultural Science 2005 International Journal of Industrial Entomology Vol.10 No.1

Bleaching powder solution (1 to $5\%$), slaked lime solution (0.1 to $0.5\%$) and formalin (1 and $2\%$) were tested for their efficacy against cytoplasmic polyhedrosis virus and Nosema mylittansis spores to control virosis and pebrine respectively in tasar silkworm, Antheraea mylitta in indoor rearing condition. All the disinfectants tested were found effective in suppressing the infection of virosis and pebrine significantly. Complete inactivation of Antheraea mylitta cytoplasmic polyhedrosis virus (AmCPV) was recorded when treated with $4\%$ bleaching powder, $0.4\%$ slaked lime for 20 min and $2.0\%$ formalin for 30 min. Similarly treatments of $3.0\%$ bleaching powder solution for 20 min and $2.0\%$ formalin for 30 min were found effective in complete inactivation of N. mylittanis spores.

Eu2+ and Mn2+ Co-doped BaMgAl10O17 Blue- and Green-Emitting Phosphor: A Luminescence and EPR Study

Singh, V.,Sivaramaiah, G.,Rao, J. L.,Singh, N.,Srivastava, A. K.,Jirimali, H. D.,Li, J.,Gao, H.,Kumaran, R. S.,Singh, P. K. Springer Science + Business Media 2016 Journal of electronic materials Vol.45 No.6

<P>Eu2+ and Mn2+ co-doped BaMgAl10O17 phosphor has been prepared by a solution combustion method. The structural, morphological and compositional analysis of the BaMgAl10O17:Eu2+ and Mn2+ powders have been studied by x-ray diffraction, scanning electron microscopy and energy dispersive x-ray spectroscopy. The electron paramagnetic resonance (EPR) spectrum exhibited resonance signals with the effective g values of g a parts per thousand 4.88 and g a parts per thousand 1.98. The resonance signal with the effective g value of g a parts per thousand 4.88 is characteristic of Eu2+ ions whereas g a parts per thousand 1.98 is due to Mn2+ ions. The number of spins participating in resonance, Gibbs free energy, magnetic susceptibility, Curie constant, effective magnetic moment, zero-field splitting parameter and hyperfine splitting constant have been evaluated. From optical and EPR correlation, it is inferred that Eu2+ and Mn2+ are present in the BaMgAl10O17 matrix.</P>

Cr3+-Doped Yb3Ga5O12 Nanophosphor: Synthesis, Optical, EPR, Studies

Singh, V.,Sivaramaiah, G.,Rao, J. L.,Singh, N.,Pathak, M. S.,Jirimali, H. D.,Singh, P. K.,Srivastava, A. K.,Dhoble, S. J.,Mohapatra, M. Springer Science + Business Media 2016 Journal of electronic materials Vol.45 No.8

<P>Gallium garnets of lanthanides are multifunctional materials especially known for their complicated structure and magnetic properties. In addition, with a suitable transition metal dopant ion, these matrices have been proved to be excellent materials for lasers. In particular, gallium garnet of ytterbium (Yb3Ga5O12) is known to possess excellent properties with regards to these applications. In this connection, Yb3Ga5O12 doped with Cr3+ nanophosphors were synthesized by a solution combustion route. The synthesized material was characterized by powder x-ray diffraction and scanning electron microscopy for phase purity and homogenous morphology. In order to ascertain the oxidation state of the doped ion, diffuse reflectance (DRF), photoluminescence (PL) and electron paramagnetic resonance (EPR) experiments were performed on the sample. The DRF and PL data suggested the stabilisation of the trivalent Cr ion in the matrix. The EPR spectra exhibited two resonance signals with effective g values at g approximate to 7.6 and 4. The EPR data corroborated the DRF and PL results, suggesting the stabilisation of Cr3+ in the matrix at octahedral-type geometries.</P>

EPR and optical investigation of ultraviolet-emitting Gd3Ga5O12 garnet

Singh, Vijay,Sivaramaiah, G.,Singh, N.,Mohapatra, M.,Hakeem, D. A.,Pathak, M. S.,Rao, J. L. Springer-Verlag 2018 Journal of materials science Materials in electron Vol.29 No.2

<P>Gd3Ga5O12 garnet was prepared by a solution combustion method and characterized using powder X-ray diffraction, electron paramagnetic resonance (EPR) and optical spectroscopic techniques. EPR spectrum of the samples at ambient and low temperatures exhibited resonance signal at g approximate to 2 attributed to Gd3+ ions disposed in an octahedral symmetry. The optical absorption spectrum showed a band centered at 274 nm attributed to S-8(7/2)-> I-6(J) transition of the Gd3+ ions. The excitation spectrum showed a maximum at 273 nm along with two relatively weaker peaks at 276 and 279 nm. These three excitation bands were assigned to the transitions S-8(7/2)-> I-6(J). The emission spectrum showed two peaks centered at 311 and 306 nm that were assigned to the transitions P-6(7/2)-> S-8(7/2) and P-6(5/2)-> S-8(7/2) of Gd3+, respectively. To evaluate the phosphor performance, several magnetic and thermodynamic parameters for the system were calculated adopting standard procedure.</P>

EPR and PL studies on UVB-emitting gadolinium-doped SrAl₁₂O₁₉ phosphors

Singh, Vijay,Sivaramaiah, G.,Singh, N.,Rao, J.L.,Singh, Pramod K.,Pathak, M.S.,Hakeem, D.A. Elsevier 2018 Optik Vol.158 No.-

<P><B>Abstract</B></P> <P>A series of UV-emitting Gd-doped SrAl<SUB>12</SUB>O<SUB>19</SUB> phosphors were prepared using a low-temperature solution-combustion method for which urea served as the fuel. These phosphors were characterized using X-ray diffraction (XRD), electron paramagnetic resonance (EPR), and photoluminescence (PL) studies. In phosphors with low dopant concentrations, the EPR spectrum exhibited resonance signals at the effective g factors of 2.41, 2.17, 1.99, 1.88, and 1.66. With the increasing of the concentrations, the resonance signals are evident at the effective g factors of 2.39, 2.18, 1.95, and 1.65. The PL studies of the samples showed a dominant emission peak at 312 nm that corresponds to the <SUP>6</SUP>P<SUB>7/2</SUB> → <SUP>8</SUP>S<SUB>7/2</SUB> transitions of the Gd<SUP>3+</SUP> ions.</P>

Characteristics and Variability of Carbonaceous Aerosols over a Semi Urban Location in Garhwal Himalayas

K. Sandeep,R. S. Negi,A. S. Panicker,Alok Sagar Gautam,D. S. Bhist,G. Beig,B. S. Murthy,R. Latha,Santosh Singh,S. Das 한국기상학회 2020 Asia-Pacific Journal of Atmospheric Sciences Vol.56 No.3

Extraction of organic carbon (OC) and elemental carbon (EC) were carried out over Srinagar, India, an ecologically sensitive semi-urban site in Garhwal Himalays. The PM2.5 sampling was carried out during January to December, 2017 over the site. The OC and EC were extracted from PM2.5 samples using a thermo optical OC/EC analyzer. Highest OC and EC concncentrations were found during postmonsoon (17.67 ± 1.1 μg/m3 OC and 6.34 ± 0.75 EC) and Winter (17.31 ± 3.045 μg/m3 OC and 6.32 ± 0.585 μg/m3 EC) seasons are attributed to boundary layer dynamics and anthropogenic activities. The lower concentration of OC/ EC was observed during monsoon season (11.64 ± 1.75 μgm−3 OC and 3.46 ± 0.19μgm−3 EC) owing to wet scavenging of aerosols and minimum count of forest fire/biomass buring incidences. Both pre-monsoon and post-monsoon season concentrations are also influenced by biomass burning in the IGP (Indo-Gangetic Plain) region and forest fires in the adjecent areas. The OC/EC ratio sounds that vehicular exhaust and biomass burning are the major source of OC/EC over the site. Generation of secondary organic carbon (SOC) at the region causes variability in OC/EC ratio in different seasons. It is found that 24–32% of PM2.5 is contributed by carbonaceous aerosols (OC and EC) over Srinagar. The pivotal role of meteorology in modulating OC/ EC concentrations has been illustared in detail.